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    MICROORGANISMS AND METHODS FOR THE PRODUCTION OF 1,4-CYCLOHEXANEDIMETHANOL
    71.
    发明申请
    MICROORGANISMS AND METHODS FOR THE PRODUCTION OF 1,4-CYCLOHEXANEDIMETHANOL 审中-公开
    用于生产1,4-环己烷二甲醇的微生物和方法

    公开(公告)号：US20120156740A1

    公开(公告)日：2012-06-21

    申请号：US13327385

    申请日：2011-12-15

    申请人： Priti PHARKYA ,  Mark J. Burk

    发明人： Priti PHARKYA ,  Mark J. Burk

    IPC分类号： C12P7/02 ,  C12N1/19 ,  C12N1/15 ,  C12N1/21

    CPC分类号： C12P7/02 ,  C12N15/52 ,  C12N15/70 ,  C12N15/74 ,  C12N15/75 ,  C12N15/76 ,  C12N15/78 ,  C12N15/81 ,  C12N15/815 ,  C12P7/18

    摘要： A non-naturally occurring microbial organism includes a microbial organism having a 1,4-cyclohexanedimethanol pathway that includes at least one exogenous nucleic acid encoding a 1,4-cyclohexanedimethanol pathway enzyme expressed in a sufficient amount to produce 1,4-cyclohexanedimethanol. A method for producing 1,4-cyclohexanedimethanol includes culturing a non-naturally occurring microbial organism having a 1,4-cyclohexanedimethanol pathway. The pathway includes at least one exogenous nucleic acid encoding a 1,4-cyclohexanedimethanol pathway enzyme expressed in a sufficient amount to produce 1,4-cyclohexanedimethanol, under conditions and for a sufficient period of time to produce 1,4-cyclohexanedimethanol.

    摘要翻译： 非天然存在的微生物体包括具有1,4-环己烷二甲醇途径的微生物生物体，其包含至少一种编码1,4-环己烷二甲醇途径酶的外源核酸，其以足以产生1,4-环己烷二甲醇的量表达。 生产1,4-环己烷二甲醇的方法包括培养具有1,4-环己烷二甲醇途径的非天然存在的微生物。 该途径包括至少一种编码1,4-环己烷二甲醇途径酶的外源核酸，其在足以在足够的时间内产生1,4-环己烷二甲醇以足够量产生1,4-环己烷二甲醇。

    METHODS FOR THE SYNTHESIS OF OLEFINS AND DERIVATIVES
    74.
    发明申请
    METHODS FOR THE SYNTHESIS OF OLEFINS AND DERIVATIVES 有权
    烯烃和衍生物的合成方法

    公开(公告)号：US20120094341A1

    公开(公告)日：2012-04-19

    申请号：US13219423

    申请日：2011-08-26

    申请人： Mark J. Burk ,  Priti Pharkya ,  Stephen J. Van Dien ,  Anthony P. Burgard ,  Christophe H. Schilling

    发明人： Mark J. Burk ,  Priti Pharkya ,  Stephen J. Van Dien ,  Anthony P. Burgard ,  Christophe H. Schilling

    IPC分类号： C12P7/40 ,  C07C51/347

    CPC分类号： C07C51/353 ,  C07C51/38 ,  C07C57/04 ,  C07C67/08 ,  C07C67/32 ,  C07C67/333 ,  C12P7/40 ,  C12P7/46 ,  C12P7/62 ,  Y02P20/125 ,  C07C69/60 ,  C07C69/54

    摘要： The invention provides a method of producing acrylic acid. The method includes contacting fumaric acid with a sufficient amount of ethylene in the presence of a cross-metathesis transformation catalyst to produce about two moles of acrylic acid per mole of fumaric acid. Also provided is an acrylate ester. The method includes contacting fumarate diester with a sufficient amount of ethylene in the presence of a cross-metathesis transformation catalyst to produce about two moles of acrylate ester per mole of fumarate diester. An integrated process for process for producing acrylic acid or acrylate ester is provided which couples bioproduction of fumaric acid with metathesis transformation. An acrylic acid and an acrylate ester production also is provided.

    摘要翻译： 本发明提供了制备丙烯酸的方法。 该方法包括在交叉复分解转化催化剂存在下使富马酸与足够量的乙烯接触，以产生约2摩尔丙烯酸/摩尔富马酸。 还提供了丙烯酸酯。 该方法包括在交叉复分解转化催化剂存在下使富马酸二酯与足够量的乙烯接触，以产生每摩尔富马酸二酯约2摩尔丙烯酸酯。 提供了用于生产丙烯酸或丙烯酸酯的方法的综合方法，其将富马酸的生物生产与易位转化相结合。 还提供丙烯酸和丙烯酸酯生产。

    Chemoenzymatic methods for the synthesis of statins and statin intermediates
    75.
    发明授权
    Chemoenzymatic methods for the synthesis of statins and statin intermediates 有权
    用于合成他汀类药物和他汀类药物中间体的化疗酶法

