公开(公告)号：US20150024436A1

公开(公告)日：2015-01-22

申请号：US14381179

申请日：2013-02-28

Applicant: INTEGENX INC.

Inventor： David Eberhart ,  Yuan Li ,  James Ogg ,  Ezra Van Gelder ,  Stephen J. Williams ,  Timothy Woudenberg ,  Dean S. Burgi ,  William D. Nielsen

IPC: C12Q1/68 ,  B01L3/00 ,  B01L7/00 ,  G01N27/447

CPC classification number: C12Q1/686 ,  B01L3/502715 ,  B01L3/5029 ,  B01L3/527 ,  B01L7/52 ,  B01L2200/025 ,  B01L2200/028 ,  B01L2200/04 ,  B01L2200/0647 ,  B01L2200/0684 ,  B01L2200/143 ,  B01L2300/023 ,  B01L2300/027 ,  B01L2300/0672 ,  B01L2300/123 ,  B01L2400/0421 ,  B01L2400/043 ,  B01L2400/0478 ,  B01L2400/0616 ,  G01N27/44704 ,  G01N2035/00158 ,  C12Q2565/629

Abstract: This disclosure provides an integrated and automated sample-to-answer system that, starting from a sample comprising biological material, generates a genetic profile in less than two hours. In certain embodiments, the biological material is DNA and the genetic profile involves determining alleles at one or a plurality of loci (e.g., genetic loci) of a subject, for example, an STR (short tandem repeat) profile, for example as used in the CODIS system. The system can perform several operations, including (a) extraction and isolation of nucleic acid; (b) amplification of nucleotide sequences at selected loci (e.g., genetic loci); and (c) detection and analysis of amplification product. These operations can be carried out in a system that comprises several integrated modules, including an analyte preparation module; a detection and analysis module and a control module.

Abstract translation: 本公开提供了一种综合和自动化的样本回答系统，其从包含生物材料的样品开始，在少于两小时内产生遗传图谱。 在某些实施方案中，生物材料是DNA，并且遗传图谱涉及确定受试者的一个或多个位点（例如，遗传基因座）等位基因，例如STR（短串联重复）分布，例如如 CODIS系统 该系统可以执行若干操作，包括（a）提取和分离核酸; （b）在选择的位点（例如遗传基因座）扩增核苷酸序列; 和（c）扩增产物的检测和分析。 这些操作可以在包括多个集成模块的系统中进行，包括分析物准备模块; 检测和分析模块和控制模块。

公开(公告)号：US08562918B2

公开(公告)日：2013-10-22

申请号：US13717585

申请日：2012-12-17

Applicant: IntegenX Inc.

Inventor： Stevan B. Jovanovich ,  William D. Nielsen ,  David S. Cohen ,  Michael Recknor ,  Mattias Vangbo ,  Ezra Van Gelder ,  Lars Majlof ,  Omar El-Sissi

IPC: G01N15/06 ,  G01N33/00 ,  G01N33/48

CPC classification number: C12Q1/6806 ,  G01J3/4406 ,  G01N1/31 ,  G01N1/405 ,  G01N21/64 ,  G01N27/44721 ,  G01N27/44791 ,  G01N27/745 ,  G01N35/00029 ,  G01N2035/00148 ,  G01N2035/00247 ,  G01N2201/06113 ,  G01N2201/062 ,  Y10T436/11 ,  Y10T436/143333 ,  Y10T436/25 ,  Y10T436/2575

Abstract: The invention provides a system that can process a raw biological sample, perform a biochemical reaction and provide an analysis readout. For example, the system can extract DNA from a swab, amplify STR loci from the DNA, and analyze the amplified loci and STR markers in the sample. The system integrates these functions by using microfluidic components to connect what can be macrofluidic functions. In one embodiment the system includes a sample purification module, a reaction module, a post-reaction clean-up module, a capillary electrophoresis module and a computer. In certain embodiments, the system includes a disposable cartridge for performing analyte capture. The cartridge can comprise a fluidic manifold having macrofluidic chambers mated with microfluidic chips that route the liquids between chambers. The system fits within an enclosure of no more than 10 ft3. and can be a closed, portable, and/or a battery operated system. The system can be used to go from raw sample to analysis in less than 4 hours.

公开(公告)号：US20130224846A1

公开(公告)日：2013-08-29

申请号：US13717585

申请日：2012-12-17

Applicant: IntegenX Inc.

