公开(公告)号：US08363223B2

公开(公告)日：2013-01-29

申请号：US13348663

申请日：2012-01-12

申请人： Boaz Ran ,  Ariel G. Notcovich ,  Ariel Lipson ,  Shay Nimri ,  Stephen G. Lipson ,  Doron Lipson

发明人： Boaz Ran ,  Ariel G. Notcovich ,  Ariel Lipson ,  Shay Nimri ,  Stephen G. Lipson ,  Doron Lipson

IPC分类号： G01N21/55

CPC分类号： G01N21/55 ,  G01N21/553 ,  G01N2021/058

摘要： An SPR sensor comprising a thin conducting layer comprising at least one conductive element formed on a surface of a transparent substrate, a light source that illuminates an interface between the conducting layer and the substrate, a photosensitive surface that generates signals from light reflected from the interface, a flow cell formed with at least one flow channel having a lumen defined by a wall formed from an elastic material and from a region of the conducting layer, and at least one hollow fluid-providing flow control apparatus having a lumen and an orifice communicating with its lumen. Fluid flow is enabled between the flow channel and the lumen of the flow control apparatus by forcing an end of the flow control apparatus through the elastic material so that the orifice communicates with the flow channel lumen.

摘要翻译： 一种SPR传感器，包括薄导电层，该导电层包括形成在透明基板的表面上的至少一个导电元件，照亮导电层和基板之间的界面的光源，从该界面反射的光产生信号的光敏表面 ，形成有至少一个流动通道的流动池，所述流动通道具有由由弹性材料形成的壁和从导电层的区域限定的内腔，以及至少一个中空流体供应流量控制装置，其具有内腔和孔口连通 与其流明。 通过迫使流量控制装置的一端通过弹性材料使得流体通道与流动控制装置的内腔之间能够进行流体流动，使得孔口与流动通道内腔连通。

公开(公告)号：US08105845B2

公开(公告)日：2012-01-31

申请号：US10578860

申请日：2004-11-14

申请人： Ariel Notcovich ,  Alon Herschhorn ,  Shai Nimri ,  John Barich ,  Ariel Lipson ,  Ran Boaz ,  Doron Lipson ,  Yaakov Levie

发明人： Ariel Notcovich ,  Alon Herschhorn ,  Shai Nimri ,  John Barich ,  Ariel Lipson ,  Ran Boaz ,  Doron Lipson ,  Yaakov Levie

IPC分类号： G01N33/543

CPC分类号： C40B30/04 ,  B01J2219/00657 ,  B01J2219/00659 ,  B01L3/5025 ,  B01L3/5027 ,  B01L3/502715 ,  B01L2300/0636 ,  B01L2300/0877 ,  G01N21/05 ,  G01N21/553 ,  G01N21/648 ,  G01N33/54373 ,  G01N33/5438 ,  G01N33/557 ,  G01N2021/0346 ,  G01N2021/212 ,  G01N2021/6439 ,  G01N2021/6463 ,  G01N2035/00158

摘要： A method for determining one or more kinetic parameters of binding between a first binding member and a second binding member. The method comprises adsorbing the first binding member to a surface at a plurality of microspots. The second binding member is then presented to the first binding member at each of the microspots, there being a plurality of combinations of first binding member surface density and second binding member concentration among the plurality of microspots. Data indicative of a binding reaction between the first of microspots are then obtained and analyzed so as to obtain one or more kinetic parameters of the binding between the first and second binding members. The invention also provides a system for carrying out the method. A method for localizing a molecular species at microspots on a surface, and a probe array produced by the method are also provided.

摘要翻译： 一种用于确定第一结合构件和第二结合构件之间的结合的一个或多个动力学参数的方法。 该方法包括将第一结合构件吸附到多个微点处的表面。 然后将第二结合构件提供给每个微点处的第一结合构件，在多个微孔中存在多个第一结合构件表面密度和第二结合成员浓度的组合。 然后获得并分析指示第一个微孔之间的结合反应的数据，以便获得第一和第二结合成员之间的结合的一个或多个动力学参数。 本发明还提供一种用于执行该方法的系统。 还提供了一种用于定位表面上的微孔分子种类的方法，以及通过该方法制备的探针阵列。

公开(公告)号：US07586616B2

公开(公告)日：2009-09-08

申请号：US12149158

申请日：2008-04-28

申请人： Boaz Ran ,  Ariel G. Notcovich ,  Ariel Lipson ,  Shay Nimri ,  Stephen G. Lipson ,  Doron Lipson

发明人： Boaz Ran ,  Ariel G. Notcovich ,  Ariel Lipson ,  Shay Nimri ,  Stephen G. Lipson ,  Doron Lipson