    公开(公告)号：US08148324B2

    公开(公告)日：2012-04-03

    申请号：US12032337

    申请日：2008-02-15

    申请人： William Greenberg ,  Mark J. Burk ,  Alexander Varvak ,  Kelvin Wong

    发明人： William Greenberg ,  Mark J. Burk ,  Alexander Varvak ,  Kelvin Wong

    IPC分类号： C07K14/00 ,  C12P21/00

    CPC分类号： C12P13/004 ,  C12N9/88 ,  C12P7/42 ,  C12P17/02 ,  C12P17/06 ,  C12P17/10 ,  C12P17/12

    摘要： The invention provides aldolases, nucleic acids encoding them and methods for making and using them, including chemoenzymatic processes for making β,δ-dihydroxyheptanoic acid side chains and compositions comprising these side chains, e.g., [R—(R*,R*)]-2-(4-fluorophenyl)-b,d-dihydroxy-5-(1-methylethyl)-3-phenyl-4-(phenylamino)-carbonyl]-1H-pyrrole-1-heptanoic acid (atorvastatin, LIPITOR™), rosuvastatin (CRESTOR™), fluvastatin (LESCOL™), related compounds and their intermediates.

    摘要翻译： 本发明提供醛缩酶，编码它们的核酸和制备和使用它们的方法，包括制备和/或制备δ-二羟基庚酸侧链的化学方法和包含这些侧链的组合物，例如[R-（R *，R *） ] -2-（4-氟苯基）-b，d-二羟基-5-（1-甲基乙基）-3-苯基-4-（苯基氨基） - 羰基] -1H-吡咯-1-庚酸（阿托伐他汀，LIPITOR TM ），瑞舒伐他汀（CRESTOR TM），氟伐他汀（LESCOL TM），相关化合物及其中间体。

    Compositions and methods for the biosynthesis of 1,4-butanediol and its precursors
    77.
    发明授权
    Compositions and methods for the biosynthesis of 1,4-butanediol and its precursors 有权
    1,4-丁二醇及其前体的生物合成的组合物和方法

    公开(公告)号：US08067214B2

    公开(公告)日：2011-11-29

    申请号：US12049256

    申请日：2008-03-14

    申请人： Mark J. Burk ,  Stephen J. Van Dien ,  Anthony P. Burgard ,  Wei Niu

    发明人： Mark J. Burk ,  Stephen J. Van Dien ,  Anthony P. Burgard ,  Wei Niu

    IPC分类号： C12P7/18 ,  C12N1/21

    CPC分类号： C12N15/52 ,  B01D3/002 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/88 ,  C12N9/93 ,  C12N15/70 ,  C12N15/81 ,  C12P7/18 ,  C12P7/42 ,  C12P7/52 ,  C12P17/04 ,  C12Y101/01061 ,  C12Y102/01076 ,  C12Y401/01071 ,  C12Y602/01004 ,  Y02P20/52

    摘要： The invention provides a non-naturally occurring microbial organism having 4-hydroxybutanoic acid (4-HB) and 1,4-butanediol (1,4-BDO) biosynthetic pathways. The pathways include exogenous nucleic acids encoding a) an α-ketoglutarate decarboxylase; b) a 4-hydroxybutanoate dehydrogenase; c) a 4-hydroxybutyryl-CoA:acetyl-CoA transferase or a butyrate kinase and a phosphotransbutyrylase; d) an aldehyde dehydrogenase, and e) an alcohol dehydrogenase, wherein the exogenous nucleic acids are expressed in sufficient amounts to produce 1,4-butanediol (1,4-BDO). Also provide is a method for the production of 1,4-BDO. The method includes culturing the non-naturally occurring microbial organism having 4-HB and 1,4-BDO biosynthetic pathways substantially anaerobic conditions for a sufficient period of time to produce 1,4-BDO.

    摘要翻译： 本发明提供了具有4-羟基丁酸（4-HB）和1,4-丁二醇（1,4-BDO）生物合成途径的非天然存在的微生物生物。 途径包括编码a）α-酮戊二酸脱羧酶的外源核酸; b）4-羟基丁酸脱氢酶; c）4-羟基丁酰-CoA：乙酰-CoA转移酶或丁酸酯激酶和磷酸转磷酸化酶; d）醛脱氢酶，和e）醇脱氢酶，其中外源核酸以足够的量表达以产生1,4-丁二醇（1,4-BDO）。 还提供了用于生产1,4-BDO的方法。 该方法包括培养具有4-HB和1,4-BDO生物合成途径的非天然存在的微生物生物合成途径，基本上是厌氧条件足够的时间以产生1,4-BDO。

    Methods for the synthesis of olefins and derivatives
    78.
    发明授权
    Methods for the synthesis of olefins and derivatives 有权
    合成烯烃和衍生物的方法

    公开(公告)号：US08026386B2

    公开(公告)日：2011-09-27

    申请号：US12188582

    申请日：2008-08-08

    申请人： Mark J. Burk ,  Priti Pharkya ,  Stephen J. Van Dien ,  Anthony P. Burgard ,  Christophe H. Schilling

    发明人： Mark J. Burk ,  Priti Pharkya ,  Stephen J. Van Dien ,  Anthony P. Burgard ,  Christophe H. Schilling

