公开(公告)号：US20080311243A1

公开(公告)日：2008-12-18

申请号：US12138919

申请日：2008-06-13

申请人： Thavaratnam Vasanthan ,  Feral Temelli ,  Beljit Ghotra ,  Mohamed Khan

发明人： Thavaratnam Vasanthan ,  Feral Temelli ,  Beljit Ghotra ,  Mohamed Khan

IPC分类号： C12C1/073

CPC分类号： C08B37/0003 ,  C08B37/0024 ,  C08L3/02 ,  C08L5/04 ,  C12P19/04 ,  C12P19/14

摘要： The invention describes improved methods of preparing high concentration and high viscosity beta-glucan concentrates. More specifically, the invention describes methods wherein beta-glucan is concentrated from bran, whole grain and endosperm flours through various slurrying steps in a high concentration alcohol media utilizing various combinations of enzyme and alkali treatment steps.

摘要翻译： 本发明描述了制备高浓度和高粘度β-葡聚糖浓缩物的改进方法。 更具体地，本发明描述了利用酶和碱处理步骤的各种组合，通过各种浆料化步骤在高浓度醇介质中，从麸，全谷粒和胚乳面粉中浓缩β-葡聚糖的方法。

公开(公告)号：US20080248508A1

公开(公告)日：2008-10-09

申请号：US11985057

申请日：2007-11-13

申请人： Shenda Baker ,  William P. Wiesmann

发明人： Shenda Baker ,  William P. Wiesmann

IPC分类号： G01N33/53 ,  C08B37/08

CPC分类号： C08B37/003 ,  C08B37/0003 ,  G01N33/5308 ,  G01N2333/525 ,  G01N2333/5412 ,  G01N2333/545 ,  G01N2400/28

摘要： Chitosan is a natural product having wide range of applications in the food and cosmetic industries. Food and commodity grade chitosan are laden with pyrogens, such as endotoxins and proteins which limit its applicability in the biological and medical arenas, as minute amounts of endotoxins may induce adaptive and innate responses when contacted with mammalian tissue, pharmaceuticals and biomedical devices. Due to chitosan's ability to avidly bind endotoxin and other pyrogens, they are difficult to remove. The present invention is directed to methods for purifying chitosan from shells, food and commodity grade chitosan into ultra-pure, low endotoxin chitosan having biological and medical applicability. Additionally, the present invention is also directed to a method of determining the pyrogenicity of the ultra-pure low endotoxin chitosan.

摘要翻译： 壳聚糖是一种在食品和化妆品行业广泛应用的天然产品。 食品和商品级脱乙酰壳多糖含有热原，例如限制其在生物和医学领域中的适用性的内毒素和蛋白质，因为当与哺乳动物组织，药物和生物医学装置接触时，微量的内毒素可诱导适应性和先天反应。 由于壳聚糖能够强烈结合内毒素和其他热原，因此难以去除。 本发明涉及从壳，食品级和商品级壳聚糖中提取壳聚糖成为具有生物和医学适用性的超纯低密度内毒素壳聚糖的方法。 此外，本发明还涉及确定超纯低毒素壳聚糖的致热原性的方法。

公开(公告)号：US20080138865A1

公开(公告)日：2008-06-12

申请号：US11774037

申请日：2007-07-06

申请人： Vidhya RANGASWAMY ,  Santosh Vyas ,  Dharmendra Jain ,  Vedapuri Nataraj ,  Harshad Velankar ,  Arnab Kapat ,  Sonti Venkata Rama Krishna

发明人： Vidhya RANGASWAMY ,  Santosh Vyas ,  Dharmendra Jain ,  Vedapuri Nataraj ,  Harshad Velankar ,  Arnab Kapat ,  Sonti Venkata Rama Krishna

IPC分类号： C12P19/04

CPC分类号： C12P19/26 ,  C08B37/0003 ,  C08B37/0072

摘要： The present invention provides an efficient and improved process for the production and purification of high molecular weight hyaluronic acid (HA). One embodiment relates to providing growth conditions for production of high molecular weight HA in Streptococcus zooepidemicus. For example, growth in medium comprising a lower protein concentration, e.g., 1% casein hydrolyzate, and a higher sugar concentration, e.g., 5% sucrose, gave 5-6 g/L of high molecular weight HA in the range of 3.5-4.0×106 Da. The present invention also provides an efficient process for the purification of HA comprising treatment with silica gel then active carbon, and subsequent diafiltration with less volume of solvent.