IPC分类号： G01N21/55

CPC分类号： G01N21/55 ,  G01N21/553 ,  G01N2021/058

摘要： An SPR sensor comprising: a thin conducting layer comprising at least one conductive element formed on a surface of a transparent substrate; an illumination system controllable to illuminate an interface between the conducting layer and the substrate; a photosensitive surface that generates signals responsive to light from the light source that is reflected from a region of the interface; a flow cell formed with at least one flow channel having a lumen defined by a wall at least a portion of which is formed from an elastic material and a portion of which is formed by a region of the conducting layer; and at least one hollow needle having an exit orifice communicating with the needle's lumen and wherein fluid flow is enabled between the flow channel and the needle's lumen by puncturing the elastic material with the at least one needle so that the exit orifice communicates with the flow channel lumen.

摘要翻译： 一种SPR传感器，包括：薄导电层，包括形成在透明基板的表面上的至少一个导电元件; 照明系统，其可控制以照亮所述导电层和所述衬底之间的界面; 感光表面，其产生响应于来自所述界面的区域反射的来自所述光源的光的信号; 形成有至少一个流动通道的流动池，所述流动通道具有由壁限定的内腔，壁的至少一部分由弹性材料形成，并且其一部分由导电层的区域形成; 以及至少一个中空针，其具有与所述针的内腔连通的出口孔，并且通过用所述至少一个针刺穿所述弹性材料，使得所述流动通道与所述针的内腔之间能够流动，使得所述出口与所述流动通道连通 流明。

公开(公告)号：US20060072113A1

公开(公告)日：2006-04-06

申请号：US10540940

申请日：2002-12-25

申请人： Boaz Ran ,  Ariel Notcovich ,  Ariel Lipson ,  Shay Nimri ,  Stephan Lipson ,  Doron Lipson

发明人： Boaz Ran ,  Ariel Notcovich ,  Ariel Lipson ,  Shay Nimri ,  Stephan Lipson ,  Doron Lipson

IPC分类号： G01N21/55

CPC分类号： G01N21/55 ,  G01N21/553 ,  G01N2021/058

摘要： An SPR sensor comprising: a thin conducting layer comprising at least one conductive element formed on a surface of a transparent substrate; an illumination system controllable to illuminate an interface between the conducting layer and the substrate; a photosensitive surface that generates signals responsive to light from the light source that is reflected from a region of the interface; a flow cell formed with at least one flow channel having a lumen defined by a wall at least a portion of which is formed from an elastic material and a portion of which is formed by a region of the conducting layer; and at least one hollow needle having an exit orifice communicating with the needle's lumen and wherein fluid flow is enabled between the flow channel and the needle's lumen by puncturing the elastic material with the at least one needle so that the exit orifice communicates with the flow channel lumen.

公开(公告)号：US08958071B2

公开(公告)日：2015-02-17

申请号：US13509699

申请日：2011-11-15

申请人： Mordehai Margalit ,  Ariel Lipson

发明人： Mordehai Margalit ,  Ariel Lipson

IPC分类号： G01N21/00 ,  G01N21/31 ,  G01N21/65

CPC分类号： G01N21/31 ,  G01N21/65

摘要： Optical sensors for use in examining and analyzing a fluid material are described. The optical sensors include a channel configured to transport a fluid material to be examined and analyzed, a first waveguide positioned adjacent to the channel and configured to guide radiation through the channel and a first tunable grating defined on the first waveguide and configured to variably define a cavity for selecting a first wavelength of the radiation.

摘要翻译： 描述了用于检查和分析流体材料的光学传感器。 光学传感器包括被配置为输送待检查和分析的流体材料的通道，邻近通道定位并被配置为引导辐射通过通道的第一波导和限定在第一波导上的第一可调谐光栅，并且被配置为可变地限定 用于选择辐射的第一波长的腔。

公开(公告)号：US08665434B2

公开(公告)日：2014-03-04

申请号：US13640360

申请日：2012-06-04

申请人： Mordehai Margalit ,  Ariel Lipson

发明人： Mordehai Margalit ,  Ariel Lipson

IPC分类号： G01J3/44

CPC分类号： G01J3/44 ,  G01J3/433 ,  G01J3/453

摘要： A system and method for performing Raman spectroscopy using a heterodyne detection scheme are described. The heterodyne detection scheme can combine a modulated portion of radiation having passed through a sample to be analyzed with a reference radiation to produce a combined frequency signal. At least a portion of the reference radiation can be filtered out of the combined frequency signal, resulting in a filtered frequency signal. Information related to the sample can be determined based upon the filtered signal. The determined information can then be analyzed to determine a chemical composition of the sample.

