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公开(公告)号：US4101450A
公开(公告)日：1978-07-18
申请号：US795260
申请日：1977-05-09
申请人： H. Shinn Hwang , Paul D. Taylor
发明人： H. Shinn Hwang , Paul D. Taylor
CPC分类号： C07C29/158 , B01J23/464 , C07C51/10 , Y02P20/52 , Y10S502/52
摘要： This invention provides a novel rhodium-ruthenium catalyst composition which is adapted for highly selective conversion of synthesis gas to acetic acid and related oxygenated two-carbon hydrocarbon derivatives.
摘要翻译： 本发明提供了一种新的铑 - 钌催化剂组合物，其适用于将合成气高度选择性转化为乙酸和相关的含氧二碳烃衍生物。
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82.发明授权Process for making copolymers of maleic anhydride and alkyl vinyl ether of high specific viscosity 有权制备高粘度马来酸酐和烷基乙烯基醚的共聚物的方法公开(公告)号：US07396886B2
公开(公告)日：2008-07-08
申请号：US11158126
申请日：2005-06-21
申请人： Yoon Tae Kwak , Paul D. Taylor , William J. Swatos
发明人： Yoon Tae Kwak , Paul D. Taylor , William J. Swatos
IPC分类号： C08F138/02
CPC分类号： C08F222/06 , C08F2/38 , C08F216/18 , Y10S526/916
摘要： A process for making high specific viscosity copolymers of maleic anhydride and an alkyl vinyl either having as specific viscosity of at least 7, preferably 10-50, are made by copolymerizing maleic anhydride and an alkly vinyl ether monomers in the presence of added acetylene.
摘要翻译： 通过在添加的乙炔的存在下使马来酸酐和烷基乙烯基醚单体共聚，制备马来酸酐和具有至少7，优选10-50的比粘度的马来酸酐和烷基乙烯基的高比粘度共聚物的方法。
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83.发明授权公开(公告)号：US07225079B2
公开(公告)日：2007-05-29
申请号：US10308576
申请日：2002-12-02
CPC分类号： C12Q1/6827 , B01D15/166 , B01D15/366 , C12N15/101 , C12Q1/6869 , G01N30/30 , G01N30/96 , G01N2030/8827 , C12Q2565/137 , C12Q2527/107
摘要： In an extensive Matched Ion Polynucleotide Chromatography (MIPC) system and method, and the computer programs or software associated therewith, the system provides automated options for sample selection, mobile phase gradient selection and control, column and mobile phase temperature control, and fragment collection for a wide variety of MIPC separation processes. MIPC separation processes can be applied to effect size-based separation of DNA fragments, mutation detection, DNA fragment purification, PCR process monitoring and other novel processes. This invention is directed to the system and software which automates many of these procedures, facilitating use of the system to achieve complex separation methods. In one embodiment of the invention, a user specifies a size range of double stranded DNA fragment(s) in a mixture, the software calculates a solvent gradient to elute the fragment(s), and the system performs the chromatographic separation using the calculated gradient. In an embodiment useful in DNA mutation detection, a user specifies the base sequence of a wild type DNA molecule, the software calculates a temperature for partially denaturing heteroduplex and homoduplex molecules of the DNA in a mixture, the software calculates a solvent gradient to elute the fragments, and the system performs the chromatographic separation using the calculated gradient and temperature.
摘要翻译： 在广泛的匹配离子多核苷酸色谱（MIPC）系统和方法以及与其相关的计算机程序或软件中，系统提供用于样品选择，流动相梯度选择和控制，色谱柱和流动相温度控制以及片段收集的自动选择 各种MIPC分离过程。 可以应用MIPC分离过程来实现基于大小的DNA片段分离，突变检测，DNA片段纯化，PCR过程监控等新方法。 本发明涉及自动化许多这些程序的系统和软件，便于使用该系统来实现复杂的分离方法。 在本发明的一个实施方案中，用户指定混合物中双链DNA片段的大小范围，软件计算溶剂梯度以洗脱片段，并且系统使用计算的梯度进行色谱分离 。 在DNA突变检测中有用的实施方案中，用户指定野生型DNA分子的碱基序列，软件计算混合物中DNA的部分变性异源双链体和同源双链体分子的温度，软件计算溶剂梯度洗脱 片段，系统使用计算的梯度和温度进行色谱分离。
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公开(公告)号：US07138518B1
公开(公告)日：2006-11-21
申请号：US09714579
申请日：2000-11-16
申请人： Douglas T. Gjerde , Robert M. Haefele , Paul D. Taylor , Christopher P. Hanna , Alezander I. Kuklin , David P. Hornby
发明人： Douglas T. Gjerde , Robert M. Haefele , Paul D. Taylor , Christopher P. Hanna , Alezander I. Kuklin , David P. Hornby
CPC分类号： C12N15/101
摘要： In one aspect, the invention provides a method for separating a mixture of polynucleotides, such as DNA or RNA, including (a) applying the mixture to a polymeric separation medium having non-polar surfaces, wherein the surfaces are characterized by being substantially free from multivalent cations, such as metal ions, which are free to interfere with polynucleotide separation, and (b) eluting the mixture with a mobile phase containing organic solvent and counter ion agent. In the separation of single-stranded polynucleotides, improved separation is obtained at a temperature effective to fully denature secondary structure within the polynucleotides.
