公开(公告)号：US20230303996A1

公开(公告)日：2023-09-28

申请号：US18023316

申请日：2020-08-28

Applicant: Hewlett-Packard Development Company, L.P.

Inventor： Jacob Shane Sasser ,  Alexander Govyadinov ,  Brian J. Keefe ,  David S. Clague ,  Natalie Taylor Weisenburger ,  Nicolas Robert Medjo

IPC: C12N15/10 ,  B01L3/00

CPC classification number: C12N15/1006 ,  B01L3/502761 ,  B01L2400/086 ,  B01L2200/0652

Abstract: In one example in accordance with the present disclosure, a fluid manipulation system is described. The fluid manipulation system includes a microfluidic channel through which fluid is to flow. The fluid includes biomolecules to be separated. The fluid manipulation system also includes at least one array of biomolecule-capturing pillars disposed within the microfluidic channel to capture biomolecules from the fluid. Barriers rise from a surface of the microfluidic channel. The barriers span a width of the microfluidic channel orthogonal to a flow of the fluid to induce vortices in the fluid flow.

公开(公告)号：US20230287479A1

公开(公告)日：2023-09-14

申请号：US18016860

申请日：2020-07-21

Applicant: Hewlett-Packard Development Company, L.P.

Inventor： Viktor Shkolnikov ,  Alexander Govyadinov

IPC: C12Q1/6823 ,  B01L3/00 ,  B01L7/00

CPC classification number: C12Q1/6823 ,  B01L3/502761 ,  B01L7/00 ,  B01L2200/0652 ,  B01L2200/16 ,  B01L2300/0654 ,  B01L2400/0487 ,  B01L2300/088 ,  B01L2300/1805

Abstract: An example method, consistent with the present disclosure, includes receiving in a microfluidic channel, a fluid sample and a reagent, where the reagent includes a reporter nucleic acid labeled with a detectable ligand. The method further includes identifying a target nucleic acid in the fluid sample using a guide ribonucleic acid (gRNA) and a programmable nuclease immobilized in a side channel fluidically coupled to the microfluidic channel. Responsive to identifying the target nucleic acid in the fluid sample, the method includes causing cleavage of the detectable ligand, and detecting the detectable ligand from the cleaved reporter nucleic acid in the side channel.

公开(公告)号：US11686327B2

公开(公告)日：2023-06-27

申请号：US16493344

申请日：2017-04-07

Applicant: Hewlett-Packard Development Company, L.P.

Inventor： Erik D. Torniainen ,  Alexander Govyadinov ,  Pavel Kornilovich ,  David P. Markel

IPC: F04F7/00 ,  B01L3/00 ,  B81B1/00 ,  F04B53/10

CPC classification number: F04F7/00 ,  B01L3/50273 ,  B01L3/502738 ,  B81B1/006 ,  F04B53/10 ,  B01L2200/141 ,  B01L2200/16 ,  B01L2400/0605 ,  B81B2201/051 ,  B81B2203/0338

Abstract: The present disclosure is drawn to inertial pumps. An inertial pump can include a microfluidic channel, a fluid actuator located in the microfluidic channel, and a check valve located in the microfluidic channel. The check valve can include a moveable valve element, a narrowed channel segment located upstream of the moveable valve element, and a blocking element formed in the microfluidic channel downstream of the moveable valve element. The narrowed channel segment can have a width less than a width of the moveable valve element so that the moveable valve element can block fluid flow through the check valve when the moveable valve element is positioned in the narrowed channel segment. The blocking element can be configured such that the blocking element constrains the moveable valve element within the check valve while also allowing fluid flow when the moveable valve element is positioned against the blocking element.

公开(公告)号：US20230131266A1

公开(公告)日：2023-04-27

申请号：US17507152

申请日：2021-10-21

Applicant: HEWLETT-PACKARD DEVELOPMENT COMPANY, L.P.

Inventor： Viktor Shkolnikov ,  Alexander Govyadinov

IPC: C12N15/02 ,  C12M1/42 ,  C12M1/36 ,  C12M3/06

Abstract: A method of alignment and fusion of cells with an electrical field includes firing a first fluid dispenser of an electrofusion device until a first impedance sensor in a first microfluidic channel of the electrofusion device detects a presence of a first cell. The method includes firing a second fluid dispenser of the electrofusion device until a second impedance sensor in a second microfluidic channel of the electrofusion device detects a presence of a second cell. The method includes moving the first cell and the second cell into a merging chamber of the electrofusion device by firing a third fluid dispenser of the electrofusion device, in response to alignment of the first cell in the first microfluidic channel with the second cell in the second microfluidic channel. The method further includes fusing the first cell and the second cell in the merging chamber by creating an electrical field in the merging chamber.

公开(公告)号：US20230123792A1

公开(公告)日：2023-04-20

申请号：US17910130

申请日：2020-03-31

Applicant: Hewlett-Packard Development Company, L.P.

