公开(公告)号：US20070077595A1

公开(公告)日：2007-04-05

申请号：US11239100

申请日：2005-09-30

申请人： Tae-Woong Koo ,  Richard Jones ,  Andrew Berlin

发明人： Tae-Woong Koo ,  Richard Jones ,  Andrew Berlin

IPC分类号： G01N33/53 ,  G01J3/45 ,  G01B9/02

CPC分类号： G01J3/02 ,  B01J2219/00572 ,  B01J2219/00659 ,  B01J2219/00704 ,  B82Y30/00 ,  G01J3/0218 ,  G01J3/0259 ,  G01J3/44 ,  G01J3/4531 ,  G01J3/4532 ,  G01N21/658 ,  G02B6/12007

摘要： One embodiment relates to an analyzer having an interferometer, a detector and a microprocessor, wherein the analyzer does not contain a spectrometer having a dispersive grating, the interferometer is to create a phase shift in an original spectrum of electromagnetic radiation emitted from a sample and Fourier transform the original spectrum to a Fourier transform spectrum, the detector is to detect a characteristic of the Fourier transform spectrum, and the microprocessor comprises software or a hardware to inverse transform the Fourier transform spectrum and reproduce the original spectrum. Another embodiment relates to a Raman analyzer having an interferometer, wherein the Raman analyzer contains no dispersive grating or moving parts and has an ability to analyze a Raman signal. The embodiments of the invention could be used for analyzing a sample by striking a laser to the sample and examining the spectrum of the emitted electromagnetic radiation from the sample.

摘要翻译： 一个实施例涉及一种具有干涉仪，检测器和微处理器的分析仪，其中分析仪不包含具有分散光栅的光谱仪，干涉仪将在从样品和傅里叶发射的电磁辐射的原始光谱中产生相移 将原始频谱转换为傅里叶变换频谱，检测器检测傅里叶变换频谱的特性，微处理器包括软件或硬件，对傅立叶变换频谱进行逆变换，再现原始频谱。 另一个实施方案涉及具有干涉仪的拉曼分析仪，其中拉曼分析仪不含分散光栅或运动部件，并且具有分析拉曼信号的能力。 本发明的实施例可以用于通过将激光撞击到样品并检查来自样品的发射的电磁辐射的光谱来分析样品。

公开(公告)号：US20060216743A1

公开(公告)日：2006-09-28

申请号：US11443639

申请日：2006-05-30

申请人： Tae-Woong Koo ,  Andrew Berlin ,  Ken Salsman ,  Brian Ostrovsky

发明人： Tae-Woong Koo ,  Andrew Berlin ,  Ken Salsman ,  Brian Ostrovsky

IPC分类号： C12Q1/68 ,  G01N33/543

CPC分类号： G01N21/65 ,  B01J2219/00605 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00626 ,  B01J2219/00637 ,  B01J2219/00639 ,  B01J2219/00722 ,  B01L3/5027 ,  C12Q1/6825 ,  G01N21/3581 ,  G01N21/7743 ,  G01N33/54373 ,  G01N2021/651 ,  C12Q2545/114 ,  C12Q2523/313

摘要： Provided herein are methods and systems for detecting biomolecular binding events using gigahertz or terahertz radiation. The methods and systems use low-energy spectroscopy to detect biomolecular binding events between molecules in an aqueous solution. The detected biomolecular binding events include, for example, nucleic acid hybridizations, antibody/antigen binding, and receptor/ligand binding.

摘要翻译： 本文提供了使用千兆兹或太赫兹辐射检测生物分子结合事件的方法和系统。 该方法和系统使用低能谱来检测水溶液中分子之间的生物分子结合事件。 检测到的生物分子结合事件包括例如核酸杂交，抗体/抗原结合和受体/配体结合。

公开(公告)号：US07656523B2

公开(公告)日：2010-02-02

申请号：US11477379

申请日：2006-06-30

申请人： Lei Sun ,  Tae-Woong Koo ,  Liming Wang

发明人： Lei Sun ,  Tae-Woong Koo ,  Liming Wang

IPC分类号： G01J3/44

CPC分类号： G01N21/658 ,  G01J3/36 ,  G01J3/44

摘要： A device (and methods of using and manufacturing the device) that utilize a plurality of photomultipliers (PMT)s or a photodiodes coupled with a set of filters to collect Raman signal from samples. Also a method of detecting Raman signals includes receiving Raman signals from a sample utilizing a plurality of photomultiplier tubes (PMT)s or photodiodes, wherein at least one PMT or photodiode provides a different Raman signal than at least one other PMT or photodiode.