Inventor： Stevan B. Jovanovich ,  William D. Nielsen ,  David S. Cohen ,  Michael Recknor ,  Mattias Vangbo ,  Ezra Van Gelder ,  Lars Majlof ,  Omar El-Sissi

IPC: G01N1/31

CPC classification number: C12Q1/6806 ,  G01J3/4406 ,  G01N1/31 ,  G01N1/405 ,  G01N21/64 ,  G01N27/44721 ,  G01N27/44791 ,  G01N27/745 ,  G01N35/00029 ,  G01N2035/00148 ,  G01N2035/00247 ,  G01N2201/06113 ,  G01N2201/062 ,  Y10T436/11 ,  Y10T436/143333 ,  Y10T436/25 ,  Y10T436/2575

Abstract: The invention provides a system that can process a raw biological sample, perform a biochemical reaction and provide an analysis readout. For example, the system can extract DNA from a swab, amplify STR loci from the DNA, and analyze the amplified loci and STR markers in the sample. The system integrates these functions by using microfluidic components to connect what can be macrofluidic functions. In one embodiment the system includes a sample purification module, a reaction module, a post-reaction clean-up module, a capillary electrophoresis module and a computer. In certain embodiments, the system includes a disposable cartridge for performing analyte capture. The cartridge can comprise a fluidic manifold having macrofluidic chambers mated with microfluidic chips that route the liquids between chambers. The system fits within an enclosure of no more than 10 ft3. and can be a closed, portable, and/or a battery operated system. The system can be used to go from raw sample to analysis in less than 4 hours.

Abstract translation: 本发明提供一种可以处理原始生物样品，进行生化反应并提供分析读数的系统。 例如，该系统可以从拭子中提取DNA，从DNA扩增STR基因座，并分析样品中扩增的位点和STR标记。 该系统通过使用微流体组件来集成这些功能，以连接什么是宏流动功能。 在一个实施方案中，该系统包括样品纯化模块，反应模块，反应后净化模块，毛细管电泳模块和计算机。 在某些实施例中，该系统包括用于执行分析物捕获的一次性盒。 墨盒可以包括流体歧管，该流体歧管具有与微流体芯片配合的大流动腔室，所述微流体芯片在腔室之间引导液体。 该系统适合不超过10英尺3的外壳。 并且可以是封闭的，便携式的和/或电池供电的系统。 该系统可用于在不到4小时内从原始样品进行分析。

公开(公告)号：US20250163493A1

公开(公告)日：2025-05-22

申请号：US18958881

申请日：2024-11-25

Applicant: IntegenX Inc.

Inventor： David Eberhart ,  William D. Nielsen ,  Helen Franklin ,  Stevan B. Jovanovich ,  Dennis Lehto ,  Kaiwan Chear ,  James Klevenberg ,  Chungsoo Charles Park ,  Corey M. Smith ,  Philip Justus Wunderle ,  David King

IPC: C12Q1/6806 ,  B01L3/00 ,  C12Q1/686 ,  G01N27/447

Abstract: An instrument includes a fluidic cartridge having a body comprising a malleable material and a layer comprising a deformable material bonded to a surface of the body that seals one or more fluidic channels that communicate with one or more valve bodies formed in a surface of the body. The valve can be closed by applying pressure to the deformable material sufficient to close off a fluidic channel in the body. A cartridge interface is configured to engage the cartridge.

公开(公告)号：US20250069174A1

公开(公告)日：2025-02-27

申请号：US18943248

申请日：2024-11-11

Applicant: IntegenX Inc.

Inventor： Robert A. Schueren ,  David King ,  Stevan B. Jovanovich

IPC: G06Q50/26 ,  G06F16/11 ,  G06Q10/0631 ,  G06Q30/0601 ,  G06Q50/00 ,  G06Q50/18 ,  G16B50/00 ,  H04W4/12 ,  H04W36/30 ,  H04W56/00 ,  H04W72/21 ,  H04W76/19 ,  H04W76/27

Abstract: Provided herein is a system and method for review of forensic report computer files. The can include a logic processor with a memory or storage configured with logic instructions to provide or facilitate: a data-accessing module for reading and reviewing an original forensic biological data file; an evaluation module for determining whether the original forensic biological data file meets quality criteria for uploading to a forensic matching or identification service or database; and a crowd-sourcing expert review module for communicating with one or more experts for review of the data file when quality criteria or not met and receiving notifications and results from the one or more experts.

公开(公告)号：US20240418675A1

公开(公告)日：2024-12-19

申请号：US18814847

申请日：2024-08-26

Applicant: IntegenX, Inc.

