公开(公告)号：US08830476B2

公开(公告)日：2014-09-09

申请号：US13423825

申请日：2012-03-19

Applicant: Matthew J. Berg ,  Gorden Videen

Inventor： Matthew J. Berg ,  Gorden Videen

IPC: G01B9/021 ,  G01N15/02

CPC classification number: G01N15/0227 ,  G01N15/1434 ,  G01N15/1463 ,  G01N15/1475 ,  G01N2015/0096 ,  G01N2015/1445

Abstract: Methods and apparatuses provide holographic contact-free imaging of aerosol particles in an efficient manner. One apparatus for holographic imaging of an aerosol particle may include: a delivery device configured to deliver the particle into a region; a light source for outputting a first beam of light and a second beam of light, wherein the first beam travels into the region producing a first light wave which is un-scattered by the particle and a second light wave that is scattered by the particle, and the second beam does not travel into the region; a beam splitter for combining the second beam with the scattered light of the first beam into combined interference light; an image sensor for sensing an interference pattern created by the combined interference light; and an image processor configured to generate an image of the aerosol particle based on the sensed interference pattern.

Abstract translation: 方法和装置以有效的方式提供气溶胶颗粒的全息无接触成像。 用于气溶胶颗粒的全息成像的一种装置可以包括：配置成将颗粒输送到区域中的输送装置; 用于输出第一光束和第二光束的光源，其中所述第一光束进入所述区域，所述区域产生未被所述粒子散射的第一光波和由所述粒子散射的第二光波， 并且第二光束不会进入该区域; 分束器，用于将第二光束与第一光束的散射光组合成组合的干涉光; 用于感测由所述组合干涉光产生的干涉图案的图像传感器; 以及图像处理器，被配置为基于所感测的干涉图案来生成气溶胶粒子的图像。

公开(公告)号：US20140248713A1

公开(公告)日：2014-09-04

申请号：US14193714

申请日：2014-02-28

Applicant: Commissariat à l'énergie atomique et aux énergies alternatives

Inventor： Cédric ALLIER ,  Fabrice NAVARRO Y GARCIA ,  Vincent POHER ,  Patrick POUTEAU

IPC: G01N21/64

CPC classification number: G01N21/6428 ,  G01N15/1475 ,  G01N21/4788 ,  G01N21/6456 ,  G01N2015/1454 ,  G02B27/42 ,  G03H1/0443 ,  G03H1/0866 ,  G03H2001/0447

Abstract: This method for observing at least one object is applied by means of an imaging system. It includes the following steps: illuminating the object(s), with a light source, acquiring, by means of a photodetector array, a diffraction pattern, the diffraction pattern corresponding to an image of the waves diffracted by the object(s) when they are illuminated along an illumination direction, the object(s) such as biological entities, being positioned between the light source and the photodetector array along the illumination direction. The method further comprises, prior to the illumination step, addition of at least one marker in contact with the object(s), said or each marker being adapted to bind onto a corresponding object, the binding of said marker onto said object being adapted for increasing at least one characteristic quantity from among the absorption and the optical phase shift of said object.

Abstract translation: 用于观察至少一个物体的该方法通过成像系统来应用。 它包括以下步骤：用光源照射物体，通过光电检测器阵列获取衍射图案，衍射图案对应于由物体衍射的波的图像，当它们 沿着照明方向被照射，诸如生物体的物体沿着照明方向位于光源和光电检测器阵列之间。 该方法还包括在照明步骤之前，添加与物体接触的至少一个标记，所述或每个标记适于结合到相应的物体上，所述标记与所述物体的结合适于 从所述物体的吸收和光学相移中增加至少一个特征量。

公开(公告)号：US20140233826A1

公开(公告)日：2014-08-21

申请号：US14347912

申请日：2012-09-26

Applicant: Sos S. Agaian ,  Clara M. Mosquera Lopez ,  Ali Almuntashri ,  Richard Metzler

Inventor： Sos S. Agaian ,  Clara M. Mosquera Lopez ,  Ali Almuntashri ,  Richard Metzler

