公开(公告)号：US10078206B2

公开(公告)日：2018-09-18

申请号：US15087177

申请日：2016-03-31

申请人： Carl Zeiss Microscopy GmbH

发明人： Thomas Kalkbrenner ,  Michael Goelles

IPC分类号： G02B21/36 ,  G02B21/00 ,  G01N21/64 ,  G02B21/18 ,  G02B17/02 ,  G02B21/16 ,  G02B27/10 ,  G02B27/14 ,  G02B27/58 ,  G02B7/38 ,  G02B21/24

CPC分类号： G02B21/361 ,  G01N21/6458 ,  G02B7/38 ,  G02B17/023 ,  G02B21/0032 ,  G02B21/0072 ,  G02B21/0076 ,  G02B21/0088 ,  G02B21/16 ,  G02B21/18 ,  G02B21/245 ,  G02B21/367 ,  G02B27/106 ,  G02B27/1066 ,  G02B27/141 ,  G02B27/144 ,  G02B27/145 ,  G02B27/58

摘要： The invention relates to a microscope having an illumination beam path with wide field illumination of a sample and a first detection beam path having a spatially resolved surface receiver, which is reached by a first part of the detection light coming from the sample via the first detection beam path, or an image divider assembly for a microscope. In order to lengthen the optical path length, at least a second part of the detection light coming from the sample is masked out of the detection beam path and, via deflection means belonging to the detection beam path, is led into a second detection beam path and, preferably via further deflection means, is deflected back in the direction of the detection in such a way that detection light is applied to at least two partial regions beside one another on the surface receiver. At least the second part of the detection light runs in an optical element having an optical density that is increased as compared with the first detection beam path, in order to lengthen the optical path length, and the optical element is designed to be displaceable at an angle, preferably perpendicular, to the optical axis of the first detection beam path in order to adjust the optical path length, and has flat surfaces, at least on the light entry and light exit side thereof; a prism is provided, preferably a glass prism, preferably at least in the second detection beam path after a first beam deflection, for deflection in a direction parallel to the first detection beam path, in order to increase the path length and for reverse deflection.

公开(公告)号：US10018825B2

公开(公告)日：2018-07-10

申请号：US14870850

申请日：2015-09-30

申请人： Chung Yuan Christian University ,  Vivi3D Technology Inc.

发明人： Yih-Chih Hsu ,  Cyun-Fang Hsu ,  Jau-Jiu Ju ,  Chi-Lung Chang

IPC分类号： G02B7/09 ,  G03B3/10 ,  G03B13/36 ,  H02K33/16 ,  G02B21/36 ,  G02B21/26 ,  G02B21/06 ,  G02B21/00 ,  H04N21/239 ,  H04N21/2743 ,  H04N21/61

CPC分类号： G02B21/367 ,  G02B21/0008 ,  G02B21/0088 ,  G02B21/086 ,  G02B21/362 ,  H04N21/2393 ,  H04N21/2743 ,  H04N21/6125

摘要： A microscope monitoring device includes a pedestal, a flat light source, a microscope set, an image sensor, a motion stage and a controlling circuit board. The motion stage carries the microscope set and the image sensor and moves them according to a driving signal. Responding to a trigger signal, the image sensor senses the light passing through one or more culture tank on the multiple-well plate and the microscope set so as to generate an image signal. The controlling circuit board outputs the trigger signal and the driving signal to obtain an image of cells in the multiple-well plate. A microscope monitoring system including a server and the aforementioned microscope monitoring device is also provided.

公开(公告)号：US20180129028A1

公开(公告)日：2018-05-10

申请号：US15790631

申请日：2017-10-23

申请人： FUJIFILM Corporation

发明人： Takashi MUROOKA

IPC分类号： G02B21/02 ,  C12M1/34 ,  G01N33/50

CPC分类号： G02B21/025 ,  C12M41/36 ,  C12M41/46 ,  C12M41/48 ,  G01N33/5073 ,  G02B21/0032 ,  G02B21/0088 ,  G02B21/088

摘要： An imaging apparatus includes: an imaging apparatus main body for imaging an observation target contained in a container; and a liquid droplet adhesion determination unit that acquires pattern information of liquid droplets adhering to a bottom surface on an outer side of the container based on an image of the observation target imaged by the imaging apparatus main body and determines whether or not the liquid droplets adhere to the bottom surface based on the pattern information. In a case where the liquid droplet adhesion determination unit determines that the liquid droplets adhere to the bottom surface on the outer side of the container, the imaging apparatus main body images the observation target again after performing liquid droplet removal processing.