Inventor： Alexander Govyadinov ,  Dennis M. LAZAROFF ,  Brett E. DAHLGREN ,  Stefan Enr LUCCHINI ,  Tod WOODFORD

IPC: B01L3/00 ,  G01N21/64

Abstract: The present disclosure is drawn to microfluidic devices. The microfluidic device includes a microfluidic well, a layered composite stack, and an optical sensor. The layered composite stack includes an optical filter composited with an etch-stopping layer. The optical filter defines the microfluidic well. The optical sensor is associated with the microfluidic well and has the optical filter positioned therebetween.

公开(公告)号：US20220362992A1

公开(公告)日：2022-11-17

申请号：US17878616

申请日：2022-08-01

Applicant: Hewlett-Packard Development Company, L.P.

Inventor： James Elmer Abbott, JR. ,  Alexander Govyadinov ,  Vladek Kasperchik ,  Krzysztof Nauka ,  Sivapackia Ganapathiappan ,  Lihua Zhao ,  Howard S. Tom ,  Yan Zhao ,  Hou T. Ng

IPC: B29C64/165 ,  B33Y30/00 ,  B29C64/291 ,  B29C64/277 ,  B29C64/264 ,  B29C64/268 ,  B29C35/08 ,  B29C67/00

Abstract: In one example, a lighting device for an additive manufacturing machine includes first light sources each to emit monochromatic light within a first band of wavelengths that includes a peak light absorption of a liquid coalescing agent and second light sources each to emit monochromatic light within a second band of wavelengths different from the first band of wavelengths. Each of the first light sources or each of multiple groups of the first light sources is individually addressable to emit monochromatic light independent of any other of the first light sources or of any other group of the first light sources and each of the second light sources or each of multiple groups of the second light sources is individually addressable to emit monochromatic light independent of any other of the second light sources or of any other group of the second light sources.

公开(公告)号：US20220184610A1

公开(公告)日：2022-06-16

申请号：US17416565

申请日：2019-07-25

Applicant: Hewlett-Packard Development Company, L.P.

Inventor： Alexander Govyadinov ,  Viktor Shkolnikov ,  Diane R. Hammerstad

IPC: B01L3/00

Abstract: In example implementations, an apparatus is provided. The apparatus includes a channel, an energy source, and a transfection chamber. The channel includes an indentation to hold a cell. The energy source is to apply a shockwave to the cell in the channel to porate the cell. The transfection chamber is to store a reagent to be inserted into the cell after the cell is porated.

公开(公告)号：US20220161568A1

公开(公告)日：2022-05-26

申请号：US17312366

申请日：2019-07-31

Applicant: Hewlett-Packard Development Company, L.P.

Inventor： Ronald Albert Askeland ,  Blair A. Butler ,  Ronald J. Ender ,  Craig L. Malik ,  Daniel D. Dowell ,  Seth Stephen Haddix ,  Si-lam J. Choy ,  Alexander Govyadinov

IPC: B41J2/175

Abstract: An example printing fluid pen comprises a plurality of fluid ports, a pressure regulator in fluid communication with a first fluid port, and a valve in fluid communication with a second fluid port. The first fluid port is to deliver printing fluid to a fluid ejection device, and the second fluid port to direct printing fluid out of the pen. In response to negative pressure, the valve is to open to enable fluids within the pen to exit via the second port.

公开(公告)号：US20220146383A1

公开(公告)日：2022-05-12

申请号：US17417811

申请日：2019-07-26

Applicant: Hewlett-Packard Development Company, L.P.

Inventor： Alexander Govyadinov ,  Viktor Shkolnikov

IPC: G01N1/38 ,  B01L3/00 ,  C12Q1/6825 ,  G01N33/58

Abstract: The present disclosure is drawn to a method of labeling and identifying microfluidic samples. The method can include tagging a first microfluidic sample with a first combination of markers to obtain a first labeled sample; tagging a second microfluidic sample with a second combination of markers that is different than the first combination of markers to obtain a second labeled sample; introducing a common variable to the first labeled sample and the second labeled sample. The common variable can generates a first interaction with the first labeled sample that is different than a second interaction or lack of interaction with the second labeled sample. The method can further include based on the first interaction, identifying the first labeled sample by assaying for the first combination of markers.

公开(公告)号：US20220062901A1

公开(公告)日：2022-03-03

申请号：US17459997

申请日：2021-08-27

Applicant: HEWLETT-PACKARD DEVELOPMENT COMPANY, L.P.

Inventor： Brian J. Keefe ,  Alexander Govyadinov ,  Jae Elliot De Leon ,  Braden Thomas Andrews ,  David S. Clague

IPC: B01L3/00

Abstract: In one example in accordance with the present disclosure, a fluid manipulation system is described. The fluid manipulation system includes a microfluidic channel through which fluid is to flow. The fluid includes particles to be separated. The fluid manipulation system includes a first electrode pair on a first side of the microfluidic channel. The first electrode pair includes a top electrode formed on a lid of the microfluidic channel and a bottom electrode formed on a floor of the microfluidic channel. The fluid manipulation system also includes a second electrode pair on a second side of the microfluidic channel. The second electrode pair also includes a top electrode and a bottom electrode. The electrode pairs are to generate an alternating electrical field across the microfluidic channel.