公开(公告)号：US10802002B2

公开(公告)日：2020-10-13

申请号：US15990097

申请日：2018-05-25

Applicant: Waters Technologies Corporation

Inventor： Joseph D. Michienzi ,  Keith Fadgen ,  Wade P. Leveille ,  Raymond P. Fisk ,  Frank John Marszalkowski, Jr.

IPC: G01N30/60 ,  B01D15/10 ,  B01D15/22 ,  B05B5/16 ,  G01N30/72 ,  B01D15/18

Abstract: Described is a chromatographic column assembly that includes an outer tube comprising a metal, a first conduit disposed within the outer tube, a second conduit disposed within the outer tube, and a first joint located between the first conduit and the second conduit. The outer tube is deformed by a first uniform radial crimp at a longitudinal location along the outer tube that surrounds the first conduit on a first side of the first joint, and a second uniform radial cramp at a longitudinal location along the outer tube that surrounds the second conduit on a second side of the first joint. The first and second uniform radial cramps form a fluid-tight seal between the first conduit and the second conduit and each have a substantially flat base region over which a diameter of the outer tube is reduced for a non-zero longitudinal length.

公开(公告)号：US20200217825A1

公开(公告)日：2020-07-09

申请号：US16334118

申请日：2017-09-13

Applicant: Waters Technologies Corporation

Inventor： Joshua Shreve ,  Jason F. Hill ,  Joseph D. Michienzi ,  Abhijit Tarafder ,  Michael O. Fogwill

IPC: G01N30/32 ,  B01D15/40 ,  B01D15/16 ,  B01D15/18 ,  G01N30/60 ,  G01N30/86

Abstract: Methods for transferring a carbon dioxide based separation procedure from a first chromatographic system to a second one involve identifying an average column pressure for the separation in the first system is identified, determining a measured average column pressure for the separation in the second system, and comparing the measured average column pressure with the identified average column pressures. To more closely match the identified average column pressure, the methods involve: (a) altering a cross-sectional area of a column packed with media in the second system; and/or (b) adding makeup fluid along the length of the column in the second system. Columns with the characteristics used in the methods and second chromatographic systems are disclosed.

公开(公告)号：US10705060B2

公开(公告)日：2020-07-07

申请号：US15553268

申请日：2016-02-25

Applicant: Waters Technologies Corporation

Inventor： Michael O. Fogwill ,  Joseph D. Michienzi ,  Martin Gilar ,  Abhijit Tarafder

IPC: G01N30/30 ,  G01N30/34 ,  G01N30/86 ,  B01D15/16 ,  G01N30/46 ,  G01N30/02

Abstract: Methods for focusing analyte peaks in liquid chromatography using a spatial temperature gradient are provided. Also provided are methods for focusing analyte peaks and improving resolution using a trap column upstream of a separation column. Further, methods are provided in which the trap column placed upstream of the separation column is packed with a temperature-sensitive polymer/copolymer, and a spatial temperature gradient is applied along the trap column for obtaining improved retentivity by trap column stationary phase, and overall improved resolution of analyte peaks.

公开(公告)号：US20200064312A1

公开(公告)日：2020-02-27

申请号：US16545590

申请日：2019-08-20

Applicant: Waters Technologies Corporation

Inventor： Jeffrey Musacchio ,  Keith Fadgen ,  Joseph D. Michienzi ,  Stanislaw Koziol

IPC: G01N30/16

Abstract: Described is a fluidic manifold that includes a block formed of multiple layers each bonded to at least one adjacent layer at a layer interface. Bonding may be achieved using a diffusion bonding process. The block includes one or more attachment surfaces and at least two fluidic channels. Each fluidic channel is at least partially disposed at one of the layer interfaces and has a first end at one of the attachment surfaces. Each attachment surface includes an attachment feature at the first end of one of the fluidic channels to enable a fluidic coupling of the two fluidic channels through a fluidic component. Attachment features include, for example, a compression fitting coupling body adapted to receive a conventional seal, such as a ferrule and compression screw, and a fitting body that permits a face seal or gasket seal between the fluidic component and the block.

公开(公告)号：US20190275442A1

公开(公告)日：2019-09-12

申请号：US15915847

申请日：2018-03-08

Applicant: Waters Technologies Corporation

Inventor： Michael O. Fogwill ,  Keith Fadgen ,  Scott Kelley ,  Joseph D. Michienzi

IPC: B01D11/02

Abstract: Exemplary embodiments are directed to a method for diffusing fluid flow within a modular extraction vessel. A modular extraction vessel can be assembled using a plurality of extraction vessel chambers, and each extraction vessel chamber is associated with at least one diffuser element. An extracting solvent is introduced into the modular extraction vessel, and the extracting solvent flows through the extraction vessel chambers. By flowing through the extraction vessel chambers and diffusers, the flow path of the extracting solvent is diffused along an extraction path.

