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1.发明授权Cloning of the gene which codes for the pilinic subunit fim3 of Bordetella pertussis 失效克隆编码百日咳百日咳杆菌的pilinic亚基fim3的基因
公开(公告)号：US5525489A
公开(公告)日：1996-06-11
申请号：US29952
申请日：1993-03-09
CPC分类号： C07K14/235 , A61K39/00
摘要： A cloned DNA fragment of Bordetella pertussis including the gene which codes for the pilinic subunit fim3, vectors which contain it and microorganisms transformed by the vectors.The protein and peptides corresponding to at least one epitope of the gene which codes for the pilinic subunit fim3 are particularly useful for the development of acellular anti-pertussis vaccines.In addition, a strain of Bordetella modified by a recombinant replication or genome-integration vector containing the cloned DNA fragment or the gene or a fraction thereof is particularly suitable for the development of a cellular anti-pertussis vaccine.
摘要翻译： 百日咳博德特氏菌的克隆DNA片段，包括编码pilinic亚基fim3的基因，含有载体的载体和载体转化的微生物。 对应于编码pilinic亚基fim3的基因的至少一个表位的蛋白质和肽特别可用于开发无细胞抗百日咳疫苗。 此外，通过含有克隆的DNA片段或其基因或其部分的重组复制或基因组整合载体修饰的博德特氏菌菌株特别适用于细胞抗百日咳疫苗的开发。
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公开(公告)号：US09085759B2
公开(公告)日：2015-07-21
申请号：US12747990
申请日：2008-12-03
申请人： Giacomo Rispoli , Emiliano Fioravanti , Renzo Bignazzi , Ezio Nicola D'Addario , Francesca De Ferra , Federico Capuano
发明人： Giacomo Rispoli , Emiliano Fioravanti , Renzo Bignazzi , Ezio Nicola D'Addario , Francesca De Ferra , Federico Capuano
CPC分类号： C12N1/12 , C12M21/02 , C12M23/18 , C12M23/58 , C12M29/02 , C12M43/04 , C12M47/02 , C12M47/06
摘要： The invention relates to a process for the production of algal biomass with a high lipid content, comprising: (a) the production of inocula in order to effect phase (b), in photoreactors; (b) the massive cultivation of the algal biomass in open ponds, inoculated with phase (a); (c) a thickening phase of the algal biomass, effected blandly; (d) an induction phase of the lipid production, wherein modules are used consisting of photoreactors or open ponds; (e) a separation phase of the biomass with a high lipid content.
摘要翻译： 本发明涉及一种生产具有高脂质含量的藻类生物质的方法，包括：（a）在光反应器中生产接种物以实现阶段（b）; （b）接种阶段（a）的露天池塘中大量培养藻类生物量; （c）藻类生物质的增稠期，平缓地进行; （d）脂质生产的诱导期，其中使用由光反应器或露天池组成的模块; （e）具有高脂质含量的生物质的分离阶段。
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3.发明申请公开(公告)号：US20080311577A1
公开(公告)日：2008-12-18
申请号：US11995042
申请日：2006-07-07
摘要： A method is described for the identification of sulfooxidizing bacteria comprising the extraction of the DNA from environmental samples and the subsequent identification of at least one fragment of the Thio 16S gene or SoxB gene present in these bacteria. The method can be used for determining the level of elemental sulfur in samples of soil.
摘要翻译： 描述了一种用于鉴定磺化氧化细菌的方法，其包括从环境样品中提取DNA，随后鉴定存在于这些细菌中的Thio 16S基因或SoxB基因的至少一个片段。 该方法可用于确定土壤样品中元素硫的含量。
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4.发明申请公开(公告)号：US20110020913A1
公开(公告)日：2011-01-27
申请号：US12747990
申请日：2008-12-03
申请人： Giacomo Rispoli , Emiliano Fioravanti , Renzo Bignazzi , Ezio Nicola D'Addario , Francesca De Ferra , Federico Capuano
发明人： Giacomo Rispoli , Emiliano Fioravanti , Renzo Bignazzi , Ezio Nicola D'Addario , Francesca De Ferra , Federico Capuano
IPC分类号： C12N1/12
CPC分类号： C12N1/12 , C12M21/02 , C12M23/18 , C12M23/58 , C12M29/02 , C12M43/04 , C12M47/02 , C12M47/06
摘要： The invention relates to a process for the production of algal biomass with a high lipid content, comprising: (a) the production of inocula in order to effect phase (b), in photoreactors; (b) the massive cultivation of the algal biomass in open ponds, inoculated with phase (a); (c) a thickening phase of the algal biomass, effected blandly; (d) an induction phase of the lipid production, wherein modules are used consisting of photoreactors or open ponds; (e) a separation phase of the biomass with a high lipid content.
