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公开(公告)号：US20180317459A1
公开(公告)日：2018-11-08
申请号：US15870334
申请日：2018-01-12
发明人： Wei HU , Mouyan JIANG , Yongming LI , Binbin TAO , Ji CHEN , Zuoyan ZHU
IPC分类号： A01K61/17 , C12N15/01 , A61K31/565 , A61K31/167 , A01K61/95 , A01K61/80 , A61D19/02
CPC分类号： A01K61/17 , A01K61/80 , A01K61/95 , A01K67/027 , A01K2207/35 , A01K2217/03 , A01K2227/40 , A01K2267/02 , A61D19/02 , A61K31/167 , A61K31/565 , C12N15/01 , Y02A40/812
摘要： Method for producing YY super-male and XY physiological female common carps, with which YY-chromosome super-male common carp can be cultivated and androgenetic YY super-male common carp is produced, where microsatellite markers are used for paternity testing and test crossing confirmation. The YY common carp is crossed with normal female common carp to produce the progenies of only male common carp, and without sex identification, the juvenile male common carp with known sex are subjected to artificial sex reversal to produce XY physiological female common carp.
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公开(公告)号：US20140331346A1
公开(公告)日：2014-11-06
申请号：US14247145
申请日：2014-04-07
发明人： Wei HU , Yunsheng ZHANG , Jun DAI , Zuoyan ZHU
IPC分类号： C12N15/85 , A01K67/027
CPC分类号： C12N15/8509 , A01K67/0275 , A01K2217/058 , A01K2217/15 , A01K2227/40 , A01K2267/02 , C07K14/461 , C12N2830/002
摘要： The invention discloses a method for controlling fish reproduction, the method comprises the following steps of: A, constructing a recombinant gene CMV-eGFP-SV40-CMV-Ga14-SV40 and establishing a GAL4 transgenic zebrafish line; B, constructing a recombinant gene CMV-RFP-SV40-UAS-antisense dnd and establishing a uas-antisensednd transgenic zebrafish line; and C, hybridizing by taking homozygous transgenic fish of the two lines as parents, and the hybrid generation fish was sterility. The method of the present invention takes a strategy that two fertilizable lines hybridized but hybrid generation is sterility, establishing a novel method with a universal meaning for controlling fish fertility; The method solves the contradiction of the reproductively sterility of fish and the hereditability of characters in a reproduction operation research and can be effectively applied to cultivation of new fish specie and population control.
摘要翻译： 本发明公开了一种鱼繁殖控制方法，该方法包括：构建重组基因CMV-eGFP-SV40-CMV-Ga14-SV40，建立GAL4转基因斑马鱼系; B，构建重组基因CMV-RFP-SV40-UAS反义dnd，建立uas-antensednd转基因斑马鱼系; 和C，通过以两条线的纯合转基因鱼作为亲本杂交，并且杂交代鱼不育。 本发明的方法采取两条可施肥的杂交品系，但是杂交代为不育的策略，建立了一种具有控制鱼类生育能力的普遍意义的新方法; 该方法解决了鱼类繁殖不育与繁殖作业研究中人物遗传性的矛盾，可有效应用于新种鱼种群和群体控制的培育。
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公开(公告)号：US11198846B2
公开(公告)日：2021-12-14
申请号：US16611844
申请日：2018-05-11
发明人： Guitang Wang , Weishan Zhao , Ming Li , Shangong Wu , Hong Zou , Wenxiang Li , Fan Xiong
IPC分类号： A01K67/033 , C12N1/00 , C12N1/10 , C12N5/00
摘要： A medium for culturing Balantidium ctenopharyngodoni in vitro, method for preparing the medium and method for culture in vitro are provided, which belongs to technical field of in vitro culture of intestinal protozoa. The formulation of the culture medium includes: 100 ml of Ringer's solution, 0.5 g of yeast extract, 1.0 g of proteose peptone, 3-6 ml of fetal bovine serum, 6-10 ml of horse serum, 300-500 μl of Bacillus licheniformis suspension, 200-300 mg of aseptic starch. Culture steps include: inoculating collected Balantidium ctenopharyngodoni into a prepared medium, filling with nitrogen and sealing, culturing at 15° C. for 48-72 hours, then transferring to another fresh medium, cycling back and forth, proliferating the Balantidium ctenopharyngodoni continuously. Balantidium ctenopharyngodoni are capable of achieving cell division and proliferation in the culture medium, and lays foundation for the physiological and experimental ecology research of the Balantidium ctenopharyngodoni.
