公开(公告)号：US10194825B2

公开(公告)日：2019-02-05

申请号：US14973542

申请日：2015-12-17

申请人： Edward R Flynn

发明人： Edward R Flynn

IPC分类号： A61B5/05 ,  C07K16/40 ,  C07K16/28 ,  C07K16/30 ,  C07K16/18 ,  A61K41/00 ,  A61B5/04 ,  A61B5/06 ,  A61B5/00 ,  A61K49/18 ,  A61B90/00

摘要： Embodiments of the present invention provide methods of detecting disease, methods of treating disease using targeted hyperthermia, methods of treating disease using targeted chemical agents, methods of treating disease comprising accurate measurements of the efficacy of treatments. The effect of nanoparticles on magnetic fields can be used to determine the location of a disease, and a measure of the number of cells characteristic of the disease. This location and measure can be used to guide therapy, and provide information regarding the most effective therapy to be applied. The same nanoparticles can be used to facilitate hyperthermia treatments, and to allow targeted application of chemical therapeutic agents.

公开(公告)号：US09074976B2

公开(公告)日：2015-07-07

申请号：US13385671

申请日：2012-02-29

申请人： Natalie L. Adolphi ,  Edward R. Flynn ,  Howard Bryant ,  Kimberly Butler

发明人： Natalie L. Adolphi ,  Edward R. Flynn ,  Howard Bryant ,  Kimberly Butler

IPC分类号： G01N11/12 ,  B82Y15/00 ,  G01N11/00

CPC分类号： G01N11/12 ,  B82Y15/00 ,  G01N2011/0086

摘要： A method for measuring the average viscosity of a test fluid uses calibrated magnetic nanoparticles, with certain chosen hydrodynamic diameters and actual lateral dimensions (e.g. diameters), that are mixed into a small volume of the test fluid and a single magnetic relaxation curve measurement to provide data for viscosity determination. The distribution of hydrodynamic particle sizes of an ensemble of magnetic nanoparticles that are magnetically blocked at room temperature can be determined. Modifications of the method can be used to determine the distribution of viscosities in a complex fluid at the sub-microscopic level providing a novel type of viscosity measurement.

公开(公告)号：US08999650B2

公开(公告)日：2015-04-07

申请号：US13399733

申请日：2012-02-17

申请人： Edward R. Flynn ,  Richard Larson

发明人： Edward R. Flynn ,  Richard Larson

IPC分类号： G01N33/53 ,  A61B10/02 ,  A61B19/00 ,  A61B17/00

CPC分类号： A61B10/0233 ,  A61B90/39 ,  A61B2017/00876 ,  A61B2090/3908 ,  A61B2090/3954

摘要： An apparatus and method for performing biopsies in-vivo using magnetically labeled nanoparticles is disclosed. One embodiment of the apparatus is called a magnetic needle. When used in a biopsy, one embodiment of the present invention collects diseased cells in-vivo which have been tagged with magnetic nanoparticles coated with receptors for specific diseased cells.

摘要翻译： 公开了一种使用磁性标记的纳米颗粒在体内进行活组织检查的装置和方法。 该装置的一个实施例称为磁针。 当用于活检时，本发明的一个实施方案在体内收集已经用特异性病变细胞的受体包被的磁性纳米颗粒标记的患病细胞。

公开(公告)号：US20140322137A1

公开(公告)日：2014-10-30

申请号：US13870925

申请日：2013-04-25

申请人： Edward R. Flynn

发明人： Edward R. Flynn

IPC分类号： A61K49/18 ,  A61B5/05 ,  G01N33/569

CPC分类号： G01N33/587 ,  A61B5/0515

摘要： The present invention can provide a method of determining the presence, location, quantity, or a combination thereof, of a biological substance, comprising: (a) exposing a sample to a plurality of targeted nanoparticles, where each targeted nanoparticle comprises a paramagnetic nanoparticle conjugated with one or more targeting agents that preferentially bind with the biological substance, under conditions that facilitate binding of the targeting agent to at least one of the one or more biological substances; (b) subjecting the sample to a magnetic field of sufficient strength to induce magnetization of the nanoparticles; (c) measuring a magnetic field of the sample after decreasing the magnetic field applied in step b below a threshold; (d) determining the presence, location, quantity, or a combination thereof, of the one or more biologic substances from the magnetic field measured in step (c).

