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公开(公告)号：US08293514B2
公开(公告)日：2012-10-23
申请号：US12781230
申请日：2010-05-17
IPC分类号： C12N9/12
CPC分类号： C12P13/08 , A23L33/135 , A23L33/175 , C12N9/1235 , C12P13/04
摘要： An isolated mutant of a coryneform bacterium comprising a gene coding for a polypeptide having GTP-pyrophosphate kinase activity, wherein said polypeptide comprises an amino acid sequence in which one of the proteinogenic amino acids other than L-proline is present in position 38 or a corresponding or comparable position. In addition, an isolated polynucleotide encoding a polypeptide having GTP-pyrophosphate kinase enzyme activity, a vector comprising the isolated polynucleotide, a recombinant microorganism comprising the vector, and a process for preparing the recombinant coryneform bacterium is described. A method for over-expressing a GTP-pyrophosphate kinase, a method of preparing an L-amino acid, an L-lysine comprising and L-tryptophan comprising feed is also described.
摘要翻译： 棒状细菌的分离突变体，其包含编码具有GTP-焦磷酸激酶活性的多肽的基因，其中所述多肽包含氨基酸序列，其中除了L-脯氨酸以外的蛋白原氨基酸之一存在于位置38或相应的 或相当的地位。 此外，描述了编码具有GTP焦磷酸激酶酶活性的多肽的分离的多核苷酸，包含分离的多核苷酸的载体，包含载体的重组微生物和制备重组棒状杆菌型细菌的方法。 还描述了过表达GTP焦磷酸激酶的方法，制备L-氨基酸的方法，包含L-赖氨酸的L-赖氨酸和包含L-色氨酸的饲料。
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公开(公告)号：US08143032B1
公开(公告)日：2012-03-27
申请号：US13082542
申请日：2011-04-08
申请人： Mechthild Rieping , Georg Thierbach
发明人： Mechthild Rieping , Georg Thierbach
CPC分类号： C12N9/0006 , C12P13/08 , C12Y101/01003
摘要： Alleles of the thrA gene from Enterobacteriaceae encoding desensitized aspartokinase I-homoserine dehydrogenase I enzymes and methods for the fermentative production of L-threonine using bacteria containing these alleles.
摘要翻译： 来自编码脱敏天冬氨酸激酶I-高丝氨酸脱氢酶I的肠杆菌科的thrA基因的等位基因以及使用含有这些等位基因的细菌发酵生产L-苏氨酸的方法。
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公开(公告)号：US07585650B2
公开(公告)日：2009-09-08
申请号：US11790063
申请日：2007-04-23
CPC分类号： C12P13/08 , C07K14/34 , C12P13/227
摘要： The invention relates to mutants and alleles of the zwf gene of coryneform bacteria, which encode variants of the Zwf subunit of glucose 6-phosphate dehydrogenase (EC: 1.1.1.49), and to processes for preparing amino acids, in particular L-lysine and L-tryptophan, by using bacteria which harbor said alleles.
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公开(公告)号：US20080131927A1
公开(公告)日：2008-06-05
申请号：US11383253
申请日：2006-05-15
CPC分类号： C12P13/08 , C12N9/0006 , C12P13/227
摘要： The invention relates to mutants and alleles of the opcA gene of coryneform bacteria, which encode variants of the OpcA subunit of glucose 6-phosphate dehydrogenase (EC: 1.1.1.49), and to processes for preparing amino acids, in particular L-lysine and L-tryptophan, by using bacteria which harbor said alleles.
摘要翻译： 本发明涉及编码葡萄糖-6-磷酸脱氢酶（EC：1.1.1.49）的OpcA亚基变体的棒状杆菌细菌的opcA基因的突变体和等位基因，以及制备氨基酸，特别是L-赖氨酸和 L-色氨酸通过使用含有所述等位基因的细菌。
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75.发明授权公开(公告)号：US07262034B2
公开(公告)日：2007-08-28
申请号：US10480486
申请日：2002-05-31
申请人： Daniela Kruse , Georg Thierbach
发明人： Daniela Kruse , Georg Thierbach
CPC分类号： C12P13/02
摘要： The invention provides a process for the fermentative preparation of D-pantothenic acid and/or salts thereof or feedstuffs additives comprising these by fermentation of microorganisms of the Bacillus group, in particular those which already produce D-pantothenic acid, which comprises enhancing, in particular over-expressing, in the microorganisms one or more of the nucleotide sequence(s) which code(s) for the gene or ORF serA, serC, ywpJ or glyA.
