公开(公告)号：US09072311B2

公开(公告)日：2015-07-07

申请号：US10530598

申请日：2004-06-21

Applicant: Moti Harel ,  John Piechocki ,  David J. Kyle

Inventor： Moti Harel ,  John Piechocki ,  David J. Kyle

IPC: A23K1/18 ,  A23K1/16 ,  A23K1/00 ,  A23K1/175 ,  A61K31/065 ,  A61K31/66

CPC classification number: A61K31/685 ,  A23K20/158 ,  A23K20/174 ,  A23K20/179 ,  A23K20/22 ,  A23K20/26 ,  A23K40/30 ,  A23K50/75 ,  A23K50/80 ,  A61K31/065 ,  A61K31/66 ,  A61K45/06 ,  A61K2300/00

Abstract: Carotenoids are provided as dietary supplements to animal feed. These supplements improve the bioavailability of carotenoids by providing them in combination with phospholipids. The invention provides animal feeds for aquatic and terrestrial animals, and methods for making the feeds.

Abstract translation: 提供类胡萝卜素作为动物饲料的膳食补充剂。 这些补充剂通过将其与磷脂组合提供来提高类胡萝卜素的生物利用度。 本发明提供了用于水生和陆生动物的动物饲料以及用于制备饲料的方法。

公开(公告)号：US6027900A

公开(公告)日：2000-02-22

申请号：US155972

申请日：1998-10-09

Applicant: F. C. Thomas Allnutt ,  David J. Kyle ,  Arthur R. Grossman ,  Kirk R. Apt

Inventor： F. C. Thomas Allnutt ,  David J. Kyle ,  Arthur R. Grossman ,  Kirk R. Apt

IPC: C12N15/65 ,  C12N15/79 ,  C12Q1/68

CPC classification number: C12N15/79 ,  C12N15/65

Abstract: Genetic fusions for use in genetic engineering of eukaryotic algae employ a promoter from a light harvesting protein fused to a protein of interest. The fusions can be introduced and selected using an antibiotic resistance determinant. One gene useful for such selection is the sh ble gene encoding a bleomycin binding protein.

Abstract translation: PCT No.PCT / US97 / 06021 Sec。 371日期：1998年10月9日 102（e）日期1998年10月9日PCT 1997年4月11日PCT PCT。 WO97 / 39106 PCT公开号 日期1997年10月23日用于真核藻类遗传工程的遗传融合使用与感兴趣的蛋白质融合的光收获蛋白质的启动子。 可以使用抗生素抗性决定簇引入和选择融合体。 用于这种选择的一个基因是编码博来霉素结合蛋白的sh ble基因。

公开(公告)号：US5376540A

公开(公告)日：1994-12-27

申请号：US936248

申请日：1992-09-24

Applicant: David J. Kyle

Inventor： David J. Kyle

IPC: A61K49/00 ,  A61K51/12 ,  C07C57/12 ,  C11B1/00 ,  C11B1/10 ,  C12N1/06 ,  C12P7/62 ,  C12P7/64 ,  C12R1/89 ,  G01N33/497 ,  G01N33/60

CPC classification number: A61K51/1206 ,  C11B1/00 ,  C12N1/06 ,  C12P7/6463 ,  C12P7/6472 ,  G01N33/497 ,  A61K2123/00 ,  Y10S435/946

Abstract: Labelled triglyceride oils are produced for use in diagnostic breath tests. The process involves the cultivation of microorganisms capable of producing the oils in an appropriately controlled environment wherein the microorganisms are feed .sup.13 C or .sup.14 C labeled carbon substrates to induce the microorganisms to produce the labeled oils. The preferred labelled carbon substrate is a substrate containing .sup.13 C. The oil produced is preferrably enriched in oleic acid. The microorganisms can be induced to produce the oil by nitrogen depletion during cultivation.

