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公开(公告)号：US08980568B2
公开(公告)日：2015-03-17
申请号：US11264413
申请日：2005-10-31
申请人： Ping Lin , Douglas T. Yamanishi , Paul G. Hujsak , Sara F. Snyder , George Walker , Junquan Xu , Mingxian Huang , Guoliang Tao , Lei Wu , Xiaobo Wang , Joe Ouyang , Charina Schmitigal , Jing Cheng , Elizabeth Kwok , Jia Xu
发明人： Ping Lin , Douglas T. Yamanishi , Paul G. Hujsak , Sara F. Snyder , George Walker , Junquan Xu , Mingxian Huang , Guoliang Tao , Lei Wu , Xiaobo Wang , Joe Ouyang , Charina Schmitigal , Jing Cheng , Elizabeth Kwok , Jia Xu
IPC分类号： C12Q1/24 , G01N33/574 , B01D71/04 , B01D71/02 , G01N1/40 , G01N15/05 , B01D67/00 , B01D57/02 , A61M1/36 , G01N33/569 , G01N15/02 , G01N1/34 , B01L3/00 , C12Q1/68 , B01D61/18 , G01N15/00 , G01N15/06
CPC分类号： G01N1/4077 , A61B5/150755 , A61M1/36 , A61M1/3618 , A61M1/362 , A61M1/3679 , B01D57/02 , B01D61/18 , B01D67/0034 , B01D67/0062 , B01D71/02 , B01D71/027 , B01D71/04 , B01D2325/021 , B01L3/502 , B01L3/5027 , B01L3/502753 , B01L3/502761 , B01L2200/10 , B01L2300/0627 , B01L2300/0681 , B01L2300/0816 , B01L2300/0861 , B01L2400/0409 , B01L2400/0415 , B01L2400/0457 , B01L2400/0478 , B01L2400/0644 , B01L2400/065 , C12Q1/6841 , C12Q1/6881 , G01N1/34 , G01N1/40 , G01N15/0272 , G01N15/05 , G01N33/56966 , G01N33/57407 , G01N35/026 , G01N35/04 , G01N35/1002 , G01N2001/4016 , G01N2001/4083 , G01N2001/4088 , G01N2015/0073 , G01N2015/0288 , G01N2015/0294 , G01N2015/0681 , G01N2035/0406 , G01N2333/4724 , Y10T436/25375
摘要： The present invention recognizes that diagnosis and prognosis of many conditions can depend on the enrichment of rare cells, especially tumor cells, from a complex fluid sample such as a blood sample. In particular, the present invention is directed to methods and compositions for detecting a non-hematopoietic cell, e.g., a non-hematopoietic tumor cell, in a blood sample via, inter alia, removing red blood cells (RBCs) from a blood sample using a non-centrifugation procedure, removing white blood cells (WBCs) from said blood sample to enrich a non-hematopoietic cell, if any, from said blood sample; and assessing the presence, absence and/or amount of said enriched non-hematopoietic cell.
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2.发明授权公开(公告)号：US08986944B2
公开(公告)日：2015-03-24
申请号：US11497919
申请日：2006-08-02
申请人： Douglas T. Yamanishi , Paul G. Hujsak , Sara F. Snyder , George Walker , Junquan Xu , Mingxian Huang , Guoliang Tao , Lei Wu , Xiaobo Wang , Joe Ouyang , Charina Schmitigal , Jing Cheng , Jia Xu
发明人： Douglas T. Yamanishi , Paul G. Hujsak , Sara F. Snyder , George Walker , Junquan Xu , Mingxian Huang , Guoliang Tao , Lei Wu , Xiaobo Wang , Joe Ouyang , Charina Schmitigal , Jing Cheng , Jia Xu
IPC分类号： C12Q1/24 , C12Q1/68 , G01N1/40 , G01N33/569 , G01N33/574 , B01L3/00
CPC分类号： C12Q1/6881 , B01L3/5027 , C12N5/0602 , G01N1/40 , G01N1/4077 , G01N33/56966 , G01N33/57407 , G01N2333/4724 , Y10T436/25375
摘要： The present invention includes methods of enriching rare cells, such as cancer cells, from biological samples, such as blood samples. The methods include performing at least one debulking step on a blood sample and selectively removing at least one type undesirable component from the blood sample to obtain a blood sample that is enriched in a rare cell of interest. In some embodiments magnetic beads coupled to specific binding members are used to selectively removed components.
