公开(公告)号：US20110034541A1

公开(公告)日：2011-02-10

申请号：US12697917

申请日：2010-02-01

申请人： Eyal Raz ,  Mark Roman

发明人： Eyal Raz ,  Mark Roman

IPC分类号： A61K48/00 ,  C07H21/00 ,  C12N15/85 ,  C40B30/06 ,  C12Q1/68

CPC分类号： C07H21/00 ,  A61K38/00 ,  A61K39/39 ,  A61K2039/55561 ,  A61K2039/57 ,  C07K14/4703 ,  C12N15/117 ,  C12N2310/111 ,  C12N2310/17 ,  C12N2310/18 ,  C12N2310/315 ,  C12N2310/33 ,  C12N2310/334 ,  C12N2310/3513 ,  C12Q1/6883 ,  Y02A50/41 ,  Y02A50/423

摘要： The invention consists of oligonucleotides which inhibit the immunostimulatory activity of ISS-ODN (immunostimulatory sequence oligodeoxynucleotides) as well as methods for their identification and use. The oligonucleotides of the invention are useful in controlling therapeutically intended ISS-ODN adjuvant activity as well as undesired ISS-ODN activity exerted by recombinant expression vectors, such as those used for gene therapy and gene immunization. The oligonucleotides of the invention also have anti-inflammatory activity useful in reducing inflammation in response to infection of a host with ISS-ODN containing microbes, in controlling autoimmune disease and in boosting host Th2 type immune responses to an antigen. The invention also encompasses pharmaecutically useful conjugates of the oligonucleotides of the invention (including conjugate partners such as antigens and antibodies).

摘要翻译： 本发明由抑制ISS-ODN（免疫刺激性寡脱氧核苷酸）的免疫刺激活性的寡核苷酸以及鉴定和使用的方法组成。 本发明的寡核苷酸可用于控制治疗性意图的ISS-ODN佐剂活性以及重组表达载体（例如用于基因治疗和基因免疫的那些）所产生的不期望的ISS-ODN活性。 本发明的寡核苷酸还具有抗炎活性，其可用于在控制具有含有ISS-ODN的微生物的宿主的感染中，在控制自身免疫疾病和促进对抗原的宿主Th2型免疫应答中减少炎症。 本发明还包括本发明的寡核苷酸的药物有用的缀合物（包括缀合物如抗原和抗体）。

公开(公告)号：US20050175630A1

公开(公告)日：2005-08-11

申请号：US11021821

申请日：2004-12-22

申请人： Eyal Raz ,  Joshua Fierer

发明人： Eyal Raz ,  Joshua Fierer

IPC分类号： A61K39/116 ,  A61K39/39 ,  A61K39/02 ,  A61K39/108 ,  A61K39/04 ,  A61K39/095 ,  A61K39/112

CPC分类号： A61K39/39 ,  A61K39/0208 ,  A61K39/0275 ,  A61K39/07 ,  A61K2039/521 ,  A61K2039/541 ,  A61K2039/542 ,  A61K2039/55594 ,  A61K2039/70 ,  Y02A50/47 ,  Y02A50/482

摘要： The present invention provides an immunogenic composition comprising lethally irradiated bacteria formulated for mucosal delivery. The present invention further provides methods of preparing a subject immunogenic composition. The present invention further provides a method of inducing an immune response in an individual to an antigen, the method generally involving administering a subject immunogenic composition to a mucosal tissue of the individual.

摘要翻译： 本发明提供一种免疫原性组合物，其包含配制用于粘膜递送的致死辐照细菌。 本发明还提供了制备受试者免疫原性组合物的方法。 本发明还提供了诱导个体对抗原的免疫应答的方法，该方法通常涉及将个体免疫原性组合物给予个体的粘膜组织。

公开(公告)号：US5804566A

公开(公告)日：1998-09-08

申请号：US333068

申请日：1994-11-01

申请人： Dennis A. Carson ,  Eyal Raz ,  Meredith L. Howell

发明人： Dennis A. Carson ,  Eyal Raz ,  Meredith L. Howell

IPC分类号： C12N15/09 ,  A61B17/20 ,  A61K9/00 ,  A61K9/08 ,  A61K9/12 ,  A61K31/70 ,  A61K31/7088 ,  A61K38/00 ,  A61K38/22 ,  A61K39/00 ,  A61K39/35 ,  A61K48/00 ,  A61N1/30 ,  A61P37/04 ,  A61P43/00 ,  C07K14/11 ,  C07K14/495 ,  C07K14/54 ,  C07K14/55 ,  C07K14/77 ,  C07K16/00 ,  C12N5/10 ,  C12N15/12 ,  C12N15/87 ,  G01N33/50 ,  G01N33/569 ,  G01N33/68 ,  C12N15/52

