Identification of objects of interest using multiple illumination schemes and finding overlap of features in corresponding multiple images
    3.
    发明授权
    Identification of objects of interest using multiple illumination schemes and finding overlap of features in corresponding multiple images 有权
    使用多个照明方案识别感兴趣的对象,并在相应的多个图像中发现特征的重叠

    公开(公告)号:US06259807B1

    公开(公告)日:2001-07-10

    申请号:US09443949

    申请日:1999-11-19

    申请人: Ilya Ravkin

    发明人: Ilya Ravkin

    IPC分类号: G06K900

    摘要: Detection of fetal nucleated red blood cells (NRBCs) is achieved by employing a combination of brightfield and fluorescence images of nuclear and cytoplasmic markers. The brightfield and fluorescence images are all obtained with a single multibandpass dichroic mirror. The objects in the sample are stained with a fluorescent dye that selectively stains nuclei and a dye that selectively stains fetal hemoglobin in the cytoplasm of fetal RBCs. UV excitation provides fluorescent emissions from the stained cell nuclei and visible illumination provides brightfield transmission of light that is absorbed by the stained cytoplasm. The images are processed to determine regions where the fluorescent emissions by cell nuclei in response to the UV excitation and the absorption by fetal hemoglobin of the brightfield illumination overlap or are in close proximity. The brightfield and fluorescence images may be sequentially acquired or derived from a single image where the UV excitation and visible illumination occur simultaneously.

    摘要翻译: 通过使用核和细胞质标记物的明场和荧光图像的组合来实现胎儿有核红细胞(NRBC)的检测。 明场和荧光图像均用单个多波束分色镜获得。 样本中的物体用选择性染色核的荧光染料和在胎儿RBC细胞质中选择性地染色胎儿血红蛋白的染料染色。 UV激发提供来自染色的细胞核的荧光发射,并且可见光照提供被染色的细胞质吸收的光的明场透射。 处理图像以确定响应于UV激发的细胞核的荧光发射和亮场照射的胎儿血红蛋白的吸收重叠或紧密接近的区域。 亮场和荧光图像可以从UV激发和可见光同时发生的单一图像顺序地获得或导出。

    Identification of objects of interest using multiple illumination schemes and finding overlap of features in corresponding multiple images
    8.
    发明授权
    Identification of objects of interest using multiple illumination schemes and finding overlap of features in corresponding multiple images 有权
    使用多个照明方案识别感兴趣的对象,并在相应的多个图像中发现特征的重叠

    公开(公告)号:US06633662B2

    公开(公告)日:2003-10-14

    申请号:US09901584

    申请日:2001-07-09

    申请人: Ilya Ravkin

    发明人: Ilya Ravkin

    IPC分类号: G06K900

    摘要: Detection of fetal nucleated red blood cells (NRBCs) is achieved by employing a combination of brightfield and fluorescence images of nuclear and cytoplasmic markers. The brightfield and fluorescence images are all obtained with a single multi-bandpass dichroic mirror. The objects in the sample are stained with a fluorescent dye that selectively stains nuclei and a dye that selectively stains fetal hemoglobin in the cytoplasm of fetal RBCs. UV excitation provides fluorescent emissions from the stained cell nuclei and visible illumination provides brightfield transmission of light that is absorbed by the stained cytoplasm. The images are processed to determine regions where the fluorescent emissions by cell nuclei in response to the UV excitation and the absorption by fetal hemoglobin of the brightfield illumination overlap or are in close proximity. The brightfield and fluorescence images may be sequentially acquired or derived from a single image where the UV excitation and visible illumination occur simultaneously.