摘要翻译： 本发明包括从诸如血液样品的生物样品中富集稀有细胞如癌细胞的方法。 所述方法包括对血液样品进行至少一个减压步骤，并从血液样品中选择性地除去至少一种不需要的组分以获得富含稀有细胞的血液样品。 在一些实施例中，耦合到特定结合构件的磁珠用于选择性地去除组分。
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3.发明申请公开(公告)号：US20070202536A1
公开(公告)日：2007-08-30
申请号：US11497919
申请日：2006-08-02
申请人： Douglas Yamanishi , Paul Hujsak , Sara Snyder , George Walker , Junquan Xu , Mingxian Huang , Guoliang Tao , Lei Wu , Xiaobo Wang , Joe Ouyang , Charina Schmitigal , Samantha O'Neill , Jing Cheng , Jia Xu
发明人： Douglas Yamanishi , Paul Hujsak , Sara Snyder , George Walker , Junquan Xu , Mingxian Huang , Guoliang Tao , Lei Wu , Xiaobo Wang , Joe Ouyang , Charina Schmitigal , Samantha O'Neill , Jing Cheng , Jia Xu
CPC分类号： C12Q1/6881 , B01L3/5027 , C12N5/0602 , G01N1/40 , G01N1/4077 , G01N33/56966 , G01N33/57407 , G01N2333/4724 , Y10T436/25375
摘要： The present invention includes methods of enriching rare cells, such as cancer cells, from biological samples, such as blood samples. The methods include performing at least one debulking step on a blood sample and selectively removing at least one type undesirable component from the blood sample to obtain a blood sample that is enriched in a rare cell of interest. In some embodiments magnetic beads coupled to specific binding members are used to selectively removed components.
摘要翻译： 本发明包括从诸如血液样品的生物样品中富集稀有细胞如癌细胞的方法。 所述方法包括对血液样品进行至少一个减压步骤，并从血液样品中选择性地除去至少一种不需要的组分以获得富含稀有细胞的血液样品。 在一些实施例中，耦合到特定结合构件的磁珠用于选择性地去除组分。
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公开(公告)号：US20060252054A1
公开(公告)日：2006-11-09
申请号：US11264413
申请日：2005-10-31
申请人： Ping Lin , Douglas Yamanishi , Paul Hujsak , Sara Snyder , George Walker , Junguan Xu , Mingxian Huang , Guoliang Tao , Lei Wu , Xiaobo Wang , Joe Ouyang , Charina Schmitigal , Samantha O'Neill , Jing Cheng , Elizabeth Voung , Jia Xu
发明人： Ping Lin , Douglas Yamanishi , Paul Hujsak , Sara Snyder , George Walker , Junguan Xu , Mingxian Huang , Guoliang Tao , Lei Wu , Xiaobo Wang , Joe Ouyang , Charina Schmitigal , Samantha O'Neill , Jing Cheng , Elizabeth Voung , Jia Xu
IPC分类号： C12Q1/68 , G01N33/574
CPC分类号： G01N1/4077 , A61B5/150755 , A61M1/36 , A61M1/3618 , A61M1/362 , A61M1/3679 , B01D57/02 , B01D61/18 , B01D67/0034 , B01D67/0062 , B01D71/02 , B01D71/027 , B01D71/04 , B01D2325/021 , B01L3/502 , B01L3/5027 , B01L3/502753 , B01L3/502761 , B01L2200/10 , B01L2300/0627 , B01L2300/0681 , B01L2300/0816 , B01L2300/0861 , B01L2400/0409 , B01L2400/0415 , B01L2400/0457 , B01L2400/0478 , B01L2400/0644 , B01L2400/065 , C12Q1/6841 , C12Q1/6881 , G01N1/34 , G01N1/40 , G01N15/0272 , G01N15/05 , G01N33/56966 , G01N33/57407 , G01N35/026 , G01N35/04 , G01N35/1002 , G01N2001/4016 , G01N2001/4083 , G01N2001/4088 , G01N2015/0073 , G01N2015/0288 , G01N2015/0294 , G01N2015/0681 , G01N2035/0406 , G01N2333/4724 , Y10T436/25375
