公开(公告)号：US20020119927A1

公开(公告)日：2002-08-29

申请号：US09972757

申请日：2001-10-09

Applicant: Myriad Genetics, Inc.

Inventor： Jean-Marc Roch ,  Paul L. Bartel ,  Karen Heichman

IPC: A61K039/395 ,  A61K038/17

CPC classification number: G01N33/6896 ,  A61K38/1709 ,  C07K14/47 ,  C07K14/4711 ,  C07K16/18 ,  C07K16/2833 ,  C07K2317/32 ,  C12Q1/6883 ,  C12Q1/6897 ,  C12Q2600/156 ,  G01N2500/00 ,  G01N2500/02 ,  G01N2800/28 ,  C12Q2565/201

Abstract: The present invention relates to the discovery of protein-protein interactions that are involved in the pathogenesis of neurodegenerative disorders, including Alzheimer's disease (AD). Thus, the present invention is directed to complexes of these proteins and/or their fragments, antibodies to the complexes, diagnosis of neurodegenerative disorders (including diagnosis of a predisposition to and diagnosis of the existence of the disorder), drug screening for agents which modulate the interaction of proteins described herein, and identification of additional proteins in the pathway common to the proteins described herein.

公开(公告)号：US20020119498A1

公开(公告)日：2002-08-29

申请号：US09990762

申请日：2001-11-14

Inventor： J. Keith Joung ,  Jeffrey Miller ,  Carl O. Pabo

IPC: G01N033/53 ,  G01N033/567 ,  C12Q001/68

CPC classification number: C12N15/1055 ,  C12Q1/6897 ,  G01N33/50 ,  G01N33/5008 ,  G01N33/502 ,  C12Q2565/201

Abstract: The present invention provides methods and compositions for interaction trap assays for detecting protein-protein, protein-DNA, or protein-RNA interactions. The methods and compositions of the invention may also be used to identify agents which may agonize or antagonize a protein-protein, protein-DNA, or protein-RNA interaction. In certain embodiments, the interaction trap system of the invention is useful for screening libraries with greater than 107 members. In other embodiments, the interaction trap system of the invention is used in conjunction with flow cytometry. The invention further provides a means for simultaneously screening a target protein or nucleic acid sequence for the ability to interact with two or more test proteins or nucleic acids.

Abstract translation: 本发明提供了用于检测蛋白质 - 蛋白质，蛋白质-DNA或蛋白质 - RNA相互作用的相互作用阱测定法的方法和组合物。 本发明的方法和组合物还可用于鉴定可能激动或拮抗蛋白质 - 蛋白质，蛋白质-DNA或蛋白质 - RNA相互作用的试剂。 在某些实施方案中，本发明的相互作用捕获系统可用于筛选具有大于107个成员的文库。 在其它实施方案中，本发明的相互作用阱系统结合流式细胞术使用。 本发明还提供了用于同时筛选靶蛋白或核酸序列以获得与两种或多种测试蛋白或核酸相互作用的能力的方法。

公开(公告)号：US20020115607A1

公开(公告)日：2002-08-22

申请号：US09975072

申请日：2001-10-12

Applicant: Myriad Genetics, Inc.

Inventor： Jean-Marc Roch ,  Paul L. Bartel ,  Karen Heichman

IPC: A61K038/17 ,  A61K039/395 ,  C12N009/64 ,  C07K014/435 ,  C12N009/12

CPC classification number: G01N33/6896 ,  A61K38/1709 ,  C07K14/47 ,  C07K14/4711 ,  C07K16/18 ,  C07K16/2833 ,  C07K2317/32 ,  C12Q1/6883 ,  C12Q1/6897 ,  C12Q2600/156 ,  G01N2500/00 ,  G01N2500/02 ,  G01N2800/28 ,  C12Q2565/201

Abstract: The present invention relates to the discovery of protein-protein interactions that are involved in the pathogenesis of neurodegenerative disorders, including Alzheimer's disease (AD). Thus, the present invention is directed to complexes of these proteins and/or their fragments, antibodies to the complexes, diagnosis of neurodegenerative disorders (including diagnosis of a predisposition to and diagnosis of the existence of the disorder), drug screening for agents which modulate the interaction of proteins described herein, and identification of additional proteins in the pathway common to the proteins described herein.

公开(公告)号：US20020114799A1

公开(公告)日：2002-08-22

申请号：US09973077

申请日：2001-10-10

Applicant: Myriad Genetics, Inc.

