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13 条记录 (耗时 0.015 秒)

    Labeling reagents, methods for synthesizing such reagents and methods for detecting biological molecules
    3.
    发明申请
    Labeling reagents, methods for synthesizing such reagents and methods for detecting biological molecules 有权
    标记试剂，合成这些试剂的方法和检测生物分子的方法

    公开(公告)号：US20030186448A1

    公开(公告)日：2003-10-02

    申请号：US10137454

    申请日：2002-05-03

    申请人： BIO MERIEUX

    发明人： Cecile Bourget ,  Jean Lhomme ,  Ali Laayoun ,  Mitsuharu Kotera ,  Emmanuelle Trevisiol ,  Lionel Menou ,  Eloy Bernal Mendez

    IPC分类号： G01N033/52

    CPC分类号： C07D209/08 ,  C07C245/14 ,  C07C245/18 ,  C07D311/90 ,  C07D495/04 ,  Y10T436/13 ,  Y10T436/143333

    摘要： The present invention relates to a temperature-stable labeling reagent of formula: 1 in which R1 represents H or an alkyl, aryl or substituted aryl group, R2 represents a detectable marker or at least two detectable markers linked together by at least one multimeric structure, L is a linking arm containing a linear succession of at least two covalent bonds and n an integer equal to 0 or 1, R3 and R4 represent independently of each other: H, NO2, Cl, Br, F, I, R2-(L)n-YnullXnull, OR, SR, NR2, R, NHCOR, CONHR, COOR with Rnullalkyl or aryl, A is a linking arm containing at least one covalent double bond allowing conjugation of the diazo functional group with the aromatic ring and u is an integer between 0 and 2, preferably equal to 0 or 1, and nullYnullXnull represents nullCONHnull, nullNHCOnull, nullCH2Onull, nullCH2Snull. The present invention also describes a method for synthesizing said markers as well as applications for labeling biological molecules, in particular nucleic acids, with a labeling reagent carrying the diazomethyl functional group. The invention finds a preferred application in the field of diagnosis.

    摘要翻译： 本发明涉及下式的温度稳定的标记试剂：其中R 1表示H或烷基，芳基或取代的芳基，R 2表示可检测标记或至少两个可检测标记通过在 至少一个多聚体结构，L是包含至少两个共价键的线性连续的连接臂，n是等于0或1的整数，R 3和R 4彼此独立地表示：H，NO 2，Cl ，Br，F，I，R 2 - （L）nYX-，OR，SR，NR 2，R，NHCOR，CONHR，COOR，其中R =烷基或芳基，A是含有至少一个共价双键的连接臂 允许重氮官能团与芳族环共轭，u是0和2之间的整数，优选等于0或1，-YX-表示-CONH-，-NHCO - ， - CH 2 O-， - CH 2 S-。 本发明还描述了一种用于合成所述标记的方法以及用携带重氮甲基官能团的标记试剂标记生物分子，特别是核酸的应用。 本发明在诊断领域找到优选的应用。

    Method for in vitro culture of viruses of the togaviridae and flaviviridae families and uses
    4.
    发明申请
    Method for in vitro culture of viruses of the togaviridae and flaviviridae families and uses 审中-公开
    披膜病毒和黄病毒科的病毒体外培养方法及用途

    公开(公告)号：US20040229336A1

    公开(公告)日：2004-11-18

    申请号：US10776617

    申请日：2004-02-12

    申请人： Bio Merieux ,  Institut National De La Sante Et De La Recherche Medicale

    发明人： Patrice Andre ,  Vincent Lotteau ,  Glaucia Paranhos-Baccala ,  Florence Komurian-Pradel

    IPC分类号： C12N007/00

    CPC分类号： C12N7/00 ,  C12N2770/24051 ,  G01N33/56983 ,  G01N2333/18 ,  Y02A50/60

    摘要： The invention concerns a method of culturing, propagating and replicating in vitro viruses of the Togaviridae and Flaviviridae families which consists in: providing at least a fraction of LVP's obtained from serum or plasma of a patient infected with a least a virus of the Togaviridae and Flaviviridae families; contacting said fraction, for a predetermined time interval in an appropriate culture medium, with permissive cells having an endocytosis path relayed by at least one receptor of the lipoproteins and modulated inter alia by an activating agent selected among an unsaturated fatty acid or an unsaturated fatty acid derivative comprising 16 to 20 carbon atoms or their mixture. The invention also concerns the uses of viral particles and polypeptides obtained by said method.

