公开(公告)号：US20100036220A1

公开(公告)日：2010-02-11

申请号：US12569506

申请日：2009-09-29

申请人： Benjamin J. Feldman ,  Adam Heller ,  Ephraim Heller ,  Fei Mao ,  Joseph A. Vivolo ,  Jeffery V. Funderburk ,  Fredric C. Colman ,  Rajesh Krishnan

发明人： Benjamin J. Feldman ,  Adam Heller ,  Ephraim Heller ,  Fei Mao ,  Joseph A. Vivolo ,  Jeffery V. Funderburk ,  Fredric C. Colman ,  Rajesh Krishnan

IPC分类号： A61B5/157

CPC分类号： G01N27/3272 ,  C12Q1/001 ,  C12Q1/006 ,  C12Q1/32 ,  G01N27/3271 ,  G01N27/3274 ,  Y10T29/49002 ,  Y10T29/49004 ,  Y10T29/49007 ,  Y10T29/49126 ,  Y10T29/49128 ,  Y10T29/4913 ,  Y10T29/49147 ,  Y10T29/49155 ,  Y10T29/49204 ,  Y10T29/49208 ,  Y10T29/4921 ,  Y10T29/49794 ,  Y10T156/1052

摘要： A sensor utilizing a non-leachable or diffusible redox mediator is described. The sensor includes a sample chamber to hold a sample in electrolytic contact with a working electrode, and in at least some instances, the sensor also contains a non-leachable or a diffusible second electron transfer agent. The sensor and/or the methods used produce a sensor signal in response to the analyte that can be distinguished from a background signal caused by the mediator. The invention can be used to determine the concentration of a biomolecule, such as glucose or lactate, in a biological fluid, such as blood or serum, using techniques such as coulometry, amperometry, and potentiometry. An enzyme capable of catalyzing the electrooxidation or electroreduction of the biomolecule is typically provided as a second electron transfer agent.

公开(公告)号：US20100019407A1

公开(公告)日：2010-01-28

申请号：US12569179

申请日：2009-09-29

申请人： Benjamin J. Feldman ,  Adam Heller ,  Ephraim Heller ,  Fei Mao ,  Joseph A. Vivolo ,  Jeffery V. Funderburk ,  Fredric C. Colman ,  Rajesh Krishnan

发明人： Benjamin J. Feldman ,  Adam Heller ,  Ephraim Heller ,  Fei Mao ,  Joseph A. Vivolo ,  Jeffery V. Funderburk ,  Fredric C. Colman ,  Rajesh Krishnan

IPC分类号： B29C53/02 ,  B05D5/12

CPC分类号： G01N27/3272 ,  C12Q1/001 ,  C12Q1/006 ,  C12Q1/32 ,  G01N27/3271 ,  G01N27/3274 ,  Y10T29/49002 ,  Y10T29/49004 ,  Y10T29/49007 ,  Y10T29/49126 ,  Y10T29/49128 ,  Y10T29/4913 ,  Y10T29/49147 ,  Y10T29/49155 ,  Y10T29/49204 ,  Y10T29/49208 ,  Y10T29/4921 ,  Y10T29/49794 ,  Y10T156/1052

摘要： A sensor utilizing a non-leachable or diffusible redox mediator is described. The sensor includes a sample chamber to hold a sample in electrolytic contact with a working electrode, and in at least some instances, the sensor also contains a non-leachable or a diffusible second electron transfer agent. The sensor and/or the methods used produce a sensor signal in response to the analyte that can be distinguished from a background signal caused by the mediator. The invention can be used to determine the concentration of a biomolecule, such as glucose or lactate, in a biological fluid, such as blood or serum, using techniques such as coulometry, amperometry, and potentiometry. An enzyme capable of catalyzing the electrooxidation or electroreduction of the biomolecule is typically provided as a second electron transfer agent.

公开(公告)号：US20100018870A1

公开(公告)日：2010-01-28

申请号：US12568936

申请日：2009-09-29

申请人： Benjamin J. Feldman ,  Adam Heller ,  Ephraim Heller ,  Fei Mao ,  Joseph A. Vivolo ,  Jeffery V. Funderburk ,  Fredric C. Colman ,  Rajesh Krishnan

发明人： Benjamin J. Feldman ,  Adam Heller ,  Ephraim Heller ,  Fei Mao ,  Joseph A. Vivolo ,  Jeffery V. Funderburk ,  Fredric C. Colman ,  Rajesh Krishnan