    摘要翻译: 通过使用核和细胞质标记物的明场和荧光图像的组合来实现胎儿有核红细胞(NRBC)的检测。 明场和荧光图像均使用单个多带通二向色镜获得。 样本中的物体用选择性染色核的荧光染料和在胎儿RBC细胞质中选择性染色胎儿血红蛋白的染料染色。 UV激发提供来自染色的细胞核的荧光发射,并且可见光照提供被染色的细胞质吸收的光的明场透射。 处理图像以确定响应于UV激发的细胞核的荧光发射和亮场照射的胎儿血红蛋白的吸收重叠或紧密接近的区域。 亮场和荧光图像可以顺序地获得或从单一图像导出,其中UV激发和可见光同时发生。

    Identification of objects of interest using multiple illumination schemes and finding overlap of features in corresponding multiple images
    9.
    发明授权
    Identification of objects of interest using multiple illumination schemes and finding overlap of features in corresponding multiple images 失效
    使用多个照明方案识别感兴趣的对象,并在相应的多个图像中发现特征的重叠

    公开(公告)号:US06169816A

    公开(公告)日:2001-01-02

    申请号:US09072502

    申请日:1998-05-04

    申请人: Ilya Ravkin

    发明人: Ilya Ravkin

    IPC分类号: G06K900

    摘要: Detection of fetal nucleated red blood cells (NRBCs) is achieved by employing a combination of brightfield and fluorescence images of nuclear and cytoplasmic markers. The brightfield and fluorescence images are all obtained with a single multi-bandpass dichroic mirror. The objects in the sample are stained with a fluorescent dye that selectively stains nuclei and a dye that selectively stains fetal hemoglobin in the cytoplasm of fetal RBCs. UV excitation provides fluorescent emissions from the stained cell nuclei and visible illumination provides brightfield transmission of light that is absorbed by the stained cytoplasm. The images are processed to determine regions where the fluorescent emissions by cell nuclei in response to the UV excitation and the absorption by fetal hemoglobin of the brightfield illumination overlap or are in close proximity. The brightfield and fluorescence images may be sequentially acquired or derived from a single image where the UV excitation and visible illumination occur simultaneously.

    摘要翻译: 通过使用核和细胞质标记物的明场和荧光图像的组合来实现胎儿有核红细胞(NRBC)的检测。 明场和荧光图像均使用单个多带通二向色镜获得。 样本中的物体用选择性染色核的荧光染料和在胎儿RBC细胞质中选择性地染色胎儿血红蛋白的染料染色。 UV激发提供来自染色的细胞核的荧光发射,并且可见光照提供被染色的细胞质吸收的光的明场透射。 处理图像以确定响应于UV激发的细胞核的荧光发射和亮场照射的胎儿血红蛋白的吸收重叠或紧密接近的区域。 亮场和荧光图像可以从UV激发和可见光同时发生的单一图像顺序地获得或导出。

    Automatic focusing system using focal length-changing element
    10.
    发明授权
    Automatic focusing system using focal length-changing element 失效
    使用焦距变化元件的自动聚焦系统

    公开(公告)号:US5254845A

    公开(公告)日:1993-10-19

    申请号:US931944

    申请日:1992-08-18

    IPC分类号: G02B7/38 G01J1/20

    CPC分类号: G02B7/38

    摘要: In an automatic focusing system an element (2) is inserted in the path of a beam of light (3) directed towards a sensor array. The element comprises a transparent material or predetermined reflective index, the element presenting a first region (4) of a first thickness and another region (5) of a greater thickness. The image falling on to the sensor comprises three areas--one area where the light has passed through the one region and another area where the light has passed through the further region and yet another area. The automatic focusing system derives signals from two of the areas and processes the signals so that the image is in focus in the third area.

    摘要翻译: 在自动对焦系统中,元件(2)插入到指向传感器阵列的光束(3)的路径中。 该元件包括透明材料或预定的反射指数,该元件具有第一厚度的第一区域(4)和更大厚度的另一区域(5)。 落在传感器上的图像包括三个区域 - 一个区域,其中光已经通过一个区域,另一个区域,其中光已经通过另一区域,另一个区域。 自动对焦系统从两个区域中得到信号,并对信号进行处理,使得图像在第三区域中聚焦。