摘要翻译： 利用多个光电倍增管（PMT）或与一组滤波器耦合的光电二极管以从样品收集拉曼信号的装置（以及使用和制造该装置的方法）。 检测拉曼信号的方法还包括利用多个光电倍增管（PMT）或光电二极管从样品接收拉曼信号，其中至少一个PMT或光电二极管提供与至少一个其它PMT或光电二极管不同的拉曼信号。

公开(公告)号：US08513002B2

公开(公告)日：2013-08-20

申请号：US13185712

申请日：2011-07-19

申请人： Andrew Arthur Berlin ,  Christopher Marc Gerth ,  Tae-Woong Koo

发明人： Andrew Arthur Berlin ,  Christopher Marc Gerth ,  Tae-Woong Koo

IPC分类号： C12M1/34 ,  G01N33/00 ,  G01N33/553 ,  C12Q1/68 ,  C12M3/00

CPC分类号： G01N21/65 ,  G01N21/658 ,  G01N2021/052 ,  G01N2021/653 ,  G01N2021/655 ,  Y10T436/143333

摘要： Spectroscopic analysis systems and methods for analyzing samples are disclosed. An analysis system may contain an electromagnetic radiation source to provide radiation, a spectroscopic analysis chamber to perform a coherent Raman spectroscopy (e.g., stimulated Raman or coherent anti-Stokes Raman spectroscopy), and a radiation detector to detect radiation based on the spectroscopy. The chamber may have a resonant cavity to contain a sample for analysis, at least one window to the cavity to transmit the first radiation into the cavity and to transmit a second radiation out, a plurality of reflectors affixed to a housing of the cavity to reflect radiation of a predetermined frequency, the plurality of reflectors separated by a distance that is sufficient to resonate the radiation. The spectroscopic analysis system may be coupled with a nucleic acid sequencing system to receive a single nucleic acid derivative in solution and identify the derivative to sequence the nucleic acid.

摘要翻译： 公开了用于分析样品的光谱分析系统和方法。 分析系统可以包含用于提供辐射的电磁辐射源，用于执行相干拉曼光谱（例如，受激拉曼或相干反斯托克斯拉曼光谱）的光谱分析室，以及基于光谱检测辐射的辐射检测器。 腔室可以具有一个谐振腔，以容纳用于分析的样本，至少一个窗口，用于将腔室中的第一辐射传输到腔体中并将第二辐射透射出来，多个反射器固定到空腔的壳体以反射 预定频率的辐射，多个反射器分开足以使辐射共振的距离。 光谱分析系统可以与核酸测序系统结合以在溶液中接受单个核酸衍生物，并鉴定衍生物以对核酸进行排序。

公开(公告)号：US20120034686A1

公开(公告)日：2012-02-09

申请号：US13220214

申请日：2011-08-29

申请人： Andrew Arthur Berlin ,  Christopher Marc Gerth ,  Tae-Woong Koo

发明人： Andrew Arthur Berlin ,  Christopher Marc Gerth ,  Tae-Woong Koo

IPC分类号： C12M1/34

CPC分类号： G01N21/65 ,  G01N21/658 ,  G01N2021/052 ,  G01N2021/653 ,  G01N2021/655 ,  Y10T436/143333

摘要： Spectroscopic analysis systems and methods for analyzing samples are disclosed. An analysis system may contain an electromagnetic radiation source to provide radiation, a spectroscopic analysis chamber to perform a coherent Raman spectroscopy (e.g., stimulated Raman or coherent anti-Stokes Raman spectroscopy), and a radiation detector to detect radiation based on the spectroscopy. The chamber may have a resonant cavity to contain a sample for analysis, at least one window to the cavity to transmit the first radiation into the cavity and to transmit a second radiation out, a plurality of reflectors affixed to a housing of the cavity to reflect radiation of a predetermined frequency, the plurality of reflectors separated by a distance that is sufficient to resonate the radiation. The spectroscopic analysis system may be coupled with a nucleic acid sequencing system to receive a single nucleic acid derivative in solution and identify the derivative to sequence the nucleic acid.

