公开(公告)号：US5846802A

公开(公告)日：1998-12-08

申请号：US225488

申请日：1994-04-08

申请人： Frank Buxton ,  Albert Hinnen ,  Jacob Visser

发明人： Frank Buxton ,  Albert Hinnen ,  Jacob Visser

IPC分类号： C12N9/62 ,  C12N15/57 ,  C12N15/80 ,  C12N9/12 ,  C12P19/34

CPC分类号： C12N15/80 ,  C12N9/62

摘要： The present invention concerns a novel DNA sequence coding for an Aspergillus serine protease of the subtilisin-type, an Aspergillus serine protease of the subtilisin-type per se and a method for the preparation thereof. The invention further concerns a novel Aspergillus mutant strain defective in a serine protease of the subtilisin-type, which is useful for the expression of heterologous protein, and a method for the preparation of such a mutant strain.

摘要翻译： 本发明涉及编码枯草杆菌蛋白酶类型的曲霉丝氨酸蛋白酶的新型DNA序列，枯草杆菌蛋白酶型本身的曲霉丝氨酸蛋白酶及其制备方法。 本发明还涉及可用于表达异源蛋白质的枯草杆菌蛋白酶型丝氨酸蛋白酶中缺陷的新型曲霉菌突变菌株，以及这种突变菌株的制备方法。

公开(公告)号：US5693510A

公开(公告)日：1997-12-02

申请号：US608452

申请日：1996-02-28

申请人： Debbie Sue Yaver ,  Sheryl Ann Thompson

发明人： Debbie Sue Yaver ,  Sheryl Ann Thompson

IPC分类号： C12N15/09 ,  C12N1/14 ,  C12N1/15 ,  C12N9/48 ,  C12N9/62 ,  C12N15/57 ,  C12N15/81 ,  C12P21/02 ,  C12N15/00 ,  C12N1/16 ,  C12N1/18

CPC分类号： C12N9/48 ,  C12N15/815

摘要： The present invention relates to a gene encoding an ascomycete or deuteromycete carboxypeptidase Y gene, and host cells modified so as to produce reduced amounts of carboxypeptidase.

摘要翻译： 本发明涉及编码子囊菌或十二生菌羧肽酶Y基因的基因，以及修饰的宿主细胞以产生较少量的羧肽酶。

公开(公告)号：US5674728A

公开(公告)日：1997-10-07

申请号：US328314

申请日：1994-10-24

申请人： Frank Buxton ,  Gabor Jarai ,  Jacob Visser

发明人： Frank Buxton ,  Gabor Jarai ,  Jacob Visser

IPC分类号： C12N15/09 ,  C12N1/15 ,  C12N9/62 ,  C12N15/00 ,  C12N15/57 ,  C12N15/58 ,  C12R1/685 ,  C12N15/80 ,  C12N15/90

CPC分类号： C12N9/62

摘要： The present invention concerns a novel DNA sequence coding for an Aspergillus aspartic protease, an Aspergillus aspartic protease per se and a method for the preparation thereof. The invention further concerns a novel Aspergillus mutant strain defective in a protease of the aspartic proteinase-type, which is useful for the expression of heterologous protein, and a method for the preparation of such a mutant strain.

摘要翻译： 本发明涉及编码曲霉天冬氨酸蛋白酶，曲霉天门冬氨酸蛋白酶本身的新型DNA序列及其制备方法。 本发明还涉及可用于异源蛋白质表达的天冬氨酸蛋白酶型蛋白酶中缺陷的新型曲霉菌突变株，以及这种突变菌株的制备方法。