摘要翻译： 本发明提供高效和改进的高分子量透明质酸（HA）的生产和纯化方法。 一个实施方案涉及提供用于生产链球菌动物疫苗的高分子量HA的生长条件。 例如，含有较低蛋白质浓度的培养基（例如1％酪蛋白水解产物）和较高糖浓度（例如5％蔗糖）的生长得到5-6g / L 3.5-4.0范围内的高分子量HA x10 6 Da。 本发明还提供了用于纯化HA的有效方法，包括用硅胶然后用活性炭处理，随后用较少体积的溶剂进行渗滤。

公开(公告)号：US20070141678A1

公开(公告)日：2007-06-21

申请号：US11604453

申请日：2006-11-27

申请人： Phillip Richard Green ,  Karunakaran Narasimhan

发明人： Phillip Richard Green ,  Karunakaran Narasimhan

IPC分类号： C12P19/28 ,  C08B37/00

CPC分类号： C08B37/0003 ,  C07H1/08

摘要： Processes for purifying oligosaccharides including contacting a basic oligosaccharide solution with a basic boronate resin to bind monosaccharides to the basic boronate resin and obtain a purified oligosaccharide solution. The basic boronate resin can bind about 50% of the monosaccharides and about 10% of oligosaccharides in the basic oligosaccharide solution, by weight of total carbohydrates therein.

摘要翻译： 用于纯化寡糖的方法，包括使碱性寡糖溶液与碱式硼酸酯树脂接触以使单糖与碱式硼酸酯树脂结合，并获得纯化的寡糖溶液。 基本的硼酸酯树脂可以结合约50％的单糖和约10％的寡糖在碱性寡糖溶液中，其中总碳水化合物的重量。

公开(公告)号：US20060228381A1

公开(公告)日：2006-10-12

申请号：US11396108

申请日：2006-03-31

申请人： Brian Bahler ,  Tsu-shun Lee ,  Jason Lotvin ,  Mark Ruppen ,  Pamela Charbonneau

发明人： Brian Bahler ,  Tsu-shun Lee ,  Jason Lotvin ,  Mark Ruppen ,  Pamela Charbonneau

IPC分类号： A61K39/09 ,  C08B37/00 ,  C12P19/04 ,  C12N1/21

CPC分类号： A61K39/085 ,  C08B37/0003 ,  C12N1/06 ,  C12N1/20 ,  C12P1/04 ,  C12P19/04

摘要： A process for reducing the protein content and preserving the capsular polysaccharide content in a complex cellular Streptococcus pneumoniae lysate broth prior to purification is described. Utilizing pH reduction after cellular lysis has resulted in a purified polysaccharide that consistently meets the protein specification, and higher recovery yields of polysaccharide during the purification process.

摘要翻译： 描述了在纯化之前降低蛋白质含量并保持复合细胞肺炎链球菌裂解物肉汤中的荚膜多糖含量的方法。 在细胞裂解后利用pH降低导致纯化多糖，其一致地符合蛋白质规格，并且在纯化过程中产生更高的多糖回收率。

公开(公告)号：US20060122149A1

公开(公告)日：2006-06-08

申请号：US10554288

申请日：2004-04-30

申请人： Mark Redmond ,  David Fielder

发明人： Mark Redmond ,  David Fielder

IPC分类号： A61K31/715 ,  C12P19/04

CPC分类号： A61K36/8998 ,  A61K31/716 ,  A61K36/899 ,  C08B37/0003 ,  C08B37/0024 ,  C08L5/00 ,  A61K2300/00

摘要： A novel method to extract and purify cereal β-glucan is described. Cereal β glucans are distinctive polymers of glucose differentiated from other polymers not only by their source but also by their physicochemical properties. The high purity of the cereal β-glucan obtained according to the present invention allows for the preparation of clear, colourless viscous liquid preparations. These liquid preparations are stable to gelling effects when kept at ambient temperatures and low ash concentrations. Compositions comprising β (1-3) β (1-4) glucan and a freezing point depressant are also, described.

摘要翻译： 描述了一种提取和纯化谷物β-葡聚糖的新方法。 谷类β葡聚糖是不同于其他聚合物的葡萄糖的独特聚合物，不仅通过其来源，而且还通过其物理化学性质。 根据本发明获得的谷类β-葡聚糖的高纯度允许制备澄清的无色粘稠液体制剂。 当保持在环境温度和低灰分浓度时，这些液体制剂对胶凝效果是稳定的。 还描述了包含β（1-3）β（1-4）葡聚糖和冰点抑制剂的组合物。

公开(公告)号：US07022838B2

公开(公告)日：2006-04-04

申请号：US10945482

申请日：2004-09-20

申请人： James M. Gaddy ,  Penelope A. Patton

发明人： James M. Gaddy ,  Penelope A. Patton

IPC分类号： C08B37/00 ,  C07H1/00 ,  C07H1/04 ,  C07H1/06

CPC分类号： C08B37/0003 ,  C08B37/0033

摘要： Processes are disclosed for the purification and recovery of polysaccharide gums from an aqueous solution, particularly xanthan gum from a fermentation broth. An aqueous solution of at least one polysaccharide gum is mixed with a non-solvent stream comprising water and a subprecipitant level of a non-solvent of the polysaccharide gum. The mixture is concentrated to increase the polysaccharide gum concentration, and optionally undergoes a heat treatment. Additional non-solvent is added to the concentrated mixture to precipitate the polysaccharide gum. The precipitated gum is dried after being separated from the liquid component of the mixture. The removed liquid component can be recycled to the earlier step in the process in which the polysaccharide gum solution is mixed with the non-solvent stream.

