公开(公告)号：US10851135B2

公开(公告)日：2020-12-01

申请号：US16796009

申请日：2020-02-20

Applicant: UNIVERSITY OF WASHINGTON

Inventor： Robert A. Langan ,  Scott Boyken ,  David Baker ,  Walter Novak ,  Marc Joseph Lajoie ,  Alfredo Quijano Rubio

IPC: C07K14/00 ,  A61K47/69

Abstract: Disclosed are protein switches that can sequester bioactive peptides and/or binding domains, holding them in an inactive (“off”) state, until combined with a second designed polypeptide called the key, which induces a conformational change that activates (“on”) the bioactive peptide or binding domain, components of such protein switches, and their use.

公开(公告)号：US10818377B2

公开(公告)日：2020-10-27

申请号：US15813872

申请日：2017-11-15

Applicant: University of Washington

Inventor： David Baker ,  Jorge Fallas ,  George Ueda ,  William H. Sheffler ,  Vanessa Nguyen

IPC: C07K14/00 ,  G16B5/00 ,  A61K38/03 ,  G16B15/30 ,  A61K38/00

Abstract: Described herein are polypeptides capable of self-assembling to form homo-oligomers, and methods for designing such polypeptides.

公开(公告)号：US10793846B2

公开(公告)日：2020-10-06

申请号：US15575159

申请日：2016-06-08

Applicant: UNIVERSITY OF WASHINGTON ,  THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： Ingrid Swanson Pultz ,  Clancey Wolf ,  Justin Bloomfield Siegel ,  Christine Elaine Tinberg ,  Lance Stewart ,  David Baker

IPC: C12N9/52 ,  A61K38/00 ,  A61P1/14 ,  C12N9/50

Abstract: Polypeptides, and methods for their use, are disclosed that have an amino acid sequence at least 75% identical to the amino acid sequence of SEQ ID NO: 1, are provided, wherein (a) the polypeptide degrades a PFQPQLPY (SEQ ID NO: 140) peptide and/or a PFPQPQQPF (SEQ ID NO: 68) at pH 4; (b) residue 467 is Ser, residue 267 is Glu, and residue 271 is Asp; and (c) the polypeptide comprises an amino acid change from SEQ ID NO: 1 at one or more residues selected from the group consisting of 221, 262E, 268, 269, 270, 319A, 320, 354E/Q/R/Y, 358S/Q/T, 368F/Q, 399, 402, 406, 424, 449, 461, 463, 105, 171, 172, 173, 174, and 456.

公开(公告)号：US20190155988A2

公开(公告)日：2019-05-23

申请号：US15813872

申请日：2017-11-15

Applicant: University of Washington

Inventor： David Baker ,  Jorge Fallas ,  George Ueda ,  William H. Sheffler ,  Vanessa Nguyen

IPC: G06F19/12 ,  A61K38/03

CPC classification number: G16B5/00 ,  A61K38/03 ,  C07K14/00 ,  G16B15/30

Abstract: Described herein are polypeptides capable of self-assembling to form homo-oligomers, and methods for designing such polypeptides.

公开(公告)号：US20180228842A1

公开(公告)日：2018-08-16

申请号：US15916689

申请日：2018-03-09

Applicant: The Board of Trustees of the Leland Stanford Junior University ,  UNIVERSITY OF WASHINGTON

Inventor： Kenan Christopher Garcia ,  Jonathan Sockolosky ,  David Baker ,  Chris King

IPC: A61K35/17 ,  C12N5/0783 ,  C12N15/10 ,  A61K38/17 ,  A61K38/20

Abstract: Engineered orthogonal cytokine receptor/ligand pairs, and methods of use thereof, are provided.

公开(公告)号：US09925249B2

公开(公告)日：2018-03-27

申请号：US15633065

申请日：2017-06-26

Applicant: UNIVERSITY OF WASHINGTON THROUGH ITS CENTER FOR COMMERCIALIZATION

Inventor： Justin Siegel ,  David Baker ,  Sydney Rin Anna Gordon ,  Ingrid Swanson Pultz ,  Elizabeth Joy Stanley ,  Sarah Jane Wolf

IPC: A61K38/48 ,  C12N9/50 ,  C12N9/52

CPC classification number: A61K38/482 ,  A61K38/48 ,  C12N9/50 ,  C12N9/52 ,  C12Y304/21

Abstract: The invention provides compositions and methods for treating celiac sprue.