IPC分类号： C12Q1/26 ,  G01N27/26 ,  B32B37/00

CPC分类号： G01N27/3272 ,  C12Q1/001 ,  C12Q1/006 ,  C12Q1/32 ,  G01N27/3271 ,  G01N27/3274 ,  Y10T29/49002 ,  Y10T29/49004 ,  Y10T29/49007 ,  Y10T29/49126 ,  Y10T29/49128 ,  Y10T29/4913 ,  Y10T29/49147 ,  Y10T29/49155 ,  Y10T29/49204 ,  Y10T29/49208 ,  Y10T29/4921 ,  Y10T29/49794 ,  Y10T156/1052

摘要： A sensor utilizing a non-leachable or diffusible redox mediator is described. The sensor includes a sample chamber to hold a sample in electrolytic contact with a working electrode, and in at least some instances, the sensor also contains a non-leachable or a diffusible second electron transfer agent. The sensor and/or the methods used produce a sensor signal in response to the analyte that can be distinguished from a background signal caused by the mediator. The invention can be used to determine the concentration of a biomolecule, such as glucose or lactate, in a biological fluid, such as blood or serum, using techniques such as coulometry, amperometry, and potentiometry. An enzyme capable of catalyzing the electrooxidation or electroreduction of the biomolecule is typically provided as a second electron transfer agent.

公开(公告)号：US20100012516A1

公开(公告)日：2010-01-21

申请号：US12568864

申请日：2009-09-29

申请人： Benjamin J. Feldman ,  Adam Heller ,  Ephraim Heller ,  Fei Mao ,  Joseph A. Vivolo ,  Jeffery V. Funderburk ,  Fredric C. Colman ,  Rajesh Krishnan

发明人： Benjamin J. Feldman ,  Adam Heller ,  Ephraim Heller ,  Fei Mao ,  Joseph A. Vivolo ,  Jeffery V. Funderburk ,  Fredric C. Colman ,  Rajesh Krishnan

IPC分类号： C12Q1/00 ,  G01N27/26 ,  B32B38/10

CPC分类号： G01N27/3272 ,  C12Q1/001 ,  C12Q1/006 ,  C12Q1/32 ,  G01N27/3271 ,  G01N27/3274 ,  Y10T29/49002 ,  Y10T29/49004 ,  Y10T29/49007 ,  Y10T29/49126 ,  Y10T29/49128 ,  Y10T29/4913 ,  Y10T29/49147 ,  Y10T29/49155 ,  Y10T29/49204 ,  Y10T29/49208 ,  Y10T29/4921 ,  Y10T29/49794 ,  Y10T156/1052

摘要： A sensor utilizing a non-leachable or diffusible redox mediator is described. The sensor includes a sample chamber to hold a sample in electrolytic contact with a working electrode, and in at least some instances, the sensor also contains a non-leachable or a diffusible second electron transfer agent. The sensor and/or the methods used produce a sensor signal in response to the analyte that can be distinguished from a background signal caused by the mediator. The invention can be used to determine the concentration of a biomolecule, such as glucose or lactate, in a biological fluid, such as blood or serum, using techniques such as coulometry, amperometry, and potentiometry. An enzyme capable of catalyzing the electrooxidation or electroreduction of the biomolecule is typically provided as a second electron transfer agent.

公开(公告)号：US20100012492A1

公开(公告)日：2010-01-21

申请号：US12569311

申请日：2009-09-29

申请人： Benjamin J. Feldman ,  Adam Heller ,  Ephraim Heller ,  Fei Mao ,  Joseph A. Vivolo ,  Jeffery V. Funderburk ,  Fredric C. Colman ,  Rajesh Krishnan

发明人： Benjamin J. Feldman ,  Adam Heller ,  Ephraim Heller ,  Fei Mao ,  Joseph A. Vivolo ,  Jeffery V. Funderburk ,  Fredric C. Colman ,  Rajesh Krishnan

IPC分类号： G01N27/26

CPC分类号： G01N27/3272 ,  C12Q1/001 ,  C12Q1/006 ,  C12Q1/32 ,  G01N27/3271 ,  G01N27/3274 ,  Y10T29/49002 ,  Y10T29/49004 ,  Y10T29/49007 ,  Y10T29/49126 ,  Y10T29/49128 ,  Y10T29/4913 ,  Y10T29/49147 ,  Y10T29/49155 ,  Y10T29/49204 ,  Y10T29/49208 ,  Y10T29/4921 ,  Y10T29/49794 ,  Y10T156/1052

摘要： A sensor utilizing a non-leachable or diffusible redox mediator is described. The sensor includes a sample chamber to hold a sample in electrolytic contact with a working electrode, and in at least some instances, the sensor also contains a non-leachable or a diffusible second electron transfer agent. The sensor and/or the methods used produce a sensor signal in response to the analyte that can be distinguished from a background signal caused by the mediator. The invention can be used to determine the concentration of a biomolecule, such as glucose or lactate, in a biological fluid, such as blood or serum, using techniques such as coulometry, amperometry, and potentiometry. An enzyme capable of catalyzing the electrooxidation or electroreduction of the biomolecule is typically provided as a second electron transfer agent.