CPC分类号： C07K14/005 ,  A61B17/20 ,  A61B17/205 ,  A61K31/711 ,  A61K39/00 ,  A61K39/0005 ,  A61K39/0011 ,  A61K39/35 ,  A61K48/00 ,  C07K14/495 ,  C07K14/5406 ,  C07K14/55 ,  C07K14/77 ,  C07K16/00 ,  C12N15/87 ,  G01N33/5091 ,  G01N33/56977 ,  G01N33/6863 ,  G01N33/6872 ,  A61K2039/51 ,  A61K2039/5154 ,  A61K2039/53 ,  A61K2039/541 ,  A61K38/00 ,  C07K2317/77 ,  C12N2760/16022 ,  C12N2760/16122

摘要： The invention is directed to methods for introducing biologically active peptides into a host by administration of polynucleotides which operatively encode for the peptide of interest. In a preferred embodiment of the invention, a mammal is desensitized to an antigen, in particular an allergen, through administration to the mammal of polynucleotides which operatively encode the antigen. The antigen-encoding polynucleotides are administered to host tissues which have a high concentration of antigen presenting cells in them relative to other host tissues. The method is particularly useful in treating allergies because the allergen-encoding polynucleotides of the invention to induce tolerance while suppressing IgE antibody formation. Devices and compositions for use in the methods of the invention are also described.

摘要翻译： 本发明涉及通过施用有效编码目的肽的多核苷酸将生物活性肽引入宿主的方法。 在本发明的优选实施方案中，通过向哺乳动物施用有效编码抗原的多核苷酸，使哺乳动物脱敏至抗原，特别是变应原。 将抗原编码多核苷酸相对于其他宿主组织施用于其中具有高浓度抗原呈递细胞的宿主组织。 该方法特别可用于治疗过敏，因为本发明的变应原编码多核苷酸在抑制IgE抗体形成的同时诱导耐受。 还描述了用于本发明方法的装置和组合物。

公开(公告)号：US5679647A

公开(公告)日：1997-10-21

申请号：US334260

申请日：1994-11-03

申请人： Dennis A. Carson ,  Eyal Raz

发明人： Dennis A. Carson ,  Eyal Raz

IPC分类号： C12N15/09 ,  A61B17/20 ,  A61K9/00 ,  A61K9/08 ,  A61K9/12 ,  A61K31/07 ,  A61K31/56 ,  A61K31/59 ,  A61K31/70 ,  A61K31/7088 ,  A61K38/00 ,  A61K38/22 ,  A61K39/00 ,  A61K39/35 ,  A61K48/00 ,  A61N1/30 ,  A61P35/00 ,  A61P37/00 ,  A61P37/04 ,  A61P43/00 ,  C07K14/11 ,  C07K14/495 ,  C07K14/54 ,  C07K14/55 ,  C07K14/705 ,  C07K14/77 ,  C07K16/00 ,  C12N5/10 ,  C12N15/00 ,  C12N15/12 ,  C12N15/87 ,  C12R1/91 ,  G01N33/50 ,  G01N33/569 ,  G01N33/574 ,  G01N33/68 ,  C12N15/52

CPC分类号： A61K31/711 ,  A61B17/20 ,  A61B17/205 ,  A61K39/00 ,  A61K39/0005 ,  A61K39/0011 ,  A61K39/35 ,  A61K48/00 ,  C07K14/005 ,  C07K14/495 ,  C07K14/5406 ,  C07K14/55 ,  C07K14/77 ,  C07K16/00 ,  C12N15/87 ,  G01N33/5091 ,  G01N33/56977 ,  G01N33/6863 ,  G01N33/6872 ,  A61K2039/51 ,  A61K2039/5154 ,  A61K2039/53 ,  A61K2039/541 ,  A61K38/00 ,  C07K2317/77 ,  C12N2760/16022

摘要： The invention is directed to methods for introducing biologically active peptides into a host by administration of polynucleotides which operatively encode for the peptide of interest. In a preferred embodiment of the invention, a host who has been identified as having a tumor bearing at least one tumor-associated antigen is the recipient of a polynucleotide which operatively encodes for a foreign mimic of the tumor-associated antigen or a mutation of the self-antigen. The antigen-encoding polynucleotides are administered to host tissues which have a high concentration of antigen presenting cells in them relative to other host tissues. The method is particularly useful in treating cancer through induction of antigen-specific cytotoxic T lymphocytes in the host for lysis of tumor cells bearing the antigen. Devices and compositions for use in the methods of the invention are also described.

