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26 条记录 (耗时 0.026 秒)

    DETECTION SYSTEM
    2.
    发明申请
    DETECTION SYSTEM 审中-公开
    检测系统

    公开(公告)号：US20090068672A1

    公开(公告)日：2009-03-12

    申请号：US12279088

    申请日：2007-02-16

    申请人： Martin Alan Lee ,  Tom Brown ,  Diane Rachel Sutton ,  Mark Andrew Laverick

    发明人： Martin Alan Lee ,  Tom Brown ,  Diane Rachel Sutton ,  Mark Andrew Laverick

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6818 ,  C12Q2563/173 ,  C12Q2565/101

    摘要： The use of a red nucleic acid stain, in particular red fluorescent SYTO® dye in various methods used for the detection or characterisation of nucleic acids is described. In particular, the red nucleic acid stains have been found to be particularly compatible with the polymerase chain reaction (PCR), and therefore form the basis of enhanced detection methods.

    摘要翻译： 描述了用于检测或表征核酸的各种方法中使用红色核酸染色剂，特别是红色荧光SYTO（R）染料。 特别地，已经发现红色核酸污渍与聚合酶链反应（PCR）特别相容，因此形成增强检测方法的基础。

    NUCLEIC ACID EXTRACTION METHOD
    4.
    发明申请
    NUCLEIC ACID EXTRACTION METHOD 审中-公开
    核酸提取方法

    公开(公告)号：US20130225800A1

    公开(公告)日：2013-08-29

    申请号：US13820186

    申请日：2011-09-01

    申请人： David Squirrell

    发明人： David Squirrell

    IPC分类号： C12N15/10

    CPC分类号： C12N15/1013

    摘要： There is provided a method of extracting a nucleic acid analyte from a cell or virus in a sample chamber, comprising a) adding disruption beads comprising external silica or glass to the sample chamber; b) agitating the disruption beads within the sample chamber to disrupt the cell; c) adding binding particles comprising external silica or glass to the sample chamber in the presence of a chaotropic agent; d) contacting the contents of the sample chamber with a removal device with which the binding particles reversibly associate; and e) separating the removal device and associated binding particles from the sample chamber, thereby removing the nucleic acid analyte from the sample. There are also provided apparatus and kits for use with the method.

    摘要翻译： 提供了从样品室中的细胞或病毒中提取核酸分析物的方法，其包括：a）向样品室中加入包含外部二氧化硅或玻璃的破碎珠粒; b）搅拌样品室内的破碎珠破坏细胞; c）在离液剂的存在下，向样品室中加入包含外部二氧化硅或玻璃的结合颗粒; d）将样品室的内容物与结合颗粒可逆地结合的去除装置接触; 以及e）从所述样品室分离所述去除装置和相关联的结合颗粒，从而从所述样品中除去所述核酸分析物。 还提供了用于该方法的装置和套件。

    NUCLEIC ACID AMPLIFICATION EMPLOYING TEMPERATURE OSCILLATION
    5.
    发明申请
    NUCLEIC ACID AMPLIFICATION EMPLOYING TEMPERATURE OSCILLATION 审中-公开
    核酸放大使用温度振荡

    公开(公告)号：US20120208192A1

    公开(公告)日：2012-08-16

    申请号：US13394098

    申请日：2010-09-08

    申请人： Martin Lee ,  Andrea Hamilton

    发明人： Martin Lee ,  Andrea Hamilton

    IPC分类号： C12Q1/68 ,  G01N21/64 ,  C12M1/40 ,  G01N21/76

    CPC分类号： C12Q1/686 ,  C12Q1/6865 ,  C12Q2527/113 ,  C12Q2527/101 ,  C12Q2521/119

    摘要： A method for carrying out an isothermal nucleic acid amplification reaction at a predetermined temperature, said method comprising changing the temperature of the reaction mixture away from the said predetermined temperature and allowing it to return to the predetermined temperature at least once during the amplification reaction so as to cause a temperature oscillation in particular of up to 15° C., for example of from 2-10° C. Apparatus adapted to carry out the method forms a further aspect of the invention.

    摘要翻译： 一种在预定温度下进行等温核酸扩增反应的方法，所述方法包括将反应混合物的温度从所述预定温度改变，并使其在扩增反应期间至少返回到预定温度，以便 导致温度振荡，特别是高达15℃，例如2-10℃。适于实施该方法的设备形成本发明的另一方面。

    APPARATUS AND METHOD FOR CALIBRATION OF NON-CONTACT THERMAL SENSORS
    6.
    发明申请
    APPARATUS AND METHOD FOR CALIBRATION OF NON-CONTACT THERMAL SENSORS 审中-公开
    用于校准非接触式热传感器的装置和方法

    公开(公告)号：US20120003726A1

    公开(公告)日：2012-01-05

    申请号：US12673210

    申请日：2008-08-15

    申请人： Ross Peter Jones ,  David James Squirrell

    发明人： Ross Peter Jones ,  David James Squirrell

    IPC分类号： C12M1/40 ,  G01K15/00 ,  G01N25/00

    CPC分类号： B01L7/52 ,  B01L3/508 ,  B01L3/50851 ,  B01L2200/147 ,  B01L2200/148 ,  B01L2300/0654 ,  B01L2300/0838 ,  B01L2300/1827 ,  B01L2300/1838 ,  B01L2300/1844