摘要： The present invention recognizes that diagnosis and prognosis of many conditions can depend on the enrichment of rare cells, especially tumor cells, from a complex fluid sample such as a blood sample. In particular, the present invention is directed to methods and compositions for detecting a non-hematopoietic cell, e.g., a non-hematopoietic tumor cell, in a blood sample via, inter alia, removing red blood cells (RBCs) from a blood sample using a non-centrifugation procedure, removing white blood cells (WBCs) from said blood sample to enrich a non-hematopoietic cell, if any, from said blood sample; and assessing the presence, absence and/or amount of said enriched non-hematopoietic cell.
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5.发明授权公开(公告)号：US07718419B2
公开(公告)日：2010-05-18
申请号：US11841935
申请日：2007-08-20
申请人： Lei Wu , Xiaobo Wang , Guoliang Tao , Junquan Xu , Jing Cheng , Mingxian Huang , Baoquan Sun , Wei Shao , Litian Liu , Depu Chen , David M. Rothwarf , Weiping Yang
发明人： Lei Wu , Xiaobo Wang , Guoliang Tao , Junquan Xu , Jing Cheng , Mingxian Huang , Baoquan Sun , Wei Shao , Litian Liu , Depu Chen , David M. Rothwarf , Weiping Yang
IPC分类号： C12M1/34 , G01N33/553
CPC分类号： C40B40/12 , B01J19/0046 , B01J2219/00457 , B01J2219/00468 , B01J2219/00497 , B01J2219/005 , B01J2219/00502 , B01J2219/0054 , B01J2219/00542 , B01J2219/00547 , B01J2219/00549 , B01J2219/00556 , B01J2219/00576 , B01J2219/00585 , B01J2219/0059 , B01J2219/00592 , B01J2219/00596 , B01J2219/00655 , B01J2219/00659 , B01J2219/00702 , B01J2219/00707 , B01J2219/0072 , B01J2219/00722 , B01J2219/00725 , B01J2219/00729 , B01J2219/00731 , B01J2219/00734 , B01J2219/0074 , B01J2219/00743 , B01L3/5027 , B01L3/50273 , B01L3/502761 , B01L2200/0647 , B01L2300/0819 , B01L2400/0415 , B01L2400/043 , B03C5/028 , B82Y5/00 , B82Y10/00 , B82Y30/00 , C07B2200/11 , C07K1/047 , C40B40/06 , C40B40/10 , G01N33/54366 , G01N33/54386 , G06K19/06009 , G06K19/06046 , G06K19/06196 , Y10T428/24917
摘要： This invention relates generally to the field of moiety or molecule isolation, detection and manipulation and library synthesis. In particular, the invention provides a microdevice, which microdevice comprises: a) a magnetizable substance; and b) a photorecognizable coding pattern, wherein said microdevice has a preferential axis of magnetization. Systems and methods for isolating, detecting and manipulating moieties and synthesizing libraries using the microdevices are also provided.
