公开(公告)号：US20050136504A1

公开(公告)日：2005-06-23

申请号：US11050763

申请日：2005-02-07

申请人： Masashi Ogawa ,  Tsutomo Hamaoka ,  Yutaka Tamura ,  Tetsuhiko Tachikawa ,  Ikuo Hasegawa ,  Shusaku Yoshiki ,  Kohji Hasegawa ,  Junji Nishigaki

发明人： Masashi Ogawa ,  Tsutomo Hamaoka ,  Yutaka Tamura ,  Tetsuhiko Tachikawa ,  Ikuo Hasegawa ,  Shusaku Yoshiki ,  Kohji Hasegawa ,  Junji Nishigaki

IPC分类号： C12Q1/37

CPC分类号： C12Q1/37 ,  G01N2333/78 ,  G01N2333/96486

摘要： A method for measuring protease such as matrix metalloproteinase which comprising steps of (1) bringing a biological sample such as protease-containing tissue slices and tissue extracts into contact with a thin membrane which comprises a protease substrate and a hardening agent and is formed on a surface of a support; and (2) detecting the trace of digestion formed on the thin membrane by the action of protease with the naked eyes or under a microscope, and a thin membrane used for said method.

摘要翻译： 一种测定蛋白酶如基质金属蛋白酶的方法，包括以下步骤：（1）将含有蛋白酶的组织切片和组织提取物的生物样品与包含蛋白酶底物和硬化剂的薄膜接触，并形成在 支撑面; 和（2）用肉眼或显微镜检测蛋白酶作用下薄膜上形成的消化痕迹，以及用于所述方法的薄膜。

公开(公告)号：US06864055B2

公开(公告)日：2005-03-08

申请号：US09887625

申请日：2001-06-22

申请人： Yoshihiko Makino ,  Yoshihiko Abe ,  Masashi Ogawa ,  Makoto Takagi ,  Shigeori Takenaka ,  Kenichi Yamashita

发明人： Yoshihiko Makino ,  Yoshihiko Abe ,  Masashi Ogawa ,  Makoto Takagi ,  Shigeori Takenaka ,  Kenichi Yamashita

IPC分类号： G01N33/53 ,  C07B61/00 ,  C07K14/00 ,  C12M1/00 ,  C12N15/09 ,  C12Q1/68 ,  G01N27/416 ,  G01N33/566 ,  B01D57/02 ,  C12P19/34 ,  G01N30/02

CPC分类号： C12Q1/6825 ,  C07K14/003 ,  C12Q1/6837 ,  C40B40/00 ,  C12Q2565/501 ,  C12Q2565/102 ,  C12Q2565/607

摘要： A method of detecting nucleic acid fragments in plural samples is performed by the steps of: attaching an electroconductive label to nucleic acid fragments in one sample and attaching a different electroconductive label to nucleic acid fragments in another sample; preparing a mixture of these samples; spotting the mixture on an electroconductive microarray having plural electrodes onto which probe molecules complementary to the nucleic acid fragments are fixed, so that hybridization between the nucleic acid fragments and the probe molecules on the electroconductive microarray can proceed to form hybrid structures; applying to the electrode an electric potential corresponding to the oxidation-reduction potential of the former label and detecting on the electrode an electric current; applying to the electrode an electric potential corresponding to the oxidation-reduction potential of the latter label and detecting on the electrode an electric current; and comparing the electric current detected in the former detecting procedure and that detected in the latter detecting procedure.

摘要翻译： 通过以下步骤进行检测多个样品中的核酸片段的方法：将导电标记物附着在一个样品中的核酸片段上，并将不同的导电标记连接到另一个样品中的核酸片段; 准备这些样品的混合物; 在具有多个电极的导电微阵列上将该混合物点样，在该导电微阵列上固定有与核酸片段互补的探针分子，使得导电微阵列上的核酸片段和探针分子之间的杂交可以进行以形成杂化结构; 向所述电极施加与所述前标签的氧化还原电位相对应的电位，并在所述电极上检测电流; 向所述电极施加与后一标签的氧化还原电位相对应的电位，并在所述电极上检测电流; 并比较前一检测程序检测的电流和后一检测程序中检测的电流。

公开(公告)号：US06713255B1

公开(公告)日：2004-03-30

申请号：US09589221

申请日：2000-06-07

申请人： Yoshihiko Makino ,  Yoshihiko Abe ,  Makoto Takagi ,  Shigeori Takenaka ,  Kenichi Yamashita ,  Masashi Ogawa