公开(公告)号：US5445957A

公开(公告)日：1995-08-29

申请号：US352703

申请日：1994-12-05

申请人： Rodger R. Rohde, Jr. ,  Edward F. Schuler ,  Richard A. Handel

发明人： Rodger R. Rohde, Jr. ,  Edward F. Schuler ,  Richard A. Handel

IPC分类号： A61K38/54 ,  C12N9/24 ,  C12N9/20 ,  C12N9/42 ,  C12N9/62

CPC分类号： A61K38/47 ,  C12Y302/01004 ,  C12Y302/01026

摘要： This invention relates to a beta-fructofuranosidase enzyme food supplement composition, which alleviates gastrointestinal distress caused by ingested food containing oligosaccharides, comprising a beta-fructofuranosidase enzyme, a cellulase enzyme and a hemicellulase enzyme. More particularly, the enzyme food supplement composition of this invention comprises a beta-fructofuranosidase enzyme, a cellulase enzyme, a hemicellulase enzyme, a lipase enzyme and an acid protease enzyme. This invention also relates to a method of alleviating gastrointestinal distress in the gastrointestinal system of a human being, which distress is caused by ingested food containing oligosaccharides, the method comprising the step of ingesting a beta-fructofuranosidase enzyme food supplement composition comprising a beta-fructofuranosidase enzyme, a cellulase enzyme and a hemicellulase enzyme, to convert oligosaccharides contained in the ingested food to reducing sugars. More particularly, the method of alleviating gastrointestinal distress of this invention comprises the step of ingesting an enzyme food supplement composition comprising a beta-fructofuranosidase enzyme, a cellulase enzyme, a hemicellulase enzyme, a lipase enzyme and an acid protease enzyme, to convert oligosaccharides contained in the ingested food to reducing sugars.

摘要翻译： 本发明涉及一种β-呋喃果糖苷酶食品补充剂组合物，该组合物可减轻由含有β-呋喃果糖苷酶，纤维素酶和半纤维素酶的含有低聚糖的食物引起的胃肠道窘迫。 更具体地，本发明的酶食品补充剂组合物包含β-呋喃果糖苷酶，纤维素酶，半纤维素酶，脂肪酶和酸性蛋白酶。 本发明还涉及一种减轻胃肠道系统损伤的方法，所述方法包括以下步骤：摄入含有β-呋喃果糖苷酶的β-呋喃果糖苷酶食品补充剂组合物，所述方法包括： 酶，纤维素酶和半纤维素酶，以将摄取的食物中含有的寡糖转化为还原糖。 更具体地，本发明的减轻胃肠道窘迫的方法包括摄取包含β-呋喃果糖苷酶，纤维素酶，半纤维素酶，脂肪酶和酸性蛋白酶的酶食品补充剂组合物以转化所含的寡糖的步骤 在摄入的食物中减少糖。

公开(公告)号：US4532213A

公开(公告)日：1985-07-30

申请号：US559341

申请日：1983-12-08

申请人： Jayarama K. Shetty ,  James J. Marshall

发明人： Jayarama K. Shetty ,  James J. Marshall

IPC分类号： C12N9/34 ,  C12N9/62 ,  C12N9/58

CPC分类号： C12N9/2428 ,  C12N9/62 ,  Y10S435/814

摘要： Glucoamylase and acid fungal protease co-produced by the fermentation of fungal species capable of producing these enzymes are separated by carrying out the fermentation at a pH at which the protease associates itself with the fungal mycelium and removing the mycelium from the fermentation broth. The mycelium is then resuspended in an aqueous medium and the pH adjusted to cause the dissociation of the protease and mycelium with subsequent recovery of the protease from the aqueous medium.

摘要翻译： 通过发酵能够产生这些酶的真菌物质共同产生的葡糖淀粉酶和酸性真菌蛋白酶通过在蛋白酶与真菌菌丝体相关联并从发酵液中除去菌丝体的pH进行发酵来分离发酵。 然后将菌丝体重新悬浮在水性介质中，并调节pH以引起蛋白酶和菌丝体的解离，随后从水性介质中回收蛋白酶。

公开(公告)号：US3281331A

公开(公告)日：1966-10-25

申请号：US23186762

申请日：1962-10-19

申请人： ASTRA AB

发明人： ROLF BERGKVIST

IPC分类号： C12N9/58 ,  C12N9/62

CPC分类号： C12N9/58 ,  C12N9/62 ,  Y10S424/13 ,  Y10S435/815 ,  Y10S435/918

公开(公告)号：US20240271167A1

公开(公告)日：2024-08-15

申请号：US18637805

申请日：2024-04-17

申请人： Novozymes A/S

发明人： Randall Scott DEINHAMMER ,  Joyce CRAIG ,  Suzanne CLARK ,  John MATTHEWS ,  Anne Glud HJULMAND ,  Chee-Leong SOONG ,  Zhengfang KANG

IPC分类号： C12P7/14 ,  C12N9/26 ,  C12N9/28 ,  C12N9/44 ,  C12N9/52 ,  C12N9/58 ,  C12N9/62 ,  C12P7/06 ,  C12P7/08 ,  C12P19/14 ,  C13K1/06

