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18 条记录 (耗时 0.016 秒)

    Large scale method for purification of high purity heparinase from flavobacterium heparinum
    3.
    发明授权
    Large scale method for purification of high purity heparinase from flavobacterium heparinum 失效
    从肝素肝素纯化高纯度肝素酶的大规模方法

    公开(公告)号：US5169772A

    公开(公告)日：1992-12-08

    申请号：US726646

    申请日：1991-07-02

    申请人： Joseph J. Zimmerman ,  Charles L. Cooney

    发明人： Joseph J. Zimmerman ,  Charles L. Cooney

    IPC分类号： C07K16/40 ,  C12N9/88 ,  C12N15/00

    CPC分类号： C07K16/40 ,  C12N15/00 ,  C12N9/88 ,  Y10S435/85

    摘要： The present invention is an improved process for purification of active heparinase and heparinase like enzymes from Gram negative organisms, in particular, Flavobacterium heparinum. The primary advantage of the process is the fact that it allows large scale processing and high yield of heparinase. The heparinase is released from the periplasmic space of the organism by osmotic shock treatment, first into an osmotically stabilized medium, secondly into a non-stabilized medium having a pH of approximately pH 6.0 and 8.6 with subsequent release into a second non-stabilized medium containing approximately 0.15 M sodium chloride, followed by fractionation by cation exchange chromatography, and, optionally, electropheresis or gel filtration chromatography. Two proteins having heparinase activity have been isolated, one having a molecular weight of approximately 42,000 Daltons and the other having a molecular weight of 65,000 to 75,000 Daltons.Also described is the construction of a library for screening for the genes encoding the proteins having heparinase activity and two assay for detecting organisms producing heparinase, either F. heparinum or genetically engineered organisms.

    摘要翻译： 本发明是用于从革兰氏阴性生物体，特别是肝炎黄杆菌纯化活性肝素酶和肝素酶样酶的改进方法。 该方法的主要优点是其允许大规模加工和高产量的肝素酶。 肝素酶通过渗透冲击处理从生物体的周质空间释放，首先进入渗透稳定的培养基，其次转化为pH约为6.0和8.6的非稳定培养基，随后释放到含有 约0.15M氯化钠，然后通过阳离子交换层析分离，并且任选地进行电穿孔或凝胶过滤层析。 已经分离出两种具有肝素酶活性的蛋白质，分子量约为42,000道尔顿，另一种具有65,000至75,000道尔顿分子量。 还描述了用于筛选编码具有肝素酶活性的蛋白质的基因的文库的构建和用于检测产生肝素酶（肝素肝素或基因工程生物体）的生物体的两种测定。

    L-glutamine sensor
    4.
    发明授权
    L-glutamine sensor 失效
    L-谷氨酰胺传感器

    公开(公告)号：US4780191A

    公开(公告)日：1988-10-25

    申请号：US67641

    申请日：1987-06-26

    申请人： Jean L. Romette ,  Charles L. Cooney

    发明人： Jean L. Romette ,  Charles L. Cooney

    IPC分类号： G01N27/327 ,  C12Q1/00 ,  G01N27/30

    CPC分类号： C12Q1/005 ,  Y10S435/817

    摘要： Apparatus for measuring L-glutamine in a liquid sample includes a membrane on which are immobilized the enzymes glutaminase and glutamate oxidase, whereby any said L-glutamine in the sample is acted upon by the glutaminase to form glutamic acid, the glutamic acid being acted upon by the glutamate oxidase to form an enzymatic reaction product, the membrane being operatively associated with a sensor capable of sensing the enzymatic reaction product or a compound or element consumed or liberated in the formation or degradation thereof.

    摘要翻译： 用于测量液体样品中的L-谷氨酰胺的装置包括其上固定有酶谷氨酰胺酶和谷氨酸氧化酶的膜，由此样品中的任何所述L-谷氨酰胺被谷氨酰胺酶作用以形成谷氨酸，谷氨酸被作用于 通过谷氨酸氧化酶形成酶反应产物，该膜与能够感测酶反应产物或在其形成或降解中消耗或释放的化合物或元素的传感器可操作地相关联。

    Process for producing maltase
    6.
    发明授权
    Process for producing maltase 失效
    麦芽糖酶的制备方法

    公开(公告)号：US4332899A

    公开(公告)日：1982-06-01

    申请号：US177709

    申请日：1980-08-13

    申请人： Charles L. Cooney ,  Eugene J. Schaefer

    发明人： Charles L. Cooney ,  Eugene J. Schaefer

    IPC分类号： C12N9/26 ,  C12N15/00

    CPC分类号： C12N9/2408 ,  C12R1/85 ,  Y10S435/94

    摘要： Maltase is produced by growing a mutant of the yeast strain Saccharomyces italicus capable of growing in a growth medium utilizing sucrose as the carbon source. Mutants of Saccharomyces italicus capable of utilizing sucrose as a carbon source also are provided.

    摘要翻译： 通过使用蔗糖作为碳源，生长能够在生长培养基中生长的酵母菌丝状酵母菌（Saccharomyces italicus）的突变体来产生麦芽糖酶。 还提供了能够利用蔗糖作为碳源的酿酒酵母的突变体。