摘要翻译： 本发明涉及一种生产具有高脂质含量的藻类生物质的方法，包括：（a）在光反应器中生产接种物以实现阶段（b）; （b）接种阶段（a）的露天池塘中大量培养藻类生物量; （c）藻类生物质的增稠期，平缓地进行; （d）脂质生产的诱导期，其中使用由光反应器或露天池组成的模块; （e）具有高脂质含量的生物质的分离阶段。
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公开(公告)号：US5652116A
公开(公告)日：1997-07-29
申请号：US268542
申请日：1994-07-06
摘要： Disclosed is a novel method for the in vivo and in vitro synthesis of modified peptides, using either engineered microorganisms capable of expressing peptide synthetases with modified substrate specificity, or peptide synthetases extracted from said microorganisms.
摘要翻译： 公开了使用能够表达具有修饰的底物特异性的肽合成酶的工程微生物或从所述微生物提取的肽合成酶来体内和体外合成修饰肽的新方法。
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6.发明授权Cloning and sequencing of the gene which codes for a new pilinic subunit of bordella pertussis 失效编码百日咳杆菌新型番茄亚基的基因的克隆和测序
公开(公告)号：US5622706A
公开(公告)日：1997-04-22
申请号：US334425
申请日：1994-11-04
申请人： Paola Pedroni , Barbara Riboli , Francesca De Ferra , Guido Grandi , Salvatore Toma , Beatrice Arico , Rino Rappuoli
发明人： Paola Pedroni , Barbara Riboli , Francesca De Ferra , Guido Grandi , Salvatore Toma , Beatrice Arico , Rino Rappuoli
IPC分类号： C12N1/20 , A61K38/00 , A61K39/00 , A61P31/04 , A61P37/04 , C07K14/00 , C07K14/195 , C07K14/235 , C07K14/41 , C12N1/19 , C12N1/21 , C12P21/02 , C12R1/01 , C12R1/19 , A61K39/02 , A61K39/10 , C12N15/00
CPC分类号： C07K14/235 , A61K39/00
摘要： The cloning and sequencing of the gene which codes for a new pilinic subunit of Bordetella pertussis are described. The aminoacid sequence of the mature subunit, deduced from its necleotide sequence, is similar but not identical to that of the known pilins 2, 3 and 6. Polypeptides having the aminoacid sequence of the mature pilinic subunit or of regions thereof are particularly useful for the development of synthetic acellular vaccines against pertussis.
摘要翻译： 描述了编码百日咳博德特氏菌新型番茄亚基的基因的克隆和测序。 从其核苷酸序列推导的成熟亚单位的氨基酸序列与已知的pilins 2,3和6相似但不相同。具有成熟皮质亚单位或其区域的氨基酸序列的多肽特别可用于 开发针对百日咳的合成无细胞疫苗。
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7.发明授权Recombinant vector and use thereof for exocellular preparation of antibodies in single molecule form from Bacillus subtilis 失效重组载体及其用于从枯草芽孢杆菌单分子形式的细胞外抗体制备
公开(公告)号：US5674712A
公开(公告)日：1997-10-07
申请号：US207169
申请日：1994-03-08
IPC分类号： C07K7/06 , A61K38/00 , C07K7/08 , C07K14/00 , C07K14/195 , C07K14/41 , C07K16/00 , C07K16/46 , C12N1/21 , C12N15/02 , C12N15/09 , C12N15/13 , C12N15/75 , C12P21/02 , C12P21/08 , C12R1/125 , C12N15/63
CPC分类号： C07K16/468 , C12N15/75 , A61K38/00 , C07K2319/00 , C07K2319/036 , Y10S435/839 , Y10S530/808 , Y10S530/867
摘要： A recombinant vector for the expression and secretion of antibodies in single molecule form (scFv) from B. subtilis, where said vector comprises the promoter of the gene for neutral protease, a new secretion sequence (I) and a DNA sequence coding a scFv antibody of interest, a strain of B. subtilis transformed with said recombinant vector, and a process for the exocellular production of scFv antibodies by culturing said strain of B. subtilis are described. The recombinant vector allows the expression of scFv in a completely soluble form and its secretion in high yields.