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公开(公告)号：US20210368749A1
公开(公告)日：2021-12-02
申请号：US17215237
申请日：2021-03-29
发明人： Ming DUAN , Weiwei LI , Jianghui BAO , Chaoshuo ZHANG , Xiangyuan MI , Dongxu ZHANG
摘要： A test device for noninvasive testing of fish habitat selection and application thereof is described herein. The device includes a camera device, a water circulating system, a fish transporting barrel, and a test water tank. The fish transporting barrel is a water barrel with an openable bottom. The central area of the test water tank is arranged as a closed adaptation area, a water outlet is formed in the bottom of the adaptation area. Fish passages are formed in the adaptation area. Test areas with equal sizes are formed around the adaptation area through partition plates. A water inlet is formed in each test area. Water flow buffering plates are arranged corresponding to the water inlets. Fish passages are formed in the bottoms of the partition plates of the test areas. The water inlets of the test areas are connected to the water circulating system.
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公开(公告)号：US20210310023A1
公开(公告)日：2021-10-07
申请号：US16905930
申请日：2020-06-19
发明人： Zongbin Cui , Tong Zhou , Bolan Zhou , Yong Long , Guili Song , Qing Li , Zuoyan Zhu
IPC分类号： C12N15/85 , A01K67/027
摘要： A method for preparing a fish skin mucous gland bioreactor and its application, including: identifying genes specifically expressed in fish skin mucinous gland cells, promoters and secreted protein signal peptides, constructing transgenic expression vectors that can specifically express endogenous or heterologous biologically active substances in fish skin and mucous gland cells, developing stable genetic and transgenic fish that secrete bioactive substances into fish mucus, and using bioactive substances secreted by mucus glands for animal and plant growth, stress resistance and disease resistance, human health care and disease prevention, and commercial enzymes. The fish skin mucous gland bioreactor developed by the invention has the characteristics of easy breeding and expansion, more skin mucus secretion, convenient mucus collection, and easy purification of bioactive substances, and can realize the large-scale production of fish skin mucous gland bioreactor and efficient application.
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公开(公告)号：US20210144977A1
公开(公告)日：2021-05-20
申请号：US16611844
申请日：2018-05-11
发明人： Guitang Wang , Weishan Zhao , Ming Li , Shangong Wu , Hong Zou , Wenxiang Li , Fan Xiong
IPC分类号： A01K67/033 , C12N5/00
摘要： A medium for culturing Balantidium ctenopharyngodoni in vitro, method for preparing the medium and method for culture in vitro are provided, which belongs to technical field of in vitro culture of intestinal protozoa. The formulation of the culture medium includes: 100 ml of Ringer's solution, 0.5 g of yeast extract, 1.0 g of proteose peptone, 3-6 ml of fetal bovine serum, 6-10 ml of horse serum, 300-500 μl of Bacillus licheniformis suspension, 200-300 mg of aseptic starch. Culture steps include: inoculating collected Balantidium ctenopharyngodoni into a prepared medium, filling with nitrogen and sealing, culturing at 15° C. for 48-72 hours, then transferring to another fresh medium, cycling back and forth, proliferating the Balantidium ctenopharyngodoni continuously. Balantidium ctenopharyngodoni are capable of achieving cell division and proliferation in the culture medium, and lays foundation for the physiological and experimental ecology research of the Balantidium ctenopharyngodoni.
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公开(公告)号：US20050257281A1
公开(公告)日：2005-11-17
申请号：US11094286
申请日：2005-03-31
申请人： Wei Hu , Zuoyan Zhu , Yaping Wang , Shuangfei Li
发明人： Wei Hu , Zuoyan Zhu , Yaping Wang , Shuangfei Li
IPC分类号： A01K61/00 , A01K67/027 , C07K14/46
CPC分类号： C07K14/461 , A01K67/0275 , A01K2217/05 , A01K2227/40 , A01K2267/02
摘要： The present application disclosed a method for preparing a sterile transgenic fish, comprising constructing antisense RNA expression vector of salmon-type gonadotropin-releasing hormone, introducing the recombinant DNA fragment into carp oosperm by microinjection, and screening the sterile transgenic fish by Polymerase Chain Reaction and radioimmunoassay, wherein the expression vector comprising a promoter of carp beta actin (β-actin) gene, a complementary DNA fragment of antisense salmon-type gonadotropin-releasing hormone (sGnRH) gene from carp with 323 bp as a target gene comprising sGnRH decapeptide, the coding region of gonadotropin-releasing hormone associated peptide and 3′ non-coding sequence, and 3′ flanking sequence of grass carp growth hormone gene as a stop sequence. The method of the present invention is easy and convenient for operation which provides a basis for providing a technical platform of general applicability for solving the hereditary and ecological safety problems of transgenic fishes.