    摘要翻译： 本发明涉及一种培养，繁殖和复制沙眼病毒科和黄病毒科的体外病毒的方法，该方法包括：提供至少一部分血清或血浆中获得的至少一种感染了披属病毒科和黄病毒科的病毒的患者的LVP 家庭; 在合适的培养基中使所述级分接触预定的时间间隔，其中容许细胞具有由至少一种脂蛋白受体中和的内吞途径，并特别通过选自不饱和脂肪酸或不饱和脂肪酸的活化剂调节 包含16-20个碳原子的衍生物或它们的混合物。 本发明还涉及通过所述方法获得的病毒颗粒和多肽的用途。

    The LTR region of MSRV-1 and the proteins it encodes and probes and methods for detecting MSRV-1 retrovirus
    5.
    发明申请
    The LTR region of MSRV-1 and the proteins it encodes and probes and methods for detecting MSRV-1 retrovirus 失效
    MSRV-1的LTR区及其编码的蛋白质，探针和检测MSRV-1逆转录病毒的方法

    公开(公告)号：US20040043381A1

    公开(公告)日：2004-03-04

    申请号：US10637565

    申请日：2003-08-11

    申请人： Bio Merieux

    发明人： Glaucia Paranhos-Baccala ,  Herve Perron ,  Florence Komurian-Pradel

    IPC分类号： C12Q001/70 ,  C07H021/04 ,  C12N007/00 ,  C07K014/15

    CPC分类号： C07K14/005 ,  C12N2740/10022

    摘要： The invention relates to a nucleotide fragment of a LTR-RU5 region comprising a nucleotide sequence which encodes the expression of a protein, wherein said protein comprises a peptide sequence selected from SEQ ID NO:2, SEQ ID NO:3 and SEQ ID NO:4, and a complementary nucleotide fragment; a probe and a primer hybridizing with said fragment; a protein encoded by said fragment; an antibody directed against said protein; and a process for detecting the presence of MSRV-1 retrovirus using a probe or an antibody of the invention.

    摘要翻译： 本发明涉及包含编码蛋白质表达的核苷酸序列的LTR-RU5区域的核苷酸片段，其中所述蛋白质包含选自SEQ ID NO：2，SEQ ID NO：3和SEQ ID NO： 4和互补核苷酸片段; 探针和与所述片段杂交的引物; 由所述片段编码的蛋白质; 针对所述蛋白质的抗体; 以及使用本发明的探针或抗体检测MSRV-1逆转录病毒的存在的方法。

    Process for labeling a ribonucleic acid
    8.
    发明申请
    Process for labeling a ribonucleic acid 失效
    用于标记核糖核酸的方法

    公开(公告)号：US20020187500A1

    公开(公告)日：2002-12-12

    申请号：US10101362

    申请日：2002-03-20

    申请人： Bio Merieux

    发明人： Ali Laayoun

    IPC分类号： C12Q001/68 ,  C07H021/02 ,  C12P019/34

    CPC分类号： C07H21/00

    摘要： The present invention relates to a process for labeling a synthetic or natural ribonucleic acid (RNA). It also relates to RNA fragments, which have been labeled by fragmenting the RNA to free a terminal phosphate of each fragment for further reaction, and labeling each fragment at the freed terminal phosphate which is located at the 3null end and/or the 5null end of each fragment of the RNA, and to the use of such RNA fragments, for example, in the field of medical diagnosis.

    摘要翻译： 本发明涉及用于标记合成或天然核糖核酸（RNA）的方法。 它还涉及RNA片段，其已经通过片段化RNA来标记以释放每个片段的末端磷酸酯用于进一步的反应，并且在位于3'末端和/或5'端的释放的磷酸末端标记每个片段， RNA的每个片段的末端，以及例如在医学诊断领域中使用这样的RNA片段。