摘要翻译： 本发明可以提供确定生物物质的存在，位置，数量或其组合的方法，其包括：（a）将样品暴露于多个靶向纳米颗粒，其中每个靶向纳米颗粒包含共轭的顺磁性纳米颗粒 与一种或多种优先与生物物质结合的靶向剂，在促进靶向剂与至少一种一种或多种生物物质结合的条件下; （b）使样品经受足够强度的磁场以引起纳米颗粒的磁化; （c）在将步骤b中施加的磁场降低到阈值之前，测量样品的磁场; （d）从步骤（c）中测量的磁场确定一种或多种生物物质的存在，位置，数量或其组合。

公开(公告)号：US20130090545A1

公开(公告)日：2013-04-11

申请号：US13703051

申请日：2011-06-07

申请人： Edward R. Flynn

发明人： Edward R. Flynn

IPC分类号： A61B5/05 ,  A61N2/12 ,  A61B5/00

CPC分类号： A61B5/05 ,  A61B5/4836 ,  A61B5/4839 ,  A61B5/4848 ,  A61K41/0052 ,  A61K49/1851 ,  A61K49/1875 ,  A61N1/403 ,  A61N2/02 ,  A61N2/12

摘要： Embodiments of the present invention provide methods of detecting cancer, methods of treating cancer using targeted hyperthermia, methods of treating cancer using targeted chemical agents, methods of treating cancer comprising accurate measurements of the efficacy of treatments. The effect of nanoparticles on magnetic fields can be used to determine the location of a tumor, and a measure of the number of cells in the tumor. This location and measure can be used to guide therapy, and provide information regarding the most effective therapy to be applied. The same nanoparticles can be used to facilitate hyperthermia treatments, and to allow targeted application of chemical therapeutic agents.

摘要翻译： 本发明的实施方案提供了检测癌症的方法，使用靶向高热治疗癌症的方法，使用靶向化学药剂治疗癌症的方法，治疗癌症的方法，包括治疗效果的精确测量。 纳米颗粒对磁场的影响可用于确定肿瘤的位置以及肿瘤中细胞数量的测量。 该位置和措施可用于指导治疗，并提供有关最有效治疗的信息。 可以使用相同的纳米颗粒来促进热疗治疗，并允许靶向施用化学治疗剂。

公开(公告)号：US20150250403A1

公开(公告)日：2015-09-10

申请号：US14702977

申请日：2015-05-04

申请人： Edward R. Flynn

发明人： Edward R. Flynn

IPC分类号： A61B5/05

CPC分类号： A61B5/0515 ,  A61B5/04005 ,  A61B5/06 ,  A61B5/415 ,  A61B5/416 ,  A61B5/418 ,  A61B2090/3954 ,  A61B2562/0285 ,  G01R33/26

摘要： The present invention provides methods and apparatuses for detecting, measuring, or locating cells or substances present in even very low concentrations in vivo in subjects, using targeted magnetic nanoparticles and special magnetic systems. The magnetic systems can comprise magnetizing subsystems and sensors subsystems, including as examples SQUID sensors and atomic magnetometers. The magnetic systems can detect, measure, or location particles bound by antibodies to cells or substances of predetermined types. Example magnetic systems are capable of detecting sub-nanogram amounts of these nanoparticles.

摘要翻译： 本发明提供了用于使用靶磁性纳米颗粒和特殊磁系统来检测，测量或定位体内甚至非常低的体内浓度的细胞或物质的方法和装置。 磁性系统可以包括磁化子系统和传感器子系统，包括例如SQUID传感器和原子磁强计。 磁性系统可以检测，测量或定位由细胞或预定类型的物质的抗体结合的颗粒。 示例磁系统能够检测亚纳米量的这些纳米颗粒。

公开(公告)号：US09095270B2

公开(公告)日：2015-08-04

申请号：US13503674

申请日：2010-11-05

申请人： Edward R. Flynn

发明人： Edward R. Flynn

IPC分类号： A61B5/05 ,  A61B5/04 ,  A61B5/06 ,  A61B5/00 ,  G01R33/26 ,  A61B19/00

CPC分类号： A61B5/0515 ,  A61B5/04005 ,  A61B5/06 ,  A61B5/415 ,  A61B5/416 ,  A61B5/418 ,  A61B2090/3954 ,  A61B2562/0285 ,  G01R33/26

摘要： The present invention provides methods and apparatuses for detecting, measuring, or locating cells or substances present in even very low concentrations in vivo in subjects, using targeted magnetic nanoparticles and special magnetic systems. The magnetic systems can comprise magnetizing subsystems and sensors subsystems, including as examples SQUID sensors and atomic magnetometers. The magnetic systems can detect, measure, or location particles bound by antibodies to cells or substances of predetermined types. Example magnetic systems are capable of detecting sub-nanogram amounts of these nanoparticles.

