公开(公告)号：US12038372B2

公开(公告)日：2024-07-16

申请号：US18198478

申请日：2023-05-17

Applicant: BECTON, DICKINSON AND COMPANY

Inventor： Eric D. Diebold ,  Keegan Owsley ,  Jonathan Lin

IPC: G01N15/1434 ,  G01N15/14 ,  G01N15/1429 ,  G01N15/1433 ,  G01N21/64 ,  G02B21/06 ,  G01N15/10

CPC classification number: G01N15/1434 ,  G01N15/14 ,  G01N15/1425 ,  G01N15/1429 ,  G01N15/1433 ,  G01N15/147 ,  G01N21/645 ,  G02B21/06 ,  G01N2015/1006 ,  G01N2015/1447 ,  G01N2015/1454 ,  G01N2021/6482

Abstract: Aspects of the disclosure include a system for performing cytometry that can be operated in three operational modes. In one operational mode, a fluorescence image of a sample is obtained by exciting one or more fluorophore(s) with a top-hat-shaped excitation beam with a plurality of beams that are radiofrequency shifted relative to one another. In another operational mode, a sample can be illuminated successively over a time interval by a laser beam at a plurality of excitation frequencies in a scanning fashion to generate an image of the sample from the detected fluorescence. In another operational mode, the system can be operated to illuminate a plurality of locations of a sample concurrently by a single excitation frequency (e.g., a horizontal extent), which is generated, e.g., by shifting the central frequency of a laser beam by a radiofrequency and detected fluorescence is used to analyze the sample.

公开(公告)号：US12019006B2

公开(公告)日：2024-06-25

申请号：US18139721

申请日：2023-04-26

Applicant: Becton, Dickinson and Company

Inventor： Jonathan Lin ,  Keegan Owsley ,  David A. Roberts

IPC: G01N15/1429 ,  G01N15/10 ,  G01N15/14 ,  G01N15/1404 ,  G01N15/149 ,  G06F18/213 ,  G06F18/23

CPC classification number: G01N15/1429 ,  G01N15/1456 ,  G01N15/1459 ,  G06F18/213 ,  G06F18/23 ,  G01N2015/1006 ,  G01N2015/1402 ,  G01N15/1404 ,  G01N2015/1477 ,  G01N2015/1488 ,  G01N15/149

Abstract: Disclosed herein include systems, devices, computer readable media, and methods for subsampling flow cytometric event data. First and second flow cytometric event data can be transformed into a lower-dimensional space, associated with a plurality of bins, and assigned to a first bin and a second bin. Subsampled flow cytometric event data comprising the first flow cytometric event data can be generated. The subsampled flow cytometric event data can comprise the second flow cytometric event data if the first bin and the second bin are different. The subsampled flow cytometric event data may not comprise the second flow cytometric event data if the first bin and the second bin are identical.

公开(公告)号：US11988589B2

公开(公告)日：2024-05-21

申请号：US17588040

申请日：2022-01-28

Applicant: Becton, Dickinson and Company

Inventor： Matthew Bahr ,  Eric D. Diebold ,  Jonathan Lin ,  Keegan Owsley

IPC: G01N15/0227 ,  G01N15/1404

CPC classification number: G01N15/0227 ,  G01N15/1404

Abstract: Aspects of the present disclosure include methods for characterizing particles of a sample in a flow stream. Methods according to certain embodiments include detecting light from a sample having cells in a flow stream, generating an image of an object in the flow stream in an interrogation region and determining whether the object in the flow stream is an aggregate based on the generated image. Systems having a processor with memory operably coupled to the processor having instructions stored thereon, which when executed by the processor, cause the processor to generate an image of an object in a flow stream and to determine whether the object is an aggregate are also described. Integrated circuit devices (e.g., field programmable gate arrays) having programming for practicing the subject methods are also provided.

公开(公告)号：US20230296494A1

公开(公告)日：2023-09-21

申请号：US18199756

申请日：2023-05-19

Applicant: Becton, Dickinson and Company

Inventor： Eric D. Diebold ,  Keegan Owsley ,  Matthew Bahr

IPC: G01N15/14 ,  G02F1/33 ,  H01S3/00

CPC classification number: G01N15/1434 ,  G01N15/1459 ,  G02F1/33 ,  H01S3/0071 ,  G01N2015/145 ,  G01N2015/1006

Abstract: Aspects of the present disclosure include methods for producing an output laser beam having two or more angularly deflected laser beams (e.g., for irradiating a sample in a flow stream) with a predetermined intensity profile. Systems for practicing the subject methods having a laser, an acousto-optic device, a radiofrequency generator and a controller for adjusting the amplitude of the radiofrequency drive signals to produce an output laser beam of angularly deflected laser beams with a predetermined intensity profile are also described.

公开(公告)号：US20230288311A1

公开(公告)日：2023-09-14

申请号：US18198478

申请日：2023-05-17

Applicant: BECTON, DICKINSON AND COMPANY

Inventor： Eric D. Diebold ,  Keegan Owsley ,  Jonathan Lin

IPC: G01N15/14 ,  G01N21/64 ,  G02B21/06

CPC classification number: G01N15/1434 ,  G01N15/14 ,  G01N15/1425 ,  G01N15/147 ,  G01N15/1475 ,  G01N15/1429 ,  G01N21/645 ,  G02B21/06 ,  G01N2015/1447 ,  G01N2015/1454 ,  G01N2015/1006

Abstract: In one aspect, the present teachings provide a system for performing cytometry that can be operated in three operational modes. In one operational mode, a fluorescence image of a sample is obtained by exciting one or more fluorophore(s) present in the sample by an excitation beam formed as a superposition of a top-hat-shaped beam with a plurality of beams that are radiofrequency shifted relative to one another. In another operational mode, a sample can be illuminated successively over a time interval by a laser beam at a plurality of excitation frequencies in a scanning fashion. The fluorescence emission from the sample can be detected and analyzed, e.g., to generate a fluorescence image of the sample. In yet another operational mode, the system can be operated to illuminate a plurality of locations of a sample concurrently by a single excitation frequency, which can be generated, e.g., by shifting the central frequency of a laser beam by a radiofrequency. For example, a horizontal extent of the sample can be illuminated by a laser beam at a single excitation frequency. The detected fluorescence radiation can be used to analyze the fluorescence content of the sample, e.g., a cell/particle.