摘要翻译： 公开了用于分析样品的光谱分析系统和方法。 分析系统可以包含用于提供辐射的电磁辐射源，用于执行相干拉曼光谱（例如，受激拉曼或相干反斯托克斯拉曼光谱）的光谱分析室，以及基于光谱检测辐射的辐射检测器。 腔室可以具有一个谐振腔，以容纳用于分析的样本，至少一个窗口，用于将腔室中的第一辐射传输到腔体中并将第二辐射透射出来，多个反射器固定到空腔的壳体以反射 预定频率的辐射，多个反射器分开足以使辐射共振的距离。 光谱分析系统可以与核酸测序系统结合以在溶液中接受单个核酸衍生物，并鉴定衍生物以对核酸进行排序。

公开(公告)号：US20070154881A1

公开(公告)日：2007-07-05

申请号：US11319791

申请日：2005-12-29

申请人： Tae-Woong Koo

发明人： Tae-Woong Koo

IPC分类号： C12Q1/70

CPC分类号： B82Y15/00 ,  B82Y30/00 ,  C12Q1/6823 ,  C12Q2565/629 ,  C12Q2565/501

摘要： The application relates to a method, system and device for performing biological assays. The method, system and device allow more accurate and specific detections of biomolecules in multiplex assays, such as immunoassays and DNA microarray assays. More specifically, the embodiments of the invention allow the detection of labels after their detachment or disassociation from a binding situation wherein interference from other labels or the background may reduce the accuracy of specificity of the detection. The embodiments of the invention further allow detection of individual labels.

摘要翻译： 本申请涉及用于进行生物测定的方法，系统和装置。 该方法，系统和装置允许在多重测定（例如免疫测定和DNA微阵列测定）中更准确和更具体地检测生物分子。 更具体地说，本发明的实施例允许在其与其他标签或背景的干扰可能降低检测的特异性的准确性的结合情况下分离或分离之后检测标签。 本发明的实施例还允许检测单个标签。

公开(公告)号：US20060181702A1

公开(公告)日：2006-08-17

申请号：US11388345

申请日：2006-03-24

申请人： Tae-Woong Koo ,  Mineo Yamakawa

发明人： Tae-Woong Koo ,  Mineo Yamakawa

IPC分类号： G01J3/44 ,  G01N21/65

CPC分类号： G01N21/65

摘要： Disclosed herein are a Raman spectroscopy structure comprising a porous material substrate, and a method of performing Raman spectroscopy of a sample disposed adjacent to the structure comprising the porous material substrate. Generally, the substrate includes one or more layers of a porous material such as porous silicon, porous polysilicon, porous ceramics, porous silica, porous alumina, porous silicon-germanium, porous germanium, porous gallium arsenide, porous gallium phosphide, porous zinc oxide, and porous silicon carbide. It has been discovered that such a substrate material, when excited with near-infrared light, does not exhibit undesired background fluorescence characteristic of other known Raman spectroscopy substrates.

公开(公告)号：US07518728B2

公开(公告)日：2009-04-14

申请号：US11239118

申请日：2005-09-30

申请人： Tae-Woong Koo

发明人： Tae-Woong Koo

IPC分类号： G01B9/02 ,  G01J3/45

CPC分类号： G01J3/44 ,  G01J3/453 ,  G01N21/253 ,  G01N21/65 ,  G01N21/658

摘要： An instrument having an illumination source configured to illuminate a field of illumination on a surface of a substrate that is configured to hold a sample. The field of illumination typically has a diameter greater than 1 micron or an area greater than that of at least one pad of an array. The instrument also includes an interferometer, and a detectors. The instrument is configured to perform Fourier transform imaging without single spot scanning or without line scanning. Additionally, the instrument may include an illumination light source, an array detector and spectral processing electronics. A method of collecting Fourier transform (FT) data is also disclosed.