摘要翻译： 一方面，本发明提供了分离多核苷酸（例如DNA或RNA）的混合物的方法，包括（a）将该混合物施用于具有非极性表面的聚合物分离介质，其中所述表面的特征在于基本上不含 可自由干扰多核苷酸分离的多价阳离子，例如金属离子，和（b）用含有机溶剂和抗衡离子剂的流动相洗脱该混合物。 在单链多核苷酸的分离中，在有效使多核苷酸内完全变性二级结构的温度下获得改进的分离。
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85.发明授权Detection of nucleic acid heteroduplex molecules by anion-exchange chromatography 失效通过阴离子交换层析检测核酸异源双链分子公开(公告)号：US06969587B2
公开(公告)日：2005-11-29
申请号：US09756070
申请日：2001-01-06
申请人： Paul D. Taylor
发明人： Paul D. Taylor
CPC分类号： B01J41/20 , B01D15/366 , C12Q1/6827 , C12Q2565/137
摘要： The present invention describes a method for separating or partially separating heteroduplex and homoduplex DNA molecules in a mixture. In the method, the mixture is applied to an anion-exchange chromatography medium. The heteroduplex and homoduplex molecules are eluted with a mobile phase containing an eluting salt, including an anion and a cation, a buffer, and preferably including an organic solvent. The eluting is carried out under conditions effective to at least partially denature the heteroduplexes (e.g., thermal or chemical denaturing) resulting in the separation of the heteroduplexes from the homoduplexes. The method has many applications including, but not limited to, detecting mutations and comparative DNA sequencing.
摘要翻译： 本发明描述了在混合物中分离或部分分离异源双链体和同源双链DNA分子的方法。 在该方法中，将混合物施加到阴离子交换色谱介质上。 异源二聚体和同源双链分子用含有洗脱盐的流动相洗脱，包括阴离子和阳离子，缓冲液，优选包括有机溶剂。 洗脱在有效地使异源双链体（例如，热或化学变性）至少部分变性的条件下进行，导致异源双链体与同源双链体分离。 该方法具有许多应用，包括但不限于检测突变和比较DNA测序。
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公开(公告)号：US06642374B2
公开(公告)日：2003-11-04
申请号：US09764041
申请日：2001-01-16
IPC分类号： C07H2100
CPC分类号： C12Q1/6806 , B01D15/366 , B01J20/26 , B01J20/264 , B01J20/28004 , B01J20/286 , B01J20/3092 , B01J20/3204 , B01J20/321 , B01J20/3227 , B01J20/3246 , B01J20/3268 , B01J2220/54 , B01J2220/58 , C12N15/101
摘要： A batch process for obtaining polynucleotide fragments, such as dsDNA, having a selected size from a mixture of polynucleotide fragments including the steps of a) applying a solution of the mixture of polynucleotide fragments and a counterion agent to a binding medium having a hydrophobic surface; b) contacting the binding medium with a first stripping solvent and counterion agent, the first stripping solvent having a concentration of organic component sufficient to release from the binding medium all polynucleotide fragments having a size smaller than the selected size, and removing the first stripping solvent from the binding medium; and c) contacting the binding medium with a second stripping solvent having a concentration of organic component sufficient to release from the binding medium the polynucleotide fragments having the selected size, and removing the second stripping solvent from the binding medium. The binding medium can be organic polymer or inorganic particle beads. The mixture of polynucleotides can be the product of a PCR amplification. The binding medium can be contained within a column, a web or a container.
摘要翻译： 用于从多核苷酸片段的混合物中获得具有选定大小的多核苷酸片段（例如dsDNA）的分批方法，包括以下步骤：a）将多核苷酸片段和抗衡离子混合物的溶液施用于具有疏水性表面的结合介质; b）使所述结合介质与第一剥离溶剂和抗衡离子剂接触，所述第一剥离溶剂具有足以从所述结合介质释放的有机组分的浓度，所述多个片段的尺寸小于所选择的尺寸，并且除去所述第一剥离溶剂 从结合介质; 和c）使所述结合介质与第二剥离溶剂接触，所述第二剥离溶剂具有足以从所述结合介质释放具有所选择尺寸的多核苷酸片段的有机组分浓度，以及从所述结合介质中除去所述第二剥离溶剂。 结合介质可以是有机聚合物或无机颗粒珠。 多核苷酸的混合物可以是PCR扩增的产物。 结合介质可以包含在柱，网或容器中。
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公开(公告)号：US06287822B1
公开(公告)日：2001-09-11
申请号：US09129105
申请日：1998-08-04
IPC分类号： C12P1934
CPC分类号： C12Q1/6806 , C12Q1/6827 , C12Q2565/137 , C12Q2527/107
摘要： The present invention is directed to improved methods for detection of mutations in DNA using Denaturing Matched Ion Polynucleotide Chromatography (DMIPC). The invention includes the following aspects: analysis of PCR amplification products to identify factors that affect PCR replication fidelity; design of PCR primers; selection of an optimal temperature for performing DMIPC; selection of the mobile phase composition for gradient elution; methods for column preparation and maintenance; and methods for preparing polynucleotide samples prior to chromatographic analysis.