公开(公告)号：US20230258551A1

公开(公告)日：2023-08-17

申请号：US18139721

申请日：2023-04-26

Applicant: Becton, Dickinson and Company

Inventor： Jonathan Lin ,  Keegan Owsley ,  David A. Roberts

IPC: G01N15/14 ,  G06F18/23 ,  G06F18/213

CPC classification number: G01N15/1429 ,  G01N15/1456 ,  G01N15/1459 ,  G06F18/23 ,  G06F18/213 ,  G01N2015/149 ,  G01N2015/1402 ,  G01N15/1404 ,  G01N2015/1477 ,  G01N2015/1488 ,  G01N2015/1006

Abstract: Disclosed herein include systems, devices, computer readable media, and methods for subsampling flow cytometric event data. First and second flow cytometric event data can be transformed into a lower-dimensional space, associated with a plurality of bins, and assigned to a first bin and a second bin. Subsampled flow cytometric event data comprising the first flow cytometric event data can be generated. The subsampled flow cytometric event data can comprise the second flow cytometric event data if the first bin and the second bin are different. The subsampled flow cytometric event data may not comprise the second flow cytometric event data if the first bin and the second bin are identical.

公开(公告)号：US20230243736A1

公开(公告)日：2023-08-03

申请号：US18130571

申请日：2023-04-04

Applicant: Becton, Dickinson and Company

Inventor： Jonathan Lin ,  Joseph T. Trotter ,  Keegan Owsley

IPC: G01N15/14 ,  G16C20/20 ,  B07C5/342 ,  B07C5/36 ,  G06V20/69 ,  G06V10/70

CPC classification number: G01N15/1459 ,  G16C20/20 ,  G01N15/1425 ,  G01N15/1434 ,  B07C5/3425 ,  B07C5/361 ,  G06V20/695 ,  G06V20/698 ,  G06V10/87 ,  G01N2015/149

Abstract: A cell sorting system that automatically generates a sorting strategy based on examples of target events provided by an operator. The target events can be selected using measurements ranging from traditional flow cytometry measurements to derived measurements that are computationally expensive to complex measurements such as images.

公开(公告)号：US11662297B2

公开(公告)日：2023-05-30

申请号：US17226681

申请日：2021-04-09

Applicant: Becton, Dickinson and Company

Inventor： David Alexander Roberts ,  Lingjie Wei ,  Peter Mage ,  Keegan Owsley

IPC: G01N15/14

CPC classification number: G01N15/147 ,  G01N15/1434 ,  G01N2015/1447 ,  G01N2015/1493

Abstract: Aspects of the present disclosure include methods for flow cytometrically sorting a sample with particles, such as cells, based on order of identification. Methods according to certain embodiments include introducing the sample into a flow cytometer; flowing the introduced sample in a flow stream; irradiating the sample in the flow stream with a light source; detecting light from cells in the sample flowing in the flow stream; identifying phenotypes of cells in the sample flowing in the flow stream based on one or more data signals generated from the detected light; and dynamically sorting into partitions cells of the sample that have a phenotype of a collection of predetermined phenotypes based on order of identification. Systems for practicing the subject methods are also provided. Non-transitory computer readable storage mediums are also described.

公开(公告)号：US20230152208A1

公开(公告)日：2023-05-18

申请号：US17984931

申请日：2022-11-10

Applicant: Becton, Dickinson and Company

Inventor： Keegan Owsley ,  Christopher J. Wolf

IPC: G01N15/14

CPC classification number: G01N15/1475 ,  G01N15/147 ,  G01N2015/149

Abstract: Aspects of the present disclosure include methods for dynamic real-time adjustment of data acquisition parameters of a particle analyzer. Methods according to certain embodiments include detecting light from a particle of a sample in a flow stream irradiated with a light source, generating an image of the particle based on the detected light and automatically adjusting a data acquisition parameter of the particle analyzer in response to a modulated visualization parameter for the image of the particle. Systems (e.g., particle analyzers) having a light source and a light detection system that includes an imaging photodetector and processor with memory having instructions for practicing the subject methods are also described. Non-transitory computer readable storage medium is also provided.

公开(公告)号：US11625829B2

公开(公告)日：2023-04-11

申请号：US17714875

申请日：2022-04-06

Applicant: Becton, Dickinson and Company

Inventor： Matthew Bahr ,  Keegan Owsley

IPC: G06T7/00 ,  G01N15/14 ,  G01N21/64 ,  C12Q1/02

Abstract: Disclosed herein include embodiments of a system, a device, and a method for sorting a plurality cells of a sample. A plurality of raw images comprising pixels of complex values in a frequency space can be generated from a plurality of channels of fluorescence intensity data of fluorescence emissions of fluorophores, the fluorescence emissions being elicited by fluorescence imaging using radiofrequency-multiplexed excitation in a temporal space. Spectral unmixing can be performed on the raw images prior to a sorting decision being made.