公开(公告)号：US09879321B2

公开(公告)日：2018-01-30

申请号：US14577063

申请日：2014-12-19

Applicant: E. I. DuPont de Nemours and Company ,  Pioneer Hi-Bred International, Inc. ,  Dow Agrosciences LLC

Inventor： James Wayne Bing ,  Robert F. Cressman, Jr. ,  Manju Gupta ,  Salim M. Hakimi ,  David Hondred ,  Todd L. Krone ,  Mary E. Hartnett Locke ,  Abigail K. Luckring ,  Sandra E. Meyer ,  Daniel Moellenbeck ,  Kenneth Edwin Narva ,  Paul D. Olson ,  Craig D. Sanders ,  Jimei Wang ,  Jian Zhang ,  Gan-Yuan Zhong

IPC: C12Q1/68 ,  C07K14/415 ,  C12N15/82

CPC classification number: C12Q1/6876 ,  C07K14/415 ,  C12N15/8277 ,  C12N15/8286 ,  C12Q1/6895 ,  C12Q2600/13 ,  Y02A40/162

Abstract: The invention provides DNA compositions that relate to transgenic insect resistant maize plants. Also provided are assays for detecting the presence of the maize DAS-59122-7 event based on the DNA sequence of the recombinant construct inserted into the maize genome and the DNA sequences flanking the insertion site. Kits and conditions useful in conducting the assays are provided.

公开(公告)号：US20170298451A1

公开(公告)日：2017-10-19

申请号：US15495937

申请日：2017-04-24

Applicant: Dow AgroSciences LLC

Inventor： Lasantha Chandana Ubayasena ,  Zoe C. Ehlert ,  Manju Gupta ,  Chandra - Shekara A. Channabasavaradhya

IPC: C12Q1/68

CPC classification number: C12Q1/6895 ,  C12Q2600/156

Abstract: The subject disclosure relates in part to endpoint TaqMan® PCR assays for the detection and high throughput zygosity analysis of the fad-3c gene in canola. The subject disclosure further relates, in part, to the use of wild type DNA as a reference for use in determining zygosity. These and other related procedures can be used to uniquely identify the zygosity and variety of canola lines comprising the subject gene. The subject disclosure also provides related kits for determining zygosity from a sample of a canola plant or seed, for example.

公开(公告)号：US09765350B2

公开(公告)日：2017-09-19

申请号：US14844731

申请日：2015-09-03

Applicant: Dow AgroSciences LLC

Inventor： Manju Gupta ,  Sara Bennett ,  Sandeep Kumar ,  Donald Merlo ,  Nagesh Sardesai

IPC: C12N15/82 ,  C12N15/74

CPC classification number: C12N15/8205 ,  C12N15/743

Abstract: The present disclosure provides novel compositions and methods for the production and use of Agrobacterium tumefaciens strains (for example LBA4404) that are deficient in RecA activity relative to the parent strain. Combinations with other gene-deficient-strains of Agrobacterium tumefaciens are also disclosed. Specifically, two exemplary s recA minus strains, UIA777 where chloramphenicol resistant gene disrupting the recA gene and UIA770 where kanamycin resistant gene disrupting the recA gene are provided.

公开(公告)号：US20170204425A1

公开(公告)日：2017-07-20

申请号：US15471545

申请日：2017-03-28

Applicant: Dow AgroSciences LLC ,  Sangamo Therapeutics, Inc.

Inventor： Russell DeKelver ,  Manju Gupta ,  Jeffrey C. Miller ,  Stephen Novak ,  Joseph F. Petolino

IPC: C12N15/82

CPC classification number: C12N15/8218 ,  C07K14/415 ,  C12N9/22 ,  C12N15/8216 ,  C12N15/8247 ,  C12Y203/01041 ,  C12Y301/00

Abstract: The present disclosure relates to engineered zinc finger proteins that target genes in plants involved in fatty acid biosynthesis. Methods of using such zinc finger proteins in modulating gene expression, gene inactivation, and targeted gene modification are also provided.

公开(公告)号：US20170137834A1

公开(公告)日：2017-05-18

申请号：US15331126

申请日：2016-10-21

Applicant: Dow AgroSciences LLC

Inventor： Manju Gupta ,  Sandeep Kumar ,  Navin Elango ,  Jeffrey Beringer ,  Shavell Gorman ,  Andrew F. Worden ,  Sara Bennett ,  Daren Hemingway ,  Wei Chen ,  Huixia Wu ,  Ning Zhou ,  Michelle Sprint Smith

IPC: C12N15/82 ,  C07K14/325

CPC classification number: C12N15/8227 ,  C12N9/14 ,  C12N15/8225 ,  C12N15/8286 ,  C12Y303/01001 ,  Y02A40/162

Abstract: Provided are constructs and methods for expressing a transgene in plant cells and/or plant tissues using Zea mays GRMZM2G015295 gene regulatory elements.

公开(公告)号：US20170088825A1

公开(公告)日：2017-03-30

申请号：US15379152

申请日：2016-12-14

Applicant: Dow AgroSciences LLC ,  Sangamo BioSciences, Inc.

