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    IN VITRO METHOD FOR ESTIMATING IN VIVO PROTEIN DIGESTIBILITY
    85.
    发明申请
    IN VITRO METHOD FOR ESTIMATING IN VIVO PROTEIN DIGESTIBILITY 有权
    用于估计体外蛋白质可溶性的体外方法

    公开(公告)号：US20160208309A1

    公开(公告)日：2016-07-21

    申请号：US14599050

    申请日：2015-01-16

    申请人： General Mills, Inc.

    发明人： David W. Plank

    IPC分类号： C12Q1/37 ,  G01N21/75

    CPC分类号： C12Q1/37 ,  G01N21/75 ,  G01N33/02 ,  G01N33/6806

    摘要： An analysis technique can be performed to quantify the digestible protein content of a protein-containing sample outside the body of a living organism. Traditionally, protein digestibility is evaluated in vivo, for example using a rat subject to measure protein digestibility after being fed the protein-containing sample. In some examples, an in vitro technique involves enzymatically digesting the protein-containing sample to simulate digestion that would occur inside a mammalian body. The sample can then be optically analyzed to measure the amount of reactive amine present in the sample, which can provide an indication of the amount of amino acid released during digestion. In some examples, the measured reactive amine value is adjusted to account for the stronger and/or weaker optical response of certain amino acids due to their relative reactivity with an optical tagging agent. Thereafter, an in vivo protein digestibility value can be calculated based on adjusted amine concentration.

    摘要翻译： 可以进行分析技术来定量活体外的蛋白质样品的可消化蛋白质含量。 传统上，蛋白质消化率在体内进行评估，例如使用大鼠饲喂含有蛋白质的样品后测量蛋白质消化率。 在一些实例中，体外技术涉及酶消化含蛋白质的样品以模拟哺乳动物体内发生的消化。 然后可以对样品进行光学分析以测量样品中存在的反应性胺的量，其可以提供消化期间释放的氨基酸的量的指示。 在一些实例中，测量的反应性胺值被调整以考虑到由于其与光学标签试剂的相对反应性而导致的某些氨基酸的更强和/或更弱的光学响应。 此后，可以基于调节的胺浓度计算体内蛋白质消化率值。

    STANDARD SOLUTION FOR USE IN ANALYSIS OF AMINO ACID IN PLASMA
    87.
    发明申请
    STANDARD SOLUTION FOR USE IN ANALYSIS OF AMINO ACID IN PLASMA 有权
    在等离子体中氨基酸分析中使用的标准溶液

    公开(公告)号：US20150064792A1

    公开(公告)日：2015-03-05

    申请号：US14232073

    申请日：2012-07-19

    申请人： Masako Hayakawa ,  Kazutaka Shimbo ,  Hiroo Yoshida

    发明人： Masako Hayakawa ,  Kazutaka Shimbo ,  Hiroo Yoshida

    IPC分类号： G01N33/96 ,  G01N33/68

    CPC分类号： G01N33/96 ,  G01N33/6806 ,  G01N33/6848 ,  G01N2030/042 ,  G01N2496/80 ,  Y10T436/105831 ,  Y10T436/106664

    摘要： An external standard solution for use in the analysis of amino acid in plasma, containing, (1) at least one amino acid selected from the following components A, at a concentration of 0.0007 M to 0.49 M, and (2) (i) at least one amino acid selected from the following components B, at a concentration of 0.2 to 0.9 times of the lowest-concentration amino acid among the amino acids selected from components A, (ii) at least one amino acid selected from the following components C, at a concentration of 0.1 to 0.4 times of the lowest-concentration amino acid among amino acids selected from the components A, or (iii) at least one amino acid selected from the following components D, at a concentration of 0.05 to 0.2 times of the lowest-concentration amino acid among amino acids selected from the components A, [Components A] valine, glycine, alanine and glutamine [Components B] serine, proline, threonine, taurine, leucine, isoleucine, lysine, histidine, phenylalanine and tyrosine [Components C] asparagine, ornithine, arginine and tryptophan [Components D] glutamic acid, methionine, citrulline and cystine.

    摘要翻译： 用于血浆中氨基酸分析的外标溶液，其含有（1）选自以下组分A中的至少一种氨基酸，浓度为0.0007M至0.49M，和（2）（i） 选自以下组分B中的至少一种氨基酸，选自组分A的氨基酸中浓度为最低浓度氨基酸的0.2至0.9倍，（ii）至少一种选自以下组分C， 浓度为选自组分A的氨基酸中的最低浓度氨基酸的0.1至0.4倍，或（iii）至少一种选自以下组分D的氨基酸，浓度为0.05至0.2倍 选自组分A，[组分A]缬氨酸，甘氨酸，丙氨酸和谷氨酰胺的氨基酸中的最低浓度氨基酸[组分B]丝氨酸，脯氨酸，苏氨酸，牛磺酸，亮氨酸，异亮氨酸，赖氨酸，组氨酸，苯丙氨酸和酪氨酸[组分 C] 天冬酰胺，鸟氨酸，精氨酸和色氨酸[组分D]谷氨酸，甲硫氨酸，瓜氨酸和胱氨酸。

    Fructosyl amino acid oxidase
    90.
    发明授权
    Fructosyl amino acid oxidase 有权
    果糖基氨基酸氧化酶

    公开(公告)号：US08497083B2

    公开(公告)日：2013-07-30

    申请号：US13365487

    申请日：2012-02-03

    申请人： Kazunori Ikebukuro ,  Sode Koji

    发明人： Kazunori Ikebukuro ,  Sode Koji

    IPC分类号： C12Q1/26 ,  C12Q1/00 ,  C12N11/00 ,  C12N9/02

    CPC分类号： C12N9/0022 ,  C12Q1/005 ,  G01N33/6803 ,  G01N33/6806 ,  G01N33/723

    摘要： In one form, a mutant fructosyl amino acid oxidase modified at an amino acid residue involved in a proton relay system is provided. The mutant fructosyl amino acid oxidase has reduced oxidase activity while substantially maintaining its dehydrogenase activity. Other forms include an assay device and assay method for measuring glycated protein. Still, other forms include unique methods, techniques, systems and devices involving a mutant fructosyl amino acid oxidase.

    摘要翻译： 在一种形式中，提供了在涉及质子中继系统的氨基酸残基处修饰的突变果糖基氨基酸氧化酶。 突变型果糖基氨基酸氧化酶具有降低的氧化酶活性，同时基本维持其脱氢酶活性。 其他形式包括用于测量糖基化蛋白质的测定装置和测定方法。 仍然，其它形式包括涉及突变果糖基氨基酸氧化酶的独特方法，技术，系统和装置。