公开(公告)号：US20180296703A1

公开(公告)日：2018-10-18

申请号：US15753003

申请日：2016-08-17

Applicant: TEMPLE UNIVERSITY OF THE COMMONWEALTH SYSTEM OF HIGHER EDUCATION

Inventor： Arthur M. FELDMAN ,  Joseph Y. CHEUNG

IPC: A61K48/00 ,  C07K14/47 ,  A61P9/10 ,  C12N15/85 ,  C07K19/00

Abstract: The present invention features methods and compositions for treatment of heart failure. The compositions can include an isolated nucleic acid encoding a BAG3 polypeptide or fragment thereof.

公开(公告)号：US20180228876A1

公开(公告)日：2018-08-16

申请号：US15950236

申请日：2018-04-11

Applicant: Temple University of the Commonwealth System of Higher Education

Inventor： Kamel Khalili ,  Wenhui Hu

IPC: A61K38/46 ,  A61K9/00 ,  C12N9/22 ,  C12N7/00 ,  C12N15/11 ,  A61K45/06 ,  A61K48/00 ,  A61K35/12

CPC classification number: A61K38/465 ,  A61K9/0034 ,  A61K35/12 ,  A61K45/06 ,  A61K48/00 ,  A61K48/005 ,  C12N7/00 ,  C12N9/22 ,  C12N15/111 ,  C12N2310/20 ,  C12N2320/30 ,  C12N2740/16063 ,  C12Y301/21

Abstract: A method of preventing transmission of a retrovirus from a mother to her offspring, by administering to the mother a therapeutically effective amount of a composition comprising a Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR)-associated endonuclease, and the two or more different multiplex gRNAs, wherein each of the at least two gRNAs is complementary to a different target nucleic acid sequence in a long terminal repeat (LTR) of proviral DNA of the virus that is unique from the genome of the host cell, cleaving a double strand of the proviral DNA at a first target protospacer sequence with the CRISPR-associated endonuclease, cleaving a double strand of the proviral DNA at a second target protospacer sequence with the CRISPR-associated endonuclease, excising an entire HIV-1 proviral genome, eradicating the HIV-1 proviral DNA from the host cell, and preventing transmission of the proviral DNA to the offspring.

公开(公告)号：US20180200343A1

公开(公告)日：2018-07-19

申请号：US15902263

申请日：2018-02-22

Applicant: Temple University of the Commonwealth System of Higher Education

Inventor： Kamel KHALILI ,  Wenhui HU

IPC: A61K38/46 ,  C12N7/00 ,  A61K9/00 ,  A61K35/12 ,  A61K45/06 ,  C12N15/11 ,  C12N9/22 ,  A61K48/00

CPC classification number: A61K38/465 ,  A61K9/0034 ,  A61K35/12 ,  A61K45/06 ,  A61K48/00 ,  A61K48/005 ,  C12N7/00 ,  C12N9/22 ,  C12N15/111 ,  C12N2310/20 ,  C12N2320/30 ,  C12N2740/16063 ,  C12Y301/21

Abstract: A method of treating a subject having or at risk for having a virus infection, by administering a therapeutically effective amount of a composition comprising a vector encoding a CRISPR-associated endonuclease and at least two guide RNAs that are complementary to two target sequences spanning from the 5′- to 3′-LTRs of the sequence in the virus, and completely excising a fragment of greater than 9000-bp of integrated proviral DNA that spanned from its 5′- to 3′-LTRs. A method of treating a subject having or at risk for having a genetic caused disease, by administering a therapeutically effective amount of a composition comprising a vector encoding a CRISPR-associated endonuclease and at least two guide RNAs that are complementary to two target sequences spanning from the sequence of the subjects DNA greater than 9000-bp that is chromosomally integrated and causes the genetic caused disease, and excising the chromosomally integrated sequence.

