公开(公告)号：US20230405583A1

公开(公告)日：2023-12-21

申请号：US18029008

申请日：2023-03-28

Applicant: 1087 Systems, Inc.

Inventor： MATTHIAS WAGNER

IPC: B01L3/00 ,  C12M1/34 ,  C12M3/06 ,  C12M1/26

CPC classification number: B01L3/502715 ,  C12M41/30 ,  C12M23/16 ,  C12M33/07 ,  B01L2300/0654 ,  B01L2400/0487 ,  B01L2300/0877

Abstract: Combining through self-modulation a flow of active media from a working vessel of a bioprocess together with a flow of reference media and making a time- and/or spatially-resolved referenced optical measurement of the active vs reference media in a confined flow region and time, such that the two liquids are measured in substantially identical conditions.

公开(公告)号：US11193879B2

公开(公告)日：2021-12-07

申请号：US17221545

申请日：2021-04-02

Applicant: 1087 Systems, Inc.

Inventor： Matthias Wagner ,  John Heanue

IPC: G01J3/44 ,  G01N15/14 ,  G01N29/24 ,  G01N21/17 ,  B07C5/34 ,  G01N21/65 ,  G01N21/3563 ,  B01L3/00 ,  G01N21/35 ,  C12N5/071 ,  G01N15/10 ,  G01N15/00

Abstract: An apparatus for sorting cells includes a measurement volume that contains a cell to be measured, a light source that provides light to cause an emission by a fluorescent label attached to the cell, and an optic device that directs the light through the measurement volume. The apparatus flows the cells through the measurement volume such that as the cell flows through the measurement volume, it interacts with the light, resulting in a change in light originating from the measurement volume, the change in light is a fluorescence emission. Another optic device directs a portion of the light originating from the measurement volume to a detector, which detects the portion of the light. A processor operably coupled to the detector generates an estimate of DNA quantity in the cell based on the change in light originating from the measurement volume, and determines a characteristic of the cell from the estimate.

公开(公告)号：US10677710B2

公开(公告)日：2020-06-09

申请号：US16203901

申请日：2018-11-29

Applicant: 1087 Systems, Inc.

Inventor： Matthias Wagner

IPC: G01N15/14 ,  G01N21/39 ,  G01N21/35 ,  G01N21/3577 ,  G01N21/53 ,  G01N15/06 ,  G01N15/00 ,  G02B21/00 ,  G01N21/3581 ,  G01N21/552 ,  G01N15/10

Abstract: An analyzer of a component in a sample fluid includes an optical source and an optical detector defining a beam path of a beam, wherein the optical source emits the beam and the optical detector measures the beam after partial absorption by the sample fluid, a fluid flow cell disposed on the beam path defining an interrogation region in the a fluid flow cell in which the optical beam interacts with the sample fluid and a reference fluid; and wherein the sample fluid and the reference fluid are in laminar flow, and a scanning system that scans the beam relative to the laminar flow within the fluid flow cell, wherein the scanning system scans the beam relative to both the sample fluid and the reference fluid.

公开(公告)号：US20220196540A1

公开(公告)日：2022-06-23

申请号：US17692876

申请日：2022-03-11

Applicant: 1087 Systems, Inc.

Inventor： Matthias Wagner

IPC: G01N15/14 ,  G01N21/3577 ,  G01N21/39 ,  G01N21/53 ,  G01N15/06 ,  G01N15/00 ,  G01N21/35 ,  G02B21/00

Abstract: An analyzer of a component in a sample fluid includes an optical source and an optical detector defining a beam path of a beam, wherein the optical source emits the beam and the optical detector measures the beam after partial absorption by the sample fluid, a fluid flow cell disposed on the beam path defining an interrogation region in the a fluid flow cell in which the optical beam interacts with the sample fluid and a reference fluid; and wherein the sample fluid and the reference fluid are in laminar flow, and a scanning system that scans the beam relative to the laminar flow within the fluid flow cell, wherein the scanning system scans the beam relative to both the sample fluid and the reference fluid.

公开(公告)号：US10261012B2

公开(公告)日：2019-04-16

申请号：US15174681

申请日：2016-06-06

Applicant: 1087 Systems, Inc.

Inventor： Matthias Wagner

IPC: G01N21/35 ,  G01N21/3577 ,  G01N15/14 ,  G01N21/53 ,  G01N33/487 ,  G01N15/00

Abstract: This disclosure concerns methods and apparatus for interferometric spectroscopic measurements of particles with higher signal to noise ratio utilizing an infrared light beam that is split into two beams. At least one beam may be directed through a measurement volume containing a sample including a medium. The two beams may then be recombined and measured by a detector. The phase differential between the two beams may be selected to provide destructive interference when no particle is present in the measurement volume. A sample including medium with a particle is introduced to the measurement volume and the detected change resulting from at least one of resonant mid-infrared absorption, non-resonant mid-infrared absorption, and scattering by the particle may be used to determine a property of the particle. A wide range of properties of particles may be determined, wherein the particles may include living cells.

公开(公告)号：US10175159B2

公开(公告)日：2019-01-08

申请号：US15226899

申请日：2016-08-02

Applicant: 1087 Systems, Inc.