    摘要： Biochemical assay apparatus uses a container with a sleeve of electrically-conductive material (300) to heat it. The heating is done inside a chamber and a contactless heat sensor (110) such as a thermopile or a bolometer, also inside the chamber, is used to monitor the temperature of the electrically conductive material (300). There are many factors that distort the output of the heat sensor (110), particularly as the temperature rises and properties such as emissivity change, or as time goes by and tarnishing and dust affect the heat sensor output. Because the sleeve has low thermal mass and heat transfer only has to happen over short distances, it is relatively easy to calculate a change in actual temperature of the electrically conductive material (300) when subjected to a known pulse of drive current and this property can be used to calibrate the performance of the heat sensor (110) in situ in the chamber.

    摘要翻译： 生物化学测定装置使用具有导电材料套管（300）的容器来加热它。 在室内进行加热，并且还使用室内也使用诸如热电堆或测辐射热计的非接触式热传感器（110）来监测导电材料（300）的温度。 特别是当温度升高并且诸如发射率的特性发生变化，或随着时间的流逝，并且变色和灰尘影响热传感器输出时，存在导致热传感器（110）的输出变形的许多因素。 由于套管具有低的热质量并且传热仅在短距离上发生，所以当经受已知的驱动电流脉冲时，计算导电材料（300）的实际温度变化是相当容易的，并且该性质可以 用于在室内原位校准热​​传感器（110）的性能。

    ANALYTICAL METHOD AND KIT
    7.
    发明申请
    ANALYTICAL METHOD AND KIT 审中-公开
    分析方法和试剂盒

    公开(公告)号：US20110045485A1

    公开(公告)日：2011-02-24

    申请号：US12903666

    申请日：2010-10-13

    申请人： David James Squirrell

    发明人： David James Squirrell

    IPC分类号： C12Q1/68 ,  C07H21/00

    CPC分类号： C12Q1/6816 ,  C12Q1/6872 ,  C12Q2563/103 ,  C12Q2537/143 ,  C12Q2525/117

    摘要： RNA-containing probes and kits comprising RNA-containing probes for the detection and analysis of nucleic acid sequences are described. These probes are contacted with a sample suspected of containing the nucleic acid sequence and if they form duplexes, they are hydrolysed. This may be done, for example during an amplification reaction. AMP generated as a result of the hydrolysis is converted to ATP. The ATP may then be detected using bioluminescent reagents. Inclusion of modified adenosine in at least one probe means that the signal arising from one probe will give rise to a different and distinguishable bioluminescent signal thus enabling the use of for example an internal control in bioluminescently-reported nucleic acid tests.

    摘要翻译： 描述了含RNA探针和包含用于检测和分析核酸序列的含RNA探针的试剂盒。 这些探针与怀疑含有核酸序列的样品接触，如果它们形成双链体，则它们被水解。 这可以例如在扩增反应期间完成。 作为水解产生的AMP被转化为ATP。 然后可以使用生物发光试剂检测ATP。 在至少一个探针中包含修饰的腺苷意味着由一个探针产生的信号将产生不同的且可区分的生物发光信号，从而使得能够在生物发光报道的核酸测试中使用例如内部对照。

    FREEZE-DRIED COMPOSITIONS FOR CARRYING OUT PCR AND OTHER BIOCHEMICAL REACTIONS
    10.
    发明申请
    FREEZE-DRIED COMPOSITIONS FOR CARRYING OUT PCR AND OTHER BIOCHEMICAL REACTIONS 审中-公开
    用于携带PCR和其他生物化学反应的冻干组合物

    公开(公告)号：US20110159497A1

    公开(公告)日：2011-06-30

    申请号：US12664183

    申请日：2008-06-16

    申请人： Martin Alan Lee ,  Jennifer Mitchell ,  Emma Henderson

    发明人： Martin Alan Lee ,  Jennifer Mitchell ,  Emma Henderson

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6806 ,  C12Q2527/125

    摘要： A composition for carrying out a chemical or biochemical reaction, said composition being in a freeze-dried form and comprising (i) a set of reagents comprising at least some of the chemical or biochemical reagents necessary for conducting said chemical or biochemical reaction, (ii) a glass forming agent, (iii) a stabilising agent therefore and (iv) fish gelatine. In particular compositions for carrying out PCR are foreseen. Kits comprising these compositions and methods for using them form a further aspect of the invention.

    摘要翻译： 一种用于进行化学或生物化学反应的组合物，所述组合物为冷冻干燥形式，并且包含（i）一组试剂，其包含进行所述化学或生物化学反应所需的至少一些化学或生物化学试剂，（ii） ），玻璃形成剂，（iii）稳定剂，和（iv）鱼明胶。 特别地，预期用于进行PCR的组合物。 包含这些组合物的组合物和使用它们的方法形成本发明的另一方面。