公开(公告)号：US20100282610A1

公开(公告)日：2010-11-11

申请号：US12812466

申请日：2009-01-19

申请人： Gunnar Glad ,  Nils Norrman ,  Susanna Lindman

发明人： Gunnar Glad ,  Nils Norrman ,  Susanna Lindman

IPC分类号： B01D57/02 ,  C07K1/14

CPC分类号： G01N27/44743 ,  B01L3/5082 ,  B01L2400/0421 ,  B01L2400/043 ,  G01N1/405 ,  G01N2446/00

摘要： The present invention relates to a method for sample application and separation. More closely, the invention relates to convenient direct loading of a biomolecule sample via magnetic beads to, for example, a gel before electrophoresis. In this way, the invention combines elution and application steps with minimal losses of sample. Thus, the invention relates to a method for sample application of biomolecules on a separation media, comprising the following steps: a) obtaining said biomolecules from a sample by magnetic beads; b) applying the magnetic beads with the biomolecules to a separation medium; c) releasing the biomolecules into the separation media, and d) separation of the biomolecules from each other in the separation medium.

摘要翻译： 本发明涉及一种样品应用和分离方法。 更为详细地，本发明涉及通过磁珠将生物分子样品直接加载到例如电泳前的凝胶。 以这种方式，本发明将洗脱和应用步骤组合在一起，同时具有最小的样品损失。 因此，本发明涉及在分离介质上样品应用生物分子的方法，包括以下步骤：a）通过磁珠从样品中获得所述生物分子; b）将具有生物分子的磁珠施加到分离介质; c）将生物分子释放到分离介质中，以及d）在分离介质中分离生物分子。

公开(公告)号：US20090289213A1

公开(公告)日：2009-11-26

申请号：US12279308

申请日：2006-02-13

申请人： Juergen Pipper ,  Tseng-Ming Hsieh ,  Pavel Neuzil

发明人： Juergen Pipper ,  Tseng-Ming Hsieh ,  Pavel Neuzil

IPC分类号： H01F1/00

CPC分类号： C07K1/22 ,  B01D15/3804 ,  B01J20/28009 ,  B01J2220/54 ,  B03C1/01 ,  C12M25/01 ,  G01N33/5432 ,  G01N33/5436 ,  G01N2446/00

摘要： The invention provides a method of processing a biological and/or chemical sample. The method includes providing a fluid droplet, which includes an inner phase and an outer phase. The outer phase is immiscible with the inner phase, and the outer phase is surrounding the inner phase. The inner phase includes the biological and/or chemical sample. The fluid droplet furthermore comprises magnetically attractable matter. The method also includes providing at least one surface, which is of such a texture and such a wettability for the fluid of the inner phase of the fluid droplet, that the fluid droplet remains intact upon being contacted therewith. The method further includes disposing the fluid droplet onto the at least one surface. The method also includes performing a process on the biological and/or chemical sample in the fluid droplet.

摘要翻译： 本发明提供了一种处理生物和/或化学样品的方法。 该方法包括提供包括内相和外相的流体液滴。 外相与内相不混溶，外相围绕内相。 内相包括生物和/或化学样品。 流体液滴还包括磁吸引物质。 该方法还包括提供至少一个表面，其具有这样的质地和对流体液滴的内相的流体的润湿性，使得流体液滴在与其接触时保持完整。 该方法还包括将流体液滴设置在至少一个表面上。 该方法还包括对流体液滴中的生物和/或化学样品进行处理。

公开(公告)号：US20080108151A1

公开(公告)日：2008-05-08

申请号：US11928903

申请日：2007-10-30

申请人： Roger Piasio ,  Nathan Turner

发明人： Roger Piasio ,  Nathan Turner

IPC分类号： G01N33/553

CPC分类号： G01N33/56983 ,  G01N33/54326 ,  G01N33/558 ,  G01N2446/00 ,  G01N2446/20 ,  G01N2446/80 ,  Y10S435/805 ,  Y10S435/81 ,  Y10S435/97 ,  Y10S436/806 ,  Y10T436/25375

摘要： Superparamagnetic (“SPM”) subunits of 1-30 nm average mean diameter (e.g. ferro fluid) subparticles are treated with a magnetically noninterfering substance capable of coating and covering them (e.g, BSA) and they spontaneously form agglomerates of about 100 nm to about 450 nm or higher average mean diameter and are then used to form complexes with target biological ligands such as viruses, contained in large volumes of liquid. The complexes are subjected to the gradient intensity of a strong magnetic field, and excess liquid is removed, where upon an immunochromatographic assay is conducted to determine the identity and/or amount of target ligand present, in which operation SPM particles that bonded to the ligand function as tags for ligand detection.

