公开(公告)号：US08980568B2

公开(公告)日：2015-03-17

申请号：US11264413

申请日：2005-10-31

Applicant: Ping Lin ,  Douglas T. Yamanishi ,  Paul G. Hujsak ,  Sara F. Snyder ,  George Walker ,  Junquan Xu ,  Mingxian Huang ,  Guoliang Tao ,  Lei Wu ,  Xiaobo Wang ,  Joe Ouyang ,  Charina Schmitigal ,  Jing Cheng ,  Elizabeth Kwok ,  Jia Xu

Inventor： Ping Lin ,  Douglas T. Yamanishi ,  Paul G. Hujsak ,  Sara F. Snyder ,  George Walker ,  Junquan Xu ,  Mingxian Huang ,  Guoliang Tao ,  Lei Wu ,  Xiaobo Wang ,  Joe Ouyang ,  Charina Schmitigal ,  Jing Cheng ,  Elizabeth Kwok ,  Jia Xu

IPC: C12Q1/24 ,  G01N33/574 ,  B01D71/04 ,  B01D71/02 ,  G01N1/40 ,  G01N15/05 ,  B01D67/00 ,  B01D57/02 ,  A61M1/36 ,  G01N33/569 ,  G01N15/02 ,  G01N1/34 ,  B01L3/00 ,  C12Q1/68 ,  B01D61/18 ,  G01N15/00 ,  G01N15/06

CPC classification number: G01N1/4077 ,  A61B5/150755 ,  A61M1/36 ,  A61M1/3618 ,  A61M1/362 ,  A61M1/3679 ,  B01D57/02 ,  B01D61/18 ,  B01D67/0034 ,  B01D67/0062 ,  B01D71/02 ,  B01D71/027 ,  B01D71/04 ,  B01D2325/021 ,  B01L3/502 ,  B01L3/5027 ,  B01L3/502753 ,  B01L3/502761 ,  B01L2200/10 ,  B01L2300/0627 ,  B01L2300/0681 ,  B01L2300/0816 ,  B01L2300/0861 ,  B01L2400/0409 ,  B01L2400/0415 ,  B01L2400/0457 ,  B01L2400/0478 ,  B01L2400/0644 ,  B01L2400/065 ,  C12Q1/6841 ,  C12Q1/6881 ,  G01N1/34 ,  G01N1/40 ,  G01N15/0272 ,  G01N15/05 ,  G01N33/56966 ,  G01N33/57407 ,  G01N35/026 ,  G01N35/04 ,  G01N35/1002 ,  G01N2001/4016 ,  G01N2001/4083 ,  G01N2001/4088 ,  G01N2015/0073 ,  G01N2015/0288 ,  G01N2015/0294 ,  G01N2015/0681 ,  G01N2035/0406 ,  G01N2333/4724 ,  Y10T436/25375

Abstract: The present invention recognizes that diagnosis and prognosis of many conditions can depend on the enrichment of rare cells, especially tumor cells, from a complex fluid sample such as a blood sample. In particular, the present invention is directed to methods and compositions for detecting a non-hematopoietic cell, e.g., a non-hematopoietic tumor cell, in a blood sample via, inter alia, removing red blood cells (RBCs) from a blood sample using a non-centrifugation procedure, removing white blood cells (WBCs) from said blood sample to enrich a non-hematopoietic cell, if any, from said blood sample; and assessing the presence, absence and/or amount of said enriched non-hematopoietic cell.