公开(公告)号：US11535863B2

公开(公告)日：2022-12-27

申请号：US16439840

申请日：2019-06-13

申请人： President and Fellows of Harvard College

发明人： George M. Church ,  Prashant G. Mali ,  Luhan Yang

IPC分类号： C12N15/00 ,  C12N15/85 ,  C12N15/10 ,  C12N15/63 ,  C12N15/90 ,  C12N15/01 ,  C12N15/81 ,  C12N15/82 ,  C12N15/87 ,  C12N9/22

摘要： A method of altering a eukaryotic cell is provided including transfecting the eukaryotic cell with a nucleic acid encoding RNA complementary to genomic DNA of the eukaryotic cell, transfecting the eukaryotic cell with a nucleic acid encoding an enzyme that interacts with the RNA and cleaves the genomic DNA in a site specific manner, wherein the cell expresses the RNA and the enzyme, the RNA binds to complementary genomic DNA and the enzyme cleaves the genomic DNA in a site specific manner.

公开(公告)号：US11499137B2

公开(公告)日：2022-11-15

申请号：US16143112

申请日：2018-09-26

申请人： Wisconsin Alumni Research Foundation

发明人： Igor Slukvin ,  Irina Elcheva

IPC分类号： C12N5/00 ,  C12N5/0735 ,  C12N5/0789 ,  C12N15/00 ,  C12N15/11 ,  C12N15/66 ,  C12N5/071

摘要： Described herein are methods and related compositions for inducing differentiation of human pluripotent stem cells (hPSCs) into hemogenic endothelium with pan-myeloid potential or restricted potential, by forced expression in the hPSCs of a combination of transcription factors as described herein.

公开(公告)号：US11466071B2

公开(公告)日：2022-10-11

申请号：US16769144

申请日：2018-12-03

申请人： The United States of America, as represented by the Secretary, Department of Health and Human Services

发明人： Rami Yoseph ,  Yong-Chen Lu ,  Gal Cafri ,  Steven A. Rosenberg

IPC分类号： C07K14/725 ,  G01N33/574 ,  A61K38/00 ,  C12N15/00

摘要： Disclosed is an isolated or purified T cell receptor (TCR), wherein the TCR has antigenic specificity for a mutated RAS amino acid sequence presented by a human leukocyte antigen (HLA) Class I molecule. Related polypeptides and proteins, as well as related nucleic acids, recombinant expression vectors, host cells, populations of cells, and pharmaceutical compositions are also provided. Also disclosed are methods of detecting the presence of cancer in a mammal and methods of treating or preventing cancer in a mammal.

公开(公告)号：US11459585B2

公开(公告)日：2022-10-04

申请号：US14903719

申请日：2014-07-08

申请人： President and Fellows of Harvard College

发明人： George M. Church ,  James Dicarlo

IPC分类号： C12N15/00 ,  C12N15/90 ,  C12N15/11

摘要： Methods of multiplex genome engineering in cells using Cas9 is provided which includes a cycle of steps of introducing into the cell a first foreign nucleic acid encoding one or more RNAs complementary to the target DNA and which guide the enzyme to the target DNA, wherein the one or more RNAs and the enzyme are members of a co-localization complex for the target DNA, and introducing into the cell a second foreign nucleic acid encoding one or more donor nucleic acid sequences, and wherein the cycle is repeated a desired number of times to multiplex DNA engineering in cells.

公开(公告)号：US11453891B2

公开(公告)日：2022-09-27

申请号：US15975728

申请日：2018-05-09

申请人： The Regents of the University of California

发明人： Eugene Yeo ,  Kristopher Brannan ,  Ryan Marina ,  David Nelles

IPC分类号： C12N15/00 ,  C12N15/85 ,  C12N15/11 ,  A61P21/00 ,  C12N9/22 ,  C12N15/113 ,  A61K48/00

摘要： Disclosed herein is a technology to perform programmable RNA editing at single-nucleotide resolution using RNA-targeting CRISPR/Cas9. This approach, which Applicants have termed “Cas9-directed RNA editing” or “CREDIT,” provides a means to reversibly alter genetic information in a temporal manner, unlike traditional CRISPR/Cas9 driven genomic engineering which relies on permanently altering DNA sequence.