IPC: G06T7/00 ,  G06K9/00

CPC classification number: G06K9/0014 ,  A61B5/4381 ,  A61B5/7203 ,  A61B5/725 ,  A61B5/7257 ,  A61B5/726 ,  A61B5/7267 ,  A61B10/0241 ,  G01N15/1475 ,  G06F19/321 ,  G06K9/00127 ,  G06K9/00147 ,  G06K9/46 ,  G06K9/6228 ,  G06T5/002 ,  G06T5/20 ,  G06T7/0012 ,  G06T7/13 ,  G06T7/181 ,  G06T7/90 ,  G06T2207/10024 ,  G06T2207/10056 ,  G06T2207/20036 ,  G06T2207/20081 ,  G06T2207/30024 ,  G06T2207/30081 ,  G06T2207/30096 ,  G16H30/20 ,  G16H50/20 ,  G16H50/30 ,  G16H50/70

Abstract: The invention provides systems and methods for detection, grading, scoring and tele-screening of cancerous lesions. A complete scheme for automated quantitative analysis and assessment of human and animal tissue images of several types of cancers is presented. Various aspects of the invention are directed to the detection, grading, prediction and staging of prostate cancer on serial sections/slides of prostate core images, or biopsy images. Accordingly, the invention includes a variety of sub-systems, which could be used separately or in conjunction to automatically grade cancerous regions. Each system utilizes a different approach with a different feature set. For instance, in the quantitative analysis, textural-based and morphology-based features may be extracted at image- and (or) object-levels from regions of interest. Additionally, the invention provides sub-systems and methods for accurate detection and mapping of disease in whole slide digitized images by extracting new features through integration of one or more of the above-mentioned classification systems. The invention also addresses the modeling, qualitative analysis and assessment of 3-D histopathology images which assist pathologists in visualization, evaluation and diagnosis of diseased tissue. Moreover, the invention includes systems and methods for the development of a tele-screening system in which the proposed computer-aided diagnosis (CAD) systems. In some embodiments, novel methods for image analysis (including edge detection, color mapping characterization and others) are provided for use prior to feature extraction in the proposed CAD systems.

Abstract translation: 本发明提供用于癌性病变的检测，分级，评分和远程筛查的系统和方法。 提出了一种用于自动定量分析和评估几种类型癌症的人和动物组织图像的完整方案。 本发明的各个方面涉及前列腺核心图像或活检图像的连续切片/载玻片上的前列腺癌的检测，分级，预测和分期。 因此，本发明包括各种子系统，其可以单独使用或结合使用以自动分级癌症区域。 每个系统使用不同的方法与不同的功能集。 例如，在定量分析中，可以从感兴趣区域的图像和（或）对象层面提取基于纹理和基于形态的特征。 此外，本发明提供了通过一个或多个上述分类系统的集成来提取新特征来准确地检测和映射整个幻灯片数字化图像中的疾病的子系统和方法。 本发明还涉及三维组织病理学图像的建模，定性分析和评估，其帮助病理学家在患病组织的可视化，评估和诊断中。 此外，本发明包括用于开发远程筛选系统的系统和方法，其中所提出的计算机辅助诊断（CAD）系统。 在一些实施例中，提供用于图像分析（包括边缘检测，颜色映射表征等）的新方法，用于在所提出的CAD系统中的特征提取之前使用。

公开(公告)号：US08780181B2

公开(公告)日：2014-07-15

申请号：US13132713

申请日：2009-12-04

Applicant: Tom Olesen ,  Martin Christian Valvik ,  Niels Agersnap Larsen ,  Rasmus Helmsby Sandberg

Inventor： Tom Olesen ,  Martin Christian Valvik ,  Niels Agersnap Larsen ,  Rasmus Helmsby Sandberg