公开(公告)号：US20050271613A1

公开(公告)日：2005-12-08

申请号：US11090052

申请日：2005-03-28

申请人： Toshio Suzuki ,  Seiji Nakamura ,  Satoshi Nakayama ,  Kouji Nishikawa

发明人： Toshio Suzuki ,  Seiji Nakamura ,  Satoshi Nakayama ,  Kouji Nishikawa

IPC分类号： A61K31/715 ,  A61K7/06 ,  A61K7/11

CPC分类号： C08B37/0024 ,  A23L29/271 ,  A23L33/21 ,  A61K8/73 ,  A61K31/716 ,  A61Q19/00 ,  C08B37/0003 ,  C08L5/00 ,  C12P19/04 ,  C12R1/645 ,  Y02A50/463

摘要： An aqueous β-1,3-1,6-D-glucan solution, wherein 1HNMR spectra on its solution containing in 1N aqueous sodium hydroxide heavy solution have two signals of 4.7 ppm and 4.5 ppm and the viscosity (BM type rotary-viscometer, 12 rpm) of its solution at 30° C., pH5.0, and its concentration 0.5% (w/v) is 50 cP ([mPa.s]) or less.

摘要翻译： 其中在含有1N氢氧化钠水溶液的溶液中的1 HNMR光谱的β-1,3-1,6-D-葡聚糖水溶液具有4.7ppm和4.5ppm的两个信号，并且 其溶液在30℃，pH5.0，其浓度为0.5％（w / v）下的粘度（BM型旋转粘度计，12rpm）为50cP（[mPa.s]）以下。

公开(公告)号：US06936173B2

公开(公告)日：2005-08-30

申请号：US10104609

申请日：2002-03-22

申请人： Shawn DeFrees

发明人： Shawn DeFrees

IPC分类号： C07H1/06 ,  B01D61/02 ,  B01D61/04 ,  B01D61/16 ,  B01D61/24 ,  B01D71/56 ,  C02F1/44 ,  C08B30/00 ,  C08B37/00 ,  C13B10/08 ,  C13B20/12 ,  C13B20/16 ,  C13B30/08 ,  C13B35/08 ,  C13K13/00 ,  B01D61/00

CPC分类号： C13B20/165 ,  B01D61/022 ,  B01D61/025 ,  B01D61/027 ,  B01D61/04 ,  B01D2311/04 ,  C08B37/0003 ,  C13K13/00 ,  Y02P20/582 ,  B01D2311/18 ,  B01D2311/12

摘要： The invention provides methods for purifying carbohydrates, including oligosaccharides, nucleotide sugars, and related compounds, by use of ultrafiltration, nanofiltration and/or reverse osmosis. The carbohydrates are purified away from undesired contaminants such as compounds present in reaction mixtures following enzymatic synthesis or degradation of oligosaccharides.

摘要翻译： 本发明提供了通过使用超滤，纳滤和/或反渗透来纯化碳水化合物的方法，包括寡糖，核苷酸糖和相关化合物。 将碳水化合物从不期望的污染物纯化出来，例如低聚糖的酶合成或降解后存在于反应混合物中的化合物。

公开(公告)号：US20050038241A1

公开(公告)日：2005-02-17

申请号：US10945482

申请日：2004-09-20

申请人： James Gaddy ,  Penelope Patton

发明人： James Gaddy ,  Penelope Patton

IPC分类号： C08B37/00 ,  C08B37/04 ,  C08B37/06 ,  C08B35/00

CPC分类号： C08B37/0003 ,  C08B37/0033

摘要： Processes are disclosed for the purification and recovery of polysaccharide gums from an aqueous solution, particularly xanthan gum from a fermentation broth. An aqueous solution of at least one polysaccharide gum is mixed with a non-solvent stream comprising water and a subprecipitant level of a non-solvent of the polysaccharide gum. The mixture is concentrated to increase the polysaccharide gum concentration, and optionally undergoes a heat treatment. Additional non-solvent is added to the concentrated mixture to precipitate the polysaccharide gum. The precipitated gum is dried after being separated from the liquid component of the mixture. The removed liquid component can be recycled to the earlier step in the process in which the polysaccharide gum solution is mixed with the non-solvent stream.