公开(公告)号：US11473119B2

公开(公告)日：2022-10-18

申请号：US16656391

申请日：2019-10-17

申请人： Jiangnan University

发明人： Shuo Wang ,  Xiaodong Wang ,  Hui Lv ,  Ji Li ,  Yan Wang

IPC分类号： C12Q1/02 ,  G01N15/14 ,  G01N15/10

摘要： The present disclosure discloses to a method for determining optimum preservation temperature of a sulfur autotrophic denitrifying bacteria biofilm, and belongs to the technical field of environment engineering. The method of the present disclosure comprises: determining the cell activity state of a sulfur autotrophic denitrifying bacteria biofilm preserved at different temperatures by flow cytometry, and determining the preservation temperature of the cell activity state closest to the cell activity state of the sulfur autotrophic denitrifying bacteria in pilot operation as the optimum preservation temperature. The cell activity state and performance effect are verified to be reliable after activity recovery by the test data. The method of the present disclosure can simplify the microbial activity recovery process of the sulfur autotrophic denitrifying bacteria biofilm, quickly start the sulfur autotrophic denitrifying bacteria biofilm sewage treatment, enable the removal rate of nitrate nitrogen and total nitrogen in a sewage treatment plant to reach 96% and 88% or above respectively, achieve the effects of energy saving and consumption reduction at the same time, and have very high industrial feasibility.

公开(公告)号：US20220326155A1

公开(公告)日：2022-10-13

申请号：US17623244

申请日：2021-11-11

申请人： JIANGNAN UNIVERSITY

发明人： Yi ZHANG ,  Yongxi HE ,  Wenlong WANG ,  Lingling WANG ,  Xiuping YAN

IPC分类号： G01N21/64 ,  G01N33/533 ,  G01N33/577 ,  G01N33/58

摘要： A time-resolved fluorescence and chromogenic dual-signal test strip for estrogen is based on the principle of immune recognition and fluorescence resonance energy transfer. In time-resolved fluorescence mode, estrogen-BSA-persistent luminescence particle complex is the fluorescence donor, and colloidal gold modified with estrogen monoclonal antibody is the fluorescence receptor, which is also chromogenic signal unit in the chromogenic mode. The photos of strips in both modes are obtained with smart phones. The time-resolved fluorescence intensity of the test strip test zone is positively correlated with estrogen content, and the chromogenic intensity is negatively correlated with estrogen content. The competitive time-resolved fluorescence and chromogenic dual-signal immunochromatographic test strips can accurately and quickly detect estrogen and estrogen-like compounds.

公开(公告)号：US20220315711A1

公开(公告)日：2022-10-06

申请号：US17846269

申请日：2022-06-22

申请人： Jiangnan University

发明人： Jing HU ,  Jian YIN ,  Xiang WANG

IPC分类号： C08J3/075 ,  C12N5/077 ,  C08J3/24 ,  C08K3/26

摘要： The present disclosure discloses a preparation method and use of a crosslinked hydrogel for muscle stem cell culture, and belongs to the technical field of biological food materials. Chitosan, alginate, dextran and Ca2+ are crosslinked through physical crosslinking to form a double-network hydrogel with a high mechanical strength, the hydrogel is coated with heparin and collagen through dip coating, such that the hydrogel can immobilize growth factors and adhere to cells. Meanwhile, extracted primary muscle stem cells are inoculated onto the hydrogel and cultured in a growth medium (79% of DMEM, 10% of FBS and 1% of double antibodies) for 24 h. The cells are cultured in an incubator with a differential medium (97% of DMEM, 2% of horse serum and 1% of double antibodies) for 7 d. The hydrogel can enhance the absorption to nutrient substances by the muscle stem cells and facilitate growth of the muscle stem cells. The double-network hydrogel has the potential to be a scaffold for growth of muscle stem cells for cultured meat from stem cells.