摘要翻译： 描述使用外差检测方案进行拉曼光谱的系统和方法。 外差检测方案可以将已经通过要分析的样本的经辐射的调制部分与参考辐射组合以产生组合频率信号。 参考辐射的至少一部分可以从组合频率信号中滤出，产生滤波后的频率信号。 可以基于滤波信号来确定与样本有关的信息。 然后可以分析确定的信息以确定样品的化学组成。

公开(公告)号：US20110267622A1

公开(公告)日：2011-11-03

申请号：US13181547

申请日：2011-07-13

申请人： Boaz RAN ,  Ariel G. Notcovich ,  Ariel Lipson ,  Shay Nimri ,  Stephen G. Lipson ,  Doron Lipson

发明人： Boaz RAN ,  Ariel G. Notcovich ,  Ariel Lipson ,  Shay Nimri ,  Stephen G. Lipson ,  Doron Lipson

IPC分类号： G01N21/55

CPC分类号： G01N21/55 ,  G01N21/553 ,  G01N2021/058

摘要： An SPR sensor comprising a thin conducting layer comprising at least one conductive element formed on a surface of a transparent substrate, a light source that illuminates an interface between the conducting layer and the substrate, a photosensitive surface that generates signals from light reflected from the interface, a flow cell formed with at least one flow channel having a lumen defined by a wall formed from an elastic material and from a region of the conducting layer, and at least one hollow fluid-providing flow control apparatus having a lumen and an orifice communicating with its lumen. Fluid flow is enabled between the flow channel and the lumen of the flow control apparatus by forcing an end of the flow control apparatus through the elastic material so that the orifice communicates with the flow channel lumen.

摘要翻译： 一种SPR传感器，包括薄导电层，该导电层包括形成在透明基板的表面上的至少一个导电元件，照亮导电层和基板之间的界面的光源，从该界面反射的光产生信号的光敏表面 ，形成有至少一个流动通道的流动池，所述流动通道具有由由弹性材料形成的壁和从导电层的区域限定的内腔，以及至少一个中空流体供应流量控制装置，其具有内腔和孔口连通 与其流明。 通过迫使流量控制装置的一端通过弹性材料使得流体通道与流动控制装置的内腔之间能够进行流体流动，使得孔口与流动通道内腔连通。

公开(公告)号：US09175421B2

公开(公告)日：2015-11-03

申请号：US13332411

申请日：2011-12-21

申请人： Ariel Notcovich ,  Alon Herschhorn ,  Shai Nimri ,  John Barich ,  Ariel Lipson ,  Ran Boaz ,  Doron Lipson ,  Yaakov Levie

发明人： Ariel Notcovich ,  Alon Herschhorn ,  Shai Nimri ,  John Barich ,  Ariel Lipson ,  Ran Boaz ,  Doron Lipson ,  Yaakov Levie

IPC分类号： C40B30/04 ,  G01N21/552 ,  G01N21/64 ,  G01N33/543 ,  G01N33/557 ,  B01L3/00 ,  G01N21/05 ,  G01N21/21 ,  G01N35/00 ,  G01N21/03

CPC分类号： C40B30/04 ,  B01J2219/00657 ,  B01J2219/00659 ,  B01L3/5025 ,  B01L3/5027 ,  B01L3/502715 ,  B01L2300/0636 ,  B01L2300/0877 ,  G01N21/05 ,  G01N21/553 ,  G01N21/648 ,  G01N33/54373 ,  G01N33/5438 ,  G01N33/557 ,  G01N2021/0346 ,  G01N2021/212 ,  G01N2021/6439 ,  G01N2021/6463 ,  G01N2035/00158

摘要： Provided is a method for determining one or more kinetic parameters of binding between a first binding member and a second binding member. The method includes adsorbing the first binding member to a surface at a plurality of microspots. The second binding member is then presented to the first binding member at each of the microspots, there being a plurality of combinations of first binding member surface density and second binding member concentration among the plurality of microspots. Data indicative of a binding reaction between the first of microspots are then obtained and analyzed so as to obtain one or more kinetic parameters of the binding between the first and second binding members. Also provided is a system for carrying out the method. A method for localizing a molecular species at microspots on a surface, and a probe array produced by the method are also provided.