摘要翻译： 本发明涉及使用变性匹配离子多核苷酸色谱（DMIPC）检测DNA突变的改进方法。 本发明包括以下几个方面：分析PCR扩增产物，鉴定影响PCR复制保真度的因素; PCR引物设计; 选择用于执行DMIPC的最佳温度; 选择流动相组成进行梯度洗脱; 柱准备和维护方法; 以及在色谱分析之前制备多核苷酸样品的方法。
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公开(公告)号：US06177559B1
公开(公告)日：2001-01-23
申请号：US09391963
申请日：1999-09-08
IPC分类号： C07H2100
CPC分类号： C12Q1/6806 , B01D15/366 , B01J20/26 , B01J20/264 , B01J20/28004 , B01J20/286 , B01J20/3092 , B01J20/3204 , B01J20/321 , B01J20/3227 , B01J20/3246 , B01J20/3268 , B01J2220/54 , B01J2220/58 , C12N15/101
摘要： A batch process for obtaining polynucleotide fragments, such as dsDNA, having a selected size from a mixture of polynucleotide fragments including the steps of a) applying a solution of the mixture of polynucleotide fragments and a counterion agent to a binding medium having a hydrophobic surface; b) contacting the binding medium with a first stripping solvent and counterion agent, the first stripping solvent having a concentration of organic component sufficient to release from the binding medium all polynucleotide fragments having a size smaller than the selected size, and removing the first stripping solvent from the binding medium; and c) contacting the binding medium with a second stripping solvent having a concentration of organic component sufficient to release from the binding medium the polynucleotide fragments having the selected size, and removing the second stripping solvent from the binding medium. The binding medium can be organic polymer or inorganic particle beads. The mixture of polynucleotides can be the product of a PCR amplification. The binding medium can be contained within a column, a web or a container.
摘要翻译： 用于从多核苷酸片段的混合物中获得具有选定大小的多核苷酸片段（例如dsDNA）的分批方法，包括以下步骤：a）将多核苷酸片段和抗衡离子混合物的溶液施用于具有疏水性表面的结合介质; b）使所述结合介质与第一剥离溶剂和抗衡离子剂接触，所述第一剥离溶剂具有足以从所述结合介质释放的有机组分的浓度，所述多个片段的尺寸小于所选择的尺寸，并且除去所述第一剥离溶剂 从结合介质; 和c）使所述结合介质与第二剥离溶剂接触，所述第二剥离溶剂具有足以从所述结合介质释放具有所选择尺寸的多核苷酸片段的有机组分浓度，以及从所述结合介质中除去所述第二剥离溶剂。 结合介质可以是有机聚合物或无机颗粒珠。 多核苷酸的混合物可以是PCR扩增的产物。 结合介质可以包含在柱，网或容器中。
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公开(公告)号：US06136195A
公开(公告)日：2000-10-24
申请号：US285331
申请日：1999-04-02
IPC分类号： B01D15/08
CPC分类号： B01D15/366 , B01D15/203 , G01N30/50 , G01N2030/201 , G01N2030/202
摘要： An apparatus for effecting base pair length separations of DNA fragments by matched ion paired chromatography comprising a separation column containing separation media having non-polar DNA separation surfaces, separation solution supply means, and a separation solution conduit communicating with the separation column and the separation solution supply means, and a cleaning solution valve means positioned in the separation solution conduit for injecting cleaning solution into the separation solution conduit. A process for cleaning the non-polar DNA separation surfaces in the apparatus comprising interrupting the flow of separation solvent with a block of cleaning solution injected into the flow of separation solution passing to the column, the cleaning solution containing agent which removes accumulated residues from the non-polar surface. The cleaning solution can have an alkaline pH and contain a chelating agent such as EDTA.
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公开(公告)号：US6056877A
公开(公告)日：2000-05-02
申请号：US183450
申请日：1998-10-30
申请人： Douglas T. Gjerde , Paul D. Taylor
发明人： Douglas T. Gjerde , Paul D. Taylor
IPC分类号： B01D15/08
CPC分类号： B01D15/325 , B01D15/366 , B01J20/287
摘要： Nonporous beads having an average diameter of about 0.5-100 microns are suitable for chromatographic separation of mixtures of polynucleotides when the beads comprise a nonporous particle which are coated with a polymer or which have substantially all surface substrate groups endcapped with a non-polar hydrocarbon or substituted hydrocarbon group. The beads provide efficient separation of polynucleotides using Matched Ion Polynucleotide Chromatography.
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