摘要翻译： 使用能够涂覆和覆盖它们的磁性非干扰性物质（例如BSA）处理1-30nm平均平均直径（例如铁流体）子粒子的超顺磁性（“SPM”）亚基，并且它们自发形成约100nm至约 450nm或更高的平均直径，然后用于与包含在大体积液体中的目标生物配体如病毒形成复合物。 络合物经受强磁场的梯度强度，并除去过量的液体，其中进行免疫色谱测定以确定存在的靶配体的同一性和/或量，其中与配体结合的操作SPM颗粒 作为配体检测的标签。

公开(公告)号：US07048890B2

公开(公告)日：2006-05-23

申请号：US10498958

申请日：2002-12-17

申请人： Reinder Coehoorn ,  Menno Willem Jose Prins

发明人： Reinder Coehoorn ,  Menno Willem Jose Prins

IPC分类号： G01N27/06 ,  G01N27/02 ,  G01N27/12

CPC分类号： G01R33/12 ,  B82Y25/00 ,  C12Q1/6825 ,  G01N15/0656 ,  G01N27/745 ,  G01N33/54326 ,  G01N33/5438 ,  G01N2446/00 ,  G01R33/093 ,  G01R33/096 ,  G01R33/1269 ,  H01L43/08 ,  C12Q2563/155 ,  C12Q2563/143

摘要： Method and device for magnetic detection of binding of biological molecules on a biochip in which a magnetoresistive sensor device measures an areal density of magnetic nanoparticles on a micro-array, the magnetic nanoparticles being directly or indirectly coupled to a target sample. The magnetoresistive sensor device includes a substrate having attached thereto binding sites able to selectively bind the target sample, and a magnetoresistive sensor for detecting the magnetic field of the nanoparticles coupled to the target sample. The magnetoresistive sensor includes a plurality of magnetoresistive sensing elements, the width and length dimensions of which are at least a factor 10 or more, preferably a factor 100 or more larger than the diameter of the nanoparticles.

摘要翻译： 用于磁性检测生物分子在生物芯片上的结合的方法和装置，其中磁阻传感器装置测量微阵列上的磁性纳米颗粒的面密度，磁性纳米颗粒直接或间接耦合到目标样品。 磁阻传感器装置包括附着于其上的能够选择性地结合目标样品的结合位置的基底和用于检测与目标样品相结合的纳米颗粒的磁场的磁阻传感器。 磁阻传感器包括多个磁阻感测元件，其宽度和长度尺寸至少为纳米颗粒直径的10倍或更大，优选为100倍或更大。

公开(公告)号：US07018849B2

公开(公告)日：2006-03-28

申请号：US10044920

申请日：2002-01-15

申请人： Roger N. Piasio ,  Nathan Turner

发明人： Roger N. Piasio ,  Nathan Turner

IPC分类号： G01N33/553

CPC分类号： G01N33/56983 ,  G01N33/54326 ,  G01N33/558 ,  G01N2446/00 ,  G01N2446/20 ,  G01N2446/80 ,  Y10S435/805 ,  Y10S435/81 ,  Y10S435/97 ,  Y10S436/806 ,  Y10T436/25375

摘要： Superparamagnetic (“SPM”) subunits of 1–30 nm average mean diameter (e.g. ferro fluid) subparticles are treated with a magnetically noninterfering substance capable of coating and covering them (e.g, BSA) and they spontaneously form agglomerates of about 100 nm to about 450 nm or higher average mean diameter and are then used to form complexes with target biological ligands such as viruses, contained in large volumes of liquid. The complexes are subjected to the gradient intensity of a strong magnetic field, and excess liquid is removed, where upon an immunochromatographic assay is conducted to determine the identity and/or amount of target ligand present, in which operation SPM particles that bonded to the ligand function as tags for ligand detection.

