公开(公告)号：US5432079A

公开(公告)日：1995-07-11

申请号：US166681

申请日：1988-03-11

Applicant: Eric Johansen ,  Edward R. Appelbaum

Inventor： Eric Johansen ,  Edward R. Appelbaum

IPC: C12N15/74 ,  C12Q1/04 ,  C12Q1/68 ,  C12N1/20 ,  C12N1/21 ,  C12N15/01

CPC classification number: C12Q1/6897 ,  C12N15/743 ,  C12Q1/04 ,  Y10S435/878

Abstract: Methods for the isolation of mutant rhizobial strains with improved competition of nodulation (Comp.sup.+) is presented. Mutants are selected for constitutive expression of inducible nod genes (in the absence of inducer) and screened for hyperinduction of nod genes in the presence of inducer. Mutants which are defective in nodulation or in symbiotic nitrogen fixation are eliminated from further testing. Selections for constitutive nod expression and for hyperinduction of nod genes are facilitated by the use of plasmid carrying a selectable marker fused to an inducible nod gene and a plasmid carrying a reporter gene fused downstream of an inducible nod gene. The methods exemplified are particularly useful for the isolation and identification of Comp.sup.+ mutants of Bradyrhizobium japonicum.

Abstract translation: 提出了具有改善的结瘤竞争（Comp +）的突变根瘤菌菌株分离方法。 选择突变体用于诱导型节点基因的组成型表达（在不存在诱导物的情况下），并筛选出在诱导物存在下点头基因的高度诱导。 在结瘤或共生固氮中有缺陷的突变体从进一步测试中消除。 通过使用携带可诱导的点头基因融合的选择标记的质粒和携带可诱导的点头基因下游融合的报道基因的质粒，促进了组成型nod表达和nod基因超调诱导的选择。 所列举的方法对于分离和鉴定日本Bradyrhizobium日本的Comp +突变体是特别有用的。

公开(公告)号：US06936688B2

公开(公告)日：2005-08-30

申请号：US10125451

申请日：2002-04-19

Applicant: Henrik S. Olsen ,  Haodong Li ,  Mark D. Adams ,  Solange H. L. Gentz ,  Ralph Alderson ,  Yuling Li ,  David Parmelee ,  John R. White ,  Edward R. Appelbaum

Inventor： Henrik S. Olsen ,  Haodong Li ,  Mark D. Adams ,  Solange H. L. Gentz ,  Ralph Alderson ,  Yuling Li ,  David Parmelee ,  John R. White ,  Edward R. Appelbaum

IPC: A61K38/19 ,  C07H21/04 ,  C07K14/52 ,  C12N5/06 ,  C12N5/10 ,  C12N15/19 ,  C12N15/63 ,  C12P21/02

CPC classification number: C07K14/523 ,  A61K38/00 ,  C07K2319/00

Abstract: Human chemokine Beta-10 polypeptides and DNA (RNA) encoding such chemokine polypeptides and a procedure for producing such polypeptides by recombinant techniques is disclosed. Also disclosed are methods for utilizing such chemokine polypeptides for the treatment of leukemia, tumors, chronic infections, autoimmune disease, fibrotic disorders, wound healing and psoriasis. Antagonists against such chemokine polypeptides and their use as a therapeutic to treat rheumatoid arthritis, autoimmune and chronic inflammatory and infective diseases, allergic reactions, prostaglandin-independent fever and bone marrow failure are also disclosed.

Abstract translation: 公开了人趋化因子β-10多肽和编码这种趋化因子多肽的DNA（RNA）和通过重组技术产生这种多肽的方法。 还公开了利用这种趋化因子多肽治疗白血病，肿瘤，慢性感染，自身免疫疾病，纤维化病症，伤口愈合和牛皮癣的方法。 还公开了抗这种趋化因子多肽的拮抗剂及其作为治疗类风湿性关节炎，自身免疫性和慢性炎性和感染性疾病，过敏反应，前列腺素依赖性发烧和骨髓衰竭的治疗剂的用途。

公开(公告)号：US6153441A

公开(公告)日：2000-11-28

申请号：US251545

申请日：1999-02-17

Applicant: Edward R. Appelbaum ,  Henry M. Sarau ,  John R. White

Inventor： Edward R. Appelbaum ,  Henry M. Sarau ,  John R. White

IPC: G01N33/68 ,  G01N33/566 ,  C12P21/02 ,  C12P21/06 ,  G01N33/53 ,  G01N33/567

CPC classification number: G01N33/6863 ,  G01N2500/10

Abstract: Disclosed are methods for discovering agonists and antagonists of the interaction between a secreted human protein, chemokine CK.beta.-9, and its cellular receptor, human CCR7, which may have utility in the treatment of several human diseases, including, but not limited to: allergic disorders, autoimmune diseases, ischemia/reperfusion injury, development of atherosclerotic plaques, cancer (including mobilization of hematopoietic stem cells for use in chemotherapy or mycloprotection during chemotherapy), chronic inflammatory disorders, chronic rejection of transplanted organs or tissue grafts, chronic myelogenous leukemia, and infection by HIV and other pathogens.

