利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

89 条记录 (耗时 0.05 秒)

    METHODS OF NUCLEIC ACID IDENTIFICATION IN LARGE-SCALE SEQUENCING
    71.
    发明申请
    METHODS OF NUCLEIC ACID IDENTIFICATION IN LARGE-SCALE SEQUENCING 审中-公开
    大规模序列中核酸鉴定方法

    公开(公告)号：US20090105961A1

    公开(公告)日：2009-04-23

    申请号：US11938213

    申请日：2007-11-09

    申请人： Radoje Drmanac

    发明人： Radoje Drmanac

    IPC分类号： G01N33/48

    CPC分类号： C12Q1/6874 ,  G16B30/00

    摘要： The present invention provides methods for determining a base probability in a target nucleic acid within an experimental data set. The methods of the invention provide specific methods of improving accuracy of base calling for experimental sequencing data compared to conventional methods. The experimental base values used in the methods of the present invention provide relative base probabilities within an experimental data set that are robust and uniformly optimal regardless of the experimental conditions.

    摘要翻译： 本发明提供了用于确定实验数据集内靶核酸的基本概率的方法。 与传统方法相比，本发明的方法提供了提高实验测序数据的碱基调用准确性的具体方法。 在本发明的方法中使用的实验基础值提供实验数据集内的相对基本概率，无论实验条件如何，鲁棒性和均匀最佳。

    METHODS FOR COMPUTING POSITIONAL BASE PROBABILITIES USING EXPERMINENTALS BASE VALUE DISTRIBUTIONS
    72.
    发明申请
    METHODS FOR COMPUTING POSITIONAL BASE PROBABILITIES USING EXPERMINENTALS BASE VALUE DISTRIBUTIONS 审中-公开
    使用现金基数值分配计算位置概率的方法

    公开(公告)号：US20080221832A1

    公开(公告)日：2008-09-11

    申请号：US11938221

    申请日：2007-11-09

    申请人： Radoje Drmanac

    发明人： Radoje Drmanac

    IPC分类号： G06F17/18

    CPC分类号： C12Q1/6874 ,  G16B30/00

    摘要： Aspects of the various embodiments of the invention relate generally to computing relative base value probabilities using discrete experimental base values to calculate distributions of relative base probabilities. This information can be used with associated experimental measurements to increase the accuracy of the data analysis.

    摘要翻译： 本发明的各个实施例的方面一般涉及使用离散实验基值计算相对基值概率来计算相对基本概率的分布。 该信息可以与相关的实验测量一起使用，以提高数据分析的准确性。

    Methods and compositions for efficient nucleic acid sequencing
    75.
    发明授权
    Methods and compositions for efficient nucleic acid sequencing 失效
    用于有效核酸测序的方法和组合物

    公开(公告)号：US06401267B1

    公开(公告)日：2002-06-11

    申请号：US09272232

    申请日：1999-03-18

    申请人： Radoje Drmanac

    发明人： Radoje Drmanac

    IPC分类号： C12Q168

    CPC分类号： C12Q1/6874

    摘要： Disclosed are novel methods and compositions for rapid and highly efficient nucleic acid sequencing based upon hybridization with two sets of small oligonucleotide probes of known sequences. Extremely large nucleic acid molecules, including chromosomes and non-amplified RNA, may be sequenced without prior cloning or subcloning steps. The methods of the invention also solve various current problems associated with sequencing technology such as, for example, high noise to signal ratios and difficult discrimination, attaching many nucleic acid fragments to a surface, preparing many, longer or more complex probes and labelling more species.

    摘要翻译： 公开了基于与已知序列的两组小寡核苷酸探针杂交的用于快速和高效核酸测序的新颖方法和组合物。 非常大的核酸分子，包括染色体和非扩增的RNA，可以在没有先前的克隆或亚克隆步骤的情况下进行测序。 本发明的方法还解决了与排序技术相关的各种当前问题，例如高噪声与信号比和困难的鉴别，将许多核酸片段连接到表面，制备许多更长或更复杂的探针并标记更多的物质 。

    Nucleic acid sequence analysis from combined mixtures of amplified fragments
    76.
    发明授权
    Nucleic acid sequence analysis from combined mixtures of amplified fragments 有权
    来自扩增片段的组合混合物的核酸序列分析