摘要翻译： 具有照明源的器械，被配置为照亮被配置为保持样品的衬底的表面上的照明场。 照明场通常具有大于1微米的直径或大于阵列的至少一个焊盘的面积。 该仪器还包括一个干涉仪和一个探测器。 该仪器被配置为执行傅里叶变换成像，无需单点扫描或无线扫描。 此外，仪器可以包括照明光源，阵列检测器和光谱处理电子器件。 还公开了一种收集傅里叶变换（FT）数据的方法。

公开(公告)号：US07361501B2

公开(公告)日：2008-04-22

申请号：US11239100

申请日：2005-09-30

申请人： Tae-Woong Koo ,  Richard Jones ,  Andrew A. Berlin

发明人： Tae-Woong Koo ,  Richard Jones ,  Andrew A. Berlin

IPC分类号： C12M3/00

CPC分类号： G01J3/02 ,  B01J2219/00572 ,  B01J2219/00659 ,  B01J2219/00704 ,  B82Y30/00 ,  G01J3/0218 ,  G01J3/0259 ,  G01J3/44 ,  G01J3/4531 ,  G01J3/4532 ,  G01N21/658 ,  G02B6/12007

摘要： One embodiment relates to an analyzer having an interferometer, a detector and a microprocessor, wherein the analyzer does not contain a spectrometer having a dispersive grating, the interferometer is to create a phase shift in an original spectrum of electromagnetic radiation emitted from a sample and Fourier transform the original spectrum to a Fourier transform spectrum, the detector is to detect a characteristic of the Fourier transform spectrum, and the microprocessor comprises software or a hardware to inverse transform the Fourier transform spectrum and reproduce the original spectrum. Another embodiment relates to a Raman analyzer having an interferometer, wherein the Raman analyzer contains no dispersive grating or moving parts and has an ability to analyze a Raman signal. The embodiments of the invention could be used for analyzing a sample by striking a laser to the sample and examining the spectrum of the emitted electromagnetic radiation from the sample.

摘要翻译： 一个实施例涉及一种具有干涉仪，检测器和微处理器的分析仪，其中分析仪不包含具有分散光栅的光谱仪，干涉仪将在从样品和傅里叶发射的电磁辐射的原始光谱中产生相移 将原始频谱转换为傅里叶变换频谱，检测器检测傅里叶变换频谱的特性，微处理器包括软件或硬件，对傅立叶变换频谱进行逆变换，再现原始频谱。 另一个实施方案涉及具有干涉仪的拉曼分析仪，其中拉曼分析仪不含分散光栅或运动部件，并且具有分析拉曼信号的能力。 本发明的实施例可以用于通过将激光撞击到样品并检查来自样品的发射的电磁辐射的光谱来分析样品。

公开(公告)号：US20050110990A1

公开(公告)日：2005-05-26

申请号：US10966893

申请日：2004-10-15

申请人： Tae-Woong Koo ,  Christopher Gerth ,  Mineo Yamakawa

发明人： Tae-Woong Koo ,  Christopher Gerth ,  Mineo Yamakawa

IPC分类号： G01J3/44 ,  G01N21/65

CPC分类号： G01J3/44 ,  G01N21/658 ,  G01N2021/653 ,  G01N2021/656

摘要： The device and method disclosed herein concern detecting, identifying, and or quantifying analytes, such as nucleic acids, with high resolution and fast response times using surface enhanced coherent anti-Stokes Raman spectroscopy. In certain embodiments of the invention, a small number molecular sample of the analyte 210 such as a nucleotide, passes through a microfluidic channel, microchannel, or nanochannel 185 and sample cell 175 that contains Raman-active surfaces, and is detected by surface enhanced, coherent anti-Stokes Raman spectroscopy (SECARS). Other embodiments of the invention concern an apparatus for analyte detection.

摘要翻译： 本文公开的装置和方法涉及使用表面增强的相干反斯托克斯拉曼光谱法检测，鉴定和定量分析物，例如具有高分辨率和快速响应时间的核酸。 在本发明的某些实施方案中，诸如核苷酸的分析物210的少量分子样品通过含有拉曼活性表面的微流体通道，微通道或纳米通道185和样品池175，并且通过表面增强， 相干反斯托克斯拉曼光谱（SECARS）。 本发明的其它实施方案涉及用于分析物检测的装置。