摘要翻译： 使用能够涂覆和覆盖它们的磁性非干扰性物质（例如BSA）处理1-30nm平均平均直径（例如铁流体）子粒子的超顺磁性（“SPM”）亚基，并且它们自发形成约100nm至约 450nm或更高的平均直径，然后用于与包含在大体积液体中的目标生物配体如病毒形成复合物。 络合物经受强磁场的梯度强度，并除去过量的液体，其中进行免疫色谱测定以确定存在的靶配体的同一性和/或量，其中与配体结合的操作SPM颗粒 作为配体检测的标签。

公开(公告)号：US20050032051A1

公开(公告)日：2005-02-10

申请号：US10296489

申请日：2001-06-19

申请人： Mark Hayes ,  Nolan Polson ,  Anil Vuppu ,  Timothy Crowley ,  Antonio Garcia

发明人： Mark Hayes ,  Nolan Polson ,  Anil Vuppu ,  Timothy Crowley ,  Antonio Garcia

IPC分类号： C12M1/34 ,  C12Q1/68 ,  G01N33/543

CPC分类号： G01N33/54326 ,  G01N33/54333 ,  G01N2446/00

摘要： A microimmunoassay arrangement including paramagnetic particles having labeled antibody held in a microchannel by an external magnetic field provides rapid analysis using small sample volumes.

摘要翻译： 包含具有通过外部磁场保持在微通道中的标记抗体的顺磁性颗粒的微量免疫测定装置提供使用小样品体积的快速分析。

公开(公告)号：US20040018611A1

公开(公告)日：2004-01-29

申请号：US10202462

申请日：2002-07-23

发明人： Michael Dennis Ward ,  Piotr Grodzinski ,  Robin Hui Liu ,  Bradley Neal Engel ,  Yingjie Liu

IPC分类号： C12M001/34 ,  G01N033/553

CPC分类号： B82Y30/00 ,  B01L3/502746 ,  B01L3/502761 ,  B01L7/52 ,  B01L2200/0668 ,  B01L2200/10 ,  B01L2300/0816 ,  B01L2400/0415 ,  B01L2400/043 ,  B01L2400/0487 ,  B01L2400/086 ,  B82Y15/00 ,  G01N33/54326 ,  G01N35/0098 ,  G01N2446/00

摘要： The present invention provides microfluidic devices that can be used to effect a number of manipulations on a sample to ultimately result in target analyte detection or quantification. The device provides at least one magnetic microchannel that is capable of separating magnetic or magnetically-labeled target analytes from non-magnetic materials. Further, a magnetic microchannel may sort materials according to their magnetic response. Alternatively, magnetic or magnetically-labeled components other than the target analytes can be retained by the magnetic microchannel and are thus removed from the target analytes. Depending on the specificity of the binding ligand, one can either separate a vast population of analytes sharing a common binding motif, or specifically retain a rare target analyte because of its recognition of a specific ligand on the magnetic particle.

摘要翻译： 本发明提供了微流体装置，其可用于对样品进行多次操作以最终导致目标分析物检测或定量。 该装置提供至少一个能够从非磁性材料分离磁性或磁性标记的目标分析物的磁性微通道。 此外，磁性微通道可根据其磁响应分类材料。 或者，除了目标分析物之外的磁性或磁性标记的组分可被磁性微通道保留，因此从目标分析物中除去。 根据结合配体的特异性，可以分离共享共同结合基序的大量分析物，或者由于其识别了磁性颗粒上的特定配体，特异性地保留了罕见的目标分析物。

公开(公告)号：US20030134434A1

公开(公告)日：2003-07-17

申请号：US10044920

申请日：2002-01-15

发明人： Roger N. Piasio ,  Nathan Turner

IPC分类号： G01N033/558 ,  G01N033/553

CPC分类号： G01N33/56983 ,  G01N33/54326 ,  G01N33/558 ,  G01N2446/00 ,  G01N2446/20 ,  G01N2446/80 ,  Y10S435/805 ,  Y10S435/81 ,  Y10S435/97 ,  Y10S436/806 ,  Y10T436/25375