公开(公告)号：US11445728B2

公开(公告)日：2022-09-20

申请号：US17549982

申请日：2021-12-14

申请人： Jiangnan University

发明人： Zhenyu Wang ,  Zhenggao Xiao ,  Chuanxi Wang ,  Le Yue

IPC分类号： A01N59/00 ,  C09K11/59 ,  C01B33/021 ,  A01N25/04 ,  B82Y20/00 ,  B82Y40/00 ,  B82Y5/00

摘要： The present disclosure discloses an application method of silicon quantum dots (Si QDs) for controlling corn armyworm, belonging to the technical field of nano-agricultural technology for crop pest control. The method for controlling corn armyworm by using Si QDs of the present disclosure includes the following steps of: preparing the Si QDs into an aqueous solution of the Si QDs, and then applying the aqueous solution on the roots or leaves of plants as plant fertilizer; where the concentration of the aqueous solution of the Si QDs is 10-150 mg/L; the size of the Si QDs is 3-8 nm. The method of the present disclosure not only improves the effect of the conventional silicon fertilizer on the stress resistance of plants, but also directly improves the direct insecticidal effect of the nano-silicon and improves the chemical defense capability of plants; the optimal spraying amount of Si QDs is determined by spraying different concentrations of Si QDs in the growth and development experiment of armyworm.

公开(公告)号：US11441876B2

公开(公告)日：2022-09-13

申请号：US16689112

申请日：2019-11-20

申请人： Jiangnan University

发明人： Kejing Yu ,  Shen Li ,  Kun Qian ,  Diantang Zhang ,  Yang Xu ,  Qingsheng Liu ,  Feng Xu

IPC分类号： F41H5/04 ,  B32B5/02 ,  B32B5/26 ,  B32B5/24 ,  B32B5/18 ,  B32B9/00 ,  B32B9/04 ,  B32B37/02 ,  B32B37/10 ,  B32B37/18 ,  B32B37/14

摘要： A multilayered bullet resistant member, including a three-layered structure formed of a metal-ceramic crack arrest reflecting layer, a fiber-elastomer composite energy absorbing layer and a two-dimensional fabric blunt trauma protective layer sequentially overlapped with each other. The integration of performances of all the components guarantees high strength, hardness and good impact toughness of the bullet resistant member.

公开(公告)号：US11441133B2

公开(公告)日：2022-09-13

申请号：US15734969

申请日：2019-11-22

申请人： JIANGNAN UNIVERSITY

发明人： Ye Ni ,  Zhe Dou ,  Guochao Xu

IPC分类号： C12P7/40 ,  C12N9/18

摘要： The invention discloses a strain of Acinetobacter and use thereof in the production of chiral 3-cyclohexene-1-carboxylic acid. Its taxonomic name is Acinetobacter sp., which is deposited on Jan. 21, 2019 at the China General Microbiological Culture Collection Center, under accession number CGMCC No. 17220. Using the Acinetobacter strain of the invention to produce chiral methyl 3-cyclohexene-1-carboxylate, the resulting methyl (S)-3-cyclohexene-1-carboxylate has an optical purity of 99% or more, and the catalyst has good stability, mild reaction condition and can withstand high concentrations of substrate and product. Using the resolution process of the invention, (S)-3-cyclohexene-1-carboxylic acid with high optical purity and high concentration can be simply and efficiently obtained, and the process is energy-saving and environmentally friendly, and the high-concentration of product is beneficial to downstream product recovery process. The invention provides an efficient method for production of (S)-3-cyclohexene-1-carboxylic acid, and has a good industrial application prospect.