Abstract translation: 生产标记的甘油三酯油用于诊断性呼气试验。 该方法包括培养能够在适当控制的环境中生产油的微生物，其中微生物进料13C或14C标记的碳底物以诱导微生物产生标记的油。 优选的标记碳底物是含有13℃的底物。 产生的油优选地富含油酸。 在培养过程中，微生物可通过氮耗竭诱导产生油。

公开(公告)号：US5244921A

公开(公告)日：1993-09-14

申请号：US496572

申请日：1990-03-21

Applicant: David J. Kyle ,  Raymond Gladue

Inventor： David J. Kyle ,  Raymond Gladue

IPC: A23D9/00 ,  A23K1/00 ,  A23K1/16 ,  A23K1/18 ,  A23L1/30 ,  A61K8/30 ,  A61K8/36 ,  A61K8/37 ,  A61K8/96 ,  A61K8/97 ,  A61K8/99 ,  A61K31/20 ,  A61K31/23 ,  A61K36/02 ,  A61P17/00 ,  C07C57/03 ,  C11B1/00 ,  C12N1/12 ,  C12P7/64 ,  C12R1/89

CPC classification number: C12P7/6472 ,  A23K20/158 ,  A23K50/80 ,  C07C57/03 ,  C12N1/12

Abstract: This invention describes a process for producing single cell edible oils containing eicosapentaenoic acid (EPA) from heterotrophic diatoms. The diatoms are cultivated in a fermentor in a nutrient solution containing nitrogen and silicate. Withdrawal of the nitrogen followed by withdrawal of the silicate induces the diatoms to synthesize large quantities of edible oil containing EPA which subsequently is recovered. The edible oil, and uses for it, also form parts of this invention as do mutant diatoms capable of producing large quantities of EPA.

Abstract translation: 本发明描述了从异养硅藻生产含有二十碳五烯酸（EPA）的单细胞食用油的方法。 硅藻在含有氮和硅酸盐的营养液中的发酵罐中培养。 退出氮气后，硅酸盐的抽出物会诱导硅藻合成大量含EPA的食用油，随后将其回收。 食用油及其用途也与能够产生大量EPA的突变硅藻一样形成本发明的一部分。

公开(公告)号：US20120034653A1

公开(公告)日：2012-02-09

申请号：US13188806

申请日：2011-07-22

Applicant: Kirk Emil APT ,  F.C. Thomas Allnutt ,  David J. Kyle ,  James Casey Lippmeier

Inventor： Kirk Emil APT ,  F.C. Thomas Allnutt ,  David J. Kyle ,  James Casey Lippmeier

IPC: C12P21/00 ,  C12N15/79 ,  C12N1/13 ,  C07C29/74 ,  C11B1/10 ,  C07C35/21 ,  C07C7/00 ,  C07K14/405 ,  A23K1/00 ,  C07K1/14

CPC classification number: C12N15/8209 ,  C12N1/12 ,  C12N15/65 ,  C12N15/79 ,  C12N15/8207 ,  Y10S435/946

Abstract: Most microalgae are obligate photoautotrophs and their growth is strictly dependent on the generation of photosynthetically-derived energy. In this study it is shown that the microalga Phaeodaclylurn tricornutum can be engineered to import glucose and grow in the dark through the introduction of genes encoding glucose transporters. Both the human and Chlorella kessleri glucose transporters facilitated the uptake of glucose by P. tricornutum, allowing the cells to metabolize exogenous organic carbon and thrive, independent of light. This is the first successful trophic conversion of an obligate photoautotroph through metabolic engineering, and it demonstrates that methods of cell nourishment can be fundamentally altered with the introduction of a single gene. Since strains transformed with the glucose transport genes are able to grow non-photosynthetically, they can be exploited for the analysis of photosynthetic processes through mutant generation and characterization. Finally, this work also represents critical progress toward large-scale commercial exploitation of obligate phototrophic algae through the use of microbial fermentation technology, eliminating significant limitations resulting from light-dependent growth.

Abstract translation: 大多数微藻是专性光自养体，其生长严格依赖于光合作用衍生能量的产生。 在这项研究中，显示微藻Phaeodaclylurn三角褐豆可以通过引入编码葡萄糖转运蛋白的基因进行工程化以导入葡萄糖并在黑暗中生长。 人和小球藻凯斯勒葡萄糖转运蛋白都促进了三角褐指藻的葡萄糖摄取，使得细胞代谢外源有机碳，并且独立于光。 这是通过代谢工程首次成功地营养专一性自养型营养转化，并且表明通过引入单一基因可以从根本上改变细胞营养的方法。 由于用葡萄糖转运基因转化的菌株能够非光合作用生长，因此可以通过突变体的产生和表征来开发光合作用的分析。 最后，这项工作也是通过使用微生物发酵技术大规模商业开发专用光营养藻类的重要进展，消除了光依赖性生长造成的重大限制。

