公开(公告)号：US10584362B2

公开(公告)日：2020-03-10

申请号：US15303944

申请日：2014-07-30

申请人： Xiamen University

发明人： Ning He ,  Haosheng Yao ,  Yu Liu ,  Wencheng Yu ,  Shan Yan ,  Yuanpeng Wang ,  Qingbiao Li

IPC分类号： C12P19/04 ,  C12N1/20 ,  C12P21/02 ,  C07K14/32

摘要： The present application provides a method for simultaneously synthesizing a biological flocculant with polysaccharide and γ-PGA as the active components by using Bacillus licheniformis. Bacillus licheniformis is inoculated to a slant culture medium to be cultured; a single colony on a fresh plate is inoculated to a seed culture medium to be cultured; and a seed fermentation broth is inoculated to a fermentation culture medium to be cultured, and then the biological flocculant having two different components is obtained. The flocculant synthesized in the present invention is high in activity and good in thermal stability; and especially, the flocculation effect of polysaccharide is relatively superior under acidic and neutral conditions, and the flocculation activity of γ-PGA is relatively higher under neutral and alkaline environments, which can satisfy a relatively large pH application range.

公开(公告)号：US20200002738A1

公开(公告)日：2020-01-02

申请号：US16096351

申请日：2017-04-28

申请人： KOREA UNIVERSITY RESEARCH AND BUSINESS FOUNDATION

发明人： Kyoung Heon KIM ,  Damao WANG ,  Do-Hyoung KIM

IPC分类号： C12P19/02 ,  C12P19/04 ,  C12N9/24 ,  C12N15/52

摘要： The present invention relates to a novel β-1,3-1,6-endoglucanase producing, from β-glucan, oligosaccharides or glucose. More specifically, the present invention provides an effect of producing oligosaccharides or glucose of various degrees of polymerization in high yields by using a β-1,3-1,6-endoglucanase exhibiting β-1,3-endoglucanase and ββ-1,6-endoglucanase activity on β-glucan and exhibiting transglycosylation activity on laminarioligosaccharide.

公开(公告)号：US10513680B2

公开(公告)日：2019-12-24

申请号：US15308341

申请日：2015-05-07

申请人： COMMISSARIAT A L'ENERGIE ATOMIQUE ET AUX ENERGIES ALTERNATIVES

发明人： Miriam Schulz-Raffelt ,  Vincent Chochois ,  Yonghua Li-Beisson ,  Gilles Peltier

IPC分类号： C12N1/12 ,  C12P19/04 ,  C12N9/12 ,  C12P7/64

摘要： The present invention relates to a method for producing biomass feedstock, by cultivating green microalgae cells in which the expression and/or the activity of the dual-specificity tyrosine-phosphorylation-regulated kinase (DYRKP-1) protein is altered, inducing reserve accumulation and/or increase in biomass production by said microalgae.

公开(公告)号：US20190352685A1

公开(公告)日：2019-11-21

申请号：US16402779

申请日：2019-05-03

申请人： DSM IP ASSETS B.V.

发明人： ELLEN SPEETJENS ,  PETER PHILIP LANKHORST

IPC分类号： C12P19/04 ,  A61K36/064 ,  A23K50/30 ,  A23K50/75 ,  A61K31/716 ,  C12P19/14 ,  C08B37/00 ,  A23K20/163

摘要： The present invention provides a process for the treatment of a composition comprising yeast cell walls comprising β-1,3-glucans which are insoluble when extracted with water and partially soluble when extracted with DMSO, the process comprising contacting said composition with laminaripentaose-producing-β-1,3-glucanase and inactivating the laminaripentaose-producing-β-1,3-glucanase to result in a composition comprising yeast cell walls wherein the β-1,3-glucans have an improved solubility in DMSO and the ratio of β-glucans soluble in DMSO compared to water is greater than or equal to 2.

公开(公告)号：US10471135B2

公开(公告)日：2019-11-12

申请号：US15008891

申请日：2016-01-28

申请人： Glycanova AS

发明人： Bjoern Kristiansen

IPC分类号： A61K39/00 ,  A61K31/716 ,  A61K36/07 ,  C12P19/04 ,  A61K31/7004

摘要： A process is described for the production of an immunostimulant by submerged cultivation of Ganoderma lucidum in which mycelium from agar plates or a fermentation broth is added to a liquid medium in a shake flask or a bioreactor containing nutrients such as malt extract, yeast extract, peptone and glucose having access to air or to which air is added, and which is kept in constant movement at approx. 28° C. At the proper conditions, there will be an increase in the production of extracellular lentinan, which is shown to be a better immunostimulant than intracellular lentinan. The extracellular product is precipitated from the growth medium by means of methods for the precipitation of microbial polysaccharide.