摘要翻译： 本发明一般涉及部分或分子分离，检测和操作以及文库合成领域。 特别地，本发明提供一种微型装置，该微型装置包括：a）可磁化物质; 以及b）可识别的编码图案，其中所述微器件具有优先磁化轴。 还提供了用于使用微器件分离，检测和操作部分和合成文库的系统和方法。
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6.发明申请MICRODEVICES CONTAINING PHOTORECOGNIZABLE CODING PATTERNS AND METHODS OF USING AND PRODUCING THE SAME 审中-公开包含可光化编码图案的微型装置及其使用和生产方法公开(公告)号：US20100260984A1
公开(公告)日：2010-10-14
申请号：US12760379
申请日：2010-04-14
申请人： Lei WU , Xiaobo Wang , Guoliang Tao , Junquan Xu , Jing Cheng , Mingxian Huang , Baoquan Sun , Wei Shao , Litian Liu , Depu Chen , David M. Rothwarf , Weiping Yang
发明人： Lei WU , Xiaobo Wang , Guoliang Tao , Junquan Xu , Jing Cheng , Mingxian Huang , Baoquan Sun , Wei Shao , Litian Liu , Depu Chen , David M. Rothwarf , Weiping Yang
IPC分类号： B32B3/00
CPC分类号： C40B40/12 , B01J19/0046 , B01J2219/00457 , B01J2219/00468 , B01J2219/00497 , B01J2219/005 , B01J2219/00502 , B01J2219/0054 , B01J2219/00542 , B01J2219/00547 , B01J2219/00549 , B01J2219/00556 , B01J2219/00576 , B01J2219/00585 , B01J2219/0059 , B01J2219/00592 , B01J2219/00596 , B01J2219/00655 , B01J2219/00659 , B01J2219/00702 , B01J2219/00707 , B01J2219/0072 , B01J2219/00722 , B01J2219/00725 , B01J2219/00729 , B01J2219/00731 , B01J2219/00734 , B01J2219/0074 , B01J2219/00743 , B01L3/5027 , B01L3/50273 , B01L3/502761 , B01L2200/0647 , B01L2300/0819 , B01L2400/0415 , B01L2400/043 , B03C5/028 , B82Y5/00 , B82Y10/00 , B82Y30/00 , C07B2200/11 , C07K1/047 , C40B40/06 , C40B40/10 , G01N33/54366 , G01N33/54386 , G06K19/06009 , G06K19/06046 , G06K19/06196 , Y10T428/24917
摘要： This invention relates generally to the field of moiety or molecule analysis, isolation, detection and manipulation and library synthesis. In particular, the invention provides a microdevice, which microdevice comprises: a) a substrate; and b) a photorecognizable coding pattern on said substrate. Preferably, the microdevice does not comprise an anodized metal surface layer. Methods and kits for isolating, detecting and manipulating moieties, and synthesizing libraries using the microdevices are also provided. The invention further provides two-dimensional optical encoders and uses thereof. In certain embodiments, the invention provides a microdevice, which microdevice comprises: a) a magnetizable substance; and b) a photorecognizable coding pattern, wherein said microdevice has a preferential axis of magnetization. Systems and methods for isolating, detecting and manipulating moieties and synthesizing libraries using the microdevices are also provided.