公开(公告)号：US20090305410A1

公开(公告)日：2009-12-10

申请号：US12334387

申请日：2008-12-12

申请人： Fei Mao ,  Wai-Yee Leung ,  Ching-Ying Cheung ,  Hye Eun Hoover

发明人： Fei Mao ,  Wai-Yee Leung ,  Ching-Ying Cheung ,  Hye Eun Hoover

IPC分类号： C12N5/06 ,  C07D209/20 ,  C07D403/06 ,  C07D413/14 ,  C07H17/02 ,  C07K2/00 ,  C07D311/08 ,  C07D405/12 ,  C07K16/00 ,  C07H21/00 ,  C07D491/147

CPC分类号： G01N33/582 ,  C09B11/08 ,  C09B11/24 ,  C09B23/0066 ,  C09B23/06 ,  C09B23/083 ,  C09B23/086 ,  C09B57/001 ,  C09B57/02 ,  C09B69/00 ,  G01N21/6428 ,  G01N2021/6439

摘要： The present invention relates to fluorescent dyes in general. The present invention provides a wide range of fluorescent dyes and kits containing the same, which are applicable for labeling a variety of biomolecules, cells and microorganisms. The present invention also provides various methods of using the fluorescent dyes for research and development, forensic identification, environmental studies, diagnosis, prognosis, and/or treatment of disease conditions.

摘要翻译： 本发明一般涉及荧光染料。 本发明提供了广泛的荧光染料和含有该荧光染料的试剂盒，其可用于标记各种生物分子，细胞和微生物。 本发明还提供使用荧光染料进行研究，开发鉴定，环境研究，诊断，预后和/或治疗疾病的各种方法。

公开(公告)号：US07601498B2

公开(公告)日：2009-10-13

申请号：US11377254

申请日：2006-03-16

申请人： Fei Mao ,  Wai-Yee Leung

发明人： Fei Mao ,  Wai-Yee Leung

IPC分类号： C12Q1/68 ,  C07H21/00 ,  C07H21/02 ,  C12Q1/00 ,  C12Q1/70

CPC分类号： C09B15/00 ,  C09B11/28 ,  C09B23/04 ,  C12Q1/6818 ,  G01N1/30 ,  G01N21/6486 ,  Y10S435/81 ,  Y10T436/143333 ,  C12Q2525/301

摘要： Methods of using dyes and associated technology are provided. A dye, such as a monomeric dye or a dimeric dye, may be used in a nucleic acid gel staining application and/or a nucleic acid detection application. Such a dye and a salt that comprises an anion that is associated with a strong acid and a cation that is associated with a strong base may be used in such an application. A dimeric dye, such as a dimeric dye capable of forming a hairpin-like structure, may be used to stain and/or detect nucleic acids via a release-on-demand mechanism. A dimeric dye having low background fluorescence in the absence of nucleic acids and high fluorescence in the presence of nucleic acids, upon binding therewith, may be used to stain and/or detect nucleic acids.

摘要翻译： 提供了使用染料和相关技术的方法。 可以在核酸凝胶染色应用和/或核酸检测应用中使用染料，例如单体染料或二聚染料。 这种染料和包含与强酸相关的阴离子和与强碱相关的阳离子的盐可用于这种应用中。 可以使用二聚染料，例如能够形成发夹状结构的二聚染料，以通过按需释放机制对核酸进行染色和/或检测。 在存在核酸时具有低背景荧光的二聚染料和在存在核酸时与核酸存在时的高荧光可用于染色和/或检测核酸。

公开(公告)号：US20080044721A1

公开(公告)日：2008-02-21

申请号：US10427113

申请日：2003-05-01

申请人： Adam Heller ,  Nicholas Mano ,  Hyug-Han Kim ,  Yongchao Zhang ,  Fei Mao ,  Ting Chen ,  Scott Calabrese Barton

发明人： Adam Heller ,  Nicholas Mano ,  Hyug-Han Kim ,  Yongchao Zhang ,  Fei Mao ,  Ting Chen ,  Scott Calabrese Barton