IPC: H04N13/02 ,  G01N15/14 ,  H04N13/00 ,  G01N17/00

CPC classification number: G02B21/367 ,  G01N15/1434 ,  G01N15/1475 ,  G01N17/00 ,  G01N2015/1445 ,  G01N2015/1452 ,  G02B21/002 ,  G06K9/00134 ,  G06T7/0012 ,  G06T2207/10016 ,  G06T2207/10061 ,  G06T2207/30024 ,  G06T2207/30101 ,  H04N13/189 ,  H04N13/239 ,  H04N13/296

Abstract: The invention relates to an apparatus, a method and a system for obtaining a plurality of images of a sample arranged in relation to a sample device. The apparatus comprises at least a first optical detection assembly having an optical axis and at least one translation unit arranged to move the sample device and the first optical detection assembly relative to each other. The movement of the sample device and the first optical detection assembly relative to each other is along a scanning path, which defines an angle theta relative to the optical axis, wherein theta is larger than zero.

Abstract translation: 本发明涉及一种用于获得相对于样本装置布置的样本的多个图像的装置，方法和系统。 该装置至少包括具有光轴的第一光学检测组件和布置成使样本装置和第一光学检测组件相对于彼此移动的至少一个平移单元。 样品装置和第一光学检测组件相对于彼此的运动沿着扫描路径，该扫描路径限定相对于光轴的角度θ，其中θ大于零。

公开(公告)号：US08759080B2

公开(公告)日：2014-06-24

申请号：US12364152

申请日：2009-02-02

Applicant: Josef A. Graessle ,  Stephen B. Schenk ,  Michael C. Lea

Inventor： Josef A. Graessle ,  Stephen B. Schenk ,  Michael C. Lea

IPC: C12M1/34 ,  G06K9/00

CPC classification number: G01N15/1475

Abstract: A biological growth plate scanner includes a multi-color illumination system that illuminates a biological growth plate with different illumination colors. A monochromatic image capture device captures images of the biological growth plate during illumination of the growth plate with each of the illumination colors. A processor combines the images to form a composite multi-color image, and/or individual components of the composite image, and analyzes the composite image to produce an analytical result such as a colony count or a presence/absence result. The biological growth plate scanner may include both front and back illumination components. The back illumination component may include a diffuser element disposed under the biological growth plate. The diffuser element receives light from one or more laterally disposed illumination sources, and distributes the light to illuminate a back side of the biological growth plate. The illumination sources in the front and back illumination components may take the form of sets of light emitting diodes (LEDs) that can be independently controlled by the processor.

Abstract translation: 生物生长板扫描器包括用不同照明颜色照射生物生长板的多色照明系统。 单色图像捕获装置在以每种照明颜色照射生长板的过程中捕获生物生长板的图像。 处理器组合图像以形成复合多色图像和/或合成图像的各个组件，并分析合成图像以产生分析结果，例如菌落计数或存在/不存在结果。 生物生长板扫描器可以包括前照射组件和后照射组件。 后照明部件可以包括设置在生物生长板下方的漫射元件。 扩散器元件接收来自一个或多个横向设置的照明源的光，并且分配光以照射生物生长板的背面。 前后照明部件中的照明源可以采取可由处理器独立控制的一组发光二极管（LED）的形式。

公开(公告)号：US20140071452A1

公开(公告)日：2014-03-13

申请号：US14023455

申请日：2013-09-10

Applicant: Jason W. Fleischer

Inventor： Jason W. Fleischer

IPC: G01N21/59

CPC classification number: G01N21/59 ,  B01L3/502715 ,  G01N15/1434 ,  G01N15/147 ,  G01N15/1475 ,  G01N2015/1006 ,  G01N2015/1445 ,  G02B21/086 ,  G02B21/367

Abstract: A microscope is disclosed, the microscope having a light source defining an optical axis along a Z direction and a detector disposed in X-Y direction, orthogonal to the optical axis, the detector configured to capture images of an object. The microscope includes a fluid channel having an inlet and an outlet configured with a fluid flow to transport the object from the inlet to the outlet. The detector is configured to capture a plurality of images of the object as the object moves from the inlet to the outlet. The plurality of images of the object may have different heights of the sample with respect to the detector as the sample flows through the channel. The channel may be tilted with respect to the optical axis. The detector may be tilted with respect to the optical axis.