公开(公告)号：US07932056B2

公开(公告)日：2011-04-26

申请号：US12489905

申请日：2009-06-23

Applicant: Ruth Barratt ,  F. C. Thomas Allnutt ,  Robert Bullis ,  David J. Kyle

Inventor： Ruth Barratt ,  F. C. Thomas Allnutt ,  Robert Bullis ,  David J. Kyle

IPC: C12P21/00 ,  C12N5/06 ,  C12N7/00

CPC classification number: C07K14/005 ,  A01K2227/70 ,  A01K2267/01 ,  A61K2039/5256 ,  C07K14/505 ,  C07K14/62 ,  C07K16/00 ,  C07K2317/10 ,  C12N7/00 ,  C12N9/16 ,  C12N15/86 ,  C12N2710/14043 ,  C12N2710/18043 ,  C12N2750/14043 ,  C12N2770/16022 ,  C12N2770/16034 ,  C12N2770/22043 ,  C12N2770/36143 ,  C12N2830/00 ,  C12N2830/75

Abstract: A crustacean or rotifer is infected with a recombinant infectious virus that expresses a protein exogenous to the virus. The genome of the crustacean or rotifer itself remains unaltered. Crustacean, rotifer, insect, or viral promoters drive the transcription of a gene inserted into the recombinant virus genome, and the virus replicates in the crustacean or rotifer cell cytoplasm. The infected crustacean or rotifer can be provided directly to humans or non-human animals, or, following production and harvest of the crustaceans or rotifers, purified recombinant protein or polypeptide can be provided. Large quantities of biopharmaceuticals can be produced rapidly and inexpensively using this production system.

Abstract translation: 用表达外源蛋白质的病毒的重组感染性病毒感染甲壳类动物或轮虫。 甲壳类或轮虫本身的基因组本身保持不变。 甲壳动物，轮虫，昆虫或病毒启动子驱动插入重组病毒基因组中的基因的转录，并且病毒在甲壳类或轮状细胞细胞质中复制。 受感染的甲壳动物或轮虫可以直接提供给人类或非人动物，或者在甲壳类动物或轮虫的生产和收获后，可提供纯化的重组蛋白或多肽。 可以使用该生产系统快速且廉价地生产大量的生物药物。

公开(公告)号：US20090238890A1

公开(公告)日：2009-09-24

申请号：US12160497

申请日：2007-01-16

Applicant: John Piechocki ,  David J. Kyle

Inventor： John Piechocki ,  David J. Kyle

IPC: A61K9/14

CPC classification number: C08J3/03 ,  C08B37/0084 ,  C08J3/075 ,  C08J3/122 ,  C08J3/24 ,  C08J2303/02 ,  C08J2305/04 ,  C08L3/02 ,  C08L5/04

Abstract: The disclosure relates to novel microencapsulation processes based on the use of high viscosity fluids (e.g., gelatinized starch and alginate), which are mixed and then sprayed using a much gentler hydraulic pressure and, preferably gas-based atomization into a crosslinking solution (e.g. of calcium chloride). To improve the efficiency of the system, the process can be performed in a continuous mode rather than by a conventional batch process. This involves continuous or intermittent harvest of the microparticles collected in the capture vessel followed by amendment and recycling of the CaCl2 solution and its redeployment into the capture vessel. The process allows production of microencapsulated probiotic bacteria without major losses in viability, thereby providing a useful and efficient new manufacturing method for the stabilization of probiotic bacteria prior to their introduction into functional foods.

Abstract translation: 本公开涉及基于使用高粘度流体（例如糊化淀粉和藻酸盐）的新型微胶囊化方法，所述新型微胶囊化方法被混合并且然后使用较轻的液压进行喷雾，并且优选基于气体雾化进入交联溶液（例如 氯化钙）。 为了提高系统的效率，该过程可以以连续模式而不是常规的批处理进行。 这涉及收集在捕获容器中的微粒的连续或间歇收获，随后修改和回收CaCl 2溶液并将其重新部署到捕获容器中。 该方法允许生产微胶囊化的益生菌，而不会在生存力方面造成重大损失，从而为在益生菌引入功能性食品之前稳定益生菌提供了有用和高效的新的制造方法。

公开(公告)号：US5550156A

公开(公告)日：1996-08-27

申请号：US358474

申请日：1994-12-19

Applicant: David J. Kyle

Inventor： David J. Kyle

IPC: A23C9/152 ,  A23C9/20 ,  A23C11/02 ,  A23D9/007 ,  A23L1/29 ,  A23L1/30 ,  A61K31/20 ,  A61K31/23 ,  A61P3/00 ,  C12P7/64 ,  A61K31/225

CPC classification number: A61K31/23 ,  A23L33/12 ,  A23L33/40 ,  A61K31/20 ,  A23V2002/00

Abstract: The present invention relates to compositions including blends of microbial oils, methods of using such compositions, particularly as supplements for infant formula, and methods of increasing the amount of long chain polyunsaturated fatty acids in infant formula.