摘要翻译： 本发明涉及通过施用有效编码目的肽的多核苷酸将生物活性肽引入宿主的方法。 在本发明的优选实施方案中，被鉴定为具有至少一种肿瘤相关抗原的肿瘤的宿主是可操作地编码肿瘤相关抗原的外源模拟物的多核苷酸的受体或 自身抗原。 将抗原编码多核苷酸相对于其他宿主组织施用于其中具有高浓度抗原呈递细胞的宿主组织。 该方法通过在宿主中诱导具有抗原的肿瘤细胞裂解的抗原特异性细胞毒性T淋巴细胞来治疗癌症特别有用。 还描述了用于本发明方法的装置和组合物。

公开(公告)号：US07560436B2

公开(公告)日：2009-07-14

申请号：US11359945

申请日：2006-02-21

申请人： Eyal Raz ,  Kyoko Katakura ,  Jongdae Lee ,  Daniel Rachmilewitz

发明人： Eyal Raz ,  Kyoko Katakura ,  Jongdae Lee ,  Daniel Rachmilewitz

IPC分类号： A01N43/04 ,  A61K31/70

CPC分类号： A61K31/70 ,  A61K31/4745 ,  A61K31/5377 ,  A61K31/7056 ,  A61K31/7072 ,  A61K31/7076 ,  A61K31/7088

摘要： The present invention provides methods of treating gastrointestinal inflammation, methods of treating inflammatory bowel disease, methods of treating Crohn's Disease, and methods of treating ulcerative colitis in an individual. The methods generally involve administering an effective amount of an agent that increases the level of a Type I interferon and/or that activates a Type I interferon signaling pathway in the individual.

摘要翻译： 本发明提供治疗胃肠道炎症的方法，治疗炎症性肠病的方法，治疗克罗恩病的方法和治疗个体溃疡性结肠炎的方法。 所述方法通常包括施用有效量的增加I型干扰素水平的试剂和/或激活个体中的I型干扰素信号通路。

公开(公告)号：US07485627B2

公开(公告)日：2009-02-03

申请号：US10412151

申请日：2003-04-11

申请人： Eyal Raz ,  Daniel Rachmilewitz

发明人： Eyal Raz ,  Daniel Rachmilewitz

IPC分类号： A61K31/70

CPC分类号： A61K31/7088 ,  A61K31/7105 ,  A61K31/711 ,  A61K47/6893 ,  A61K47/6897 ,  A61K47/6898 ,  B82Y5/00

摘要： The invention provides a method for ameliorating gastrointestinal inflammation, particularly chronic gastrointestinal inflammation such as inflammatory bowel disease (IBD), in a subject. In one embodiment, the method comprises administering an immunomodulatory nucleic acid to a subject suffering from or susceptible to gastrointestinal inflammation.

公开(公告)号：US06893821B2

公开(公告)日：2005-05-17

申请号：US09848986

申请日：2001-05-04

申请人： Eyal Raz ,  Augusto Lois ,  Kenji Takabayashi

发明人： Eyal Raz ,  Augusto Lois ,  Kenji Takabayashi

IPC分类号： A61K31/7088 ,  C12Q1/48 ,  G01N33/68 ,  C12Q1/68 ,  A61K38/00 ,  C07H21/00 ,  C07H21/04 ,  C07K1/00 ,  C12N1/20 ,  C12N15/00 ,  C12N15/63

CPC分类号： A61K31/7088 ,  C12Q1/485 ,  G01N33/6869

摘要： The present invention provides methods for modulating cell death in a eukaryotic cell, and methods for reducing DNA damage in a eukaryotic cell. The methods generally comprise modulating a biological activity of DNA-PK in a cell. The invention further provides methods of treating a condition related to cell death in an individual. The invention further provides methods of identifying agents which modulate a biological activity of DNA-PK, as well as agents identified by the methods. Methods of modulating an immune response using an identified agent are also provided.