发明人： Yoshihiko Makino ,  Yoshihiko Abe ,  Makoto Takagi ,  Shigeori Takenaka ,  Kenichi Yamashita ,  Masashi Ogawa

IPC分类号： C12Q168

CPC分类号： C07K14/003 ,  B01J19/0046 ,  B01J2219/00529 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00608 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00617 ,  B01J2219/00626 ,  B01J2219/00637 ,  B01J2219/00641 ,  B01J2219/00659 ,  B01J2219/00722 ,  B01J2219/00729 ,  C07B2200/11 ,  C07H21/00 ,  C12Q1/6837 ,  C40B40/06 ,  C12Q2565/607

摘要： A DNA chip (or PNA chip) composed of a solid carrier and plural DNA fragments (or PNA fragments) fixed onto is the solid carrier at each one end, wherein a plurality of short chain spacer molecules having a hydrophilic moiety at each one end are fixed at each another end onto a surface of the solid carrier having no DNA fragments (or no PNA fragments) on its surface is effective for high sensitive quantitative analysis of a nucleic acid fragment complementary to the DNA fragment (or PNA fragment).

摘要翻译： 由固体载体和固定在其上的多个DNA片段（或PNA片段）组成的DNA芯片（或PNA芯片）是每一端的固体载体，其中在每一端具有亲水部分的多个短链间隔基分子是 固定在其表面上没有DNA片段（或没有PNA片段）的固体载体的表面上对于与DNA片段（或PNA片段）互补的核酸片段的高敏感性定量分析是有效的。

公开(公告)号：US06652070B2

公开(公告)日：2003-11-25

申请号：US10214107

申请日：2002-08-08

申请人： Kiyomi Aono ,  Ken Hosaka ,  Kaneji Yamada ,  Masashi Ogawa

发明人： Kiyomi Aono ,  Ken Hosaka ,  Kaneji Yamada ,  Masashi Ogawa

IPC分类号： B41J214

CPC分类号： B41J2/04508 ,  B41J2/0458 ,  B41J2/04593 ,  B41J2/14056 ,  B41J2/16526 ,  B41J2002/14379

摘要： An jet recording apparatus, which records on a recording medium by discharging ink form a recording head, is provided with at least two electrothermal converting elements each having different amount of heat generation, and driven each individually, which are arranged in the ink flow path communicated with the discharge port of the recording head along the ink flow path each in different distance to the discharge port, and when the pre-discharge is performed without participating in recording, driving signals needed for discharging ink are supplied to the electrothermal converting element on the side near to the discharge port subsequent to being supplied to the electrothermal converting element on the side away from the discharge port. The pre-discharge is performed immediately before recording per line, and the number of discharges is set to be larger for the electrothermal converting element on the side away from the discharge port than the number of discharges for the electrothermal converting element on the side near to the discharge port.

公开(公告)号：US6115562A

公开(公告)日：2000-09-05

申请号：US269194

申请日：1999-03-30

申请人： Masashi Ogawa ,  Hisanobu Matsuzoe ,  Kouji Migita ,  Yasunori Sagara ,  Kazuo Hakukawa ,  Shinichi Kizu ,  Yusuke Shiibara ,  Yukinori Hara

发明人： Masashi Ogawa ,  Hisanobu Matsuzoe ,  Kouji Migita ,  Yasunori Sagara ,  Kazuo Hakukawa ,  Shinichi Kizu ,  Yusuke Shiibara ,  Yukinori Hara

IPC分类号： G03G15/16

CPC分类号： G03G15/167 ,  G03G2215/00974

摘要： A toner image which is carried by a photosensitive endless belt is pressed against a printing paper by a transfer opposed roller provided inside the photosensitive belt to transfer the toner image onto the printing paper, and the image transferred onto the printing paper is fixed. The position of the transfer roller which is moved inside the photosensitive belt is detected by a detector. When the detected position agrees with a predetermined position, the moving direction is reversed by a control unit and the moving distance of the transfer roller is varied in accordance with the size of the image ad, further, the contamination of the backside of the printing paper is avoided.