Abstract translation: 本发明涉及组合物，包括微生物油的混合物，使用这些组合物的方法，特别是作为婴儿配方食品的补充剂，以及增加婴儿配方中长链多不饱和脂肪酸的量的方法。

公开(公告)号：US5492938A

公开(公告)日：1996-02-20

申请号：US386079

申请日：1995-02-09

Applicant: David J. Kyle ,  Sue E. Reeb ,  Valerie J. Sicotte

Inventor： David J. Kyle ,  Sue E. Reeb ,  Valerie J. Sicotte

IPC: A23K1/18 ,  A23C9/152 ,  A23D9/00 ,  A23D9/007 ,  A23D9/02 ,  A23K1/00 ,  A23K1/16 ,  A23L1/30 ,  A61K9/48 ,  A61K31/20 ,  A61K31/202 ,  A61K31/23 ,  A61K35/60 ,  A61K36/02 ,  A61K47/14 ,  A61K47/44 ,  A61P3/00 ,  A61P3/02 ,  A61P9/00 ,  A61P9/10 ,  C11B1/10 ,  C11C3/00 ,  C12N1/12 ,  C12N1/13 ,  C12N15/09 ,  C12P1/00 ,  C12P7/64 ,  C12R1/89 ,  C12R1/90 ,  C12P7/40

CPC classification number: C12P7/6472 ,  A23D9/00 ,  A23K20/158 ,  A23K50/80 ,  A23L33/115 ,  C11B1/10 ,  C12R1/89 ,  Y10S426/801 ,  Y10S426/805 ,  Y10S435/946

Abstract: A pharmaceutical composition and a dietary supplement comprising a single cell oil containing at least 20% docosahexaenoic acid (DHA) in triglyceride form are presented. The single cell oil is extracted from biomass produced by cultivating heterotrophic microalgae of the class Dinophyceae in an aerobic fermentor with a nutrient solution having a limited nitrogen source.

Abstract translation: 提供了包含含有甘油三酯形式的至少20％二十二碳六烯酸（DHA）的单细胞油的药物组合物和膳食补充剂。 通过在具有有限氮源的营养液的需氧发酵罐中培养Dinophyceae类的异养微藻产生的生物质提取单细胞油。

公开(公告)号：US08198067B2

公开(公告)日：2012-06-12

申请号：US11449829

申请日：2006-06-09

Applicant: David J. Kyle

Inventor： David J. Kyle

IPC: C12N1/14 ,  A61K38/00 ,  A23K1/17

CPC classification number: A61K35/745 ,  A23K10/10 ,  A23K10/18 ,  A23K20/147 ,  A23K50/80 ,  A23L17/60 ,  A61K35/742 ,  A61K35/747 ,  A61K36/02 ,  A61K36/06

Abstract: A microbial biomass, made from algae, bacteria, fungi, yeast, or combinations thereof, provides a feed for animals raised either in agriculture or aquaculture. A feed additive, and a therapeutic composition can also be made from a microbial biomass of algae, bacteria, fungi, yeast, or combinations thereof. The feed, feed additive, and therapeutic composition can comprise one or more proteins, peptides, antibodies, antibody fragments, or a combination thereof, wherein said proteins, peptides, antibodies, antibody fragments, or a combination thereof are non-native to the microbes of the biomass. The biomass can have therapeutic, bioactive, nutritional, and/or immunogenic properties.

Abstract translation: 由藻类，细菌，真菌，酵母或其组合制成的微生物生物质为农业或水产养殖中提出的动物提供饲料。 饲料添加剂和治疗组合物也可以由藻类，细菌，真菌，酵母或其组合的微生物生物质制成。 饲料，饲料添加剂和治疗组合物可以包含一种或多种蛋白质，肽，抗体，抗体片段或其组合，其中所述蛋白质，肽，抗体，抗体片段或其组合对微生物是非天然的 的生物量。 生物质可以具有治疗，生物活性，营养和/或免疫原性。