IPC分类号： H01M4/90 ,  C12Q1/00

CPC分类号： H01M8/16 ,  C12Q1/26 ,  C12Q1/32 ,  G01N33/723 ,  Y02E60/527

摘要： A fuel cell is provided with an anode and a cathode. The anode is in electrical communication with an anode enzyme and the cathode is in electrical communication with a cathode enzyme. The anode enzyme is preferably an oxidase or a dehydrogenase. The cathode enzyme is a copper-containing enzyme, such as a laccase, an ascorbate oxidase, a ceruloplasmine, or a bilirubin oxidase. Preferably, the cathode enzyme is operable under physiological conditions. Redox polymers serve to wire the anode enzyme to the anode and the cathode enzyme to the cathode. The fuel cell can be very small in size because it does not require a membrane, seal, or case. The fuel cell can be used in connection with a biological system, such as a human, as it may operate at physiological conditions. By virtue of its size and operability at physiological conditions, the fuel cell is of particular interest for applications calling for a power source implanted in a human body, such as a variety of medical applications.

摘要翻译： 燃料电池设有阳极和阴极。 阳极与阳极酶电连通，阴极与阴极酶电连通。 阳极酶优选为氧化酶或脱氢酶。 阴极酶是含铜酶，例如漆酶，抗坏血酸氧化酶，血清白蛋白或胆红素氧化酶。 优选地，阴极酶在生理条件下是可操作的。 氧化还原聚合物用于将阳极酶连接到阳极，阴极酶连接到阴极。 燃料电池的尺寸可以非常小，因为它不需要膜，密封件或外壳。 燃料电池可以与诸如人类的生物系统结合使用，因为其可以在生理条件下操作。 由于其在生理条件下的尺寸和可操作性，燃料电池对于要求植入人体的电源（例如各种医疗应用）的应用是特别有意义的。

公开(公告)号：US20070193019A1

公开(公告)日：2007-08-23

申请号：US11734979

申请日：2007-04-13

申请人： Benjamin Feldman ,  Adam Heller ,  Ephraim Heller ,  Fei Mao ,  Joseph Vivolo ,  Jeffery Funderburk ,  Fredric Colman ,  Rajesh Krishnan

发明人： Benjamin Feldman ,  Adam Heller ,  Ephraim Heller ,  Fei Mao ,  Joseph Vivolo ,  Jeffery Funderburk ,  Fredric Colman ,  Rajesh Krishnan

IPC分类号： H01S4/00

CPC分类号： G01N27/3272 ,  C12Q1/001 ,  C12Q1/006 ,  C12Q1/32 ,  G01N27/3271 ,  G01N27/3274 ,  Y10T29/49002 ,  Y10T29/49004 ,  Y10T29/49007 ,  Y10T29/49126 ,  Y10T29/49128 ,  Y10T29/4913 ,  Y10T29/49147 ,  Y10T29/49155 ,  Y10T29/49204 ,  Y10T29/49208 ,  Y10T29/4921 ,  Y10T29/49794 ,  Y10T156/1052

摘要： A sensor utilizing a non-leachable or diffusible redox mediator is described. The sensor includes a sample chamber to hold a sample in electrolytic contact with a working electrode, and in at least some instances, the sensor also contains a non-leachable or a diffusible second electron transfer agent. The sensor and/or the methods used produce a sensor signal in response to the analyte that can be distinguished from a background signal caused by the mediator. The invention can be used to determine the concentration of a biomolecule, such as glucose or lactate, in a biological fluid, such as blood or serum, using techniques such as coulometry, amperometry, and potentiometry. An enzyme capable of catalyzing the electrooxidation or electroreduction of the biomolecule is typically provided as a second electron transfer agent.

摘要翻译： 描述了利用不可浸出或可扩散的氧化还原介体的传感器。 传感器包括用于保持样品与工作电极电解接触的样品室，并且在至少一些情况下，传感器还含有不可浸出或可扩散的第二电子转移剂。 传感器和/或所使用的方法响应于能够与介体引起的背景信号区分的分析物产生传感器信号。 本发明可用于使用诸如库仑法，电流分析法和电位法的技术来确定生物流体如血液或血清中的生物分子如葡萄糖或乳酸盐的浓度。 通常可以提供能够催化生物分子的电氧化或电还原的酶作为第二电子转移剂。

公开(公告)号：US07074308B2

公开(公告)日：2006-07-11

申请号：US10714835

申请日：2003-11-14

申请人： Fei Mao ,  Adam Heller

发明人： Fei Mao ,  Adam Heller

IPC分类号： G01N27/327 ,  C07H15/00

CPC分类号： C07F15/0026

摘要： Novel transition metal complexes of iron, cobalt, ruthenium, osmium, and vanadium are described. The transition metal complexes can be used as redox mediators in enzyme-based electrochemical sensors. The transition metal complexes include substituted or unsubstituted (pyridyl)imidazole ligands. Transition metal complexes attached to polymeric backbones are also described.