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    Methods, compositions, and automated systems for separating rare cells from fluid samples
    8.
    发明授权
    Methods, compositions, and automated systems for separating rare cells from fluid samples 有权
    用于从流体样品中分离稀有细胞的方法，组合物和自动化系统

    公开(公告)号：US07166443B2

    公开(公告)日：2007-01-23

    申请号：US10701684

    申请日：2003-11-04

    申请人： George Walker ,  Junquan Xu ,  Douglas Yamanishi ,  Paul Hujsak ,  Lei Wu ,  Mingxian Huang ,  Guoliang Tao ,  Sara Snyder ,  Charina Yap

    发明人： George Walker ,  Junquan Xu ,  Douglas Yamanishi ,  Paul Hujsak ,  Lei Wu ,  Mingxian Huang ,  Guoliang Tao ,  Sara Snyder ,  Charina Yap

    IPC分类号： C12Q1/04

    CPC分类号： B01L3/502761 ,  A61M1/36 ,  A61M1/3618 ,  A61M1/362 ,  A61M1/3679 ,  B01D57/02 ,  B01D61/18 ,  B01D67/0034 ,  B01D67/0062 ,  B01D71/02 ,  B01D71/027 ,  B01D71/04 ,  B01D2325/021 ,  B01D2325/04 ,  B01L3/502 ,  B01L3/502753 ,  B01L2300/0681 ,  B01L2300/0816 ,  B01L2300/0861 ,  B01L2400/0409 ,  B01L2400/0457 ,  B01L2400/0478 ,  B01L2400/0644 ,  B01L2400/065 ,  G01N1/40 ,  G01N15/0272 ,  G01N15/05 ,  G01N2001/4016 ,  G01N2015/0073 ,  G01N2015/0288 ,  G01N2015/0294 ,  G01N2015/0681

    摘要： The present invention recognizes that diagnosis and prognosis of many conditions can depend on the enrichment of rare cells from a complex fluid sample. In particular, the enrichment of fetal cells from maternal samples, such as maternal blood samples, can greatly aid in the detection of fetal abnormalities or a variety of genetic conditions. In addition, the present invention recognizes that the enrichment of rare malignant cells from patient samples, can aid in diagnosis, prognosis, and development of therapeutic modalities for patients. The invention includes microfabricated filters for filtering fluid samples and methods of enriching rare cells of fluid samples using microfabricated filters of the present invention. The invention also includes solutions for the selective sedimentation of red blood cells (RBCs) from a blood sample and methods of using selective RBC sedimentation solutions for enriching rare cells of a fluid sample. Yet another aspect of the invention is an automated system for processing a fluid sample that includes: at least one filtration chamber that includes a microfabricated filter; automated means for directing fluid flow through at least one filtration chamber of the automated system, and means for collecting enriched rare cells. The present invention also includes methods of using automated systems for separating rare cells from fluid samples. Preferred fluid samples are blood, effusion, or urine samples, and rare cells that can be enriched from such sample include nucleated red blood cells and cancer cells.

    摘要翻译： 本发明认识到许多病症的诊断和预后可以取决于来自复杂流体样品的稀有细胞的富集。 特别地，母体样品（例如母体血液样品）富集胎儿细胞可以大大有助于检测胎儿异常或各种遗传病症。 此外，本发明认识到，来自患者样品的稀有恶性细胞的富集可以帮助患者的治疗方式的诊断，预后和发展。 本发明包括用于过滤液体样品的微制造过滤器和使用本发明的微加工过滤器来富集稀有细胞液体样品的方法。 本发明还包括从血液样品中选择性沉降红细胞（RBC）的方案和使用选择性RBC沉降溶液富集流体样品稀有细胞的方法。 本发明的另一方面是一种用于处理流体样品的自动化系统，该系统包括：至少一个包括微加工过滤器的过滤室; 用于引导流体流过自动化系统的至少一个过滤室的自动装置，以及用于收集富集的稀有细胞的装置。 本发明还包括使用自动化系统从流体样品中分离稀有细胞的方法。 优选的流体样品是血液，渗出物或尿液样品，并且可以从这种样品中富集的稀有细胞包括成核红细胞和癌细胞。

