公开(公告)号：US10128093B2

公开(公告)日：2018-11-13

申请号：US15576874

申请日：2016-05-26

Applicant: DH Technologies Development Pte. Ltd.

Inventor： Stephen A. Tate ,  John Lawrence Campbell

IPC: H01J49/00

Abstract: Systems and methods prevent potentially convolved precursor ion peaks from being excluded in subsequent cycles of an IDA experiment so that additional product ion data is collected. A sample is ionized producing an ion beam. A plurality of cycles of an IDA experiment are performed on the ion beam. During each cycle of the IDA experiment and for each precursor ion peak on a filtered peak list produced in the filtering step of each cycle, several steps are performed. The precursor ion peak is identified in the precursor ion spectrum produced in the MS survey scan step of the cycle. It is determined if the precursor ion peak in the precursor ion spectrum includes a feature of convolution. If the precursor ion peak includes a feature of convolution, the precursor ion peak is prevented from being excluded in a filtering step of one or more subsequent cycles.

公开(公告)号：US10078091B2

公开(公告)日：2018-09-18

申请号：US14401427

申请日：2013-05-17

Applicant: DH Technologies Development Pte. Ltd. ,  Oregon Health and Science University

Inventor： Andrea DeBarber ,  Subhasish Purkayastha ,  Robert D. Steiner ,  Michal Weinstock

IPC: G01N33/00 ,  G01N33/92 ,  G01N33/58 ,  H01J49/00 ,  G01N33/68

CPC classification number: G01N33/92 ,  G01N33/58 ,  G01N33/6848 ,  G01N33/6893 ,  G01N2333/90251 ,  G01N2400/00 ,  G01N2800/04 ,  G01N2800/065 ,  G01N2800/385 ,  H01J49/0031

Abstract: A method, a labeling reagent, sets of labeling reagents, and labeling techniques are provided for the analysis of ketosterol biomarkers such as bile acid precursors from human plasma, serum or whole blood. This method is used for new born screening for Cerebrotendinous Xanthomatosis (CTX). Methods for labeling, analyzing, and quantifying ketosterol biomarkers are also disclosed as are methods that also use mass spectrometry.

公开(公告)号：US20180259481A1

公开(公告)日：2018-09-13

申请号：US15761478

申请日：2016-09-20

Applicant: DH Technologies Development Pte. Ltd.

Inventor： Mark Lies ,  Erika Lin ,  Randy Roushall ,  Marcia Santos

IPC: G01N27/447 ,  B01L3/00

CPC classification number: G01N27/44743 ,  B01L3/502 ,  B01L9/065 ,  B01L2300/0838 ,  B01L2400/0421 ,  G01N27/44791

Abstract: Vials for reducing the volume of sample that must be contained within the vial when interfacing with a variety of analytical instruments are provided herein, and particularly, for use in analytical instruments that interact with a sample via a “polar” or asymmetric probe, including in capillary electrophoresis, liquid chromatography, and gas chromatography, by way of non-limiting example. In accordance with various aspects of the present teachings, the vials can reduce the waste of expensive and/or scarce samples common in the round vials and vial inserts conventionally used in the art.

公开(公告)号：US10062556B2

公开(公告)日：2018-08-28

申请号：US15540750

申请日：2015-12-21

Applicant: DH Technologies Development PTE Ltd.

Inventor： Takashi Baba

IPC: H01J49/42 ,  H01J49/26 ,  H01J49/00 ,  H01J49/06

CPC classification number: H01J49/0072 ,  H01J49/0054 ,  H01J49/062 ,  H01J49/063

Abstract: A method and apparatus for conducting reactions between precursor ions and reagent ions, for example, a reaction between a precursor cation and an electron, such as ECD, are disclosed. The apparatus comprises first, second, and third pathways, each of which extends at least partially along a central axis, and wherein the second central axis is orthogonal to the first and third central axes. Charged species can be introduced into the second pathway as the ions are transmitted therethrough, thereby increasing precursor ion and charged species interaction without simultaneous trapping of the species.

公开(公告)号：US20180240658A1

公开(公告)日：2018-08-23

申请号：US15750550

申请日：2016-08-09

Applicant: DH Technologies Development Pte. Ltd.

Inventor： David Michael Cox ,  Gordana Ivosev

IPC: H01J49/00 ,  H01J49/04 ,  H01J49/26

CPC classification number: H01J49/0031 ,  H01J49/0027 ,  H01J49/004 ,  H01J49/0045 ,  H01J49/04 ,  H01J49/26

Abstract: An m/z range of an ion beam is divided into two or more precursor ion mass selection windows. A pattern of two or more different window m/z ranges to be used during two or more successive cycles for at least one precursor ion mass selection window is determined. The pattern includes an initial window m/z range and one or more successively different window m/z ranges. Each of the one or more successively different window m/z ranges includes at least a portion of the initial window m/z range. A tandem mass spectrometer is instructed to select and fragment the two or more precursor ion mass selection windows during each cycle of a plurality of cycles and to repeatedly use the pattern for each group of two or more successive cycles of the plurality of cycles for the selection and fragmentation of the at least one precursor ion mass selection window.

公开(公告)号：US20180218895A1

公开(公告)日：2018-08-02

申请号：US15107141

申请日：2014-11-28

Applicant: DH Technologies Development PTE Ltd.

