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8 条记录 (耗时 0.02 秒)

    Capillary array-based sample screening
    1.
    发明申请
    Capillary array-based sample screening 审中-公开

    公开(公告)号：US20030013115A1

    公开(公告)日：2003-01-16

    申请号：US10237246

    申请日：2002-09-04

    申请人： Diversa Corporation, a Delaware corporation

    发明人： William Michael Lafferty

    IPC分类号： C12Q001/68 ,  C12M001/34

    CPC分类号： G01N21/0303 ,  G01N21/253

    摘要： A sample screening platform, system and method for screening and recovery of detectable samples. The platform includes a capillary array including a plurality of capillaries. Each capillary includes at least one wall defining a lumen for retaining the detectable sample. The detectable sample is introduced to and retained in the lumen by capillary forces. In a method of incubating the sample, a second liquid is introduced into the capillary containing the sample. A detection and recovery system includes an optical system for detecting the sample, and a recovery mechanism adapted to contact a capillary containing the sample. The recovery mechanism is further adapted to recover the sample from the capillary, and expel the recovered sample for analysis.

    High throughput screening for sequences of interest
    2.
    发明申请
    High throughput screening for sequences of interest 审中-公开

    公开(公告)号：US20030077677A1

    公开(公告)日：2003-04-24

    申请号：US10145281

    申请日：2002-05-13

    申请人： Diversa Corporation, a Delaware corporation

    发明人： Jay M. Short ,  Martin Keller

    IPC分类号： C12N001/20 ,  G01N033/554 ,  G01N033/569

    CPC分类号： C12N15/1034 ,  C12N1/20 ,  C12N11/04 ,  C12N15/1086 ,  C12Q1/04 ,  C40B40/08 ,  C40B50/06 ,  G01N33/56911

    摘要： Provided is a method of screening or enriching a sample containing polynucleotides from a mixed population of organisms. The method includes creating a DNA library from a plurality of nucleic acid sequences of a mixed population of organisms and separating clones containing a polynucleotide sequence of interest on a fluorescence analyzer based upon hybridization of a fluorescence labeled oligonucleotide probe to a target sequence in a clone. The separated or enrich library can then be further process by activity based screening or sequence based screening. In addition, the enriched sequence can be compared to a database and to identify sequences in the database which have homology to a clone in the library thereby obtaining a nucleic acid profile of the mixed population of organisms.

    High throughput screening for sequences of interest
    4.
    发明申请
    High throughput screening for sequences of interest 审中-公开
    感兴趣的序列的高通量筛选

    公开(公告)号：US20030077620A1

    公开(公告)日：2003-04-24

    申请号：US10145280

    申请日：2002-05-13

    申请人： Diversa Corporation, a Delaware Corporation

    发明人： Jay M. Short ,  Martin Keller

    IPC分类号： C12Q001/68

    CPC分类号： C12N15/1034 ,  C12N1/20 ,  C12N11/04 ,  C12N15/1086 ,  C12Q1/04 ,  C40B40/08 ,  C40B50/06 ,  G01N33/56911

    摘要： Provided is a method of screening or enriching a sample containing polynucleotides from a mixed population of organisms. The method includes creating a DNA library from a plurality of nucleic acid sequences of a mixed population of organisms and separating clones containing a polynucleotide sequence of interest on a fluorescence analyzer based upon hybridization of a fluorescence labeled oligonucleotide probe to a target sequence in a clone. The separated or enrich library can then be further process by activity based screening or sequence based screening. In addition, the enriched sequence can be compared to a database and to identify sequences in the database which have homology to a clone in the library thereby obtaining a nucleic acid profile of the mixed population of organisms.

    摘要翻译： 提供了筛选或富集来自生物体的混合群体的含有多核苷酸的样品的方法。 该方法包括基于荧光标记的寡核苷酸探针与克隆中的靶序列的杂交，在荧光分析仪上从多个生物体的多个核酸序列产生DNA文库和分离含有多核苷酸序列的克隆。 然后可以通过基于活性的筛选或基于序列的筛选进一步处理分离或富集的文库。 此外，可将富集的序列与数据库进行比较，并鉴定数据库中与文库中的克隆具有同源性的序列，从而获得混合生物群体的核酸谱。

    High throughput screening for novel enzymes
    5.
    发明申请
    High throughput screening for novel enzymes 有权

    公开(公告)号：US20020001809A1

    公开(公告)日：2002-01-03

    申请号：US09848095

    申请日：2001-05-03

    申请人： Diversa Corporation, a Delaware Corporation

    发明人： Jay M. Short ,  Martin Keller

    IPC分类号： C12Q001/68 ,  G01N033/53 ,  G01N033/537 ,  G01N033/543

    CPC分类号： C40B40/02 ,  C12N15/1037 ,  C12N15/1055 ,  C12Q1/6811

    摘要： Disclosed is a process for identifying clones having a specified activity of interest, which process comprises (i) generating one or more expression libraries derived from nuclei acid directly isolated from the environment; and (ii) screening said libraries utilizing a fluorescence activated cell sorter to identify said clones. More particularly, this is a process for identifying clones having a specified activity of interest by (i) generating one or more expression libraries derived from nucleic acid directly or indirectly isolated from the environment; (ii) exposing said libraries to a particular substrate or substrates of interest; and (iii) screening said exposed libraries utilizing a fluorescence activated cell sorter to identify clones which react with the substrate or substrates. Also provided is a process for identifying clones having a specified activity of interest by (i) generating one or more expression libraries derived from nucleic acid directly or indirectly isolated from the environment; and (ii) screening said exposed libraries utilizing an assay requiring co-encapsulation, a binding event or the covalent modification of a target, and a fluorescence activated cell sorter to identify positive clones.

    High throughput screening for sequences of interest
    6.
    发明申请
    High throughput screening for sequences of interest 审中-公开

    公开(公告)号：US20030092022A1

    公开(公告)日：2003-05-15

    申请号：US10145314

    申请日：2002-05-13

    申请人： Diversa Corporation, a Delaware corporation

    发明人： Jay M. Short ,  Martin Keller

    IPC分类号： C12Q001/68

    CPC分类号： C12N15/1034 ,  C12N1/20 ,  C12N11/04 ,  C12N15/1086 ,  C12Q1/04 ,  C40B40/08 ,  C40B50/06 ,  G01N33/56911

    摘要： Provided is a method of screening or enriching a sample containing polynucleotides from a mixed population of organisms. The method includes creating a DNA library from a plurality of nucleic acid sequences of a mixed population of organisms and separating clones containing a polynucleotide sequence of interest on a fluorescence analyzer based upon hybridization of a fluorescence labeled oligonucleotide probe to a target sequence in a clone. The separated or enrich library can then be further process by activity based screening or sequence based screening. In addition, the enriched sequence can be compared to a database and to identify sequences in the database which have homology to a clone in the library thereby obtaining a nucleic acid profile of the mixed population of organisms.