Abstract translation: 公开了用于发现激动剂和拮抗剂在分泌的人蛋白质趋化因子CKβ-9及其细胞受体人类CCR7之间的相互作用的方法，其可用于治疗几种人类疾病，包括但不限于： 过敏性疾病，自身免疫疾病，缺血/再灌注损伤，动脉粥样硬化斑块发展，癌症（包括动员化学干细胞用于化学疗法或化疗期间的微环境保护），慢性炎性疾病，移植器官或组织移植物的慢性排斥，慢性髓细胞白血病 ，以及艾滋病毒和其他病原体的感染。

公开(公告)号：US4818696A

公开(公告)日：1989-04-04

申请号：US616950

申请日：1984-06-01

Applicant: Edward R. Appelbaum ,  Thomas J. McLoughlin ,  Michael P. O'Connell

Inventor： Edward R. Appelbaum ,  Thomas J. McLoughlin ,  Michael P. O'Connell

IPC: C07K14/195 ,  C12N15/74 ,  C12N15/00 ,  C07H21/00 ,  C12N1/20 ,  C12R1/41

CPC classification number: C12R1/41 ,  C07K14/195 ,  C12N15/743 ,  Y10S435/878

Abstract: A symbiosis plasmid from a fast-growing Rhizobium japonicum donor strain can be transferred to Rhizobium recipient strains, the recipient strains being previously incapable of forming an effective symbiotic relationship with plants of certain Glycine (e.g. soybean) varieties. The recipient strains harboring the symbiosis plasmid will form effective symbioses with soybean plants of said certain varieties, the transferred symbiosis plasmid conferring the host range or specificity of the donor strain on the recipient strains. Methods, strains, and plasmids useful for practice of this invention are also provided.

Abstract translation: 来自快速生长的日本根瘤菌供体菌株的共生质粒可以转移到根瘤菌受体菌株中，受体菌株以前不能与某些甘氨酸（例如大豆）品种的植物形成有效的共生关系。 携带共生质粒的受体菌株将与所述某些品种的大豆植物形成有效的共生，转移的共生质粒赋予受体菌株的供体菌株的宿主范围或特异性。 还提供了可用于实践本发明的方法，菌株和质粒。

公开(公告)号：US07138498B2

公开(公告)日：2006-11-21

申请号：US10646770

申请日：2003-08-25

Applicant: Haodong Li ,  Mark D. Adams ,  Solange H. Gentz ,  Ralph Alderson ,  Yuling Li ,  David Parmelee ,  John R. White ,  Edward R. Appelbaum

Inventor： Haodong Li ,  Mark D. Adams ,  Solange H. Gentz ,  Ralph Alderson ,  Yuling Li ,  David Parmelee ,  John R. White ,  Edward R. Appelbaum

IPC: C07K16/24

CPC classification number: C07K14/52 ,  C07K14/523 ,  Y10S930/14

Abstract: Human chemokine polypeptides and DNA (RNA) encoding such chemokine polypeptides and a procedure for producing such polypeptides by recombinant techniques is disclosed. Also disclosed are methods for utilizing such chemokine polypeptides for the treatment of leukemia, tumors, chronic infections, autoimmune disease, fibrotic disorders, wound healing and psoriasis. Antagonists against such chemokine polypeptides and their use as a therapeutic to treat rheumatoid arthritis, autoimmune and chronic inflammatory and infective diseases, allergic reactions, prostaglandin-independent fever and bone marrow failure are also disclosed.