Inventor： Jean-Marc Roch ,  Paul L. Bartel ,  Karen Heichman

IPC: A61K039/395

CPC classification number: G01N33/6896 ,  A61K38/1709 ,  C07K14/47 ,  C07K14/4711 ,  C07K16/18 ,  C07K16/2833 ,  C07K2317/32 ,  C12Q1/6883 ,  C12Q1/6897 ,  C12Q2600/156 ,  G01N2500/00 ,  G01N2500/02 ,  G01N2800/28 ,  C12Q2565/201

Abstract: The present invention relates to the discovery of protein-protein interactions that are involved in the pathogenesis of neurodegenerative disorders, including Alzheimer's disease (AD). Thus, the present invention is directed to complexes of these proteins and/or their fragments, antibodies to the complexes, diagnosis of neurodegenerative disorders (including diagnosis of a predisposition to and diagnosis of the existence of the disorder), drug screening for agents which modulate the interaction of proteins described herein, and identification of additional proteins in the pathway common to the proteins described herein.

公开(公告)号：US06316223B1

公开(公告)日：2001-11-13

申请号：US09359081

申请日：1999-07-22

Applicant: Donald Payan ,  Ying Luo ,  Betty Huang

Inventor： Donald Payan ,  Ying Luo ,  Betty Huang

IPC: C12P2106

CPC classification number: C12N15/1055 ,  C12Q2565/201

Abstract: The present invention is directed to compositions and methods for a genetic system of detecting protein-protein interactions in a mammalian host cell. Two fusion proteins are made in the host cell. The first fusion protein contains a DNA binding domain which is fused to a so-called bait protein. The second fusion protein consists of a transcriptional activation domain fused to a so-called test protein. The DNA binding domain binds to an operator sequence which controls expression of one or more reporter genes. The transcriptional activation domain is recruited to the promoter through the functional interaction between the bait protein and the test protein. Subsequently the transcriptional activation domain interacts with the basal transcription machinery to activate expression of one or more reporter genes which can be identified and characterized. The individual compositions are useful for analyzing protein-protein interactions between known proteins and to isolate, clone and characterize unknown proteins. The individual compositions can be used to express the fusion proteins either transiently or stably. In addition, the present invention is directed to methods for screening for candidate bioactive agents that modulate the protein-protein interaction between a bait protein and a test protein and thus are useful for the identification of novel therapeutic drugs and the like.

Abstract translation: 本发明涉及用于在哺乳动物宿主细胞中检测蛋白质 - 蛋白质相互作用的遗传系统的组合物和方法。 在宿主细胞中制备两种融合蛋白。 第一个融合蛋白含有与所谓诱饵蛋白融合的DNA结合结构域。 第二种融合蛋白由与所谓的测试蛋白融合的转录激活结构域组成。 DNA结合域与控制一个或多个报道基因表达的操纵子序列结合。 通过诱饵蛋白质和测试蛋白质之间的功能相互作用将转录激活结构域引入启动子。 随后，转录激活结构域与基础转录机制相互作用以激活一种或多种可鉴定和表征的报告基因的表达。 单独的组合物可用于分析已知蛋白质之间的蛋白质 - 蛋白质相互作用并分离，克隆和表征未知蛋白质。 单独的组合物可用于瞬时或稳定地表达融合蛋白。 此外，本发明涉及筛选调节诱饵蛋白质和测试蛋白质之间的蛋白质 - 蛋白质相互作用的候选生物活性剂的方法，因此可用于鉴定新的治疗药物等。

公开(公告)号：US20090253131A1

公开(公告)日：2009-10-08

申请号：US12265558

申请日：2008-11-05

Applicant: Susan Wigdal ,  Keith V. Wood

Inventor： Susan Wigdal ,  Keith V. Wood

IPC: C12Q1/68 ,  G01N33/573 ,  C12N9/04 ,  C12N5/00 ,  C12N15/63

CPC classification number: C12Q1/66 ,  C12Q1/6897 ,  C12Q2565/201

Abstract: The invention provides vectors encoding hybrid fusion proteins and vector sets encoding different hybrid fusion proteins useful, for instance, in protein complementation assays.

Abstract translation: 本发明提供了编码杂交融合蛋白的载体和编码不同杂合融合蛋白的载体集，其可用于例如蛋白质互补测定。

公开(公告)号：US07527942B2

公开(公告)日：2009-05-05

申请号：US10503642

申请日：2003-02-07

Applicant: Michael F. Carey ,  Lily Wu ,  Sanjiv Gambhir ,  Meera Iyer ,  Joanne Zhang

Inventor： Michael F. Carey ,  Lily Wu ,  Sanjiv Gambhir ,  Meera Iyer ,  Joanne Zhang

IPC: C12P21/02

CPC classification number: A01K67/0271 ,  A01K2267/0331 ,  C12N2799/022 ,  C12N2800/40 ,  C12N2830/008 ,  C12Q1/6897 ,  C12Q2565/201

Abstract: The present invention provides a transcription amplification system, comprising an effector nucleic acid molecule and a reporter molecule, which work together to produce a heterologous gene product in a cell-type, specific manner. The present invention also relates to methods for producing, detecting, imaging, and monitoring the heterologous gene product in a cell or in a subject.