Abstract translation: 公开了一种显微镜，显微镜具有限定沿Z方向的光轴的光源和与光轴正交的X-Y方向的检测器，检测器被配置为捕获物体的图像。 显微镜包括具有入口和出口的流体通道，该入口和出口配置有流体流，以将物体从入口输送到出口。 检测器被配置为当对象从入口移动到出口时捕获对象的多个图像。 当样本流过通道时，对象的多个图像可以具有相对于检测器的样本的不同高度。 通道可以相对于光轴倾斜。 检测器可以相对于光轴倾斜。

公开(公告)号：US08639012B2

公开(公告)日：2014-01-28

申请号：US12726628

申请日：2010-03-18

Applicant: Xin Heng ,  Paul Patt

Inventor： Xin Heng ,  Paul Patt

IPC: G06K9/00

CPC classification number: G01N21/6486 ,  G01J3/02 ,  G01J3/0208 ,  G01J3/04 ,  G01J3/10 ,  G01J3/36 ,  G01J3/4406 ,  G01N15/1425 ,  G01N15/1429 ,  G01N15/147 ,  G01N15/1475 ,  G01N21/6458 ,  G01N2021/6419 ,  G01N2021/6421

Abstract: A system for performing high-speed, high-resolution imaging cytometry utilizes a line-scan sensor. A cell to be characterized is transported past a scan region. An optical system focuses an image of a portion of the scan region onto at least one linear light sensor, and repeated readings of light falling on the sensor are taken while a cell is transported though the scan region. The system may image cells directly, or may excite fluorescence in the cells and image the resulting light emitted from the cell by fluorescence. The system may provide a narrow band of illumination at the scan region. The system may include various filters and imaging optics that enable simultaneous multicolor fluorescence imaging cytometry. Multiple linear sensors may be provided, and images gathered by the individual sensors may be combined to construct an image having improved signal-to-noise characteristics.

Abstract translation: 用于执行高速，高分辨率成像细胞计数的系统利用线扫描传感器。 待特征化的细胞被运送通过扫描区域。 光学系统将扫描区域的一部分的图像聚焦到至少一个线性光传感器上，并且在单元格被传送通过扫描区域的同时拍摄落在传感器上的光的重复读数。 该系统可以直接对细胞进行成像，或者可以激发细胞中的荧光，并通过荧光对从细胞发出的所得光进行成像。 该系统可以在扫描区域提供窄带照明。 该系统可以包括能够同时进行多色荧光成像细胞计数的各种滤光器和成像光学元件。 可以提供多个线性传感器，并且可以组合由各个传感器收集的图像以构建具有改进的信噪比特性的图像。

公开(公告)号：US08632025B2

公开(公告)日：2014-01-21

申请号：US12992413

申请日：2009-05-14

Applicant: Dario Pierri ,  Martin Heine ,  Urs Dübendorfer

Inventor： Dario Pierri ,  Martin Heine ,  Urs Dübendorfer

IPC: B02C1/10

CPC classification number: B02C25/00 ,  B02C4/28 ,  B02C4/32 ,  G01N15/147 ,  G01N15/1475 ,  G01N21/85 ,  G01N2015/0019 ,  G01N2015/1472 ,  G01N2021/154 ,  G01N2021/1776 ,  G01N2021/8592

Abstract: A stream of ground particles is characterized by irradiating it with electromagnetic radiation and detecting electromagnetic radiation emitted from the irradiated particles. The detecting system includes an imaging system and a color image sensor for imaging the particles by way of the electromagnetic radiation emitted by the particles. The color image sensor has sensor image elements for spectrally selective detection of the electromagnetic radiation.

