利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

13 条记录 (耗时 0.016 秒)

    METHOD FOR TRANSFER OF MOLECULAR SUBSTANCES WITH PROKARYONTIC NUCLEIC ACID-BINDING PROTEINS
    1.
    发明申请
    METHOD FOR TRANSFER OF MOLECULAR SUBSTANCES WITH PROKARYONTIC NUCLEIC ACID-BINDING PROTEINS 失效
    用衍生核酸结合蛋白转移分子物质的方法

    公开(公告)号：US20090155912A1

    公开(公告)日：2009-06-18

    申请号：US12207467

    申请日：2008-09-09

    申请人： Gerald Boehm ,  Dirk Esser

    发明人： Gerald Boehm ,  Dirk Esser

    IPC分类号： C12N15/74

    CPC分类号： C07K19/00 ,  C07K14/005 ,  C12N15/87 ,  C12N2710/10022 ,  C12N2710/16222 ,  C12N2710/16622 ,  C12N2740/16022

    摘要： The invention relates to a method for the transfer of molecular substances, for example proteins or nucleic acids in cells, in the case of using DNA combined with a possible gene expression. A prokaryotic nucleic acid-binding protein is used for the transfer, which is preferably obtained from a thermostable organism. Where the substance to be transferred is a nucleic acid, the protein forms a reversible complex with the nucleic acid. The prokaryotic protein condenses and compacts the nucleic acids. Said nucleic acids can be taken up in the target cells after suitable incubation.

    摘要翻译： 本发明涉及在使用结合可能的基因表达的DNA的情况下转移分子物质例如细胞中的蛋白质或核酸的方法。 原核的核酸结合蛋白用于转移，其优选从热稳定生物获得。 当要转移的物质是核酸时，蛋白质与核酸形成可逆复合物。 原核蛋白凝结并压制核酸。 所述核酸可以在合适的温育后在靶细胞中吸收。

    Method for transfer of molecular substances with prokaryotic nucleic acid-binding proteins
    2.
    发明授权
    Method for transfer of molecular substances with prokaryotic nucleic acid-binding proteins 失效
    用原核核酸结合蛋白转移分子物质的方法

    公开(公告)号：US07981676B2

    公开(公告)日：2011-07-19

    申请号：US12207467

    申请日：2008-09-09

    申请人： Gerald Boehm ,  Dirk Esser

    发明人： Gerald Boehm ,  Dirk Esser

    IPC分类号： C12N15/00

    CPC分类号： C07K19/00 ,  C07K14/005 ,  C12N15/87 ,  C12N2710/10022 ,  C12N2710/16222 ,  C12N2710/16622 ,  C12N2740/16022

    摘要： The invention relates to a method for the transfer of molecular substances, for example proteins or nucleic acids in cells, in the case of using DNA combined with a possible gene expression. A prokaryotic nucleic acid-binding protein is used for the transfer, which is preferably obtained from a thermostable organism. Where the substance to be transferred is a nucleic acid, the protein forms a reversible complex with the nucleic acid. The prokaryotic protein condenses and compacts the nucleic acids. Said nucleic acids can be taken up in the target cells after suitable incubation.

    摘要翻译： 本发明涉及在使用结合可能的基因表达的DNA的情况下转移分子物质例如细胞中的蛋白质或核酸的方法。 原核的核酸结合蛋白用于转移，其优选从热稳定生物获得。 当要转移的物质是核酸时，蛋白质与核酸形成可逆复合物。 原核蛋白凝结并压制核酸。 所述核酸可以在合适的温育后在靶细胞中吸收。

    Method for characterizing and separating molecular associates
    6.
    发明授权
    Method for characterizing and separating molecular associates 失效
    表征和分离分子缔合物的方法

    公开(公告)号：US07488605B2

    公开(公告)日：2009-02-10

    申请号：US10950347

    申请日：2004-09-23

    申请人： Gerald Boehm ,  Ulrich Schmidt

    发明人： Gerald Boehm ,  Ulrich Schmidt

    IPC分类号： G01N33/566

    CPC分类号： G01N33/56983 ,  G01N33/537 ,  G01N33/6803 ,  G01N33/6896 ,  G01N2333/4709

    摘要： The present invention relates to a method for characterising and optionally separating molecular associates, especially particles having a size smaller than 300 nm. Partial units of the molecular associates are used as markers, whereby said units are marked with fluorescent dyes. The marked associates and aggregates are characterised by means of a FACS (fluorescence-activated cell sorter).

