公开(公告)号：US20210008172A1

公开(公告)日：2021-01-14

申请号：US16924810

申请日：2020-07-09

申请人： OPKO Biologics Ltd.

发明人： OREN HERSHKOVITZ ,  Lital Israeli-Yagev ,  Ahuva Bar-Ilan ,  Laura Moschcovich ,  Moran Golan

IPC分类号： A61K38/30 ,  C07K1/107 ,  C12N15/64 ,  C12N15/69 ,  A61K38/27 ,  A61K38/17

摘要： Compositions which include polypeptides comprising at least one carboxy-terminal peptide (CTP) of chorionic gonadotropin attached to the carboxy terminus or amino terminus of an insulin-like growth factor 1 (IGF-1) or IGF-1 variant. Polynucleotides encoding the same are disclosed. Pharmaceutical compositions and pharmaceutical formulations comprising the polypeptides and polynucleotides of the invention and methods of using and producing same are also disclosed.

公开(公告)号：US20190111125A1

公开(公告)日：2019-04-18

申请号：US16132862

申请日：2018-09-17

申请人： KATHOLIEKE UNIVERSITEIT LEUVEN

发明人： Kai DALLMEIER ,  Johan NEYTS

IPC分类号： A61K39/12 ,  C12N7/00 ,  C12N15/69

摘要： The invention relates to the use of a bacterial artificial chromosome (BAC) for the preparation of a vaccine, wherein the BAC comprises an inducible bacterial ori sequence for amplification of the BAC to more than 10 copies per bacterial cell. Plus a viral expression cassette comprising a cDNA of an attenuated RNA virus genome and comprising cis-regulatory elements for transcription of said viral cDNA in mammalian cells and for processing of the transcribed RNA into infectious viral RNA.

公开(公告)号：US09963698B2

公开(公告)日：2018-05-08

申请号：US14137737

申请日：2013-12-20

申请人： Commonwealth Scientific and Industrial Research Organisation

发明人： Michael Wayne Graham ,  Robert Norman Rice ,  Peter Michael Waterhouse

IPC分类号： C12N15/11 ,  C12N15/113 ,  A61K48/00 ,  C12N9/10 ,  C12N9/12 ,  C12N9/50 ,  C12N15/63 ,  C12N15/69 ,  C12N15/82 ,  C12N15/85

CPC分类号： C12N15/113 ,  A01K2217/05 ,  A61K48/00 ,  C12N9/1051 ,  C12N9/127 ,  C12N9/503 ,  C12N15/111 ,  C12N15/1131 ,  C12N15/63 ,  C12N15/69 ,  C12N15/8216 ,  C12N15/8218 ,  C12N15/8283 ,  C12N15/85 ,  C12N2310/111 ,  C12N2310/14 ,  C12N2310/531 ,  C12N2320/10 ,  C12N2320/30 ,  C12N2330/30 ,  C12N2330/50 ,  C12N2330/51 ,  C12N2800/108 ,  C12N2830/00 ,  C12N2830/002 ,  C12N2830/15 ,  C12N2830/38 ,  C12N2830/42 ,  C12N2830/55 ,  C12N2830/60 ,  C12N2840/20

摘要： The present invention relates generally to a method of modifying gene expression and to synthetic genes for modifying endogenous gene expression in a cell, tissue or organ of a transgenic organism, in particular a transgenic animal or plant. More particularly, the present invention utilizes recombinant DNA technology to post-transcriptionally modify or modulate the expression of a target gene in a cell, tissue organ or whole organism, thereby producing novel phenotypes. Novel synthetic genes and genetic constructs which are capable of repressing delaying or otherwise reducing the expression of an endogenous gene or target gene in an organism when introduced thereto are also provided.

公开(公告)号：US20170211077A1

公开(公告)日：2017-07-27

申请号：US15143493

申请日：2016-04-29

申请人： Intact Genomics

发明人： Cheng-Cang Charles Wu

IPC分类号： C12N15/80 ,  C12N15/52 ,  C12N15/10 ,  C12N15/69

CPC分类号： C12N15/80 ,  C12N15/1082 ,  C12N15/1093 ,  C12N15/52 ,  C12N15/69 ,  C12N2800/20 ,  C12N2800/70 ,  C12N2820/002 ,  C12N2820/10 ,  C12N2820/55 ,  C12Q2525/191

摘要： Fungal artificial chromosome (FAC) vectors are disclosed. A vector can be replicated in a bacterial or a fungal host, and can comprise an insert of heterologous DNA up to about 500 kilobases. A vector can be used for cloning and expressing a secondary metabolite (SM) gene cluster. An insert sequence can be modified by homologous recombination. A vector can be a plasmid comprising bacterial and fungal origins of replication, as well as bacterial and fungal selection marker genes. Also disclosed are vectors that can be integrated into a fungal genome, and dual function vectors which can be replicated in a bacterial or a fungal host and can also be integrated into a fungal genome. Also disclosed are methods of generating plasmid libraries including vectors comprising intact SM gene clusters.