Abstract translation: 研磨颗粒流的特征在于用电磁辐射照射并检测从辐照颗粒发射的电磁辐射。 检测系统包括成像系统和彩色图像传感器，用于通过由颗粒发射的电磁辐射对颗粒进行成像。 彩色图像传感器具有用于光谱选择性检测电磁辐射的传感器图像元件。

公开(公告)号：US08625920B2

公开(公告)日：2014-01-07

申请号：US10872975

申请日：2004-06-21

Applicant: James W. Bacus ,  James V. Bacus

Inventor： James W. Bacus ,  James V. Bacus

IPC: G06K9/40

CPC classification number: G06T3/4038 ,  G01N15/1475 ,  G02B21/367 ,  G06F19/00 ,  G06F19/321 ,  G06F19/3418 ,  G06K9/00127 ,  G16H10/40 ,  G16H40/63 ,  Y10S707/99942 ,  Y10S707/99943 ,  Y10S707/99945

Abstract: The invention is directed to a method and apparatus for constructing a virtual microscope slide. The method encompasses the construction of a data structure by digitally scanning a specimen and acquiring digital image data of a series of digitized adjacent image segments of the specimen. The image segments are useable to provide an overall magnified image of the specimen as well as higher magnification images of selected points on the specimen. The scanned digital image data can be stored and transferred over an Internet or intranet communication channel. A control program is provided for the data structure for viewing the overall magnified image and higher magnification images of selected points on the specimen. The control program allows for the selection of a point on the overall magnified image, and display from the stored digital image data a corresponding higher magnification image of the selected point.

Abstract translation: 本发明涉及一种用于构建虚拟显微镜幻灯片的方法和装置。 该方法包括通过数字扫描样本并获取样本的一系列数字化相邻图像片段的数字图像数据来构建数据结构。 图像段可用于提供样本的整体放大图像以及样品上所选点的较高放大图像。 扫描的数字图像数据可以通过因特网或内联网通信信道进行存储和传送。 提供了一种控制程序，用于数据结构，用于查看样本上所选点的整体放大图像和较高放大倍数的图像。 控制程序允许选择整个放大图像上的点，并且从所存储的数字图像数据中显示所选点的相应较高放大倍数的图像。

公开(公告)号：US20140004554A1

公开(公告)日：2014-01-02

申请号：US13925295

申请日：2013-06-24

Applicant: Abbott Point of Care, Inc.

Inventor： Graham Davis ,  Stephen C. Wardlaw ,  Robert A. Levine ,  Darryn W. Unfricht ,  Niten Lalpuria ,  Paul G. Mitsis

IPC: G01N33/72

CPC classification number: G01N33/726 ,  G01N15/1475 ,  G01N2015/0073

Abstract: A method and apparatus for determining at least one hemoglobin related parameter of a whole blood sample is provided. The method includes the steps of: a) depositing the sample into an analysis chamber adapted to quiescently hold the sample for analysis, the chamber defined by an interior surface of a first panel, and an interior surface of a second panel, and the chamber has a height extending between the interior surfaces of the panels, wherein the chamber is configured to increase the oxygenation state of the sample to a substantially oxygenated state within a predetermined amount of time after entry into the chamber; b) imaging the at least one red blood cell contacting the interior surfaces, and producing image signals; c) determining an optical density of at least a portion of the imaged red blood cell contacting both interior surfaces; and d) determining the at least one hemoglobin related parameter of the red blood cell contacting the interior surfaces, using the determined optical density and a molar extinction coefficient for oxygenated hemoglobin.

Abstract translation: 提供了一种用于确定全血样品的至少一种血红蛋白相关参数的方法和装置。 该方法包括以下步骤：a）将样品沉积到适于静态保持样品进行分析的分析室中，所述室由第一面板的内表面和第二面板的内表面限定，并且所述室具有 在所述面板的内表面之间延伸的高度，其中所述腔被配置为在进入所述腔室之后的预定量的时间内将所述样品的氧合状态增加到基本上氧合状态; b）对与内表面接触的至少一个红细胞进行成像，并产生图像信号; c）确定接触两个内表面的成像红细胞的至少一部分的光密度; 以及d）使用确定的光密度和氧合血红蛋白的摩尔消光系数来确定与内表面接触的红细胞的至少一种血红蛋白相关参数。