公开(公告)号：US11441164B2

公开(公告)日：2022-09-13

申请号：US17099539

申请日：2020-11-16

申请人： CB Therapeutics, INC.

发明人： Jacob Michael Vogan ,  Laura Flatauer Peiffer ,  James Lee Wade ,  Tyrone Jacob Yacoub ,  Kirsten Tang ,  Rachel Nadine Burnett

IPC分类号： C12P17/10 ,  C12N9/88 ,  C12N9/02 ,  C12N9/12 ,  C12N9/10 ,  C12N1/20 ,  C12N15/00 ,  C12N1/16 ,  C12N15/81

摘要： The systems and methods herein include engineering a host to produce psilocybin using engineered enzymes, genetic changes, and exogenous psilocybin precursor addition (e.g., addition of L-tryptophan to a growing culture of a psilocybin producing recombinant host strain). The process occurs in genetically engineered host cell(s) that can produce psilocybin.

公开(公告)号：US11427829B2

公开(公告)日：2022-08-30

申请号：US15536836

申请日：2015-12-15

申请人： Danisco US Inc.

发明人： Benjamin S. Bower ,  Jimmy Chan ,  Jing Ge ,  Xiaogang Gu ,  Susan Mampusti Madrid ,  Danfeng Song ,  Mingmin Song ,  Michael Ward

IPC分类号： C12N15/00 ,  C12N15/80 ,  C12N9/22 ,  C12N15/11

摘要： Compositions and methods are provided for genome modification at a target site in the genome of a filamentous fungal cell. The methods and compositions are drawn to a guide polynucleotide/Cas endonuclease system for modifying or altering the target site. Aspects in which the filamentous fungal cell being modified has a defective non-homologous end joining pathway are also provided.

公开(公告)号：US11421022B2

公开(公告)日：2022-08-23

申请号：US14579192

申请日：2014-12-22

申请人： Hoffmann-La Roche Inc.

发明人： Sebastian Fenn ,  Erhard Kopetzki

IPC分类号： C07H21/04 ,  C12P21/06 ,  C12N15/00 ,  C12N5/00 ,  C07K16/00 ,  C07K16/18 ,  C07K16/32 ,  C12N15/62 ,  C12N9/52 ,  A61K47/68

摘要： Herein is reported a method for producing an antibody Fc-region conjugate comprising as first component an antibody Fc-region and as second component at least one binding entity that specifically binds to a target using a transpeptidase for enzymatic conjugation of the antibody Fc-region to at least one binding entity.

公开(公告)号：US20220220438A1

公开(公告)日：2022-07-14

申请号：US17651505

申请日：2022-02-17

申请人： University of Massachusetts

发明人： Pablo E. Visconti ,  Felipe Navarrete

IPC分类号： C12N5/073 ,  A01N1/02 ,  A61K48/00 ,  C12N5/076 ,  C12N15/00 ,  A61B17/43 ,  A61B17/435 ,  A61D19/02 ,  A61D19/04

摘要： Methods for improving the functionality and/or fertility of sperm, for example, by enhancing motility and/or extending the lifespan of sperm by subjecting the isolated sperm to a starvation protocol and/or ionophore are provided. Such methods may be used in, for example, artificial insemination to reduce the number of sperm needed for insemination and to improve conception rates.

公开(公告)号：US11384134B2

公开(公告)日：2022-07-12

申请号：US16857130

申请日：2020-04-23

申请人： Emory University

发明人： Brantley R. Herrin ,  Max Dale Cooper ,  Rudolf Ehrhardt

IPC分类号： C07K14/46 ,  C12N15/00 ,  C07K14/725

摘要： This disclosure relates to variable lymphocyte receptors (VLRs) modifications such as humanized sequences and polypeptides comprising such sequences that specifically bind a target molecule and uses related thereto. In certain embodiments, the disclosure relates to recombinant polypeptide VLRs disclosed herein and variants thereof. In certain embodiments, this disclosure relates to treating or preventing a disease or condition comprising administering an effective amount of a recombinant polypeptide or variant disclosed herein to a subject in need thereof.