公开(公告)号：US20160002647A1

公开(公告)日：2016-01-07

申请号：US14770074

申请日：2014-02-25

申请人： UNIVERSITY OF WASHINGTON THROUGH ITS CENTER FOR COMMERCIALIZATION

发明人： Maitreya DUNHAM ,  Ivan LIACHKO

IPC分类号： C12N15/81 ,  C12N15/69

CPC分类号： C12N15/81 ,  C12N15/69 ,  C12N15/815

摘要： A DNA sequence that functions as an origin in many different yeast species. From 1 to 17 mutations can be introduced into this sequence to improve its function across multiple yeasts. The resulting synthetic DNA sequence confers stable plasmid replication function in all yeast species tested, including but not limited to Saccharomyces cerevisiae, Lachancea kluyveri, Kluyveromyces lactis, Kluyveromyces wickerhammii, Hansenula polymorpha, and Pichia pastoris. Also provided are sequences that function as an optimal origin in the industrially useful Pichia pastoris.

摘要翻译： 在许多不同酵母菌种中作为起源的DNA序列。 可以将1至17个突变引入该序列以改善其在多种酵母中的功能。 所得合成DNA序列在所测试的所有酵母物质中都具有稳定的质粒复制功能，包括但不限于酿酒酵母，克鲁维酵母，乳酸克鲁维酵母，柳枝稷，多形汉逊酵母和巴斯德毕赤酵母。 还提供了在工业上有用的巴斯德毕赤酵母中起到最佳起源的序列。

公开(公告)号：US09045759B2

公开(公告)日：2015-06-02

申请号：US12656115

申请日：2010-01-19

申请人： James Arthur Williams

发明人： James Arthur Williams

IPC分类号： C12N15/00 ,  C12N15/69 ,  C12N15/70 ,  C12N1/20

CPC分类号： C12N15/69 ,  C12N15/70

摘要： The present invention relates to the production of covalently closed circular (ccc) recombinant DNA molecules such as plasmids, cosmids, bacterial artificial chromosomes (BACs), bacteriophages, viral vectors and hybrids thereof, and more particularly to vector modifications that improve production yield of said DNA molecules in fermentation culture.

摘要翻译： 本发明涉及共价闭合的环状（ccc）重组DNA分子如质粒，粘粒，细菌人造染色体（BAC），噬菌体，病毒载体及其杂合体的生产，更具体地涉及提高所述 发酵培养中的DNA分子。

公开(公告)号：US09029527B2

公开(公告)日：2015-05-12

申请号：US13866238

申请日：2013-04-19

申请人： Commonwealth Scientific and Industrial Research Organisation

发明人： Michael Wayne Graham ,  Robert Norman Rice

IPC分类号： C07H21/04 ,  C12N15/113 ,  C12N9/10 ,  C12N9/12 ,  C12N9/50 ,  C12N15/63 ,  C12N15/69 ,  C12N15/82 ,  C12N15/85

CPC分类号： C12N15/85 ,  C12N9/0071 ,  C12N9/1051 ,  C12N9/127 ,  C12N9/503 ,  C12N15/113 ,  C12N15/63 ,  C12N15/69 ,  C12N15/8216 ,  C12N15/8218 ,  C12N15/8283 ,  C12N2800/108 ,  C12N2830/00 ,  C12N2830/002 ,  C12N2830/15 ,  C12N2830/38 ,  C12N2830/42 ,  C12N2830/55 ,  C12N2830/60 ,  C12N2840/102 ,  C12N2840/20

摘要： The present invention relates generally to synthetic genes for modifying endogenous gene expression in a cell, tissue or organ of a transgenic organism, in particular a transgenic animal or plant. More particularly, the present invention provides novel synthetic genes and genetic constructs which are capable of repressing delaying or otherwise reducing the expression of an endogenous gene or a target gene in an organism when introduced thereto.