    公开(公告)号：US08765379B2

    公开(公告)日：2014-07-01

    申请号：US13017244

    申请日：2011-01-31

    申请人： Radoje Drmanac

    发明人： Radoje Drmanac

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6874 ,  C07H21/04 ,  C07K1/047 ,  C12Q1/6806 ,  C12Q1/682 ,  C12Q1/6837 ,  C12Q1/6869 ,  C12Q2525/151 ,  C12Q2525/313 ,  C12Q2531/125 ,  C12Q2565/513 ,  G01N15/1404 ,  G01N15/1434 ,  Y10S977/778 ,  Y10S977/789 ,  Y10S977/792 ,  Y10S977/88 ,  Y10S977/882 ,  C12Q2521/307 ,  C12Q2525/161 ,  C12Q2535/122 ,  C12Q2563/179 ,  C12Q2565/514

    摘要： The invention provides methods and kits for ordering sequence information derived from one or more target polynucleotides. In one aspect, one or more tiers or levels of fragmentation and aliquoting are generated, after which sequence information is obtained from fragments in a final level or tier. Each fragment in such final tier is from a particular aliquot, which, in turn, is from a particular aliquot of a prior tier, and so on. For every fragment of an aliquot in the final tier, the aliquots from which it was derived at every prior tier is known, or can be discerned. Thus, identical sequences from overlapping fragments from different aliquots can be distinguished and grouped as being derived from the same or different fragments from prior tiers. When the fragments in the final tier are sequenced, overlapping sequence regions of fragments in different aliquots are used to register the fragments so that non-overlapping regions are ordered. In one aspect, this process is carried out in a hierarchical fashion until the one or more target polynucleotides are characterized, e.g. by their nucleic acid sequences, or by an ordering of sequence segments, or by an ordering of single nucleotide polymorphisms (SNPs), or the like.

    摘要翻译： 本发明提供了用于排序从一个或多个目标多核苷酸衍生的序列信息的方法和试剂盒。 在一个方面，产生一个或多个分层或等级的碎片和等分试样，之后从最终级别或层级的片段获得序列信息。 这样的最后一层中的每个片段都来自特定的等分试样，而这些等分试样又是来自先前层的特定等分试样，等等。 对于最后一层中的等分试样的每个片段，从每个先前的层次派生的等分试样是已知的，或者可以被辨别出来。 因此，来自不同等分试样的重叠片段的相同序列可以被区分并分组为从与先前层相同或不同的片段衍生的。 当最终层中的片段被排序时，使用不同等分试样的片段的重叠序列区域来登记片段，使得非重叠区域被排序。 在一个方面，该方法以分级方式进行，直到一个或多个目标多核苷酸被表征为例如。 通过其核酸序列，或通过序列片段的排序，或通过单核苷酸多态性（SNP）等的排序。

    METHOD FOR NUCLEIC ACID DETECTION USING VOLTAGE ENHANCEMENT
    78.
    发明申请
    METHOD FOR NUCLEIC ACID DETECTION USING VOLTAGE ENHANCEMENT 有权
    使用电压增强的核酸检测方法

    公开(公告)号：US20120122721A1

    公开(公告)日：2012-05-17

    申请号：US13337968

    申请日：2011-12-27

    申请人： Andres Fernandez ,  Bryan Staker ,  Radoje Drmanac

    发明人： Andres Fernandez ,  Bryan Staker ,  Radoje Drmanac

    IPC分类号： C40B30/04

    CPC分类号： C12Q1/6832 ,  C12Q1/6825 ,  C12Q2523/307 ,  C12Q2565/501 ,  C12Q2565/607

    摘要： Methods are provided for carrying out nucleic acid analysis, including sequence identification, employing voltage and/or controlled electric charge to enhance operation. A device comprises substrates for nucleic acid analysis, a first electrically conductive layer, a first electrically insulative layer of dielectric material on the first conductive layer, a second electrically conductive layer disposed upon the first insulative layer in a pattern to define discrete attachment sites for macromolecules on the first insulative layer, the second conductive layer provided with means for resisting affinity for the macromolecules to impede their attachment to sites on the second conductive layer, and terminals for the first and second conductive layers for applying a voltage pattern between the first and the second conductive layers to control affinity between the macromolecules and the discrete attachment sites.

    摘要翻译： 提供用于进行核酸分析的方法，包括序列鉴定，采用电压和/或受控电荷以增强操作。 一种器件包括用于核酸分析的衬底，第一导电层，第一导电层上的介电材料的第一电绝缘层，第二导电层，以图案形式设置在第一绝缘层上，以限定大分子的离散附着位点 在第一绝缘层上，第二导电层设置有用于抵抗大分子阻挡其附着于第二导电层上的部位的亲和力的装置，以及用于在第一和第二绝缘层之间施加电压图案的第一和第二导电层的端子 第二导电层以控制大分子和离散附着位点之间的亲和力。