摘要翻译： 本发明一般涉及部分或分子分析，分离，检测和操作以及文库合成领域。 特别地，本发明提供了一种微型器件，该微型器件包括：a）衬底; 和b）在所述衬底上的可识别的编码图案。 优选地，微器件不包括阳极化金属表面层。 还提供了用于分离，检测和操作部分以及使用微器件合成文库的方法和试剂盒。 本发明还提供二维光学编码器及其用途。 在某些实施方案中，本发明提供一种微型装置，该微型装置包括：a）可磁化物质; 以及b）可识别的编码图案，其中所述微器件具有优先磁化轴。 还提供了用于使用微器件分离，检测和操作部分和合成文库的系统和方法。
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7.发明申请MICRODEVICES CONTAINING PHOTORECOGNIZABLE CODING PATTERNS AND METHODS OF USING AND PRODUCING THE SAME 审中-公开包含可光化编码图案的微型装置及其使用和生产方法公开(公告)号：US20120228386A1
公开(公告)日：2012-09-13
申请号：US13476809
申请日：2012-05-21
申请人： Lei WU , Xiaobo Wang , Guoliang Tao , Junquan Xu , Jing Cheng , Mingxian Huang , Baoquan Sun , Wei Shao , Litian Liu , Depu Chen , David M. Rothwarf , Weiping Yang
发明人： Lei WU , Xiaobo Wang , Guoliang Tao , Junquan Xu , Jing Cheng , Mingxian Huang , Baoquan Sun , Wei Shao , Litian Liu , Depu Chen , David M. Rothwarf , Weiping Yang
CPC分类号： C40B40/12 , B01J19/0046 , B01J2219/00457 , B01J2219/00468 , B01J2219/00497 , B01J2219/005 , B01J2219/00502 , B01J2219/0054 , B01J2219/00542 , B01J2219/00547 , B01J2219/00549 , B01J2219/00556 , B01J2219/00576 , B01J2219/00585 , B01J2219/0059 , B01J2219/00592 , B01J2219/00596 , B01J2219/00655 , B01J2219/00659 , B01J2219/00702 , B01J2219/00707 , B01J2219/0072 , B01J2219/00722 , B01J2219/00725 , B01J2219/00729 , B01J2219/00731 , B01J2219/00734 , B01J2219/0074 , B01J2219/00743 , B01L3/5027 , B01L3/50273 , B01L3/502761 , B01L2200/0647 , B01L2300/0819 , B01L2400/0415 , B01L2400/043 , B03C5/028 , B82Y5/00 , B82Y10/00 , B82Y30/00 , C07B2200/11 , C07K1/047 , C40B40/06 , C40B40/10 , G01N33/54366 , G01N33/54386 , G06K19/06009 , G06K19/06046 , G06K19/06196 , Y10T428/24917
摘要： This invention relates generally to the field of moiety or molecule analysis, isolation, detection and manipulation and library synthesis. In particular, the invention provides a microdevice, which microdevice comprises: a) a substrate; and b) a photorecognizable coding pattern on said substrate. Preferably, the microdevice does not comprise an anodized metal surface layer. Methods and kits for isolating, detecting and manipulating moieties, and synthesizing libraries using the microdevices are also provided. The invention further provides two-dimensional optical encoders and uses thereof. In certain embodiments, the invention provides a microdevice, which microdevice comprises: a) a magnetizable substance; and b) a photorecognizable coding pattern, wherein said microdevice has a preferential axis of magnetization. Systems and methods for isolating, detecting and manipulating moieties and synthesizing libraries using the microdevices are also provided.
摘要翻译： 本发明一般涉及部分或分子分析，分离，检测和操作以及文库合成领域。 特别地，本发明提供了一种微型器件，该微型器件包括：a）衬底; 和b）在所述衬底上的可识别的编码图案。 优选地，微器件不包括阳极化金属表面层。 还提供了用于分离，检测和操作部分以及使用微器件合成文库的方法和试剂盒。 本发明还提供二维光学编码器及其用途。 在某些实施方案中，本发明提供一种微型装置，该微型装置包括：a）可磁化物质; 以及b）可识别的编码图案，其中所述微器件具有优先磁化轴。 还提供了用于使用微器件分离，检测和操作部分和合成文库的系统和方法。
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8.发明申请公开(公告)号：US20080286750A1
公开(公告)日：2008-11-20
申请号：US11841985
申请日：2007-08-20
申请人： Jia XU , Antonio Guia , Xiaobo Wang , Lei Wu , Junquan Xu , Mingxian Huang , David Rothwarf
发明人： Jia XU , Antonio Guia , Xiaobo Wang , Lei Wu , Junquan Xu , Mingxian Huang , David Rothwarf
IPC分类号： C12Q1/00
CPC分类号： G01N33/48728
摘要： The present invention recognizes that the determination of ion transport function or properties using direct detection methods, such as whole cell recording or single channel recording, are preferable to methods that utilize indirect detection methods, such as FRET based detection system. The present invention provides biochips and other fluidic components and methods of use that allow for the direct analysis of ion transport function or properties using microfabricated structures that can allow for automated detection of ion transport function or properties. These biochips and fluidic components and methods of use thereof are particularly appropriate for automating the detection of ion transport function or properties, particularly for screening purposes.