CPC分类号： C12P7/14 ,  C12N9/2414 ,  C12N9/2417 ,  C12N9/2457 ,  C12N9/52 ,  C12N9/58 ,  C12N9/62 ,  C12P7/06 ,  C12P7/08 ,  C12P19/14 ,  C13K1/06 ,  C12P2201/00 ,  C12P2203/00 ,  Y02E50/10

摘要： The present invention relates to processes for producing fermentation products from starch-containing material, wherein an alpha-amylase and optionally a thermostable protease, pullulanase and/or glucoamylase are present and/or added during liquefaction, wherein a cellulolytic composition is present and/or added during fermentation or simultaneous saccharification and fermentation. The invention also relates to a composition suitable for use in a process of the invention.

公开(公告)号：US11427812B2

公开(公告)日：2022-08-30

申请号：US16461816

申请日：2017-11-20

申请人： LYSANDO AG

发明人： Manfred Biebl ,  Martin Griessl ,  Kristin Neumann

IPC分类号： C12N9/24 ,  A61P31/04 ,  A61L2/16 ,  C12N9/10 ,  C12N9/52 ,  C12N9/62 ,  A61K38/08 ,  A61K38/00 ,  C12N9/36 ,  C12N9/64

摘要： The present invention relates to the field of antimicrobial agents active against Staphylococcus aureus bacteria. In particular, the present invention relates to polypeptides comprising the CHAP domain of LysK endolysin, the M23 endopeptidase domain of lysostaphin, the cell wall binding domain (CBD) of ALE-1, and a further peptide selected from the group consisting of an antimicrobial peptide, an amphipathic peptide, a cationic peptide, a hydrophobic peptide, a sushi peptide and a defensin. In addition, the present invention relates to nucleic acids encoding such polypeptides, vectors comprising such nucleic acids, and corresponding host cells, compositions and devices. Finally, the present invention relates to applications of the inventive polypeptides, in particular in the pharmaceutical field.

公开(公告)号：US20220023396A1

公开(公告)日：2022-01-27

申请号：US17494733

申请日：2021-10-05

申请人： AMANO ENZYME INC. ,  HIROSHIMA UNIVERSITY

发明人： Manabu KURODA ,  Shotaro YAMAGUCHI ,  Norihisa KATO

IPC分类号： A61K38/46 ,  A23L2/52 ,  A61K36/062 ,  C12N9/50 ,  A23L33/10 ,  C12N9/62 ,  A23L29/00 ,  A61K38/48 ,  A61P1/14 ,  A23L33/17 ,  A23K50/30 ,  A61P1/10 ,  A61K9/00 ,  C12N9/26 ,  C12N9/24

摘要： A method for improving intestinal flora increases the number of beneficial bacteria such as lactic acid bacteria and Bifidobacterium to improve intestinal flora by using an enzyme. The method involves providing a protease, which is a polypeptide having an amino acid sequence shown in SEQ ID NO: 1 that can increase beneficial bacteria such as lactic acid bacteria and Bifidobacterium in intestines to exert an excellent effect of improving intestinal flora.

公开(公告)号：US11015175B2

公开(公告)日：2021-05-25

申请号：US14441493

申请日：2013-11-08

申请人： TOHOKU UNIVERSITY

发明人： Keietsu Abe ,  Katsuya Gomi ,  Kei Yoshimi

IPC分类号： C12N9/10 ,  C12N15/01 ,  C12N1/15 ,  C12N9/02 ,  C12N1/14 ,  C12N15/80 ,  C12P1/02 ,  C12P7/44 ,  C12P21/02 ,  C12N9/62 ,  C12N9/16 ,  C12N9/20 ,  C12N9/26 ,  C12N9/42 ,  C12N9/48 ,  C12P7/46 ,  C12P7/48 ,  C12P17/06 ,  C12P35/00 ,  C12P37/00 ,  A61K38/00

摘要： An object to be achieved by the present invention is to culture a filamentous fungus at a high density, thereby enabling mass production of a useful substance. The present invention provides a method of producing a substance, including the steps of: culturing a mutant filamentous fungus with no expression of α-1,3-glucan to allow the filamentous fungus to produce a substance; and collecting the resulting substance.