Inventor： Matthias Wagner ,  John Heanue

IPC: B01L3/00 ,  B07C5/34 ,  C12N5/071 ,  G01N15/00 ,  G01N15/10 ,  G01N21/17 ,  G01N29/24 ,  G01N21/3563 ,  G01N15/14

Abstract: This disclosure concerns a cytometry system including a handling system that enables presentation of single cells to at least one laser source. The laser source is configured to deliver light to a cell within the cells in order to induce bond vibrations in the cellular DNA. The system further includes a detection facility that detects the signature of the bond vibrations, wherein the bond vibration signature is used to determine the folding or packing of the DNA.

公开(公告)号：US09410883B2

公开(公告)日：2016-08-09

申请号：US13894831

申请日：2013-05-15

Applicant: 1087 Systems, Inc.

Inventor： Matthias Wagner

IPC: G01J5/20 ,  G01N21/53 ,  G01N15/14

Abstract: This disclosure concerns methods and apparatus for interferometric spectroscopic measurements of particles with higher signal to noise ratio utilizing an infrared light beam that is split into two beams. At least one beam may be directed through a measurement volume containing a sample including a medium. The two beams may then be recombined and measured by a detector. The phase differential between the two beams may be selected to provide destructive interference when no particle is present in the measurement volume. A sample including medium with a particle is introduced to the measurement volume and the detected change resulting from at least one of resonant mid-infrared absorption, non-resonant mid-infrared absorption, and scattering by the particle may be used to determine a property of the particle. A wide range of properties of particles may be determined, wherein the particles may include living cells.

公开(公告)号：US11965816B2

公开(公告)日：2024-04-23

申请号：US17164710

申请日：2021-02-01

Applicant: 1087 Systems, Inc.

Inventor： Matthias Wagner ,  John Heanue

IPC: G01N15/14 ,  B01L3/00 ,  B07C5/34 ,  C12N5/071 ,  G01N15/00 ,  G01N15/10 ,  G01N15/1429 ,  G01N15/1434 ,  G01N21/17 ,  G01N21/35 ,  G01N21/3563 ,  G01N21/65 ,  G01N29/24

CPC classification number: G01N15/147 ,  B01L3/502761 ,  B07C5/34 ,  C12N5/0612 ,  G01N15/14 ,  G01N15/1427 ,  G01N15/1429 ,  G01N15/1434 ,  G01N15/1436 ,  G01N15/1459 ,  G01N15/1475 ,  G01N15/1484 ,  G01N21/1702 ,  G01N21/35 ,  G01N21/3563 ,  G01N21/65 ,  G01N29/2418 ,  B01L2200/0636 ,  B01L2200/0652 ,  B01L2300/0627 ,  B01L2300/0864 ,  G01N2015/0065 ,  G01N2015/1006 ,  G01N15/1463 ,  G01N2015/1481 ,  G01N2015/149 ,  G01N2015/1493 ,  G01N2015/1497 ,  G01N2021/653 ,  G01N2201/0612

Abstract: This disclosure concerns a cytometry system including a handling system that enables presentation of single cells to at least one laser source. The laser source is configured to deliver light to a cell within the cells in order to induce bond vibrations in the cellular DNA. The system further includes a detection facility that detects the signature of the bond vibrations, wherein the bond vibration signature is used to determine the folding or packing of the DNA.

公开(公告)号：US20240060031A1

公开(公告)日：2024-02-22

申请号：US18028997

申请日：2021-09-29

Applicant: 1087 Systems, Inc

Inventor： MATTHIAS WAGNER

IPC: C12M1/36 ,  C12M1/00 ,  C12M1/34 ,  G01N21/85

CPC classification number: C12M41/48 ,  C12M29/10 ,  C12M41/30 ,  C12M41/38 ,  C12M41/36 ,  C12M47/10 ,  G01N21/85 ,  G01N2021/8557 ,  G01N2021/8411

Abstract: Combining through self-modulation a flow of active media from a working vessel of a bioprocess together with a flow of reference media and making a time- and/or spatially-resolved referenced optical measurement of the active vs reference media in a confined flow region and time, such that the two liquids are measured in substantially identical conditions.

公开(公告)号：US20210223159A1

公开(公告)日：2021-07-22

申请号：US17221545

申请日：2021-04-02

Applicant: 1087 Systems, Inc.

Inventor： Matthias Wagner ,  John Heanue

IPC: G01N15/14 ,  G01N21/3563 ,  G01N21/17 ,  G01N21/35 ,  G01N29/24 ,  C12N5/071 ,  G01N21/65 ,  B01L3/00 ,  B07C5/34

Abstract: An apparatus for sorting cells includes a measurement volume that contains a cell to be measured, a light source that provides light to cause an emission by a fluorescent label attached to the cell, and an optic device that directs the light through the measurement volume. The apparatus flows the cells through the measurement volume such that as the cell flows through the measurement volume, it interacts with the light, resulting in a change in light originating from the measurement volume, the change in light is a fluorescence emission. Another optic device directs a portion of the light originating from the measurement volume to a detector, which detects the portion of the light. A processor operably coupled to the detector generates an estimate of DNA quantity in the cell based on the change in light originating from the measurement volume, and determines a characteristic of the cell from the estimate.