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9 条记录 (耗时 0.022 秒)

    SCALABLE FERMENTATION PROCESS
    1.
    发明申请
    SCALABLE FERMENTATION PROCESS 有权

    公开(公告)号：US20220089648A1

    公开(公告)日：2022-03-24

    申请号：US17229355

    申请日：2021-04-13

    申请人： KUROS BIOSCIENCES AG

    发明人： Marcel EMMERLING ,  Frank HENNECKE ,  Holger PFRÜNDER ,  Martin RHIEL ,  Philipp STEINER

    IPC分类号： C07K14/005 ,  C12N7/00

    摘要： This invention provides a robust fermentation process for the expression of a capsid protein of a bacteriophage which is forming a VLP by self-assembly, wherein the process is scalable to a commercial production scale and wherein the expression rate of the capsid protein is controlled to obtain improved yield of soluble capsid protein. This is achieved by combining the advantages of fed-batch culture and of lactose induced expression systems with specific process parameters providing improved repression of the promoter during the growth phase and high plasmid retention throughout the process.

    Oligonucleotides packaged into virus-like particles of RNA bacteriophages
    6.
    发明授权
    Oligonucleotides packaged into virus-like particles of RNA bacteriophages 有权

    公开(公告)号：US10358656B2

    公开(公告)日：2019-07-23

    申请号：US15872594

    申请日：2018-01-16

    申请人： Kuros Biosciences AG

    发明人： Matthias Kinzler ,  Karl Proba

    IPC分类号： C12N15/117 ,  C12N7/00 ,  C07H21/04 ,  C12N15/86 ,  A61K39/00

    摘要： The invention provides processes for the producing compositions comprising (i) a virus-like particle, wherein said virus-like particle is a virus-like particle of an RNA bacteriophage, and (ii) an oligonucleotide, wherein said oligonucleotide is packaged into said virus-like particle. The invention further provides processes for producing nucleotide compositions comprising oligonucleotides suitable to be used in the processes mentioned before. The invention further provides nucleotide compositions obtainable by the processes of the invention and uses thereof. The invention further provides compositions comprising (i) a virus-like particle, wherein said virus-like particle is a virus-like particle of an RNA bacteriophage, and (ii) an oligonucleotide, wherein said oligonucleotide is packaged into said virus-like particle, wherein said compositions are obtainable by the processes of the invention and wherein said compositions preferably comprises a purity of at least 98%, most preferably of at least 99%.

    Processes for packaging oligonucleotides into virus-like particles of RNA bacteriophages
    7.
    发明授权
    Processes for packaging oligonucleotides into virus-like particles of RNA bacteriophages 有权

    公开(公告)号：US09902972B2

    公开(公告)日：2018-02-27

    申请号：US13945708

    申请日：2013-07-18

    申请人： Kuros Biosciences AG

    发明人： Matthias Kinzler ,  Karl Proba

    IPC分类号： C12N7/00 ,  C12N15/117 ,  C12N15/86 ,  A61K39/00

    CPC分类号： C12N15/86 ,  A61K2039/5258 ,  C12N7/00 ,  C12N15/117 ,  C12N2310/17 ,  C12N2320/32 ,  C12N2795/00034 ,  C12N2795/18122 ,  C12N2795/18123

    摘要： The invention provides processes for the producing compositions comprising (i) a virus-like particle, wherein said virus-like particle is a virus-like particle of an RNA bacteriophage, and (ii) an oligonucleotide, wherein said oligonucleotide is packaged into said virus-like particle. The invention further provides processes for producing nucleotide compositions comprising oligonucleotides suitable to be used in the processes mentioned before. The invention further provides nucleotide compositions obtainable by the processes of the invention and uses thereof. The invention further provides compositions comprising (i) a virus-like particle, wherein said virus-like particle is a virus-like particle of an RNA bacteriophage, and (ii) an oligonucleotide, wherein said oligonucleotide is packaged into said virus-like particle, wherein said compositions are obtainable by the processes of the invention and wherein said compositions preferably comprises a purity of at least 98%, most preferably of at least 99%.

    Processes for packaging aggregated oligonucleotides into virus-like particles of RNA bacteriophages
    9.
    发明授权
    Processes for packaging aggregated oligonucleotides into virus-like particles of RNA bacteriophages 有权
    将聚合的寡核苷酸包装成RNA噬菌体的病毒样颗粒的方法

    公开(公告)号：US09404126B2

    公开(公告)日：2016-08-02

    申请号：US13942483

    申请日：2013-07-15

    申请人： Cytos Biotechnology AG

    发明人： Matthias Kinzler ,  Karl Proba

    IPC分类号： C12N15/86 ,  C12N7/00 ,  C12N15/117 ,  A61K39/00

    CPC分类号： C12N15/86 ,  A61K2039/5258 ,  C12N7/00 ,  C12N15/117 ,  C12N2310/17 ,  C12N2320/32 ,  C12N2795/00034 ,  C12N2795/18122 ,  C12N2795/18123

    摘要： The invention provides processes for the producing compositions comprising (i) a virus-like particle, wherein said virus-like particle is a virus-like particle of an RNA bacteriophage, and (ii) an oligonucleotide, wherein said oligonucleotide is packaged into said virus-like particle. The invention further provides processes for producing nucleotide compositions comprising oligonucleotides suitable to be used in the processes mentioned before. The invention further provides nucleotide compositions obtainable by the processes of the invention and uses thereof. The invention further provides compositions comprising (i) a virus-like particle, wherein said virus-like particle is a virus-like particle of an RNA bacteriophage, and (ii) an oligonucleotide, wherein said oligonucleotide is packaged into said virus-like particle, wherein said compositions are obtainable by the processes of the invention and wherein said compositions preferably comprises a purity of at least 98%, most preferably of at least 99%.

    摘要翻译： 本发明提供了生产组合物的方法，其包括（i）病毒样颗粒，其中所述病毒样颗粒是RNA噬菌体的病毒样颗粒，和（ii）寡核苷酸，其中所述寡核苷酸被包装入所述病毒 样颗粒。 本发明进一步提供了生产核苷酸组合物的方法，该方法包括适用于上述方法的寡核苷酸。 本发明还提供可通过本发明的方法获得的核苷酸组合物及其用途。 本发明还提供了包含（i）病毒样颗粒的组合物，其中所述病毒样颗粒是RNA噬菌体的病毒样颗粒，和（ii）寡核苷酸，其中所述寡核苷酸包装在所述病毒样颗粒中 其中所述组合物可通过本发明的方法获得，并且其中所述组合物优选包含至少98％，最优选至少99％的纯度。