公开(公告)号：US20180169193A1

公开(公告)日：2018-06-21

申请号：US15879877

申请日：2018-01-25

Applicant: Temple University of the Commonwealth System of Higher Education

Inventor： Kamel KHALILI ,  Wenhui HU

IPC: A61K38/46 ,  C12N15/11 ,  A61K35/12 ,  A61K48/00 ,  C12N7/00 ,  C12N9/22 ,  A61K9/00 ,  A61K45/06

CPC classification number: A61K38/465 ,  A61K9/0034 ,  A61K35/12 ,  A61K45/06 ,  A61K48/00 ,  A61K48/005 ,  C12N7/00 ,  C12N9/22 ,  C12N15/111 ,  C12N2310/20 ,  C12N2320/30 ,  C12N2740/16063 ,  C12Y301/21

Abstract: Methods of inactivating a proviral DNA genome or a DNA genome integrated into the genome of a host cell latently infected with a retrovirus, by treating the host cell with a composition comprising a Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR)-associated endonuclease, and two or more different multiplex guide RNAs (gRNAs), wherein each of the at least two gRNAs is complementary to a different target nucleic acid sequence in a long terminal repeat (LTR) of the proviral DNA that is unique from the genome of the host cell, cleaving a double strand of the proviral DNA at a first target protospacer sequence with the CRISPR-associated endonuclease, cleaving a double strand of the proviral DNA at a second target protospacer sequence with the CRISPR-associated endonuclease, excising an entire proviral genome of the proviral DNA, and eradicating the proviral DNA from the host cell.

公开(公告)号：US09925248B2

公开(公告)日：2018-03-27

申请号：US14838057

申请日：2014-08-29

Applicant: TEMPLE UNIVERSITY OF THE COMMONWEALTH SYSTEM OF HIGHER EDUCATION

Inventor： Kamel Khalili ,  Wenhui Hu

IPC: A61K48/00 ,  A61K38/46 ,  A61K9/00 ,  A61K35/12 ,  A61K45/06 ,  C12N7/00 ,  C12N9/22 ,  C12N15/11 ,  A61K39/21 ,  C12N15/55 ,  C12N15/79 ,  C12N15/85 ,  C12N15/86

CPC classification number: A61K38/465 ,  A61K9/0034 ,  A61K35/12 ,  A61K45/06 ,  A61K48/00 ,  A61K48/005 ,  C12N7/00 ,  C12N9/22 ,  C12N15/111 ,  C12N2310/20 ,  C12N2320/30 ,  C12N2740/16063 ,  C12Y301/21

Abstract: A method of inactivating a proviral DNA integrated into the genome of a host cell latently infected with a retrovirus by treating the host cell with a composition comprising a Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR)-associated endonuclease, and two or more different guide RNAs (gRNAs), wherein each of the at least two gRNAs is complementary to a different target nucleic acid sequence in a long terminal repeat (LTR) in the proviral DNA, and inactivating the proviral DNA. A composition for use in inactivating a proviral DNA integrated into the genome of a host cell latently infected with a retrovirus including isolated nucleic acid sequences comprising a CRISPR-associated endonuclease and a guide RNA, wherein the guide RNA is complementary to a target sequence in a human immunodeficiency virus.

公开(公告)号：US20180073019A1

公开(公告)日：2018-03-15

申请号：US15559902

申请日：2016-03-18

Applicant: Temple University - of the Commonwealth System of Higher Education

Inventor： Kamel Khalili

IPC: C12N15/11 ,  C12N9/22 ,  A61K48/00 ,  C12N15/86 ,  A61K45/06 ,  C12N7/00

CPC classification number: C12N15/111 ,  A61K45/06 ,  A61K48/005 ,  A61K48/0075 ,  C12N7/00 ,  C12N9/22 ,  C12N15/11 ,  C12N15/1132 ,  C12N15/86 ,  C12N2310/20 ,  C12N2310/3519 ,  C12N2320/30 ,  C12N2740/16062 ,  C12N2800/107 ,  C12N2800/22 ,  C12N2830/00 ,  C12N2830/30 ,  C12N2830/60

Abstract: Compositions and methods are provided for Tat-inducible expression of a CRISPR-associated endonuclease by a truncated HIV LTR promoter containing at least a core region and a TAR region of a HIV LTR promoter. The compositions may be used as a therapeutic treatment for the treatment and/or prevention of HIV.