Abstract translation: 本发明提供了一种转录扩增系统，其包含效应核酸分子和报告分子，它们一起工作以产生细胞型特异性方式的异源基因产物。 本发明还涉及用于在细胞或受试者中产生，检测，成像和监测异源基因产物的方法。

公开(公告)号：US20080261241A1

公开(公告)日：2008-10-23

申请号：US11685122

申请日：2007-03-12

Applicant: Rohde John Roy ,  Rene Raoul Parsot Claude ,  Sansonetti Phillippe Joseph

Inventor： Rohde John Roy ,  Rene Raoul Parsot Claude ,  Sansonetti Phillippe Joseph

IPC: G01N33/53 ,  C12N15/11 ,  C12N15/00 ,  C12N5/06 ,  C12N5/04 ,  C12N1/14 ,  C12N9/00 ,  C12P21/04 ,  C12Q1/00

CPC classification number: C12Y603/02019 ,  C12N9/93 ,  C12Q2565/201 ,  G01N33/5023

Abstract: Members of the IpaH superfamily constitute a novel class of E3 ubiquitin ligases which are useful for engineering products which modulate trafficking and destruction of target proteins inside a cell and useful targets for identifying new antimicrobial molecules which modulate, especially inhibit, E3 ligases.

Abstract translation: IpaH超家族的成员构成了E3泛素连接酶的新一类，其可用于调节细胞内靶蛋白的运输和破坏的工程产物，并且用于鉴定调节，特别是抑制E3连接酶的新抗微生物分子的有用靶标。

公开(公告)号：US20080167259A1

公开(公告)日：2008-07-10

申请号：US11821812

申请日：2007-06-25

Applicant: John L. Teem

Inventor： John L. Teem

IPC: A61K31/70 ,  C12Q1/68 ,  C12N5/06 ,  A61P43/00

CPC classification number: G01N33/564 ,  C07K14/4712 ,  C07K2319/00 ,  C12N15/1055 ,  C12Q1/6895 ,  C12Q1/6897 ,  C12Q2565/201

Abstract: The subject invention concerns materials and methods for detecting the interaction of CFTR proteins. In one embodiment, the method can be used to determine whether one CFTR polypeptide interacts with a second CFTR polypeptide. The subject invention also concerns materials and methods for screening for drugs or compositions that can restore or enhance interaction of CFTR proteins containing mutation(s) that reduce or prevent dimerization of the proteins. The assay of the present invention can be used to screen a large number of compounds in a high throughput format. The subject invention also pertains to host cells useful in the methods of the invention. The subject invention also concerns compositions and methods for treating patients afflicted with cystic fibrosis.

Abstract translation: 本发明涉及用于检测CFTR蛋白质相互作用的材料和方法。 在一个实施方案中，该方法可用于确定一种CFTR多肽是否与第二CFTR多肽相互作用。 本发明还涉及用于筛选能够还原或增强含有减少或阻止蛋白质二聚化的突变的CFTR蛋白质相互作用的药物或组合物的材料和方法。 本发明的测定可用于以高通量形式筛选大量化合物。 本发明还涉及可用于本发明方法的宿主细胞。 本发明还涉及用于治疗患有囊性纤维化的患者的组合物和方法。

公开(公告)号：US07238474B1

公开(公告)日：2007-07-03

申请号：US10089875

申请日：2000-10-06

Applicant: John L. Teem

Inventor： John L. Teem

IPC: C12Q1/68 ,  G01N33/567 ,  C07H21/02 ,  C07H21/04 ,  C12N15/63

CPC classification number: G01N33/564 ,  C07K14/4712 ,  C07K2319/00 ,  C12N15/1055 ,  C12Q1/6895 ,  C12Q1/6897 ,  C12Q2565/201

Abstract: The subject invention concerns materials and methods for detecting the interaction of CFTR proteins. In one embodiment, the method can be used to determine whether one CFTR polypeptide interacts with a second CFTR polypeptide. The subject invention also concerns materials and methods for screening for drugs or compositions that can restore or enhance interaction of CFTR proteins containing mutation(s) that reduce or prevent dimerization of the proteins. The assay of the present invention can be used to screen a large number of compounds in a high throughput format. The subject invention also pertains to host cells useful in the methods of the invention. The subject invention also concerns compositions and methods for treating patients afflicted with cystic fibrosis.

Abstract translation: 本发明涉及用于检测CFTR蛋白质相互作用的材料和方法。 在一个实施方案中，该方法可用于确定一种CFTR多肽是否与第二CFTR多肽相互作用。 本发明还涉及用于筛选能够还原或增强含有减少或阻止蛋白质二聚化的突变的CFTR蛋白质相互作用的药物或组合物的材料和方法。 本发明的测定可用于以高通量形式筛选大量化合物。 本发明还涉及可用于本发明方法的宿主细胞。 本发明还涉及用于治疗患有囊性纤维化的患者的组合物和方法。