Inventor： Bruce Andrew Collings ,  John Vandermey

IPC: H01J49/42 ,  H01J49/00

CPC classification number: H01J49/427 ,  H01J49/0027 ,  H01J49/004 ,  H01J49/4225 ,  H01J49/423

Abstract: A method and apparatus for clearing ions from a multipole ion transmission device which includes introducing a DC or RF clear out pulse to one or more of the rods of the multipole device. The DC pulse is selected so as to supply sufficient kinetic energy to the ions to overcome a pseudo-potential trapping well generated by the RF potentials of the ion transmission device. For an RF pulse, the auxiliary RF signal uses frequencies that correspond to the ejected ion's frequencies of motion. In select embodiments, the multipole device can be a quadrupole or the apparatus can be part of a tandem mass spectrometer.

公开(公告)号：US10001456B2

公开(公告)日：2018-06-19

申请号：US15511712

申请日：2015-08-28

Applicant: DH Technologies Development PTE Ltd.

Inventor： John L Campbell ,  Yves LeBlanc ,  Chang Liu

IPC: C07K1/14 ,  C07K1/00 ,  B01D59/44 ,  B01D15/38 ,  B01D15/36 ,  C12Q1/68 ,  G01N33/53 ,  H01J49/26 ,  G01N27/62 ,  H01J49/04 ,  H01J49/10

CPC classification number: G01N27/624 ,  G01N33/6803 ,  H01J49/04 ,  H01J49/10

Abstract: Methods and systems for separating and/or quantifying peptides using differential mobility spectrometry (DMS) are provided herein. In accordance with various aspects of the applicant's teachings, the methods and systems can provide for the separation of one or more peptides, for example, peptides that may be difficult to separate with conventional techniques, such as mass spectrometry (MS), by complexing the peptides with a metal cation (e.g., Ca2+) prior to DMS. In some aspects, the present teachings can prevent proton stripping from ionized peptides that can occur in DMS to prevent unintended and/or undesirable alterations to the peptide's charge state distribution.

公开(公告)号：US20180166264A1

公开(公告)日：2018-06-14

申请号：US15576875

申请日：2016-05-26

Applicant: DH Technologies Development Pte. Ltd.

Inventor： Lyle Lorrence Burton ,  Gordana Ivosev

IPC: H01J49/00 ,  G06F19/00

CPC classification number: H01J49/0036 ,  G06F19/709 ,  H01J49/0072

Abstract: One or more known compounds of a sample are ionized. At least one precursor ion corresponding to a compound of the one or more known compounds is selected and fragmented, producing a product ion mass spectrum for the precursor ion. An m/z tolerance probability function that varies from 1 to 0 with increasing values of an m/z difference between two mass peaks and that includes one or more values between 1 and 0 is received. A library product ion mass spectrum for the at least one compound is retrieved from a memory. An m/z difference between at least one experimental product ion mass peak in the product ion mass spectrum and at least one library product ion mass peak in the library product ion mass spectrum is calculated. An m/z tolerance probability that determines if the two peaks are corresponding peaks is calculated from the m/z difference using the probability function.

公开(公告)号：US09991104B2

公开(公告)日：2018-06-05

申请号：US14401030

申请日：2013-04-19

Applicant: DH TECHNOLOGIES DEVELOPMENT PTE. LTD.

Inventor： Bruce Andrew Collings ,  Martian Dima ,  Gordana Ivosev

IPC: H01J49/00 ,  H01J49/02 ,  G01T1/17

CPC classification number: H01J49/0027 ,  G01T1/171 ,  H01J49/0009 ,  H01J49/0036 ,  H01J49/025

Abstract: Systems and methods are provided to perform dead time correction. An observed ion count rate is obtained using a non-paralyzable detection system of a mass spectrometer. The detection system includes an ion detector, a comparator/discriminator, a mono-stable circuit and a counter. The non-paralyzable detection system exhibits dead time extension at high count rates. The extension of the dead time occurs because the mono-stable circuit requires a rising edge to trigger and can only be triggered again after the output pulse from the comparator/discriminator has gone low. This allows a second comparator/discriminator pulse arriving just before the end of the dead time started by a first comparator/discriminator pulse to extend the dead time to the trailing edge of the second comparator/discriminator pulse. A true ion count rate is calculated by performing dead time correction of the observed ion count rate.

公开(公告)号：US20180108521A1

公开(公告)日：2018-04-19

申请号：US15556698

申请日：2016-03-08

Applicant: DH Technologies Development Pte Ltd.

Inventor： John L. Campbell

IPC: H01J49/00 ,  G01N30/72 ,  G01N30/86 ,  H01J49/42

CPC classification number: H01J49/0081 ,  G01N30/7233 ,  G01N30/8631 ,  G01N30/8644 ,  G01N30/8689 ,  H01J49/0027 ,  H01J49/0036 ,  H01J49/004 ,  H01J49/4265

Abstract: A method and apparatus for improving the quality of spectra of a sample obtained from a tandem mass spectrometer system containing an ion trap. The method and apparatus includes the setting of an upper and lower threshold limit on peak intensity and only triggering an enhanced product ion scan when a detected intensity of a peak in an initial scan falls between the upper and lower threshold limits. The spectra obtained from an enhanced product ion scan conducted in this manner are useful in library matching of spectra. The ion trap may be a linear ion trap and the sample may be a peptide.