公开(公告)号：US09915919B2

公开(公告)日：2018-03-13

申请号：US15033608

申请日：2014-10-27

申请人： UNIVERSITAT DE VALENCIA ,  UNIVERSIDAD NACIONAL DE COLUMBIA

发明人： Emilio Sánchez Ortiga ,  Manuel Martínez Corral ,  Ana Doblas Expósito ,  Genaro Saavedra Tortosa ,  Jorge Iván García Sucerquia

IPC分类号： G03H1/00 ,  G02B21/00 ,  G02B21/14 ,  G02B21/36 ,  G03H1/04 ,  G02B13/00 ,  G02B13/22 ,  G02B21/04 ,  G02B27/30 ,  G03H1/16

CPC分类号： G03H1/0005 ,  G02B13/0095 ,  G02B13/22 ,  G02B21/0088 ,  G02B21/04 ,  G02B21/14 ,  G02B21/36 ,  G02B21/367 ,  G02B27/30 ,  G03H1/041 ,  G03H1/0443 ,  G03H1/16 ,  G03H2001/005 ,  G03H2001/0452 ,  G03H2001/0456

摘要： A microscope, method and computer program for obtaining quantitative phase images by digital holographic microscopy. The microscope includes: a coherent light source (1) and a beam splitter (3) for generating an object beam (Lo) for illuminating a sample, and a reference beam (Lr); an optical system with a main optical path making up a telecentric afocal system, and a reference optical path; and recording means (12) recording a hologram of said sample in the image plane of the optical system. The method includes recording a hologram in the image plane of an optical telecentric afocal system. The computer program is adapted for implementing part of the steps of the method.

公开(公告)号：US09880378B2

公开(公告)日：2018-01-30

申请号：US14781625

申请日：2014-04-03

申请人： RIKEN

发明人： Yuichi Taniguchi ,  Kazuya Nishimura

IPC分类号： G02B21/16 ,  G02B21/08 ,  G02B21/33 ,  G02B21/26 ,  G02B21/00 ,  G02B21/24 ,  G01N21/64 ,  G02B21/10

CPC分类号： G02B21/16 ,  G01N21/6458 ,  G02B21/0076 ,  G02B21/0088 ,  G02B21/08 ,  G02B21/10 ,  G02B21/24 ,  G02B21/26 ,  G02B21/33

摘要： A microscope includes: a sample placement part having a placement surface on which to place a sample and a bottom face opposite to the placement surface; an observation lens; and an optical unit for generating sheet light and use of the microscope. The microscope of an embodiment is arranged such that the sheet light enters the sample placement part from the bottom face and passing through the sample placement part to irradiate the sample, and fluorescence from the sample passes through the sample placement part toward the bottom face to be received by the observation lens. The microscope can, with this arrangement, utilize the advantages of a SPIM, and allows observation of a sample which observation is free from a restriction of the size of a sample.

公开(公告)号：US20170285315A1

公开(公告)日：2017-10-05

申请号：US15436742

申请日：2017-02-17

申请人： OLYMPUS CORPORATION

发明人： Yusuke AMANO ,  Susumu HONDA

IPC分类号： G02B21/00 ,  G02B21/26 ,  G02B21/08

CPC分类号： G02B21/002 ,  G02B5/003 ,  G02B21/0088 ,  G02B21/08 ,  G02B21/16 ,  G02B21/26 ,  G02B27/0018

摘要： Provided is a laser scanning microscope including a stage on which a sample is placed, an objective lens that is disposed below the stage and that focuses laser light from a light source onto the sample, a scanner that scans the laser light focused by the objective lens over the sample, a condenser lens disposed opposite the objective lens with the stage interposed therebetween, and a light blocking cover that is disposed in an optical path between the condenser lens and the stage and that blocks external light entering the objective lens or the condenser lens from above the sample via the stage.

公开(公告)号：US09772483B2

公开(公告)日：2017-09-26

申请号：US14481956

申请日：2014-09-10

申请人： Keyence Corporation

发明人： Masaki Fujiwara

IPC分类号： G02B21/06 ,  G02B21/00 ,  G02B21/12 ,  G02B21/24 ,  G02B21/36 ,  G02B21/08

CPC分类号： G02B21/06 ,  G02B21/0088 ,  G02B21/086 ,  G02B21/12 ,  G02B21/24 ,  G02B21/361 ,  G02B21/365

摘要： To provide a microscopic imaging device capable of easily switching the imaging method. During sectioning observation and normal observation, a measuring object is irradiated with pattern measurement light and uniform measurement light generated by a light modulation element, respectively. The measuring object is irradiated with the pattern measurement light and the uniform measurement light through a common light path. During the sectioning observation, a spatial phase of the pattern is sequentially moved on the measuring object by a predetermined amount by the light modulation element, and sectioning image data indicating an image of the measuring object is generated based on a plurality of pieces of image data generated at a plurality of phases of the pattern based on the light receiving signal. During the normal observation, normal image data indicating an image of the measuring object is generated based on the light receiving signal.