摘要翻译： 本发明一般涉及用于修饰转基因生物体，特别是转基因动物或植物的细胞，组织或器官中的内源基因表达的合成基因。 更具体地说，本发明提供了新的合成基因和遗传构建体，其能够在引入生物体时抑制延迟或以其他方式降低生物体中的内源基因或靶基因的表达。

公开(公告)号：US06955902B2

公开(公告)日：2005-10-18

申请号：US09849866

申请日：2001-05-04

申请人： Ilya Chumakov ,  Hiroaki Tanaka

发明人： Ilya Chumakov ,  Hiroaki Tanaka

IPC分类号： C12N15/09 ,  C12N9/38 ,  C12N15/10 ,  C12N15/63 ,  C12N15/69 ,  C12Q1/68 ,  C12N15/11 ,  C12N15/64 ,  C12Q1/00 ,  C12Q1/04

CPC分类号： C12Q1/6841 ,  C12N9/16 ,  C12N9/2471 ,  C12N15/102 ,  C12N15/63 ,  C12N15/69 ,  C12Q1/6869 ,  C12Y302/01023

摘要： High throughput DNA sequencing vectors for generating nested deletions using enzymatic techniques and/or transposition-based techniques are disclosed. Methods of constructing contigs of long DNA sequences and methods of generating nested deletions are also disclosed. A truncated lacZ derivative useful in measuring the copy number of the lacZ derivative in a host cell is also disclosed.

摘要翻译： 公开了用于使用酶技术和/或基于置换的技术产生嵌套缺失的高通量DNA测序载体。 还公开了构建长DNA序列的重叠群的方法和产生嵌套缺失的方法。 还公开了可用于测量宿主细胞中lacZ衍生物的拷贝数的截短的lacZ衍生物。

公开(公告)号：US06864087B2

公开(公告)日：2005-03-08

申请号：US10209792

申请日：2002-07-31

申请人： Waclaw Szybalski ,  Jadwiga Wild ,  Zdenka Hradecna

发明人： Waclaw Szybalski ,  Jadwiga Wild ,  Zdenka Hradecna

IPC分类号： C12N15/63 ,  C12N15/69 ,  C12N15/70

CPC分类号： C12N15/63

摘要： A single-copy BAC vector (containing or lacking an insert) is converted in a host cell into a conditional high-copy BAC vector by introducing a conditional origin of replication into the single-copy BAC vector. The conditional ori is introduced by site-specific recombination between the SC BAC vector and a vector that contains the conditional ori. The host cell comprises a recombinase that recognizes a site-specific recombination site on both the BAC vector and the conditional ori vector. In the presence of the recombinase, the conditional ori-containing vector recombines into the BAC vector to produce a high-copy BAC vector that can be conditionally amplified by activating the conditional origin of replication on command.

摘要翻译： 通过将有条件的复制起点引入到单拷贝BAC载体中，将单个副BAC载体（包含或缺少插入片段）在宿主细胞中转化为条件高拷贝BAC载体。 有条件的ori是通过SC BAC载体与含有条件的ori的载体之间的位点特异性重组引入的。 宿主细胞包含识别BAC载体和条件性ori载体上的位点特异性重组位点的重组酶。 在存在重组酶的情况下，将含条件的载体载体重组到BAC载体中以产生可通过在命令上激活条件复制起点而有条件地扩增的高拷贝BAC载体。

公开(公告)号：US5776746A

公开(公告)日：1998-07-07

申请号：US644664

申请日：1996-05-01

申请人： Dan W. Denney, Jr.

发明人： Dan W. Denney, Jr.

IPC分类号： A61K39/00 ,  A61P35/00 ,  C07K14/52 ,  C07K14/54 ,  C07K14/705 ,  C07K14/725 ,  C07K14/74 ,  C07K16/00 ,  C12N9/00 ,  C12N9/06 ,  C12N15/13 ,  C12N15/85 ,  C12P21/08 ,  C12N5/10 ,  C12N15/69 ,  C12N15/87

CPC分类号： C12N9/003 ,  C07K14/52 ,  C07K14/5428 ,  C07K14/7051 ,  C07K14/70539 ,  C07K14/70596 ,  C07K16/00 ,  C12N15/85 ,  C12N9/00 ,  A61K39/00 ,  C07K2319/00 ,  C12N2800/108 ,  C12N2830/00 ,  C12N2830/15 ,  C12N2830/60 ,  C12N2830/85 ,  C12N2840/20 ,  C12N2840/44

摘要： The present invention provides improved methods for the amplification and expression of recombinant genes in cells. The methods of the present invention permit the isolation of cell lines which have co-amplified input recombinant sequences which encode an amplifiable marker, one or more expression vectors encoding a protein of interest and optionally a selectable marker. The present methods allow the efficient isolation of amplified cell lines which express the protein(s) of interest in a relatively short period of time. The present invention also provides compositions comprising amplified T lymphoid cell lines.

摘要翻译： 本发明提供了在细胞中扩增和表达重组基因的改进方法。 本发明的方法允许分离具有编码可扩增标记的共扩增输入重组序列的细胞系，一种或多种编码目的蛋白质和任选的选择性标记的表达载体。 本发明的方法允许在相对短的时间段内有效分离表达所关注蛋白质的扩增细胞系。 本发明还提供包含扩增的T淋巴细胞系的组合物。