摘要翻译： 提供了一种用于确定第一结合构件和第二结合构件之间的结合的一个或多个动力学参数的方法。 该方法包括将第一结合构件吸附到多个微点的表面。 然后将第二结合构件提供给每个微点处的第一结合构件，在多个微孔中存在多个第一结合构件表面密度和第二结合成员浓度的组合。 然后获得并分析指示第一个微孔之间的结合反应的数据，以便获得第一和第二结合成员之间的结合的一个或多个动力学参数。 还提供了一种用于执行该方法的系统。 还提供了一种用于定位表面上的微孔分子种类的方法，以及通过该方法制备的探针阵列。

公开(公告)号：US20120129723A1

公开(公告)日：2012-05-24

申请号：US13332411

申请日：2011-12-21

申请人： Ariel NOTCOVICH ,  Alon HERSCHHORN ,  Shai NIMRI ,  John BARICH ,  Ariel LIPSON ,  Ran BOAZ ,  Doron LIPSON ,  Yaakov LEVIE

发明人： Ariel NOTCOVICH ,  Alon HERSCHHORN ,  Shai NIMRI ,  John BARICH ,  Ariel LIPSON ,  Ran BOAZ ,  Doron LIPSON ,  Yaakov LEVIE

IPC分类号： C40B30/04 ,  C40B60/08

CPC分类号： C40B30/04 ,  B01J2219/00657 ,  B01J2219/00659 ,  B01L3/5025 ,  B01L3/5027 ,  B01L3/502715 ,  B01L2300/0636 ,  B01L2300/0877 ,  G01N21/05 ,  G01N21/553 ,  G01N21/648 ,  G01N33/54373 ,  G01N33/5438 ,  G01N33/557 ,  G01N2021/0346 ,  G01N2021/212 ,  G01N2021/6439 ,  G01N2021/6463 ,  G01N2035/00158

摘要： Provided is a method for determining one or more kinetic parameters of binding between a first binding member and a second binding member. The method includes adsorbing the first binding member to a surface at a plurality of microspots. The second binding member is then presented to the first binding member at each of the microspots, there being a plurality of combinations of first binding member surface density and second binding member concentration among the plurality of microspots. Data indicative of a binding reaction between the first of microspots are then obtained and analyzed so as to obtain one or more kinetic parameters of the binding between the first and second binding members. Also provided is a system for carrying out the method. A method for localizing a molecular species at microspots on a surface, and a probe array produced by the method are also provided.

摘要翻译： 提供了一种用于确定第一结合构件和第二结合构件之间的结合的一个或多个动力学参数的方法。 该方法包括将第一结合构件吸附到多个微点的表面。 然后将第二结合构件提供给每个微点处的第一结合构件，在多个微孔中存在多个第一结合构件表面密度和第二结合成员浓度的组合。 然后获得并分析指示第一个微孔之间的结合反应的数据，以便获得第一和第二结合成员之间的结合的一个或多个动力学参数。 还提供了一种用于执行该方法的系统。 还提供了一种用于定位表面上的微孔分子种类的方法，以及通过该方法制备的探针阵列。

公开(公告)号：US20090296095A1

公开(公告)日：2009-12-03

申请号：US12535707

申请日：2009-08-05

申请人： Boaz RAN ,  Ariel G. Notcovich ,  Ariel Lipson ,  Shay Nimri ,  Stephen G. Lipson ,  Doron Lipson

发明人： Boaz RAN ,  Ariel G. Notcovich ,  Ariel Lipson ,  Shay Nimri ,  Stephen G. Lipson ,  Doron Lipson

IPC分类号： G01N21/55 ,  G01N21/01

CPC分类号： G01N21/55 ,  G01N21/553 ,  G01N2021/058

摘要： An SPR sensor comprising a thin conducting layer comprising at least one conductive element formed on a surface of a transparent substrate, a light source that illuminates an interface between the conducting layer and the substrate, a photosensitive surface that generates signals from light reflected from the interface, a flow cell formed with at least one flow channel having a lumen defined by a wall formed from an elastic material and from a region of the conducting layer, and at least one hollow fluid-providing flow control apparatus having a lumen and an orifice communicating with its lumen. Fluid flow is enabled between the flow channel and the lumen of the flow control apparatus by forcing an end of the flow control apparatus through the elastic material so that the orifice communicates with the flow channel lumen.

摘要翻译： 一种SPR传感器，包括薄导电层，该导电层包括形成在透明基板的表面上的至少一个导电元件，照亮导电层和基板之间的界面的光源，从该界面反射的光产生信号的光敏表面 ，形成有至少一个流动通道的流动池，所述流动通道具有由由弹性材料形成的壁和从导电层的区域限定的内腔，以及至少一个中空流体供应流量控制装置，其具有内腔和孔口连通 与其流明。 通过迫使流量控制装置的一端通过弹性材料使得流体通道与流动控制装置的内腔之间能够进行流体流动，使得孔口与流动通道内腔连通。