    APPARATUS INCLUDING ION TRANSPORT DETECTING STRUCTURES AND METHODS OF USE
    9.
    发明申请
    APPARATUS INCLUDING ION TRANSPORT DETECTING STRUCTURES AND METHODS OF USE 审中-公开
    装置包括离子运输检测结构和使用方法

    公开(公告)号：US20080286750A1

    公开(公告)日：2008-11-20

    申请号：US11841985

    申请日：2007-08-20

    申请人： Jia XU ,  Antonio Guia ,  Xiaobo Wang ,  Lei Wu ,  Junquan Xu ,  Mingxian Huang ,  David Rothwarf

    发明人： Jia XU ,  Antonio Guia ,  Xiaobo Wang ,  Lei Wu ,  Junquan Xu ,  Mingxian Huang ,  David Rothwarf

    IPC分类号： C12Q1/00

    CPC分类号： G01N33/48728

    摘要： The present invention recognizes that the determination of ion transport function or properties using direct detection methods, such as whole cell recording or single channel recording, are preferable to methods that utilize indirect detection methods, such as FRET based detection system. The present invention provides biochips and other fluidic components and methods of use that allow for the direct analysis of ion transport function or properties using microfabricated structures that can allow for automated detection of ion transport function or properties. These biochips and fluidic components and methods of use thereof are particularly appropriate for automating the detection of ion transport function or properties, particularly for screening purposes.

    摘要翻译： 本发明认识到，使用诸如全细胞记录或单通道记录之类的直接检测方法的离子转运功能或性质的确定优于使用间接检测方法的方法，例如基于FRET的检测系统。 本发明提供生物芯片和其他流体组分和使用方法，其允许使用可允许自动检测离子迁移功能或性质的微结构的直接分析离子转运功能或性质。 这些生物芯片和流体组分及其使用方法特别适用于自动化离子迁移功能或性质的检测，特别是用于筛选目的。

    Apparatus including ion transport detecting structures and methods of use
    10.
    发明申请
    Apparatus including ion transport detecting structures and methods of use 审中-公开
    包括离子传输检测结构和使用方法的装置

    公开(公告)号：US20050009004A1

    公开(公告)日：2005-01-13

    申请号：US10760886

    申请日：2004-01-20

    申请人： Jia Xu ,  Antonio Guia ,  Xiaobo Wang ,  Lei Wu ,  Junquan Xu ,  Mingxian Huang ,  David Rothwarf

    发明人： Jia Xu ,  Antonio Guia ,  Xiaobo Wang ,  Lei Wu ,  Junquan Xu ,  Mingxian Huang ,  David Rothwarf

    IPC分类号： C12M1/34 ,  C12Q1/00 ,  G01N33/487

    CPC分类号： G01N33/48728

    摘要： The present invention recognizes that the determination of ion transport function or properties using direct detection methods, such as whole cell recording or single channel recording, are preferable to methods that utilize indirect detection methods, such as FRET based detection system. The present invention provides biochips and other fluidic components and methods of use that allow for the direct analysis of ion transport function or properties using microfabricated structures that can allow for automated detection of ion transport function or properties. These biochips and fluidic components and methods of use thereof are particularly appropriate for automating the detection of ion transport function or properties, particularly for screening purposes.

    摘要翻译： 本发明认识到，使用诸如全细胞记录或单通道记录之类的直接检测方法的离子转运功能或性质的确定优于使用间接检测方法的方法，例如基于FRET的检测系统。 本发明提供生物芯片和其他流体组分和使用方法，其允许使用可允许自动检测离子迁移功能或性质的微结构的直接分析离子转运功能或性质。 这些生物芯片和流体组分及其使用方法特别适用于自动化离子迁移功能或性质的检测，特别是用于筛选目的。