摘要翻译： 本发明认识到，使用诸如全细胞记录或单通道记录之类的直接检测方法的离子转运功能或性质的确定优于使用间接检测方法的方法，例如基于FRET的检测系统。 本发明提供生物芯片和其他流体组分和使用方法，其允许使用可允许自动检测离子迁移功能或性质的微结构的直接分析离子转运功能或性质。 这些生物芯片和流体组分及其使用方法特别适用于自动化离子迁移功能或性质的检测，特别是用于筛选目的。
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9.发明申请公开(公告)号：US20050009004A1
公开(公告)日：2005-01-13
申请号：US10760886
申请日：2004-01-20
申请人： Jia Xu , Antonio Guia , Xiaobo Wang , Lei Wu , Junquan Xu , Mingxian Huang , David Rothwarf
发明人： Jia Xu , Antonio Guia , Xiaobo Wang , Lei Wu , Junquan Xu , Mingxian Huang , David Rothwarf
IPC分类号： C12M1/34 , C12Q1/00 , G01N33/487
CPC分类号： G01N33/48728
摘要： The present invention recognizes that the determination of ion transport function or properties using direct detection methods, such as whole cell recording or single channel recording, are preferable to methods that utilize indirect detection methods, such as FRET based detection system. The present invention provides biochips and other fluidic components and methods of use that allow for the direct analysis of ion transport function or properties using microfabricated structures that can allow for automated detection of ion transport function or properties. These biochips and fluidic components and methods of use thereof are particularly appropriate for automating the detection of ion transport function or properties, particularly for screening purposes.
摘要翻译： 本发明认识到，使用诸如全细胞记录或单通道记录之类的直接检测方法的离子转运功能或性质的确定优于使用间接检测方法的方法，例如基于FRET的检测系统。 本发明提供生物芯片和其他流体组分和使用方法，其允许使用可允许自动检测离子迁移功能或性质的微结构的直接分析离子转运功能或性质。 这些生物芯片和流体组分及其使用方法特别适用于自动化离子迁移功能或性质的检测，特别是用于筛选目的。
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公开(公告)号：US20070160984A1
公开(公告)日：2007-07-12
申请号：US11714954
申请日：2007-03-06
申请人： Mingxian Huang , Lei Wu , Xiaobo Wang , Junquan Xu , Guo Tao , Jing Cheng
发明人： Mingxian Huang , Lei Wu , Xiaobo Wang , Junquan Xu , Guo Tao , Jing Cheng
IPC分类号： C12Q1/70 , G01N33/567 , B32B5/16 , G01N33/551
CPC分类号： C40B70/00 , B01J19/0046 , B01J2219/00459 , B01J2219/005 , B01J2219/00545 , B01J2219/00551 , B01J2219/00585 , B01J2219/00596 , B01J2219/00655 , B01J2219/0072 , B01J2219/0074 , B03C1/02 , B03C5/026 , B03C5/028 , B82Y15/00 , C40B40/06 , C40B50/16 , G01N33/54326 , G01N33/588 , Y10S428/90 , Y10T428/2982 , Y10T428/2991 , Y10T428/32
摘要： This invention relates generally to the field of moiety or molecule isolation, detection and manipulation and library synthesis. In particular, the invention provides a bead, which bead comprises: a) a magnetizable substance; and b) an electrically conductive substance or an optical labeling substance. Methods and kits for isolating, detecting and manipulating moieties and synthesizing libraries using the beads are also provided.
摘要翻译： 本发明一般涉及部分或分子分离，检测和操作以及文库合成领域。 特别地，本发明提供了一种珠，该珠包括：a）可磁化物质; 和b）导电物质或光学标记物质。 还提供了用于分离，检测和操作部分并使用珠的合成文库的方法和试剂盒。
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