公开(公告)号：US09578220B2

公开(公告)日：2017-02-21

申请号：US14713755

申请日：2015-05-15

申请人： PANASONIC HEALTHCARE HOLDINGS CO., LTD.

发明人： Mikio Houjou ,  Atsushi Oda

IPC分类号： G01N21/01 ,  G02B21/00 ,  G02B21/36 ,  C12M1/34 ,  H04N5/225 ,  G01N21/13 ,  A61B5/00

CPC分类号： H04N5/2254 ,  A61B5/0033 ,  C12M41/36 ,  G01N21/01 ,  G01N21/13 ,  G01N2201/022 ,  G01N2201/062 ,  G01N2201/0638 ,  G02B21/0088 ,  G02B21/361 ,  G02B21/365 ,  H04N5/2256

摘要： An observation device (1) is provided with: a general observation unit (10) for observing sample cells by observing an entire container (C) containing the cells and a culture solution; and a magnification observation unit (20) for magnifying a region within the container (C) and observing the cells, the general observation unit (10) and the magnification observation unit (20) each individually having lighting for illuminating the cells with light, and an optical system for observing the cells. The general observation unit (10) and the magnification observation unit (20) are thereby each provided with an individual optical system and lighting, making it possible to configure an appropriate observation unit for use both when the cells are observed by observing the entire container (C) and when a part within the container (C) is magnified and the cells are observed.

摘要翻译： 观察装置（1）具备：通过观察包含细胞的整个容器（C）和培养液观察样品池的一般观察单元（10） 以及用于放大容器（C）内的区域并观察细胞的放大观察单元（20），一般观察单元（10）和放大观察单元（20）各自具有用光照亮细胞的照明，以及 用于观察细胞的光学系统。 一般观察单元（10）和放大率观察单元（20）各自设置有单独的光学系统和照明，使得可以配置在通过观察整个容器观察细胞时使用的适当的观察单元（ C），并且当容器（C）中的一部分被放大并观察细胞时。

公开(公告)号：US20170045725A1

公开(公告)日：2017-02-16

申请号：US15226285

申请日：2016-08-02

申请人： OLYMPUS CORPORATION

发明人： Bunryu ARASHI

IPC分类号： G02B21/08 ,  G02B21/02

CPC分类号： G02B21/086 ,  G02B21/0032 ,  G02B21/0088 ,  G02B21/02

摘要： A microscope includes a condenser lens that is arranged removably and insertably with respect to a light path of light from a light source, and an objective that collects the light from the light source, which has been transmitted through a sample. A conditional expression below is satisfied when b is an exit pupil diameter of the objective and adet is a diameter of an area in a state in which the condenser lens has been removed from the light path, the area being included in an exit pupil plane of the objective and being an area through which the light from the light source, which has entered the objective through one point on the sample, passes: 1/20≦adet/b≦1/2   (1).

公开(公告)号：US20170031149A1

公开(公告)日：2017-02-02

申请号：US15293177

申请日：2016-10-13

申请人： Robert Levin ,  Brian Rasnow ,  Mel Shelhlein ,  Chris Shumate ,  Walter D. Niles

发明人： Robert Levin ,  Brian Rasnow ,  Mel Shelhlein ,  Chris Shumate ,  Walter D. Niles

IPC分类号： G02B21/16 ,  G02B21/26 ,  G02B21/36 ,  G02B21/00 ,  G02B21/12

CPC分类号： G02B21/16 ,  G02B21/0008 ,  G02B21/0088 ,  G02B21/12 ,  G02B21/26 ,  G02B21/362 ,  G02B21/365 ,  G02B27/149

摘要： The present invention provides a compact, inexpensive fluorescence microscope capable of high-resolution imaging with high light throughput suitable for use in both laboratory and field environments, and methods of use. A simple and inexpensive fluorescence microscope allows health care workers to perform various medical assays at the point of care instead of having to collect and transport biological samples to distant labs, and subsequently return the results to the patient. The microscope of the present invention is also useful for educational use and field use, and other uses as well.

摘要翻译： 本发明提供了一种紧凑，便宜的荧光显微镜，其能够具有适用于实验室和现场环境中的高光通量的高分辨率成像和使用方法。 简单而便宜的荧光显微镜允许医护人员在护理点执行各种医学检测，而不必将生物样品收集和运输到遥远的实验室，然后将结果返回给患者。 本发明的显微镜也可用于教育用途和现场使用以及其它用途。