摘要翻译： PCT No.PCT / JP97 / 03463。 371日期1999年3月30日 102（e）1999年3月30日PCT 1997年9月29日PCT公布。 WO98 / 37460 PCT公开号 日期1998年8月27日由感光环形带承载的调色剂图像通过设置在感光带内部的转印对置辊压在打印纸上，以将调色剂图像转印到打印纸上，并将图像转印到打印纸上 是固定的 通过检测器检测在感光带内移动的转印辊的位置。 当检测到的位置与预定位置一致时，通过控制单元使移动方向反转，并且转印辊的移动距离根据图像广告尺寸而变化，此外，打印纸背面的污染 被避免。

公开(公告)号：US6085057A

公开(公告)日：2000-07-04

申请号：US284385

申请日：1999-04-12

申请人： Hisanobu Matsuzoe ,  Yasunori Sagara ,  Masashi Ogawa ,  Yusuke Shiibara ,  Shinichi Kizu ,  Kouji Migita ,  Yukinori Hara

发明人： Hisanobu Matsuzoe ,  Yasunori Sagara ,  Masashi Ogawa ,  Yusuke Shiibara ,  Shinichi Kizu ,  Kouji Migita ,  Yukinori Hara

IPC分类号： G03G15/01 ,  G03G15/08 ,  G03G15/16

CPC分类号： G03G15/167 ,  G03G15/0163 ,  G03G2215/018

摘要： An image forming device and an image forming method for transporting a toner image on an endless belt shaped toner image transporting member. The toner image transporting member is supported by a plurality of rollers and is stretched out and made rotatable in the same direction as the recording medium is transported. The colored toner is transported on the outside of the toner image transporting member and is transferred onto stationary recording medium by an opposed image transferring roller while the rotation of the toner image transporting member is suspended. The image transferring process is repeated for the required number of colors.

摘要翻译： PCT No.PCT / JP97 / 03560 Sec。 371日期1999年4月12日 102（e）1999年4月12日PCT PCT 1997年10月6日PCT公布。 第WO98 / 34161号公报 日期1998年8月6日一种用于在环形带状调色剂图像传送部件上传送调色剂图像的图像形成装置和图像形成方法。 调色剂图像传送构件由多个辊支撑并且被拉伸并且沿与传送记录介质相同的方向可旋转。 彩色调色剂在调色剂图像传送构件的外侧传送，并且在调色剂图像传送构件的旋转被暂停时通过相对的图像转印辊转印到固定的记录介质上。 对所需数量的颜色重复图像转印处理。

公开(公告)号：US6044240A

公开(公告)日：2000-03-28

申请号：US50988

申请日：1998-03-31

申请人： Yasunori Sagara ,  Hisanobu Matsuzoe ,  Shinichi Kizu ,  Masashi Ogawa ,  Yusuke Shiibara ,  Kouji Migita ,  Yukinori Hara ,  Kazuo Hakukawa

发明人： Yasunori Sagara ,  Hisanobu Matsuzoe ,  Shinichi Kizu ,  Masashi Ogawa ,  Yusuke Shiibara ,  Kouji Migita ,  Yukinori Hara ,  Kazuo Hakukawa

IPC分类号： G03G15/16 ,  G03G15/00

CPC分类号： G03G15/167

摘要： A transverse-type image forming apparatus in which a toner image carried on an endless toner image carrying belt is transferred to a printing paper by a transfer facing roller provided inside the toner image carrying belt and caused to move along the printing paper. A transfer bias voltage is applied to a transfer plate, on which the printing paper is set, during the backward movement of, or during both the forward and backward movements of, the transfer facing roller, so as to cause the toner image to be electrostatically attracted to the printing paper. The uneven transfer of the toner image is reduced.

摘要翻译： 一种横向型图像形成装置，其中承载在无端调色剂图像承载带上的调色剂图像通过设置在调色剂图像承载带内部并且沿着打印纸移动的转印面向辊转印到打印纸。 在转印面向辊的向后移动或向前和向后移动期间，将转印偏压施加到其上设置有打印纸的转印板，以使调色剂图像静电 吸引到印刷纸上。 调色剂图像的不均匀转印减少。

公开(公告)号：US5983061A

公开(公告)日：1999-11-09

申请号：US97686

申请日：1998-06-16

申请人： Hisanobu Matsuzoe ,  Kouji Migita ,  Shinichi Kizu ,  Yusuke Shiibara ,  Yasunori Sagara ,  Masashi Ogawa ,  Yukinori Hara

发明人： Hisanobu Matsuzoe ,  Kouji Migita ,  Shinichi Kizu ,  Yusuke Shiibara ,  Yasunori Sagara ,  Masashi Ogawa ,  Yukinori Hara

IPC分类号： G03G15/00 ,  G03G15/16 ,  G03G21/00

CPC分类号： G03G15/167 ,  G03G2215/00974

摘要： A toner image onto a print form by pressing a photosensitive material belt against a transfer plate through movement of a transfer opposite roller. The photosensitive material belt running in a direction orthogonal to the supply direction of the print form on a transfer plate. The photosensitive material belt is stably maintained at a region completed for transfer in an attitude following the upper surface of the print form.