    摘要翻译： 本发明涉及用于表征和任选分离分子缔合体的方法，特别是尺寸小于300nm的颗粒。 分子缔合物的部分单元用作标记，其中所述单元用荧光染料标记。 标记的缔合物和聚集体通过FACS（荧光激活细胞分选仪）的特征。

    Method for transfer of molecular substances with prokaryotic nucleic acid-binding proteins
    7.
    发明授权
    Method for transfer of molecular substances with prokaryotic nucleic acid-binding proteins 失效
    用原核核酸结合蛋白转移分子物质的方法

    公开(公告)号：US07435595B2

    公开(公告)日：2008-10-14

    申请号：US10954549

    申请日：2004-09-29

    申请人： Gerald Boehm ,  Dirk Esser

    发明人： Gerald Boehm ,  Dirk Esser

    IPC分类号： C12N15/00 ,  C12N15/88

    CPC分类号： C07K19/00 ,  C07K14/005 ,  C12N15/87 ,  C12N2710/10022 ,  C12N2710/16222 ,  C12N2710/16622 ,  C12N2740/16022

    摘要： The invention relates to a method for the transfer of molecular substances, for example proteins or nucleic acids in cells, in the case of using DNA combined with a possible gene expression. A prokaryotic nucleic acid-binding protein is used for the transfer, which is preferably obtained from a thermostable organism. Where the substance to be transferred is a nucleic acid, the protein forms a reversible complex with the nucleic acid. The prokaryotic protein condenses and compacts the nucleic acids. Said nucleic acids can be taken up in the target cells after suitable incubation.

    摘要翻译： 本发明涉及在使用结合可能的基因表达的DNA的情况下转移分子物质例如细胞中的蛋白质或核酸的方法。 原核的核酸结合蛋白用于转移，其优选从热稳定生物获得。 当要转移的物质是核酸时，蛋白质与核酸形成可逆复合物。 原核蛋白凝结并压制核酸。 所述核酸可以在合适的温育后在靶细胞中吸收。

    Methods for establishing and analyzing the conformation of amino acid sequences
    8.
    发明申请
    Methods for establishing and analyzing the conformation of amino acid sequences 审中-公开
    建立和分析氨基酸序列构象的方法

    公开(公告)号：US20060253260A1

    公开(公告)日：2006-11-09

    申请号：US11370582

    申请日：2006-03-08

    申请人： Gerald Boehm ,  Roman Dalluege

    发明人： Gerald Boehm ,  Roman Dalluege

    IPC分类号： G06F19/00

    CPC分类号： G16B15/00 ,  G16B50/00

    摘要： The present invention relates to methods for establishing and analyzing the conformation of amino acid sequences. In particular, the invention relates to methods for the validation of the conformation of given amino acid-based molecules, methods for conformation determination starting from a linear amino acid sequence as well as methods for the alignment of two or more amino acid sequences.

    摘要翻译： 本发明涉及建立和分析氨基酸序列构象的方法。 特别地，本发明涉及用于验证给定的基于氨基酸的分子的构象的方法，从线性氨基酸序列开始的构象确定方法以及两个或更多个氨基酸序列比对的方法。

    METHOD FOR THE DETECTION OF AMYLOID-B OLIGOMERS IN BODY FLUIDS
    10.
    发明申请
    METHOD FOR THE DETECTION OF AMYLOID-B OLIGOMERS IN BODY FLUIDS 审中-公开
    用于检测体液中AMYLOID-B低聚物的方法

    公开(公告)号：US20100129847A1

    公开(公告)日：2010-05-27

    申请号：US12375391

    申请日：2007-07-27

    申请人： Alexander Navarrete Santos ,  Gerald Boehm

    发明人： Alexander Navarrete Santos ,  Gerald Boehm

    IPC分类号： C12Q1/02 ,  G01N33/53

    CPC分类号： G01N33/6896 ,  G01N33/542 ,  G01N2800/2821

    摘要： The present invention relates to a method for the detection of marker of the Alzheimer's disease, namely the amyloid-β oligomers in human CSF, using a combination of steps including demasking the epitopes responsible for antibody binding on the Aβ peptide oligomers as well as detecting fluorescently marked antibodies binding to said epitopes, preferably by using the FRET technology.

    摘要翻译： 本发明涉及一种检测阿尔茨海默氏病标志物的方法，即淀粉样蛋白 寡聚体在人类CSF中，使用步骤的组合，包括解除负责抗体结合在Aβ上的表位; 肽寡聚物以及检测结合所述表位的荧光标记抗体，优选通过使用FRET技术。