Inventor： Vipula Shukla ,  Manju Gupta ,  Fyodor Urnov ,  Dmitry Y. Guschin ,  Michiel Jan De Both ,  Paul Bundock ,  Lakshmi Sastry-Dent

IPC: C12N9/04 ,  A01C14/00 ,  C12N15/82

CPC classification number: C12Y101/01037 ,  C12N15/8213 ,  C12N15/8241 ,  C12N15/8243 ,  C12N15/8261 ,  Y02A40/146

Abstract: Disclosed herein are methods and compositions for targeted modification of one or more endogenous plant malate dehydrogenase genes.

公开(公告)号：US20160237446A1

公开(公告)日：2016-08-18

申请号：US14604510

申请日：2015-01-23

Applicant: Dow AgroSciences LLC

Inventor： Manju Gupta ,  Sara Bennett ,  Navin Elango ,  Karthik Muthuraman ,  Jeffrey Beringer ,  Huixia Wu

IPC: C12N15/82

CPC classification number: C12N15/8223 ,  C12N15/8218 ,  C12N15/8271 ,  C12N15/8279

Abstract: Provided are constructs and methods for expressing a transgene in plant cells and/or plant tissues using Zea mays chlorophyll a/b binding gene regulatory elements.

Abstract translation: 提供的是使用玉米叶绿素a / b结合基因调控元件在植物细胞和/或植物组织中表达转基因的构建体和方法。

公开(公告)号：US20150176015A1

公开(公告)日：2015-06-25

申请号：US14416799

申请日：2013-07-23

Applicant: DOW AGROSCIENCES LLC

Inventor： Sandeep Kumar ,  Steven L. Evans ,  Manju Gupta

IPC: C12N15/82

CPC classification number: C12N15/8209 ,  C12N15/68 ,  C12N15/82

Abstract: This invention is related to methods and systems for vector assembly for transgenic plants. A uniform modular process is used to reduce cycle time and the methods and systems provided herein can increase cloning throughput using multiple-well plates, for example 96-well plates. In some embodiments, the methods and systems provided herein eliminate or reduce the need for sequencing confirmation because no PCR is involved in the vector assembly process.

Abstract translation: 本发明涉及用于转基因植物的载体组装的方法和系统。 使用统一的模块化方法来减少循环时间，本文提供的方法和系统可以使用多孔板（例如96孔板）提高克隆产量。 在一些实施方案中，本文提供的方法和系统消除或减少对测序确认的需要，因为在载体组装过程中不涉及PCR。

公开(公告)号：US08889390B2

公开(公告)日：2014-11-18

申请号：US13766380

申请日：2013-02-13

Applicant: Dow AgroSciences LLC ,  Sangamo BioSciences, Inc.

Inventor： Manju Gupta ,  Asha M. Palta ,  Stephen Novak ,  Fyodor Urnov ,  Sunita Gopalan

IPC: C12N9/00 ,  C12N15/82 ,  C12N9/10

CPC classification number: C12N15/8213 ,  C07K2319/00 ,  C07K2319/81 ,  C12N9/1092 ,  C12N15/8243 ,  C12N15/8274

Abstract: The present disclosure relates to engineered zinc finger proteins that target 5-enolpyruvyl shikimate-3-phosphate synthase (EPSPS) genes in plants and methods of using such zinc finger proteins in modulating gene expression, gene inactivation, and targeted gene modification. In particular, the disclosure pertains to zinc finger nucleases for targeted cleavage and alteration of EPSPS genes.

Abstract translation: 本公开涉及靶向植物中5-烯醇丙酮酸莽草酸-3-磷酸合酶（EPSPS）基因的工程化锌指蛋白，以及使用这种锌指蛋白调节基因表达，基因失活和靶向基因修饰的方法。 特别地，本公开涉及用于靶向切割和改变EPSPS基因的锌指核酸酶。

公开(公告)号：US20140157458A1

公开(公告)日：2014-06-05

申请号：US14089260

申请日：2013-11-25

Applicant: Sangamo BioSciences, Inc. ,  Dow AgroSciences LLC

Inventor： Russell DeKelver ,  Manju Gupta ,  Jeffrey C. Miller ,  Stephen Novak ,  Joseph F. Petolino

IPC: C12N15/82 ,  C12N9/22 ,  C07K14/415

CPC classification number: C12N15/8218 ,  C07K14/415 ,  C12N9/22 ,  C12N15/8216 ,  C12N15/8247 ,  C12Y203/01041 ,  C12Y301/00

Abstract: The present disclosure relates to engineered zinc finger proteins that target genes in plants involved in fatty acid biosynthesis. Methods of using such zinc finger proteins in modulating gene expression, gene inactivation, and targeted gene modification are also provided.

Abstract translation: 本公开涉及靶向参与脂肪酸生物合成的植物中的基因的工程化锌指蛋白。 还提供了使用这种锌指蛋白调节基因表达，基因失活和靶向基因修饰的方法。