公开(公告)号：US20190257442A1

公开(公告)日：2019-08-22

申请号：US16277737

申请日：2019-02-15

Applicant: Waters Technologies Corporation

Inventor： Michael O. Fogwill ,  Keith Fadgen ,  Scott Kelley ,  Joseph D. Michienzi

IPC: F16K31/126 ,  F16K7/17

Abstract: A pressure control device for use with a compressible fluid extraction system is provided, which includes a body portion integrated with a first vessel, and a pressure control element configured to control a first pressure of a second vessel upstream of the first vessel, wherein a decompression event occurs at a point of decompression proximate an outlet of the pressure control element of the pressure control device, wherein an analyte soluble in an extraction solvent stream at the first pressure but has reduced solubility in the extraction solvent stream at the pressure resulting from the decompression event drops out of solution and into the first vessel at the point of decompression Furthermore, an associated extraction system and method is also provided.

公开(公告)号：US10359403B2

公开(公告)日：2019-07-23

申请号：US15909275

申请日：2018-03-01

Applicant: Waters Technologies Corporation

Inventor： Michael O. Fogwill ,  Joshua A. Shreve ,  Michael Eggertson ,  Thomas S. McDonald ,  Joseph D. Michienzi ,  Abhijit Tarafder

IPC: G01N30/32 ,  G01N30/72 ,  G01N30/34 ,  B01D15/16 ,  B01D15/40

Abstract: The present disclosure relates to methodologies, systems and apparatus for controlling pressure in a CO2-based chromatography system. A first pressure control element is located downstream of a CO2-based chromatography system and is disposed to control pressure within the column. A split restrictor is located downstream of the primary pressure control element and is disposed to divert a portion of the mobile phase flow to a detector. A second pressure control element is located downstream of the split restrictor and is disposed to control pressure at the restrictor. While the first pressure control element executes a pressure-controlled gradient separation, the second pressure control element maintains a constant pressure at the restrictor. During a composition-programmed gradient separation, the second control element maintains a constant pressure at the split restrictor while the first pressure control element maintains a constant average density across the column.

公开(公告)号：US20180292362A1

公开(公告)日：2018-10-11

申请号：US15948653

申请日：2018-04-09

Applicant: Waters Technologies Corporation

Inventor： Joseph D. Michienzi ,  Joshua A. Shreve ,  Jason F. Hill ,  Abhijit Tarafder ,  Michael O. Fogwill ,  Keith Fadgen

IPC: G01N30/38 ,  B01D15/40 ,  B01D15/16 ,  G01N30/46 ,  G01N30/32

Abstract: Methods for transferring a carbon dioxide based separation procedure from a reference chromatographic system to a target chromatographic system involve alternative techniques for determining system pressure drops not attributable to the column. One technique involves leveraging experimental chromatography to develop a correction factor that is a function of at least one correction coefficient and at least one ratio of the differential analyte retention time to the retention time in the reference system. Another technique involves leveraging other experimental measurements of tubing pressure drops under various condition to develop a lookup table that can be used to identify likely tubing pressure drops in the target system. A third technique leverages knowledge of the separation procedure and the target system and the likely nature of the relevant flow to calculate tubing pressure drops in the target system.

公开(公告)号：US20180252684A1

公开(公告)日：2018-09-06

申请号：US15909264

申请日：2018-03-01

Applicant: Waters Technologies Corporation

Inventor： Michael O. Fogwill ,  Joseph A. Jarrell ,  Joseph D. Michienzi ,  Thomas S. McDonald

IPC: G01N30/32 ,  G01N30/34 ,  G01N30/72

CPC classification number: G01N30/32 ,  B01D15/163 ,  B01D15/40 ,  G01N30/34 ,  G01N30/36 ,  G01N30/62 ,  G01N30/7233 ,  G01N2030/326 ,  G01N2030/347 ,  G01N2030/623

Abstract: The present disclosure relates to methodologies, systems and apparatus for controlling pressure in a CO2-based chromatography system. A first CO2 pump operates in constant flow mode and delivers CO2 to a chromatography column, and liquid modifier is introduced to the chromatography column according to a gradient. A second CO2 pump is disposed downstream of the column and operates in constant pressure mode to introduce CO2 into a flow stream at an output of the column. Liquid modifier is also introduced into the flow stream at the output of the column according to a reverse gradient compared to the gradient entering the chromatography column.

公开(公告)号：US20180224404A1

公开(公告)日：2018-08-09

申请号：US15891463

申请日：2018-02-08

Applicant: Waters Technologies Corporation

Inventor： Michael O. Fogwill ,  Martin Gilar ,  Fabrice Gritti ,  Joseph D. Michienzi

IPC: G01N30/30

CPC classification number: G01N30/30 ,  G01N30/34 ,  G01N30/6095 ,  G01N2030/027 ,  G01N2030/3015 ,  G01N2030/3038 ,  G01N2030/3046 ,  G01N2030/3053

Abstract: A method of performing a chromatographic separation includes generating a spatial temperature gradient along a length of a chromatographic column in a liquid chromatography system. A sample is injected into a flow of a mobile phase to the column and a flow of a mobile phase having a composition gradient is provided to the column after the sample is received at the column. The spatial temperature gradient is moved along the length of the column from the column inlet to the column outlet during the time that the composition gradient traverses the column. This coordination of the composition gradient with the movement of the spatial thermal gradient yields a significant increase in peak capacity per unit time compared with conventional separation techniques performed in a conventional isothermal column environment.