    IPC分类号： C07C67/00 ,  C07C69/52 ,  C12P7/40

    CPC分类号： C07C51/353 ,  C07C51/38 ,  C07C57/04 ,  C07C67/08 ,  C07C67/32 ,  C07C67/333 ,  C12P7/40 ,  C12P7/46 ,  C12P7/62 ,  Y02P20/125 ,  C07C69/60 ,  C07C69/54

    摘要： The invention provides a method of producing acrylic acid. The method includes contacting fumaric acid with a sufficient amount of ethylene in the presence of a cross-metathesis transformation catalyst to produce about two moles of acrylic acid per mole of fumaric acid. Also provided is an acrylate ester. The method includes contacting fumarate diester with a sufficient amount of ethylene in the presence of a cross-metathesis transformation catalyst to produce about two moles of acrylate ester per mole of fumarate diester. An integrated process for process for producing acrylic acid or acrylate ester is provided which couples bioproduction of fumaric acid with metathesis transformation. An acrylic acid and an acrylate ester production also is provided.

    摘要翻译： 本发明提供了制备丙烯酸的方法。 该方法包括在交叉复分解转化催化剂存在下使富马酸与足够量的乙烯接触，以产生约2摩尔丙烯酸/摩尔富马酸。 还提供了丙烯酸酯。 该方法包括在交叉复分解转化催化剂存在下使富马酸二酯与足够量的乙烯接触，以产生每摩尔富马酸二酯约2摩尔丙烯酸酯。 提供了用于生产丙烯酸或丙烯酸酯的方法的综合方法，其将富马酸的生物生产与易位转化相结合。 还提供丙烯酸和丙烯酸酯生产。

    METHODS AND ORGANISMS FOR UTILIZING SYNTHESIS GAS OR OTHER GASEOUS CARBON SOURCES AND METHANOL
    79.
    发明申请
    METHODS AND ORGANISMS FOR UTILIZING SYNTHESIS GAS OR OTHER GASEOUS CARBON SOURCES AND METHANOL 有权
    使用合成气或其他气态碳源和甲醇的方法和有机体

    公开(公告)号：US20110223637A1

    公开(公告)日：2011-09-15

    申请号：US13106764

    申请日：2011-05-12

    申请人： Mark J. Burk ,  Christophe H. Schilling ,  Anthony P. Burgard ,  John D. Trawick

    发明人： Mark J. Burk ,  Christophe H. Schilling ,  Anthony P. Burgard ,  John D. Trawick

    IPC分类号： C12P19/40 ,  C12N1/00

    CPC分类号： C12P7/18 ,  C12N9/0008 ,  C12N9/0036 ,  C12N9/1007 ,  C12N9/93 ,  C12N15/70 ,  C12P19/32 ,  Y02E50/343

    摘要： The invention provides a non-naturally occurring microbial organism having an acetyl-CoA pathway and the capability of utilizing syngas or syngas and methanol. In one embodiment, the invention provides a non-naturally occurring microorganism, comprising one or more exogenous proteins conferring to the microorganism a pathway to convert CO, CO2 and/or H2 to acetyl-coenzyme A (acetyl-CoA), methyl tetrahydrofolate (methyl-THF) or other desired products, wherein the microorganism lacks the ability to convert CO or CO2 and H2 to acetyl-CoA or methyl-THF in the absence of the one or more exogenous proteins. For example, the microbial organism can contain at least one exogenous nucleic acid encoding an enzyme or protein in an acetyl-CoA pathway. The microbial organism is capable of utilizing synthesis gases comprising CO, CO2 and/or H2, alone or in combination with methanol, to produce acetyl-CoA. The invention additionally provides a method for producing acetyl-CoA, for example, by culturing an acetyl-CoA producing microbial organism, where the microbial organism expresses at least one exogenous nucleic acid encoding an acetyl-CoA pathway enzyme or protein in a sufficient amount to produce acetyl-CoA, under conditions and for a sufficient period of time to produce acetyl-CoA.

    摘要翻译： 本发明提供具有乙酰-CoA途径的非天然存在的微生物生物体，以及利用合成气或合成气和甲醇的能力。 在一个实施方案中，本发明提供非天然存在的微生物，其包含一种或多种外源蛋白质，赋予微生物将CO，CO 2和/或H 2转化为乙酰辅酶A（乙酰辅酶A），甲基四氢叶酸（甲基 -THF）或其它所需产物，其中微生物缺乏在不存在一种或多种外源蛋白的情况下将CO或CO 2和H 2转化为乙酰辅酶A或甲基-THF的能力。 例如，微生物生物体可以含有至少一种编码乙酰辅酶A途径中的酶或蛋白质的外源核酸。 微生物生物能够单独或与甲醇组合使用包含CO，CO 2和/或H 2的合成气体，以产生乙酰辅酶A。 本发明另外提供了生产乙酰辅酶A的方法，例如通过培养产生乙酰辅酶A的微生物，其中微生物生物体表达至少一种编码乙酰辅酶A途径酶或蛋白质的外源核酸，其量足够 在条件下和足够的时间内产生乙酰辅酶A产生乙酰辅酶A。