公开(公告)号：US09771395B2

公开(公告)日：2017-09-26

申请号：US15120071

申请日：2015-03-20

Applicant: UNIVERSITY OF WASHINGTON

Inventor： Merika Treants Koday ,  Deborah L. Fuller ,  Aaron Chevalier ,  Jorgen Nelson ,  David Baker

IPC: A61K38/00 ,  C07K14/00 ,  G01N33/569 ,  A61K9/00

CPC classification number: C07K14/00 ,  A61K9/0043 ,  A61K38/00 ,  G01N33/56983 ,  G01N2333/11 ,  G01N2500/04

Abstract: Designed polypeptides having the amino acid sequence of SEQ ID NQ: 1 are described that bind with high affinity and selectivity to the influenza hemagglutinin protein, and which can be used for treating and/or limiting an influenza infection, as well as diagnosing an influenza infection and identifying candidate compounds for treating an influenza infection.

公开(公告)号：US20160202256A1

公开(公告)日：2016-07-14

申请号：US14993509

申请日：2016-01-12

Applicant: President and Fellows of Harvard College ,  University of Washington

Inventor： George M. Church ,  Justin Feng ,  Daniel J. Mandell ,  David Baker ,  Stanley Fields ,  Benjamin Ward Jester ,  Christine Elaine Tinberg

IPC: G01N33/566 ,  G01N33/74

CPC classification number: G01N33/743 ,  G01N2500/10

Abstract: Disclosed is a biosensor engineered to conditionally respond to the presence of specific small molecules, the biosensors including conditionally stable ligand-binding domains (LBDs) which respond to the presence of specific small molecules, wherein readout of binding is provided by reporter genes or transcription factors (TFs) fused to the LBDs.

Abstract translation: 公开了一种工程化以有条件地响应特定小分子的存在的生物传感器，生物传感器包括响应特定小分子存在的条件稳定的配体结合结构域（LBD），其中结合的读出由报告基因或转录因子 （TF）与LBD融合。

公开(公告)号：US09181300B2

公开(公告)日：2015-11-10

申请号：US14221346

申请日：2014-03-21

Applicant: University of Washington through its Center for Commercialization

Inventor： David Baker ,  Timothy A. Whitehead ,  Sarel Fleishman

IPC: A61K38/00 ,  A61K38/10 ,  C07K14/00 ,  C07K7/08 ,  G01N33/569 ,  G01N33/53

CPC classification number: C07K7/08 ,  A61K38/00 ,  A61K38/10 ,  A61K38/16 ,  C07K14/00 ,  C07K14/001 ,  G01N33/53 ,  G01N33/56983 ,  G01N2333/11 ,  G01N2469/10

Abstract: Polypeptides are disclosed herein, which recognize and are strong binders to Influenza A hemagglutinin and can be used, for example, to treat and/or limit development of an influenza infection.

Abstract translation: 本文公开了多肽，其识别并且是甲型流感血凝素的强结合剂，并且可以用于例如治疗和/或限制流感感染的发展。

公开(公告)号：US20150038408A1

公开(公告)日：2015-02-05

申请号：US14384901

申请日：2013-03-12

Applicant: University of Washington through its Center for Commercialization

Inventor： David Baker ,  Timothy A. Whitehead ,  Sarel Fleishman

IPC: C07K14/00 ,  G01N33/569 ,  C07K7/08

CPC classification number: C07K14/00 ,  A61K38/00 ,  C07K7/08 ,  G01N33/56983 ,  G01N2333/11

Abstract: The present invention provides polypeptides that recognize and are strong binders to Influenza A hemagglutinin and can be used, for example, to treat and/or limit development of an influenza infection. The present invention further provides isolated nucleic acids encoding the polypeptides of the invention, recombinant expression vectors comprising the nucleic acids encoding the polypeptides of the invention operatively linked to a suitable control sequence, and recombinant host cells comprising the recombinant expression vectors of the invention. The present invention also provides antibodies that selectively bind to the polypeptides of the invention, and pharmaceutical compositions comprising one or more polypeptides according to the invention and a pharmaceutically acceptable carrier. Additionally, the present invention provides methods for treating and/or limiting an influenza infection, methods for diagnosing an influenza infection, or monitoring progression of an influenza infection, methods for identifying candidate influenza vaccines, and methods for identifying candidate compounds for treating, limiting, and/or diagnosing influenza infection.

Abstract translation: 本发明提供识别并且是甲型流感血凝素的强粘合剂的多肽，并且可以用于例如治疗和/或限制流感感染的发展。 本发明还提供了编码本发明多肽的分离的核酸，包含编码可操作地连接到合适的控制序列的本发明的多肽的核酸的重组表达载体和包含本发明的重组表达载体的重组宿主细胞。 本发明还提供了选择性结合本发明的多肽的抗体，以及包含一种或多种本发明多肽和药学上可接受的载体的药物组合物。 另外，本发明提供了用于治疗和/或限制流感感染的方法，用于诊断流感感染的方法或监测流感感染的进展，用于鉴定候选流感疫苗的方法，以及用于鉴定候选化合物的方法，用于治疗，限制， 和/或诊断流感感染。