公开(公告)号：US10471134B2

公开(公告)日：2019-11-12

申请号：US16058898

申请日：2018-08-08

申请人： The Regents of the University of California

发明人： Daniel Garrido ,  J. Bruce German ,  Carlito B. Lebrilla ,  David A. Mills

IPC分类号： A61K38/16 ,  A61K38/47 ,  C12N9/24 ,  A23L2/52 ,  A61K31/715 ,  A61K35/741 ,  A23C9/12 ,  C12P21/00 ,  C12P19/04 ,  A23L33/195 ,  A61K38/40 ,  C07K14/79 ,  A61K38/00

摘要： Provided herein are deglycosylating enzymes that remove a broad range of N-glycans from N-glycosylated proteins. Further provided are methods of recombinantly producing and expressing the deglycosylating enzymes. The presently described deglycosylating enzymes can be used to produce free glycans for characterization, and for prebiotic and immunostimulatory uses. In addition, the presently described deglycosylating enzymes can be used to produce deglycosylated proteins for characterization, to improve digestion, and to reduce immunogenicity.

公开(公告)号：US20190338326A1

公开(公告)日：2019-11-07

申请号：US16398483

申请日：2019-04-30

申请人： Bio Capital Holdings, LLC

发明人： Raul CUERO RENGIFO ,  Juliana LONDOÑO MURILLO ,  Mariana SANCHEZ LONDOÑO ,  Daniel AGUDELO VALENCIA ,  Juan SANCHEZ BUSTAMANTE ,  Mario Antonio FRANCO JIMENEZ ,  Lina Clemencia SANCHEZ BUSTAMANTE

IPC分类号： C12P19/04 ,  C12N9/10 ,  C12N15/81 ,  C12N15/72 ,  C12N1/16 ,  C12N1/20

摘要： Described herein are biological devices and methods for using the same to produce carbo sugars. The biological devices include microbial cells transformed with a DNA construct containing genes for producing a cellulose synthase and galactomannan galactosyltransferase. In some instances, the biological devices also include a gene for lipase. Methods for altering the viscosity of petroleum oil using the carbo sugars are also described herein. Finally, methods for degreasing or decontaminating water mixed with petroleum oil or other fatty substances or a surface coated with petroleum oil or other fatty substances using the carbo sugars are described herein.

公开(公告)号：US10465175B2

公开(公告)日：2019-11-05

申请号：US15794926

申请日：2017-10-26

申请人： Samsung Electronics Co., Ltd

发明人： Hongsoon Rhee ,  Jinkyu Kang ,  Jinhwan Park ,  Dongsik Yang

IPC分类号： C12N9/12 ,  C12P19/04 ,  C12N9/04 ,  C12N1/20

摘要： Provided are a recombinant microorganism including a genetic modification that increases a pyruvate, phosphate dikinase activity, a method of producing cellulose using the same, and a method of producing a microorganism having enhanced cellulose productivity.

公开(公告)号：US10428362B2

公开(公告)日：2019-10-01

申请号：US15976246

申请日：2018-05-10

申请人： E I DU PONT DE NEMOURS AND COMPANY

发明人： Kevin D. Nagy ,  Erwin F. Columbus-Hago ,  Jayarama K. Shetty ,  Susan Marie Hennessey ,  Robert Dicosimo ,  Ling Hua ,  Rodrigo Ramirez ,  Zhongmei Tang ,  Zheyong Yu

IPC分类号： C12P19/14 ,  C12P7/14 ,  C12P19/02 ,  C12P19/18 ,  C12P19/04 ,  C12P19/16 ,  C12P7/06

摘要： A method is disclosed for hydrolyzing an alpha-1,5 glucosyl-fructose linkage in a saccharide (disaccharide or oligosaccharide) such as leucrose. This method comprises contacting the saccharide with an alpha-glucosidase enzyme such as transglucosidase or glucoamylase under suitable conditions, during which contacting step the enzyme hydrolyzes at least one alpha-1,5 glucosyl-fructose linkage of the saccharide. This method is useful for reducing the amount of leucrose in a filtrate isolated from a glucan synthesis reaction, for example.

公开(公告)号：US10428101B2

公开(公告)日：2019-10-01

申请号：US14897596

申请日：2014-06-16

申请人： The Regents of the University of California

发明人： Xi Chen ,  Hai Yu ,  Joel Hwang

IPC分类号： C07H15/04 ,  C07H1/00 ,  C07H15/08 ,  C12P19/04 ,  C12P19/18 ,  C12P19/44

摘要： The present invention provides a method of preparing an isolated oligosaccharide or glycolipid by preparing the oligosaccharide or glycolipid having a hydrophobic group, such as a perfluorinated alkyl or a fatty acid, and separating the oligosaccharide or glycolipid using a solid-phase extraction cartridge.