摘要翻译： 本申请公开了一种制备无菌转基因鱼的方法，包括构建鲑鱼型促性腺激素释放激素的反义RNA表达载体，通过显微注射将重组DNA片段引入鲤鱼胚乳，并通过聚合酶链式反应筛选无菌转基因鱼， 放射免疫测定法，其中包含鲤鱼β肌动蛋白（β-肌动蛋白）基因启动子的表达载体，具有323bp作为靶基因的来自鲤鱼的反义鲑鱼型促性腺激素释放激素（sGnRH）基因的互补DNA片段，其包含sGnRH十肽， 促性腺激素释放激素相关肽和3'非编码序列的编码区和草鱼生长激素基因的3'侧翼序列作为停止序列。 本发明的方法操作简单方便，为提供解决转基因鱼遗传和生态安全问题的通用技术平台提供依据。
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公开(公告)号：US10701907B2
公开(公告)日：2020-07-07
申请号：US15870334
申请日：2018-01-12
发明人： Wei Hu , Mouyan Jiang , Yongming Li , Binbin Tao , Ji Chen , Zuoyan Zhu
IPC分类号： A01K67/027 , C12N15/00 , A01K61/17 , C12N15/01 , A61K31/565 , A61D19/02 , A01K61/95 , A01K61/80 , A61K31/167
摘要： Method for producing YY super-male and XY physiological female common carps, with which YY-chromosome super-male common carp can be cultivated and androgenetic YY super-male common carp is produced, where microsatellite markers are used for paternity testing and test crossing confirmation. The YY common carp is crossed with normal female common carp to produce the progenies of only male common carp, and without sex identification, the juvenile male common carp with known sex are subjected to artificial sex reversal to produce XY physiological female common carp.
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公开(公告)号：US20180273598A1
公开(公告)日：2018-09-27
申请号：US15730473
申请日：2017-10-11
发明人： Xi LI , Zhan YIN , Kezhi DAI , Minjie YE , Xuyan PENG , Xianyong ZHOU , Miaomiao ZHENG
IPC分类号： C07K14/575 , G01N33/74 , A61K31/185
CPC分类号： C07K14/575 , A61K31/185 , G01N33/74 , G01N2333/575 , G01N2800/305
摘要： The present invention provides a marker for ADHD and applications thereof. The marker is ghrelin, and the marker can screen for potential ADHD patients by detecting the marker in peripheral blood or cerebrospinal fluid. Further, the present invention provides a new drug for the treatment of ADHD, providing the possibility and hope of new treatment for ADHD patients.
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公开(公告)号：US09187764B2
公开(公告)日：2015-11-17
申请号：US14247145
申请日：2014-04-07
发明人： Wei Hu , Yunsheng Zhang , Jun Dai , Zuoyan Zhu
IPC分类号： C12N15/85 , A01K67/027
CPC分类号： C12N15/8509 , A01K67/0275 , A01K2217/058 , A01K2217/15 , A01K2227/40 , A01K2267/02 , C07K14/461 , C12N2830/002
摘要： The invention discloses a method for controlling fish reproduction, the method comprises the following steps of: A, constructing a recombinant gene CMV-eGFP-SV40-CMV-Ga14-SV40 and establishing a GAL4 transgenic zebrafish line; B, constructing a recombinant gene CMV-RFP-SV40-UAS-antisense dnd and establishing a uas-antisensednd transgenic zebrafish line; and C, hybridizing by taking homozygous transgenic fish of the two lines as parents, and the hybrid generation fish was sterility. The method of the present invention takes a strategy that two fertilizable lines hybridized but hybrid generation is sterility, establishing a novel method with a universal meaning for controlling fish fertility; The method solves the contradiction of the reproductively sterility of fish and the hereditability of characters in a reproduction operation research and can be effectively applied to cultivation of new fish specie and population control.
摘要翻译： 本发明公开了一种鱼繁殖控制方法，该方法包括：构建重组基因CMV-eGFP-SV40-CMV-Ga14-SV40，建立GAL4转基因斑马鱼系; B，构建重组基因CMV-RFP-SV40-UAS反义dnd，建立uas-antensednd转基因斑马鱼系; 和C，通过以两条线的纯合转基因鱼作为亲本杂交，并且杂交代鱼不育。 本发明的方法采取两条可施肥的杂交品系，但是杂交代为不育的策略，建立了一种具有控制鱼类生育能力的普遍意义的新方法; 该方法解决了鱼类繁殖不育与繁殖作业研究中人物遗传性的矛盾，可有效应用于新种鱼种群和群体控制的培育。
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