摘要翻译： 通过转印对面辊的移动将感光材料带压靠在转印板上，将调色剂图像转印到印刷版上。 感光材料带在转印板上沿与打印形式的供给方向正交的方向上行进。 感光材料带被稳定地保持在完成以在打印形式的上表面之后的状态下传送的区域。

公开(公告)号：US5811439A

公开(公告)日：1998-09-22

申请号：US768937

申请日：1996-12-18

申请人： Masashi Ogawa ,  Tadashi Morita ,  Norihiro Iibuchi ,  Hideyuki Tsutsui

发明人： Masashi Ogawa ,  Tadashi Morita ,  Norihiro Iibuchi ,  Hideyuki Tsutsui

IPC分类号： A61K31/425 ,  A61K31/44 ,  A61K31/475 ,  A61P3/06 ,  A61P3/08 ,  A61P3/10 ,  C07D417/06 ,  C07D417/14

CPC分类号： C07D417/06 ,  C07D417/14

摘要： A thiazolidinedione derivative represented by the following general formula (I): ##STR1## �wherein the dotted line represents a single bond or a double bond, the thiazolidinedione ring residue is linked to either of 2-, 3-, 4-, 5- and 6-positions on the indole ring and R represents a group selected from the group consisting of hydrogen atom and alkyl, alkenyl, alkynyl, phenyl, aralkyl, heterocycloalkyl, arylsulfonyl and arylaminocarbonyl groups! or a pharmaceutically acceptable salt thereof exhibits excellent effects of reducing the blood sugar level and of reducing the lipid concentration in blood and is accordingly useful as a therapeutic agent for treating diabates mellitus. These derivatives and pharmaceutically acceptable salt thereof are almost free of any side effect.

摘要翻译： 由下列通式（I）表示的噻唑烷二酮衍生物：其中虚线代表单键或双键，噻唑烷二酮环残基与2-，3-，4- 在吲哚环上的5-和6-位，R表示选自氢原子和烷基，烯基，炔基，苯基，芳烷基，杂环烷基，芳基磺酰基和芳基氨基羰基的基团]或其药学上可接受的盐显示出优异的 降低血糖水平和降低血液中的脂质浓度的效果，因此可用作治疗二倍体的治疗剂。 这些衍生物及其药学上可接受的盐几乎没有副作用。

公开(公告)号：US5447846A

公开(公告)日：1995-09-05

申请号：US91661

申请日：1993-07-14

申请人： Hiroshi Shinoki ,  Masashi Ogawa

发明人： Hiroshi Shinoki ,  Masashi Ogawa

IPC分类号： G01N33/542 ,  G01N33/543 ,  G01N33/58 ,  G01N33/68 ,  G01N33/53

CPC分类号： G01N33/581 ,  G01N33/542 ,  G01N33/54386 ,  G01N33/6857 ,  Y10S435/964 ,  Y10S435/969 ,  Y10S435/97 ,  Y10S435/972 ,  Y10S530/866

摘要： An enzyme-labelled antibody adapted for use in a homogeneous immunoassay is provided. The enzyme-labelled antibody is a conjugate of an enzyme with two or more different monoclonal antibodies, each of the monoclonal antibodies being capable of specifically recognizing and binding to a different epitope of the same antigen. By using the enzyme-labelled antibody in the homogeneous enzyme immunoassay process, an analyte can be quantitatively analyzed at a higher sensitivity through a simple operation. Also provided is a dry immunoassay element comprising an immunological reaction layer containing the enzyme-labelled antibody. By the provision of such an immunoassay element, a further simplified quick analysis of an analyte is realized to give an accurate result.

摘要翻译： 提供适用于均相免疫测定法的酶标记抗体。 酶标记的抗体是酶与两种或更多种不同单克隆抗体的缀合物，每种单克隆抗体能够特异性识别并结合相同抗原的不同表位。 通过在均相酶免疫测定法中使用酶标记的抗体，可以通过简单的操作以更高的灵敏度对分析物进行定量分析。 还提供了包含含有酶标记抗体的免疫反应层的干免疫测定元件。 通过提供这样的免疫测定元件，实现了分析物的进一步简化的快速分析以给出准确的结果。