摘要翻译： 一种用于从枯草芽孢杆菌分离形式（scFv）表达和分泌抗体的重组载体，其中所述载体包含用于中性蛋白酶的基因的启动子，新的分泌序列（I）和编码scFv抗体的DNA序列 描述了用所述重组载体转化的枯草芽孢杆菌菌株，以及通过培养所述枯草芽孢杆菌菌株来细胞外产生scFv抗体的方法。 重组载体允许以完全可溶形式表达scFv，并以高产率分泌其表达。
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公开(公告)号：US20100184060A1
公开(公告)日：2010-07-22
申请号：US12666067
申请日：2008-06-10
CPC分类号： C12Q1/689
摘要： The invention relates to a method for the identification of propane-oxidizing bacteria which is based on the identification of at least one fragment of the prmA gene encoding the alpha subunit of the propane monooxygenase enzyme and/or the prmD gene encoding an ancillary protein involved in the oxidation reaction of propane by gene amplification in the presence of pairs of primers selected in correspondence of homologous portions, deduced from the alignment of the prmA and prmD sequences.
摘要翻译： 本发明涉及一种鉴定丙烷氧化细菌的方法，该方法基于鉴定编码丙烷单加氧酶的α亚单位的prmA基因和/或编码参与其中的辅助蛋白质的prmD基因的至少一个片段 在对应于prmA和prmD序列推导的同源部分选择的引物对存在下，通过基因扩增进行丙烷的氧化反应。
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9.发明授权Engineered peptide synthetases and their use for the non-ribosomal production of peptides 失效工程肽合成酶及其用于非核糖体产生肽的用途
公开(公告)号：US5795738A
公开(公告)日：1998-08-18
申请号：US670901
申请日：1996-06-26
摘要： The present invention relates to the construction of functional engineered peptide synthetases capable of displaying a correct activity and their use for the non-ribosomal production of modified peptides.
摘要翻译： 本发明涉及能够显示正确活性的功能工程化肽合成酶的构建及其用于修饰肽非核糖体产生的用途。
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10.发明授权Promoter of the gene which codes for the pilinic subunit fim3 of Bordetella pertussis and its use for the expression in Bordetella of the genes which code for a protein of interest 失效编码百日咳博德特氏菌的pilinic亚基fim3的基因的启动子及其用于在博德特氏菌中表达编码感兴趣的蛋白质的基因的基因
公开(公告)号：US5395764A
公开(公告)日：1995-03-07
申请号：US213811
申请日：1994-03-16
CPC分类号： C12N15/74 , C07K14/235 , A61K39/00
摘要： The isolation and characterization of the promoter regions of the genes which code for the pilinic subunits fim2, fim3 and fimx of Bordetella pertussis, are described, as well as the construction of vectors containing the regions, and microorganisms transformed by the vectors.The promoter regions, or nucleotide fragments thereof, are particularly useful for the regulable or non-regulable expression of genes which code for a protein of interest in a strain of Bordetella. The transformed Bordetella strains are particularly suitable for the development of an effective anti-pertussis vaccine.
摘要翻译： 描述了编码百日咳博德特氏菌的pilinic亚基fim2，fim3和fimx的基因的启动子区域的分离和表征，以及构建含有这些区域的载体和由载体转化的微生物。 启动子区域或其核苷酸片段对编码在博德特氏菌菌株中感兴趣的蛋白质的基因的可调节或不可调节的表达特别有用。 转化的博德氏菌属菌株特别适用于开发有效的抗百日咳疫苗。
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