公开(公告)号：US20150126633A1

公开(公告)日：2015-05-07

申请号：US14530941

申请日：2014-11-03

Applicant: INDUSTRY-ACADEMIC COOPERATION FOUNDATION, YONSEI UNIVERSITY

Inventor： Hyung Il JUNG ,  Cheng Guo LI

IPC: D01D5/00 ,  C08J9/26 ,  G01N27/28 ,  C08J9/00

CPC classification number: D01D5/0007 ,  A61M2037/0053 ,  B33Y80/00 ,  C08J9/0061 ,  C08J9/0085 ,  C08J9/26 ,  C08J2201/042 ,  C08J2367/04 ,  C08J2439/06 ,  D01D5/0076 ,  D01F1/10 ,  D10B2331/041 ,  G01N27/28 ,  G01N27/3278

Abstract: The present invention relates to a method for fabricating a three-dimensional porous fibrous microstructure, various three-dimensional porous fibrous microstructures fabricated by the method, an apparatus for detecting a biological marker and a drug delivery system comprising the microstructure. The porous fibrous microstructure of the present invention has excellent interconnectivity between pores and micropores and captures and delivers target particles at high efficiency, and thus can be usefully applied to biomedical applications including the detection of a biomarker and drug delivery.

Abstract translation: 本发明涉及一种制造三维多孔纤维微结构的方法，通过该方法制造的各种三维多孔纤维微结构，用于检测生物标志物的装置和包含微结构的药物递送系统。 本发明的多孔纤维显微组织具有优异的孔隙和微孔相互连接性，并以高效率捕获并输送目标颗粒，因此可用于生物医学应用，包括检测生物标志物和药物递送。

公开(公告)号：US08926813B2

公开(公告)日：2015-01-06

申请号：US13567595

申请日：2012-08-06

Applicant: John S. Oliver

Inventor： John S. Oliver

IPC: C07K1/26 ,  G01N33/487 ,  G01N27/327

CPC classification number: G01N33/48721 ,  G01N27/3278 ,  G01N27/4473 ,  Y10S977/957

Abstract: Devices and methods for detecting the length of analytes, and/or sequencing analytes are provided in which two or more electrical signals are obtained as an analyte traverses a fluidic channel. Detection of the relative position of probes hybridized to a biopolymer and/or the length of the analyte (e.g., a biopolymer) does not rely on the absolute time between detection events of a given electrical signal to determine a distance associated with the biopolymer. Instead, multiple signals are obtained as functions of time) corresponding to a plurality of detector volumes at known locations along a fluidic channel through which the biopolymer passes, and the distances are determined from the multiple signals.

Abstract translation: 提供了用于检测分析物的长度和/或测序分析物的装置和方法，其中当分析物穿过流体通道时获得两个或多个电信号。 检测与生物聚合物杂交的探针和/或分析物（例如，生物聚合物）的长度的相对位置不依赖于给定电信号的检测事件之间的绝对时间来确定与生物聚合物相关联的距离。 相反，多个信号作为时间的函数获得），其对应于沿着生物聚合物通过的流体通道的已知位置处的多个检测器体积，并且距离由多个信号确定。

公开(公告)号：US20140255918A1

公开(公告)日：2014-09-11

申请号：US14192785

申请日：2014-02-27

Applicant: The Regents of the University of California

Inventor： Felix A. Olasagasti ,  Kathy R. Lieberman ,  Seico Benner ,  Mark A. Akeson

IPC: C12Q1/68

CPC classification number: C12Q1/6874 ,  C12Q1/54 ,  C12Q1/6869 ,  C25B3/10 ,  G01N27/3278 ,  G01N27/4166 ,  G01N33/48721 ,  C12Q2522/101 ,  C12Q2565/631

Abstract: The invention herein disclosed provides for devices and methods that can detect and control an individual polymer in a mixture is acted upon by another compound, for example, an enzyme, in a nanopore in the absence of requiring a terminating nucleotide. The devices and methods are also used to determine rapidly (˜>50 Hz) the nucleotide base sequence of a polynucleotide under feedback control or using signals generated by the interactions between the polynucleotide and the nanopore. The invention is of particular use in the fields of drug discovery, molecular biology, structural biology, cell biology, molecular switches, molecular circuits, and molecular computational devices, and the manufacture thereof.