摘要翻译： 本发明提供了通过发酵芽孢杆菌属组的微生物，特别是已经生产D-泛酸的那些的D-泛酸和/或其盐的发酵制剂或包含这些添加剂的饲料添加剂的方法，其包括增强，特别是 在微生物中过表达一个或多个编码基因或ORF serA，serC，ywpJ或glyA的核苷酸序列。
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公开(公告)号：US20070141680A1
公开(公告)日：2007-06-21
申请号：US11705714
申请日：2007-02-14
CPC分类号： C12P13/04 , C12N9/0006
摘要： The invention relates to mutants and alleles of the coryneform bacterium mqo gene which encodes malate quinone oxidoreductases which contain any amino acid apart from L-serine at position 111, or a comparable position, in the amino acid sequence, and to processes for fermentatively preparing amino acids, preferably L-lysine, L-tryptophan and L-proline, using bacteria which comprise these alleles.
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公开(公告)号：US20070092950A1
公开(公告)日：2007-04-26
申请号：US10567749
申请日：2004-07-29
摘要： The invention provides an improved process for the fermentative preparation of L-threonine using L-threonine-producing bacteria from the family Enterobacteriaceae.
摘要翻译： 本发明提供使用来自肠杆菌科的L-苏氨酸生产细菌的L-苏氨酸的发酵制剂的改进方法。
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公开(公告)号：US20060252133A1
公开(公告)日：2006-11-09
申请号：US10566606
申请日：2004-07-27
摘要： The invention relates to an improved process for the fermentative preparation of L-threonine using bacteria of the Enterobacteriaceae family which produce L-threonine.
摘要翻译： 本发明涉及利用产生L-苏氨酸的肠杆菌科的细菌发酵L-精氨酸的方法。
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79.发明授权Nucleotide sequences coding for the thrE gene and process for the enzymatic production of L-threonine using coryneform bacteria 失效编码thrE基因的核苷酸序列和使用棒状细菌酶促生产L-苏氨酸的方法公开(公告)号：US06913909B2
公开(公告)日：2005-07-05
申请号：US09963521
申请日：2001-09-27
申请人： Petra Ziegler , Lothar Eggeling , Hermann Sahm , Georg Thierbach
发明人： Petra Ziegler , Lothar Eggeling , Hermann Sahm , Georg Thierbach
IPC分类号： C12N15/09 , C07K14/34 , C12N1/21 , C12N15/01 , C12N15/31 , C12N15/77 , C12P13/08 , C12R1/15 , C12N1/20 , C12N15/00 , C12P21/06 , C12Q1/48
摘要： The invention relates to preferably recombinant DNA derived from Corynebacterium and replicable in coryneform microorganisms, which contains at least one nucleotide sequence that codes for the thrE gene, and a process for the production of L-threonine, which is characterised in that the following steps are carried out: a) Fermentation of microorganisms in which at least the thrE gene is amplified (overexpressed), optionally in combination with further genes, b) Enrichment of the L-threonine in the medium or in the cells of the microorganisms, and c) Isolation of the L-threonine.
摘要翻译： 本发明涉及优选来源于棒状杆菌的重组DNA，并可在包含编码thrE基因的至少一个核苷酸序列的棒状细菌中复制，以及L-苏氨酸的制备方法，其特征在于以下步骤为 进行：a）至少将thrE基因扩增（过表达）的微生物发酵，任选与其它基因组合，b）在培养基中或微生物细胞中富集L-苏氨酸，和c） L-苏氨酸的分离。
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公开(公告)号：US06667166B2
公开(公告)日：2003-12-23
申请号：US09887054
申请日：2001-06-25
申请人： Georg Thierbach , Nicole Dusch
发明人： Georg Thierbach , Nicole Dusch
IPC分类号： C12P1304
CPC分类号： C12N9/1205 , C12P7/42 , C12R1/15
摘要： The present invention provides processes for preparing D-pantothenic acid using Coryneform bacteria having an enhanced pfkA gene.
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