Abstract translation: 公开了人趋化因子多肽和编码这种趋化因子多肽的DNA（RNA）和通过重组技术产生此类多肽的方法。 还公开了利用这种趋化因子多肽治疗白血病，肿瘤，慢性感染，自身免疫疾病，纤维化病症，伤口愈合和牛皮癣的方法。 还公开了抗这种趋化因子多肽的拮抗剂及其作为治疗类风湿性关节炎，自身免疫性和慢性炎性和感染性疾病，过敏反应，前列腺素依赖性发烧和骨髓衰竭的治疗剂的用途。

公开(公告)号：US06391589B1

公开(公告)日：2002-05-21

申请号：US09479729

申请日：2000-01-07

Applicant: Henrik S. Olsen ,  Haodong Li ,  Mark D. Adams ,  Solange H. L. Gentz ,  Ralph Alderson ,  Yuling Li ,  David Parmelee ,  John R. White ,  Edward R. Appelbaum

Inventor： Henrik S. Olsen ,  Haodong Li ,  Mark D. Adams ,  Solange H. L. Gentz ,  Ralph Alderson ,  Yuling Li ,  David Parmelee ,  John R. White ,  Edward R. Appelbaum

IPC: C12N510

CPC classification number: C07K14/523 ,  A61K38/00 ,  C07K2319/00

Abstract: Human chemokine Beta-10 polypeptides and DNA (RNA) encoding such chemokine polypeptides and a procedure for producing such polypeptides by recombinant techniques is disclosed. Also disclosed are methods for utilizing such chemokine polypeptides for the treatment of leukemia, tumors, chronic infections, autoimmune disease, fibrotic disorders, wound healing and psoriasis. Antagonists against such chemokine polypeptides and their use as a therapeutic to treat rheumatoid arthritis, autoimmune and chronic inflammatory and infective diseases, allergic reactions, prostaglandin-independent fever and bone marrow failure are also disclosed.

Abstract translation: 公开了人趋化因子β-10多肽和编码这种趋化因子多肽的DNA（RNA）和通过重组技术产生这种多肽的方法。 还公开了利用这种趋化因子多肽治疗白血病，肿瘤，慢性感染，自身免疫疾病，纤维化病症，伤口愈合和牛皮癣的方法。 还公开了抗这种趋化因子多肽的拮抗剂及其作为治疗类风湿性关节炎，自身免疫性和慢性炎性和感染性疾病，过敏反应，前列腺素依赖性发烧和骨髓衰竭的治疗剂的用途。

公开(公告)号：US5059534A

公开(公告)日：1991-10-22

申请号：US403907

申请日：1989-07-17

Applicant: Edward R. Appelbaum

Inventor： Edward R. Appelbaum

IPC: C07K14/195 ,  C12N15/74

CPC classification number: C07K14/195 ,  C12N15/743 ,  Y10S435/878

Abstract: The isolation and characterization of nodD-related genes in soybean nodulating Rhizobium japonicum is described. In R. japonicum USDA 191 two such genes have been identified, which although related in structure, have different functional properties. These nodD genes are functionally distinct from each other and from those nodD genes of other strains of Rhizobium that have been isolated and characterized to date. In particular, nodD-r1 has been found to affect nodulation on soybean and to be associated with exopolysaccharide production. In contrast, nodD-r2 affects nodulation on the tropical legume siratro. The coding sequences of both nodD genes are provided. The promoter regions of the nodD genes have also been isolated. The genes described herein, including both structural genes and their promoter regions, can be used in combination with other genetic constructs to enhance the efficiency and competitiveness of nodulation.

Abstract translation: 描述了大豆结核根瘤菌中nodD相关基因的分离和表征。 在日本R.Rapapumum USDA 191中，已经鉴定了两种这样的基因，尽管它们的结构相关，但具有不同的功能特性。 这些nodD基因在功能上是彼此不同的，以及目前已被分离和表征的其他根瘤菌菌株的那些nodD基因。 特别地，已经发现nodD-r1影响大豆的结瘤并与外多糖生产有关。 相比之下，nodD-r2影响热带豆科西罗的结节。 提供了两种nodD基因的编码序列。 nodD基因的启动子区域也已被分离。 本文描述的基因，包括结构基因及其启动子区域，都可以与其他基因构建体结合使用，以提高结瘤的效率和竞争力。

公开(公告)号：US4803165A

公开(公告)日：1989-02-07

申请号：US763800

申请日：1985-08-07

Applicant: Edward R. Appelbaum

Inventor： Edward R. Appelbaum

IPC: C12N15/09 ,  A01K7/00 ,  C07K14/325 ,  C12N1/20 ,  C12N15/52 ,  C12N15/74 ,  C12R1/01 ,  C12R1/19 ,  C12N15/00 ,  C07H15/12 ,  C12P21/00

CPC classification number: C07K14/325 ,  C12N15/52 ,  C12N15/743

Abstract: The promoter of the nifH gene of the fast-growing Rhizobium japonicum strain USDA 191, has been cloned. Over 4.2 kilobase pairs (kbp) of DNA were sequences (FIG. 1). Sequences encoding nifH and the 5'-end of nifD were identified, as were sequences involved in promoting operon transcription and a nifH ribosome binding site. Use of the nifH promoter to drive transcription in Rhizobium of heterologous structural genes is taught. Useful sequences and plasmids are also disclosed.