公开(公告)号：US20180008565A1

公开(公告)日：2018-01-11

申请号：US15545371

申请日：2016-01-21

Applicant: Helena M.G.P.V. REIS ,  Temple University-Of The Commonwealth System of Higher Education

Inventor： Mark A. Feitelson ,  Helena M.G.P.V. Reis

IPC: A61K31/19 ,  A61K9/00

CPC classification number: A61K31/19 ,  A61K9/0053 ,  A61K35/744 ,  A61K35/747 ,  A61P35/00

Abstract: The invention relates to compositions for preventing or delaying the onset of hepatocellular cancer. The compositions of the invention may comprise short chain fatty acids. The compositions of the invention may also comprise probiotic bacteria. The compositions of the invention include compositions for preventing or delaying the onset of hepatocellular cancer by treating or preventing liver inflammation, liver disease, and precancerous lesions.

公开(公告)号：US20170349628A1

公开(公告)日：2017-12-07

申请号：US15515416

申请日：2015-10-02

Applicant: Temple University-Of The Commonwealth System of Higher Education

Inventor： Won Hyuk Suh ,  Geunwoo Jin

IPC: C07K7/06 ,  A61K9/107 ,  A61K31/519 ,  C12N5/0793 ,  A61K47/64 ,  A61K38/08 ,  A61K31/203

CPC classification number: C07K7/06 ,  A61K9/107 ,  A61K31/203 ,  A61K31/519 ,  A61K38/08 ,  A61K47/64 ,  C07K2319/10 ,  C07K2319/60 ,  C12N5/0619 ,  C12N2501/385

Abstract: The present invention provides a novel cell penetrating peptide that transports proteins into cells and/or into nuclei and a pharmaceutical containing the peptide.

公开(公告)号：US09828458B2

公开(公告)日：2017-11-28

申请号：US14350658

申请日：2012-10-17

Applicant: TEMPLE UNIVERSITY—OF THE COMMONWEALTH SYSTEM OF HIGHER EDUCATION

Inventor： Rominder P. S. Suri ,  Bikash Bhattarai

IPC: B01J20/283 ,  B01J20/32 ,  B01J20/286 ,  B01J20/10 ,  C02F1/28 ,  C08G18/64 ,  B01J20/288 ,  C02F1/68 ,  C07H23/00 ,  B01J20/26 ,  C02F101/30

CPC classification number: C08G18/6484 ,  B01J20/267 ,  B01J20/286 ,  B01J20/288 ,  B01J20/3204 ,  B01J20/3274 ,  B01J20/3282 ,  B01J20/3293 ,  C02F1/281 ,  C02F1/285 ,  C02F1/288 ,  C02F1/683 ,  C02F2101/305 ,  C07H23/00

Abstract: Provided is a silica particle coated with β-cyclodextrin, wherein said cyclodextrin is attached to said silica particle via at least one crosslinking agent and/or at least one copolymer. Also provided are methods of removing contaminants from a flowing or stationary liquid phase comprising the step of contacting said liquid phase with the silica particle coated with β-cyclodextrin.

公开(公告)号：US20170174734A1

公开(公告)日：2017-06-22

申请号：US15125384

申请日：2015-03-12

Applicant: Temple University-Of The Commonwealth System of Higher Education

Inventor： George P. Tuszynski

IPC: C07K14/47 ,  G01N33/574 ,  C07K16/18

CPC classification number: C07K14/4747 ,  A61K38/00 ,  C07K16/18 ,  C07K2317/76 ,  G01N33/57407 ,  G01N33/57415 ,  G01N33/57426 ,  G01N33/5743

Abstract: The present invention provides compositions and methods of treating cancer by inducing the cellular differentiation activity of angiocidin.