Abstract translation: 本文公开的本发明提供了可以检测和控制混合物中的单个聚合物在不需要终止核苷酸的情况下在纳米孔中被另一种化合物（例如酶）作用的装置和方法。 所述装置和方法还用于在反馈控制下或使用通过多核苷酸和纳米孔之间的相互作用产生的信号来快速（〜> 50Hz）确定多核苷酸的核苷酸碱基序列。 本发明特别用于药物发现，分子生物学，结构生物学，细胞生物学，分子开关，分子电路和分子计算装置及其制造领域。

公开(公告)号：US20140034517A1

公开(公告)日：2014-02-06

申请号：US14056636

申请日：2013-10-17

Applicant: The Regents of the University of California

Inventor： Mark A. Akeson ,  David W. Deamer ,  William B. Dunbar ,  Roger Jinteh Arrigo Chen ,  Noah A. Wilson

IPC: G01N33/487

CPC classification number: C12Q1/6874 ,  C12Q1/54 ,  C12Q1/6869 ,  C25B3/10 ,  G01N27/3278 ,  G01N27/4166 ,  G01N33/48721 ,  C12Q2522/101 ,  C12Q2565/631

Abstract: Devices and methods that can detect and control an individual polymer in a mixture is acted upon by another compound, for example, an enzyme, in a nanopore are provided. The devices and methods also determine (˜>50 Hz) the nucleotide base sequence of a polynucleotide under feedback control or using signals generated by the interactions between the polynucleotide and the nanopore. The invention is of particular use in the fields of molecular biology, structural biology, cell biology, molecular switches, molecular circuits, and molecular computational devices, and the manufacture thereof.

Abstract translation: 可以检测和控制混合物中单个聚合物的装置和方法被提供于另一种化合物，例如纳米孔中的酶。 所述装置和方法还在反馈控制下或使用由多核苷酸和纳米孔之间的相互作用产生的信号来确定多核苷酸的核苷酸碱基序列（〜> 50Hz）。 本发明特别用于分子生物学，结构生物学，细胞生物学，分子开关，分子电路和分子计算装置及其制造领域。

公开(公告)号：US20140002111A1

公开(公告)日：2014-01-02

申请号：US13538570

申请日：2012-06-29

Applicant: Radislav Alexandrovich Potyrailo ,  Cheryl Margaret Surman ,  Steven Yuehin Go ,  Yongjae Lee

Inventor： Radislav Alexandrovich Potyrailo ,  Cheryl Margaret Surman ,  Steven Yuehin Go ,  Yongjae Lee

IPC: G01N27/02 ,  H05K3/30

CPC classification number: H05K1/16 ,  G01N27/026 ,  G01N27/3278 ,  G01N33/0031 ,  G01N33/48792 ,  H05K2201/0338 ,  H05K2201/09263 ,  H05K2201/10151 ,  Y10T29/4913

Abstract: A sensing system includes an inductor-capacitor-resistor (LCR) resonator sensor having a substrate, a plurality of first sensing elements mutually spaced apart and disposed on the substrate, and a sensing material film being disposed on a first sensing region of the corresponding first sensing element.

Abstract translation: 感测系统包括具有衬底的电感器 - 电容 - 电阻（LCR）谐振器传感器，多个相互间隔开并设置在衬底上的第一感测元件，感测材料膜设置在相应的第一感测区域的第一感测区域上 传感元件。

公开(公告)号：US20140001055A1

公开(公告)日：2014-01-02

申请号：US13538346

申请日：2012-06-29

Applicant: Oguz H. Elibol ,  Grace M. Credo ,  Xing Su ,  Madoo Varma ,  Jonathan S. Daniels ,  Drew Hall ,  Handong Li ,  Noureddine Tayebi ,  Kai Wu

Inventor： Oguz H. Elibol ,  Grace M. Credo ,  Xing Su ,  Madoo Varma ,  Jonathan S. Daniels ,  Drew Hall ,  Handong Li ,  Noureddine Tayebi ,  Kai Wu