Abstract translation: 快速生长的日本根瘤菌菌株USDA 191的nifH基因的启动子已被克隆。 超过4.2千碱基对（kbp）的DNA是序列（图1）。 鉴定编码nifH和nifD的5'末端的序列，以及涉及促进操纵子转录和nifH核糖体结合位点的序列。 教导了使用nifH启动子在异源结构基因的根瘤菌中驱动转录。 还公开了有用的序列和质粒。

公开(公告)号：US5229113A

公开(公告)日：1993-07-20

申请号：US393081

申请日：1989-08-09

Applicant: Renee Kosslak ,  Roger Bookland ,  Edward R. Appelbaum

Inventor： Renee Kosslak ,  Roger Bookland ,  Edward R. Appelbaum

IPC: A01C1/00 ,  A01N63/00 ,  C05F11/08 ,  C07K14/195 ,  C12N15/74

CPC classification number: C12N15/74 ,  A01C1/00 ,  A01N63/00 ,  C05F11/08 ,  C07K14/195 ,  Y10S435/878 ,  Y10S47/09 ,  Y10S47/10

Abstract: The chemical compounds that induce expression of nodulation genes of Bradyrhizobium japonicum strains have been identified. The isoflavones daidzein and genistein were identified as soybean exudate components that induce nod-genes. Other isoflavones, including 7-hydroxyisoflavone, 5,7-dihydroxyisoflavone, biochanin A, formononetin and prunetin, were also found to act as inducers. In addition, several flavonoid compounds including 4',7-dihydroxyflavone, apigenin, kaempferol and coumestrol, were also found to act as inducers. Nodulation inducing compositions and a method for selectively activating the expression of a gene under the control of a soybean exudate inducible promoter in B. japonicum in response to these inducer molecules are described. In addition, soybean inoculating compositions containing the nod-gene inducers and inoculating methods employing these compositions are described.

Abstract translation: 已经鉴定了引起日本。Brad of of of of结节基因表达的化合物。 异黄酮黄豆苷原和染料木素被鉴定为诱导结节基因的大豆渗出物成分。 还发现其他异黄酮，包括7-羟基异黄酮，5,7-二羟基异黄酮，鹰嘴豆素A，角叉菜素和杏仁素，作为诱导剂。 此外，还发现了包括4'，7-二羟基黄酮，芹菜素，山奈酚和香豆素的几种类黄酮化合物作为诱导剂。 描述了结瘤诱导组合物和用于选择性地激活在这些诱导剂分子响应于日本粳稻的大豆渗出物诱导型启动子控制下的基因表达的方法。 此外，还描述了含有点状基因诱导剂的大豆接种组合物和使用这些组合物的接种方法。

公开(公告)号：US5023180A

公开(公告)日：1991-06-11

申请号：US61848

申请日：1987-06-11

Applicant: Edward R. Appelbaum ,  Hauke Hennecke ,  Joseph W. Lamb ,  Michael Gottfert

Inventor： Edward R. Appelbaum ,  Hauke Hennecke ,  Joseph W. Lamb ,  Michael Gottfert

IPC: C07K14/195 ,  C12N15/74

CPC classification number: C07K14/195 ,  C12N15/743 ,  C07K2319/00 ,  C07K2319/61 ,  Y10S435/878

Abstract: Nodulation regulatory genes (nodD genes) of Bradyrhizobium japonicum strains have been isolated and sequenced. Recombinant DNA molecules and vectors containing these regulatory genes are described. These genes, molecules and vectors are useful in the genetic engineering of Rhizobium and Bradyrhizobium strains. A method for selective expression of structural genes in response to the application of chemical factors which induce B. japonicum nod genes which employs these genes is described.

Abstract translation: 已经分离并测序了Bradyrhizobium japonicum菌株的结瘤调节基因（nodD基因）。 描述了重组DNA分子和含有这些调节基因的载体。 这些基因，分子和载体可用于根瘤菌和Bradyrhizobium菌株的遗传工程。 描述了应用这些诱导使用这些基因的日本粳稻节点基因的化学因子的应用来选择性表达结构基因的方法。