公开(公告)号：US11410035B2

公开(公告)日：2022-08-09

申请号：US16885450

申请日：2020-05-28

申请人： Jiangnan University

发明人： Wei Fang ,  Peiming Ren ,  Lin Wang ,  Jun Sun ,  Xiaojun Wu

IPC分类号： G06N3/08 ,  G06K9/62 ,  G06N3/04

摘要： Disclosed is a real-time object detection method deployed on a platform with limited computing resources, which belongs to the field of deep learning and image processing. In the present invention, YOLO-v3-tiny neural network is improved, Tinier-YOLO reserves the front five convolutional layers and pooling layers of YOLO-v3-tiny and makes prediction at two different scales. Fire modules in SqueezeNet, 1×1 bottleneck layers, and dense connection are introduced, so that the structure is used to achieve smaller, faster, and more lightweight network that can be run in real time on an embedded AI platform. The model size of Tinier-YOLO in the present invention is only 7.9 MB, which is only ¼ of 34.9 MB of YOLO-v3-tiny, and ⅛ of YOLO-v2-tiny. The reduction in the model size of Tinier-YOLO does not affect real-time performance and accuracy of Tinier-YOLO. Real-time performance of Tinier-YOLO in the present invention is 21.8% higher than that of YOLO-v3-tiny and 70.8% higher than that of YOLO-v2-tiny. Compared with YOLO-v3-tiny, accuracy of Tinier-YOLO is increased by 10.1%. Compared with YOLO-v2-tiny, accuracy of Tinier-YOLO is increased by nearly 18.2%. Tinier-YOLO in the present invention can still achieve real-time detection on a platform with limited resources, and effects are better.

公开(公告)号：US20220220244A1

公开(公告)日：2022-07-14

申请号：US17604270

申请日：2019-10-17

申请人： JIANGNAN UNIVERSITY

发明人： Ren LIU ,  Xiucheng ZOU

IPC分类号： C08F283/00 ,  C08F2/48 ,  C08K5/3417 ,  C08K3/04

摘要： A dark-colored system photopolymerization composition, comprising: 20-80 phr of photopolymerizable prepolymer, 5-60 phr of photopolymerizable monomer, 0.2-8 phr of photoinitiator, and 0.1-5 phr of melanin, 0.05-5 phr of upconversion materials. The composition can construct photopolymerization under near-infrared irradiation. The dark pigment can avoid the strong absorption of near-infrared light, and the upconversion material can absorb near-infrared light with good penetrating ability and emit ultraviolet or visible light to induce the decomposition of free radicals or ionic photoinitiators in the composition. Then produced active species realize the photopolymerization of dark-colored compositions. The invention increases the depth of photopolymerization of dark-colored compositions and improves the mechanical properties of the polymer, that will broaden application fields of photopolymerization materials.

公开(公告)号：US11384349B2

公开(公告)日：2022-07-12

申请号：US17104122

申请日：2020-11-25

申请人： Jiangnan University

发明人： Juan Zhang ,  Zheng Peng ,  Jian Chen ,  Guocheng Du ,  Xinzhe Mao ,  Hengrui Zhou

IPC分类号： C12N9/54 ,  C12N1/20 ,  C12R1/125

摘要： The disclosure discloses a recombinant Bacillus subtilis engineered bacterium capable of efficiently expressing keratinase, and belongs to the technical fields of genetic engineering and fermentation engineering. The disclosure successfully constructs a genetically engineered bacterium B. subtilis WB600-pP43NMK-ker capable of efficiently expressing keratinase by using a keratinase gene from Bacillus licheniformis (BBE11-1) as a target gene, pP43NMK as an expression vector and B. subtilis WB600 as an expression host; and meanwhile, the disclosure conducts in-depth research on fermentation media and fermentation conditions when the engineered bacteria are configured as a production strain to produce keratinase to obtain a fermentation medium capable of increasing a yield of keratinase and an optimum process for fermentation production of keratinase.

公开(公告)号：US11384109B2

公开(公告)日：2022-07-12

申请号：US17506985

申请日：2021-10-21

申请人： Jiangnan University

发明人： Xiulan Sun ,  Yida Xu ,  Deping Xu ,  Yinzhi Zhang

IPC分类号： C07H1/08 ,  A61K31/704 ,  C07H15/203 ,  A61K31/7034 ,  A61K36/52

摘要： The disclosure discloses a method for separating phenolic acid glucoside compounds from Diaphragma juglandis Fructus, belonging to the technical field of food. The method includes the following steps: (1) extraction: crushing Diaphragma juglandis Fructus, and then extracting by using ethanol to obtain walnut ethanol extract; and (2) separation: performing column chromatography on the walnut ethanol extract obtained in step (1), sequentially eluting by using ethanol of different concentrations, purifying and eluting repeatedly, and performing separation to obtain phenolic acid glucoside compounds. The phenolic acid glucoside compounds are 2-hydroxyl-5-methoxylphenyl-O-β-D-glucopyranoside, 6-hydroxylnaphthyl-1,2-di-O-β-D-glucopyranoside and 2,6-dihydroxynaphthyl-O-β-D-glucopyranoside. Pharmacological experiments confirm that the three compounds have good antioxidant activity.