IPC: G01N27/26 ,  B82Y5/00 ,  B82Y30/00

CPC classification number: G01N27/3277 ,  B01J19/0046 ,  B01J2219/00608 ,  B01J2219/00653 ,  B01J2219/00722 ,  B81B7/04 ,  B81B2201/0214 ,  B81B2201/058 ,  B82Y15/00 ,  C40B20/04 ,  C40B50/16 ,  G01N27/3278 ,  G01N27/4145 ,  G01N27/4146 ,  G01N27/4148

Abstract: Various embodiments provide devices, methods, and systems for high throughput biomolecule detection using transducer arrays. In one embodiment, a transducer array made up of transducer elements may be used to detect byproducts from chemical reactions that involve redox genic tags. Each transducer element may include at least a reaction chamber and a fingerprinting region, configured to flow a fluid from the reaction chamber through the fingerprinting region. The reaction chamber can include a molecule attachment region and the fingerprinting region can include at least one set of electrodes separated by a nanogap for conducting redox cycling reactions. In embodiments, by flowing the chamber content obtained from a reaction of a latent redox tagged probe molecule, a catalyst, and a target molecule in the reaction chamber through the fingerprinting region, the redox cycling reactions can be detected to identify redox-tagged biomolecules.

Abstract translation: 各种实施方案提供使用换能器阵列进行高通量生物分子检测的装置，方法和系统。 在一个实施例中，由换能器元件组成的换能器阵列可用于检测涉及氧化还原基因标签的化学反应的副产物。 每个换能器元件可以至少包括反应室和指纹区域，其被配置为使来自反应室的流体通过指纹区域流动。 反应室可以包括分子附着区域，并且指纹区域可以包括由纳米间隙分开的至少一组电极，用于进行氧化还原循环反应。 在实施方案中，通过使反应室中潜氧氧化还原标记的探针分子，催化剂和靶分子的反应通过指纹区域流动所述腔室内容物，可以检测氧化还原循环反应以鉴定氧化还原标记的生物分子。

公开(公告)号：US20130331299A1

公开(公告)日：2013-12-12

申请号：US14001927

申请日：2012-02-27

Applicant: Torsten Reda ,  Jakob Haglmueller ,  Georg Schitter ,  Alexander Seitz

Inventor： Torsten Reda ,  Jakob Haglmueller ,  Georg Schitter ,  Alexander Seitz

IPC: C12Q1/68

CPC classification number: C12Q1/686 ,  C12Q1/6837 ,  G01N27/3278

Abstract: The present invention provides a sensor array device with multiple sensor junctions which have been created through the assembly of two or more differently functionalized surfaces. The functionalizing of the prospective sensor junction areas with sensor compounds occurred when the different surfaces were physically separated from each other before the assembly of the sensor array. By these means, sensor junctions can be built smaller than conventional deposition techniques like printing and photolithography would allow for otherwise. As a consequence, each individual sensor junction contains two potentially different sensor compounds. The sensor array identifies and quantifies different biomolecules.

Abstract translation: 本发明提供一种具有多个传感器接头的传感器阵列装置，其通过组装两个或多个不同功能化的表面而产生。 在组装传感器阵列之前，当不同的表面彼此物理分离时，发生具有传感器化合物的预期传感器接合区域的功能化。 通过这些方式，可以将传感器结形成为比常规沉积技术更小，如印刷和光刻将允许其他方式。 因此，每个传感器接头都包含两种潜在的传感器化合物。 传感器阵列识别和量化不同的生物分子。

公开(公告)号：US20130298394A1

公开(公告)日：2013-11-14

申请号：US13943753

申请日：2013-07-16

Applicant: HON HAI PRECISION INDUSTRY CO., LTD. ,  Tsinghua University

Inventor： XUE-SHEN WANG ,  QUN-QING LI ,  SHOU-SHAN FAN

IPC: G01N33/50

CPC classification number: G01N33/50 ,  B05D5/12 ,  B82Y15/00 ,  B82Y30/00 ,  C23C16/26 ,  G01N27/3275 ,  G01N27/3278 ,  Y10T29/49002 ,  Y10T29/49121