Inventor： Alexander Kindwall ,  Chungsoo Charles Park ,  Fedor Ilkov ,  Stevan Jovanovich ,  David King ,  Tristan Robert Littlehale ,  David Grant Nilson ,  Rick Pittaro ,  Robert A. Schueren ,  Mattias Vangbo ,  Philip Justus Wunderle

IPC: G01N27/447 ,  B01L3/00 ,  B01L7/00 ,  G01N27/453

Abstract: A method includes providing a system comprising an electrophoresis cartridge interface that releasably engages with an electrophoresis cartridge that includes an anode, a cathode, and at least one electrophoresis capillary that is in fluid communication with the anode and the cathode. The method further includes receiving the electrophoresis cartridge at said electrophoresis cartridge interface and automatically establishing at least one of (i) optical communication between an optical detection assembly of the system and a portion of the at least one electrophoresis capillary, (ii) electrical communication between the system and the anode and cathode, (iii) fluidic communication between the system and the at least one electrophoresis capillary, (iv) thermal communication between the system and the electrophoresis cartridge or the at least one electrophoresis capillary; (v) electromagnetic communication between the system and the electrophoresis cartridge and (vi) magnetic communication between the system and the electrophoresis cartridge.

公开(公告)号：US20230409358A1

公开(公告)日：2023-12-21

申请号：US18326519

申请日：2023-05-31

Applicant: IntegenX, Inc.

Inventor： David King ,  Chungsoo Charles Park

IPC: G06F9/451 ,  G06F3/0487 ,  G06Q30/016

CPC classification number: G06F9/453 ,  G06F3/0487 ,  G06Q30/016

Abstract: A live help provider graphical user interface depicting a provider graphical representation of a client device located at a user location and respective provider graphical representations of additional devices located at the user location in a manner that identifies each of the additional devices and depicts a spatial relationship of each of the additional devices relative to the client device at the user location.

公开(公告)号：US11693677B2

公开(公告)日：2023-07-04

申请号：US17574114

申请日：2022-01-12

Applicant: INTEGENX INC.

Inventor： David King ,  Chungsoo Charles Park

IPC: G06F3/0481 ,  G06F9/451 ,  G06F3/0487 ,  G06Q30/016

CPC classification number: G06F9/453 ,  G06F3/0487 ,  G06Q30/016

Abstract: A live help system provides an intuitive display of help information on a user's graphical user interface. A request is received from a client device for help, and a live help provider interface is initiated at a live help location. Data is acquired regarding a user's location, including data on external devices in the user's location. Indicators are provided to allow the live help provider to point to or otherwise indicate items on the user interface or outside of the user interface. Live help input is captured at the live help provider interface. Instructions are then transmitted to the display of the client device to display live help input, as though the agent were present and interacting with or indicating items on the screen or off the screen.

公开(公告)号：US20230152274A1

公开(公告)日：2023-05-18

申请号：US18157274

申请日：2023-01-20

Applicant: IntegenX, Inc.

Inventor： Mattias Vangbo ,  David King ,  Arnaldo Barican

IPC: G01N27/447 ,  C12Q1/6806 ,  C12Q1/686

CPC classification number: G01N27/44713 ,  C12Q1/6806 ,  C12Q1/686 ,  G01N27/44791 ,  C12Q2565/125 ,  G01N27/44721

Abstract: A system for performing capillary electrophoresis of multiple samples comprises a capillary containing a separation medium and having inlet and distal ends and an interrogation region; a power source configured to apply voltages between inlet and distal ends; and logic to cause execution of: applying a first substantially constant forward polarity electrophoresis voltage to the capillary; before all of the first DNA fragments have passed the interrogation region, applying a reverse polarity voltage pulse to the capillary, thereby transporting at least some of the first DNA fragments in the capillary toward the capillary inlet; introducing a second sample to the capillary inlet, the second sample comprising second DNA fragments having a plurality of different sizes; and applying a second substantially constant forward polarity electrophoresis voltage to the capillary to simultaneously perform electrophoresis on the second DNA fragments and the first DNA fragments.

公开(公告)号：US11619608B2

公开(公告)日：2023-04-04

申请号：US16615804

申请日：2018-05-22

Applicant: LIFE TECHNOLOGIES CORPORATION ,  INTEGENX INC.

Inventor： Jen-Kuei Liu ,  Bharti Solanki ,  Hans Michael Wenz ,  Frank Mercer ,  Cevat Akin ,  Achim Karger ,  Annelise Barron ,  Scott Mack

IPC: C02F1/40 ,  C02F11/00 ,  C08G61/04 ,  G01N27/447 ,  C08L33/26

Abstract: Provided herein is an electrophoresis separation medium comprising: (a) a non-crosslinked or sparsely cross-linked polymer or copolymer; (b) one or more denaturant compounds, in an amount sufficient to inhibit re-naturation of single stranded polynucleotides; (c) an aqueous solvent; (d) optionally, a wall-coating material suited to inhibition of electroosmotic flow; and (e) optionally, an organic water miscible solvent such as DMSO or acetonitrile, wherein the electrophoresis separation medium exhibits functional stability for at least seven days at 23° C. Also provided herein are sieving compositions, including polymer-based sieving compositions, for molecular sieving as well as related kits, devices and methods of use. Such compositions can be useful for separation of biomolecules such as nucleic acids, proteins, glycoproteins and glycans.