摘要翻译： 本发明提供了调节真核细胞中细胞死亡的方法，以及用于减少真核细胞中的DNA损伤的方法。 所述方法通常包括调节细胞中DNA-PK的生物活性。 本发明还提供了治疗个体中与细胞死亡有关的病症的方法。 本发明还提供鉴定调节DNA-PK的生物活性的试剂以及通过该方法鉴定的试剂的方法。 还提供了使用鉴定的药剂调节免疫应答的方法。

公开(公告)号：US06613751B2

公开(公告)日：2003-09-02

申请号：US09791500

申请日：2001-02-22

申请人： Eyal Raz ,  Daniel Rachmilewitz

发明人： Eyal Raz ,  Daniel Rachmilewitz

IPC分类号： A61K3170

CPC分类号： A61K31/7088 ,  A61K31/7105 ,  A61K31/711 ,  A61K47/6893 ,  A61K47/6897 ,  A61K47/6898 ,  B82Y5/00

摘要： The invention provides a method for ameliorating gastrointestinal inflammation, particularly chronic gastrointestinal inflammation such as inflammatory bowel disease (IBD), in a subject. In one embodiment, the method comprises administering an immunomodulatory nucleic acid to a subject suffering from or susceptible to gastrointestinal inflammation.

公开(公告)号：US20110182927A1

公开(公告)日：2011-07-28

申请号：US12862429

申请日：2010-08-24

申请人： EYAL RAZ ,  MARK ROMAN

发明人： EYAL RAZ ,  MARK ROMAN

IPC分类号： A61K48/00 ,  A61K39/00 ,  A61P37/02

CPC分类号： C07H21/00 ,  A61K38/00 ,  A61K39/39 ,  A61K2039/55561 ,  A61K2039/57 ,  C07K14/4703 ,  C12N15/117 ,  C12N2310/111 ,  C12N2310/17 ,  C12N2310/18 ,  C12N2310/315 ,  C12N2310/33 ,  C12N2310/334 ,  C12N2310/3513 ,  C12Q1/6883 ,  Y02A50/41 ,  Y02A50/423

摘要： The invention consists of oligonucleotides which inhibit the immunostimulatory activity of ISS-ODN (immunostimulatory sequence oligodeoxynucleotides) as well as methods for their identification and use. The oligonucleotides of the invention are useful in controlling therapeutically intended ISS-ODN adjuvant activity as well as undesired ISS-ODN activity exerted by recombinant expression vectors, such as those used for gene therapy and gene immunization. The oligonucleotides of the invention also have anti-inflammatory activity useful in reducing inflammation in response to infection of a host with ISS-ODN containing microbes, in controlling autoimmune disease and in boosting host Th2 type immune responses to an antigen. The invention also encompasses pharmaceutically useful conjugates of the oligonucleotides of the invention (including conjugate partners such as antigens and antibodies).

摘要翻译： 本发明由抑制ISS-ODN（免疫刺激性寡脱氧核苷酸）的免疫刺激活性的寡核苷酸以及鉴定和使用的方法组成。 本发明的寡核苷酸可用于控制治疗性意图的ISS-ODN佐剂活性以及重组表达载体（例如用于基因治疗和基因免疫的那些）所产生的不期望的ISS-ODN活性。 本发明的寡核苷酸还具有抗炎活性，其可用于在控制具有含有ISS-ODN的微生物的宿主的感染中，在控制自身免疫疾病和促进对抗原的宿主Th2型免疫应答中减少炎症。 本发明还包括本发明的寡核苷酸的药学上有用的缀合物（包括缀合物如抗原和抗体）。

公开(公告)号：US20080008741A1

公开(公告)日：2008-01-10

申请号：US10588573

申请日：2005-01-27

申请人： Eyal Raz ,  Daniel Rachmilewitz

发明人： Eyal Raz ,  Daniel Rachmilewitz

IPC分类号： A61K31/7088 ,  A61K47/00

CPC分类号： A61K31/7088

摘要： The present invention provides methods of treating irritable bowel syndrome (IBS) in an individual suffering from IBS. The methods generally involve administering to the individual an effective amount of a therapeutic nucleic acid. The invention further provides kits and compositions for practicing the subject methods.

摘要翻译： 本发明提供了在患有IBS的个体中治疗肠易激综合征（IBS）的方法。 所述方法通常涉及向个体施用有效量的治疗性核酸。 本发明还提供了用于实践本发明方法的试剂盒和组合物。