Abstract: A method for fabricating a plurality of biosensors includes the steps of: providing a base with a surface; forming a carbon nanotube array including a plurality of carbon nanotubes substantially parallels to each other on the surface; forming a plurality of lead pairs on the surface, the plurality of lead pairs divides the plurality of carbon nanotubes into a plurality of first carbon nanotubes and a plurality of second carbon nanotubes; eliminating the plurality of second carbon nanotubes; cutting the plurality of first carbon nanotubes to form a plurality of third carbon nanotubes and a plurality of fourth carbon nanotubes; and fabricating a plurality of receptors to electrically connect the plurality of third carbon nanotubes to the plurality of fourth carbon nanotubes.

Abstract translation: 制造多个生物传感器的方法包括以下步骤：提供具有表面的基底; 形成包括在表面上彼此基本平行的多个碳纳米管的碳纳米管阵列; 在所述表面上形成多个引线对，所述多个引线对将所述多个碳纳米管分成多个第一碳纳米管和多个第二碳纳米管; 消除多个第二碳纳米管; 切割多个第一碳纳米管以形成多个第三碳纳米管和多个第四碳纳米管; 以及制造多个受体以将多个第三碳纳米管电连接到多个第四碳纳米管。

公开(公告)号：US20130209807A1

公开(公告)日：2013-08-15

申请号：US13766385

申请日：2013-02-13

Applicant: Florida State University Research Foundation, Inc.

Inventor： Jhunu Chatterjee

IPC: H01J9/02

CPC classification number: H01J9/02 ,  B82Y15/00 ,  B82Y30/00 ,  G01N27/308 ,  G01N27/3278 ,  Y10T428/30

Abstract: Electrodes and methods for making electrodes including modified carbon nanotube sheets are provided. The carbon nanotube sheets can be modified with metal particles or at least one mediator titrant. The electrodes can be disposed on a glassy carbon electrode to modify the glassy carbon electrode. Methods are provided that include forming a suspension of carbon nanotubes and metal particles or at least one mediator titrant, and filtering the suspension to form a modified carbon nanotube sheet.

Abstract translation: 提供电极和用于制造包括改性碳纳米管片的电极的方法。 碳纳米管片可以用金属颗粒或至少一种介体滴定剂进行改性。 电极可以设置在玻璃碳电极上以改变玻璃碳电极。 提供了包括形成碳纳米管和金属颗粒或至少一种介体滴定剂的悬浮液的方法，并过滤悬浮液以形成改性的碳纳米管片。

公开(公告)号：US20130183243A1

公开(公告)日：2013-07-18

申请号：US13809387

申请日：2011-07-12

Applicant: Jeffrey LaBelle ,  Ugur Demirok

Inventor： Jeffrey LaBelle ,  Ugur Demirok

IPC: G01N27/02 ,  A61B5/0205 ,  G01N33/68

CPC classification number: G01N27/026 ,  A61B5/0205 ,  G01N27/3278 ,  G01N33/5438 ,  G01N33/6869

Abstract: The present invention relates to a diagnostic device and methods of using the same for diagnostic assays for monitoring the presence of biological samples wherein the device allows for the determination of at least two assay components on one sensor. More specifically, the invention relates to a multi-marker electrochemical impedance spectroscopy sensor comprising a plurality of molecular recognition elements wherein the sensor comprises multiple different molecular recognition element types that are tuned in a manner that alters the frequency of the molecular recognition element type such that it is at a detectably different frequency to the frequency of other molecular recognition element types on the same sensor.

Abstract translation: 本发明涉及一种诊断装置及其用于诊断测定用于监测生物样品的存在的方法，其中该装置允许在一个传感器上确定至少两个测定组分。 更具体地，本发明涉及包括多个分子识别元件的多标记电化学阻抗光谱传感器，其中传感器包括多个不同的分子识别元件类型，其以改变分子识别元件类型的频率的方式进行调谐，使得 与同一传感器上的其他分子识别元件类型的频率具有可检测的不同频率。