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    Method for producing a polynucleotide library in vitro by mismatch repair of heteroduplexes
    83.
    发明授权
    Method for producing a polynucleotide library in vitro by mismatch repair of heteroduplexes 失效
    通过异源双链体的错配修复在体外制备多核苷酸文库的方法

    公开(公告)号：US06783941B2

    公开(公告)日：2004-08-31

    申请号：US10004993

    申请日：2001-12-05

    申请人： Jesper Vind

    发明人： Jesper Vind

    IPC分类号： C12Q168

    CPC分类号： C12N15/1027

    摘要： A method for making polynucleotide libraries that comprise new recombined polynucleotides by using mismatch repair enzymes or proteins to shuffle at least two variants of the same polynucleotide or at least two homologous polynucleotides through a Recombinatorial Chain Reaction, RCR.

    摘要翻译： 通过使用错配修复酶或蛋白质来制备包含新的重组多核苷酸的多核苷酸文库的方法，其通过重组体链式反应RCR混洗相同多核苷酸的至少两种变体或至少两种同源多核苷酸。

    Expression Cloning Method Suitable for Selecting Library Clones Producing a Polypeptide of Interest
    84.
    发明申请
    Expression Cloning Method Suitable for Selecting Library Clones Producing a Polypeptide of Interest 审中-公开
    表达克隆方法适用于选择产生多肽兴趣的文库克隆

    公开(公告)号：US20100221783A1

    公开(公告)日：2010-09-02

    申请号：US12599384

    申请日：2008-05-07

    申请人： Jesper Vind

    发明人： Jesper Vind

    IPC分类号： C12P21/04

    CPC分类号： C12N15/80 ,  C12P21/02

    摘要： The present invention relates to methods for producing a recombinant polypeptide of interest, the method comprising the steps of: a) providing a polynucleotide library encoding one or more polypeptides of interest, wherein the library was prepared in an expression cloning vector comprising at least the following elements: i) a polynucleotide encoding a selectable marker; ii) a fungal replication initiation sequence, preferably an autonomously replicating sequence (ARS); and iii) a polynucleotide comprising in sequential order: a promoter derived from a fungal cell, a cloning-site into which the library is cloned, and a transcription terminator; b) transforming a mutant of a parent filamentous fungal host cell with the library, wherein the frequency of non-homologous recombination in the mutant has been decreased compared to the parent; c) culturing the transformed host cell obtained in (b) under conditions suitable for expression of the polynucleotide library; and d) selecting a transformed host cell which produces the polypeptide of interest.

    摘要翻译： 本发明涉及用于产生感兴趣的重组多肽的方法，所述方法包括以下步骤：a）提供编码一种或多种感兴趣多肽的多核苷酸文库，其中所述文库在包含至少以下的表达克隆载体中制备 元素：i）编码可选择标记的多核苷酸; ii）真菌复制起始序列，优选自主复制序列（ARS）; 和iii）包含序列顺序的多核苷酸：源自真菌细胞的启动子，克隆文库的克隆位点和转录终止子; b）用文库转化亲本丝状真菌宿主细胞的突变体，其中与亲本相比，突变体中非同源重组的频率已经降低; c）在适于表达多核苷酸文库的条件下培养（b）中获得的转化的宿主细胞; 和d）选择产生感兴趣多肽的转化的宿主细胞。

    Expression cloning methods in filamentous fungi
    87.
    发明申请
    Expression cloning methods in filamentous fungi 审中-公开
    丝状真菌中的表达克隆方法

    公开(公告)号：US20050170350A1

    公开(公告)日：2005-08-04

    申请号：US10504646

    申请日：2003-02-18

    申请人： Mary Stringer ,  Kirk Schnorr ,  Jesper Vind

    发明人： Mary Stringer ,  Kirk Schnorr ,  Jesper Vind

    IPC分类号： C12N15/65 ,  C12N15/80 ,  C12Q1/68 ,  C12N1/16 ,  C12N15/74 ,  G01N33/53

    CPC分类号： C12N15/65 ,  C12N15/80

    摘要： Methods for screening a polynucleotide library for a polypeptide with a property of interest in a filamentous fungal host cell, in a manner which allows quick and easy subsequent characterization of the polypeptide, using an expression cloning vector comprising at least a polynucleotide encoding a selectable marker in which the translation initiation start site of the marker-encoding sequence comprises a crippled consensus Kozak sequence, a fungal replication initiation sequence, and a promoter with a cloning-site into which the library is cloned, and a transcription terminator.

    摘要翻译： 使用包含至少编码可选择标记的多核苷酸的表达克隆载体以允许快速且容易地随后表征多肽的方式筛选丝状真菌宿主细胞中具有感兴趣性质的多肽的多核苷酸文库的方法 其标记编码序列的翻译起始起始位点包含残基共有的Kozak序列，真菌复制起始序列和具有克隆文库的克隆位点的启动子和转录终止子。

    Process and composition for desizing cellulosic fabric with an enzyme hybrid
    88.
    发明授权
    Process and composition for desizing cellulosic fabric with an enzyme hybrid 失效
    用酶杂交脱浆纤维素织物的方法和组合物

    公开(公告)号：US6017751A

    公开(公告)日：2000-01-25

    申请号：US812829

    申请日：1997-03-06

    申请人： Claus von der Osten ,  Mads E. Bjornvad ,  Jesper Vind ,  Michael Dolberg Rasmussen

    发明人： Claus von der Osten ,  Mads E. Bjornvad ,  Jesper Vind ,  Michael Dolberg Rasmussen

    IPC分类号： C11D3/386 ,  C12N9/20 ,  C12N9/24 ,  C12N9/42 ,  C12P21/04 ,  D06L1/06 ,  D06M16/00 ,  C11D7/42 ,  C12N9/28

    CPC分类号： D06M16/003 ,  C12N9/20 ,  C12N9/2414 ,  C12N9/2437 ,  C12Y302/01001 ,  C12Y302/01004 ,  D06L1/06 ,  C07K2319/00

    摘要： Cellulose-containing fabric is desized by treating with an enzyme hybrid having a catalytically active amino acid sequence of an enzyme such as a lipase or an amylase linked to an amino acid sequence containing a cellulose-binding domain. The enzyme amino acid sequence may be of an .alpha.-amylase obtainable from a species of Bacillus such as Bacillus licheniformis, or of a lipase obtainable from a species of Humicola, Candida, Pseudomonas or Bacillus. The cellulose-binding domain may be from a cellulase, a xylanase, a mannanase, an arabinofuranosidase, an acetylesterase or a chitinase. The enzyme hybrid is obtained from a transformed host cell containing an expression cassette having a DNA sequence encoding the enzyme hybrid. A desizing composition is formed containing the enzyme hybrid and a wetting agent.

    摘要翻译： 含有纤维素的织物通过用具有酶的催化活性氨基酸序列的酶杂交处理，如与包含纤维素结合结构域的氨基酸序列连接的脂肪酶或淀粉酶。 酶氨基酸序列可以是可从诸如地衣芽孢杆菌的芽孢杆菌种类获得的α-淀粉酶，或可从Humicola，假丝酵母​​属，假单胞菌属或芽孢杆菌属种获得的脂肪酶。 纤维素结合结构域可以来自纤维素酶，木聚糖酶，甘露聚糖酶，阿拉伯呋喃糖苷酶，乙酰酯酶或几丁质酶。 从具有编码酶杂交体的DNA序列的表达盒的转化宿主细胞获得酶杂交体。 形成含有酶杂化物和润湿剂的脱浆组合物。