摘要翻译： 本发明提供了用于使用靶磁性纳米颗粒和特殊磁系统来检测，测量或定位体内甚至非常低的体内浓度的细胞或物质的方法和装置。 磁性系统可以包括磁化子系统和传感器子系统，包括例如SQUID传感器和原子磁强计。 磁性系统可以检测，测量或定位由细胞或预定类型的物质的抗体结合的颗粒。 示例磁系统能够检测亚纳米量的这些纳米颗粒。

公开(公告)号：US20130289383A1

公开(公告)日：2013-10-31

申请号：US13871683

申请日：2013-04-26

申请人： Edward R. Flynn ,  Victor Gerald Grafe

发明人： Edward R. Flynn ,  Victor Gerald Grafe

IPC分类号： A61B5/05

CPC分类号： A61B5/05 ,  A61B5/0515

摘要： The present invention can provide a method of determining the communication of substances between a first region and a second region of a patient's body. An example method according to the present invention can comprise: (a) introducing into the first region a plurality of superparamagnetic nanoparticles, having properties such that they undergo Brownian motion that randomizes the orientation of the nanoparticles according to a predetermined characteristic time; (b) after a time sufficient to allow transport of nanoparticles from the first region to the second region, subjecting the second region to an applied magnetic field of sufficient strength to induce magnetization of individual nanoparticles, and having a substantially uniform direction throughout the second region; (c) measuring the magnetic field of the second region at a plurality of times after ceasing application of the magnetic field; (d) analyzing the measured magnetic field to detect signals that correspond to decay of the magnetic field due to randomization of the nanoparticles' orientation by Brownian motion; (e) determining the presence of nanoparticles in the second region from the signals detected in step (d).

摘要翻译： 本发明可以提供一种确定患者身体的第一区域和第二区域之间的物质的通信的方法。 根据本发明的示例性方法可以包括：（a）将多个超顺磁性纳米颗粒引入第一区域，其具有使得它们经历布朗运动的性质，使得根据预定特征时间随机化纳米颗粒的取向; （b）在足以允许纳米颗粒从第一区域运输到第二区域的时间之后，使第二区域经受足够强度的施加磁场，以引起单个纳米颗粒的磁化，并且在整个第二区域具有基本均匀的方向 ; （c）在停止施加磁场之后多次测量第二区域的磁场; （d）分析测量的磁场以检测由于通过布朗运动随机化纳米颗粒取向而对应于磁场衰减的信号; （e）从步骤（d）中检测到的信号确定第二区域中纳米颗粒的存在。

公开(公告)号：US20120149029A1

公开(公告)日：2012-06-14

申请号：US13399733

申请日：2012-02-17

申请人： Edward R. Flynn ,  Richard Larson

发明人： Edward R. Flynn ,  Richard Larson

IPC分类号： G01N33/53 ,  A61B10/02

CPC分类号： A61B10/0233 ,  A61B90/39 ,  A61B2017/00876 ,  A61B2090/3908 ,  A61B2090/3954

摘要： An apparatus and method for performing biopsies in-vivo using magnetically labeled nanoparticles is disclosed. One embodiment of the apparatus is called a magnetic needle. When used in a biopsy, one embodiment of the present invention collects diseased cells in-vivo which have been tagged with magnetic nanoparticles coated with receptors for specific diseased cells.

摘要翻译： 公开了一种使用磁性标记的纳米颗粒在体内进行活组织检查的装置和方法。 该装置的一个实施例称为磁针。 当用于活检时，本发明的一个实施方案在体内收集已经用特异性病变细胞的受体包被的磁性纳米颗粒标记的患病细胞。

公开(公告)号：US11136543B1

公开(公告)日：2021-10-05

申请号：US17023084

申请日：2020-09-16

申请人： Edward R. Flynn

发明人： Edward R. Flynn

IPC分类号： C12M1/34 ,  G01N35/00 ,  A61B5/0515 ,  G01N27/72

摘要： A compact device for measuring incubation of living cells with molecules attached to superparamagnetic nanoparticles consisting of iron cores by magnetizing the nanoparticles with an external pulsed field, measuring the time dependence of decaying magnetic fields of the nanoparticles as they attach to the cells by a magnetic sensor, extracting number of attached nanoparticles per cell and rate of incubation by mathematical analysis of the magnetic field emitted by the incubating cells versus time.