摘要： Superparamagnetic (nullSPMnull) subunits of 1-30 nm average mean diameter (e.g. ferro fluid) subparticles are treated with a magnetically noninterfering substance capable of coating and covering them (e.g, BSA) and they spontaneously form agglomerates of about 100 nm to about 450 nm or higher average mean diameter and are then used to form complexes with target biological ligands such as viruses, contained in large volumes of liquid. The complexes are subjected to the gradient intensity of a strong magnetic field, and excess liquid is removed, where upon an immunochromatographic assay is conducted to determine the identity and/or amount of target ligand present, in which operation SPM particles that bonded to the ligand function as tags for ligand detection.

摘要翻译： 使用能够涂覆和覆盖它们的磁性非干扰性物质（例如BSA）处理1-30nm平均平均直径（例如铁流体）子粒子的超顺磁性（“SPM”）亚基，并且它们自发形成约100nm至约 450nm或更高的平均直径，然后用于与包含在大体积液体中的目标生物配体如病毒形成复合物。 络合物经受强磁场的梯度强度，并除去过量的液体，其中进行免疫色谱测定以确定存在的靶配体的同一性和/或量，其中与配体结合的操作SPM颗粒 作为配体检测的标签。

公开(公告)号：US09733315B2

公开(公告)日：2017-08-15

申请号：US13762622

申请日：2013-02-08

申请人： University of Houston

发明人： Dmitri Litvinov ,  Richard Willson

IPC分类号： G01R33/00 ,  G01R33/12

CPC分类号： G01R33/00 ,  G01N2446/00 ,  G01R33/1269

摘要： A biomolecular sensor system includes an array of magnetoresistive nanosensors designed for sensing biomolecule-conjugated superparamagnetic nanoparticles. Materials and geometry of each sensor element are designed for optimized sensitivity. The system includes magnetic field generators to apply forces to superparamagnetic nanoparticles for 1) nanoparticle manipulation, 2) sensor magnetic biasing, 3) magnetic pull-off measurement for differentiation against non-specific association, and 4) removal of all particles from the sensor array surface.

公开(公告)号：US20170016922A1

公开(公告)日：2017-01-19

申请号：US15330443

申请日：2016-09-22

申请人： Henry A. Graham ,  John G. Gorman ,  James P. Rowell

发明人： Henry A. Graham ,  John G. Gorman ,  James P. Rowell

IPC分类号： G01N33/80

CPC分类号： G01N33/80 ,  G01N2446/00

摘要： Methods for separating, in a continuous, multizone fluid medium, cells, particles, or other molecules of interest (MOI) from associated or contaminating unwanted materials not of interest (MNOI). The invention involves forced movement of MOI into certain zones having properties which deter the entry of unwanted materials. Differential movement of MOI and MNOI occurs by active counterforces that move MNOI but not MOI. MOI are tagged with magnetic particles and moved with a magnetic field through a fluid, or zones, of higher specific gravity that prevents, by flotation counterforce, unwanted less dense materials from entering.Surfaces specifically coated with reactants are reactive with the MOI in the tagged magnetic particle complex and of buoyant or other forces are used to remove any unbound material from the surface before reading. Readable labels, in addition to the magnetic particle tagged complex itself, such as enzymes, fluorophors, chemiluminescent materials, radioactive isotopes, chromogenic and fluorogenic substrates and other labels may be used. In most embodiments, materials of interest are delivered to a special final zone for reading or harvesting. The invention applies to many assays, diagnostic tests, separative procedures and chemical syntheses.

摘要翻译： 特别涂覆有反应物的表面与标记的磁性颗粒复合体中的MOI反应，并且在读取之前使用浮力或其它力从表面去除任何未结合的材料。 可以使用除了磁性颗粒标记的复合体本身之外的可读标签，例如酶，荧光体，化学发光材料，放射性同位素，显色和荧光底物等标记。 在大多数实施例中，感兴趣的材料被输送到用于阅读或收获的特殊最后区域。 本发明适用于许多测定，诊断测试，分离程序和化学合成。