公开(公告)号：US06938987B2

公开(公告)日：2005-09-06

申请号：US10623487

申请日：2003-07-18

Applicant: Richard N. Ellson ,  James K. Foote ,  Mitchell W. Mutz

Inventor： Richard N. Ellson ,  James K. Foote ,  Mitchell W. Mutz

IPC: B01J19/00 ,  B05B17/06 ,  B41J2/04 ,  B41J2/14 ,  C07B61/00 ,  C40B40/06 ,  C40B40/10 ,  C40B60/14 ,  B41J2/135

CPC classification number: C40B80/00 ,  B01J19/0046 ,  B01J2219/00351 ,  B01J2219/00527 ,  B01J2219/0059 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00608 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00617 ,  B01J2219/00621 ,  B01J2219/00626 ,  B01J2219/0063 ,  B01J2219/00637 ,  B01J2219/00641 ,  B01J2219/00659 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01L2400/0433 ,  B05B17/0607 ,  B05B17/0615 ,  B41J2/04 ,  B41J2/14008 ,  C07B2200/11 ,  C40B40/06 ,  C40B40/10 ,  C40B60/14

Abstract: The present invention provides a method and device for the acoustic ejection of fluid droplets from each of a plurality of fluid-containing reservoirs. The droplets are ejected toward sites on a substrate surface for deposition thereon. The device is comprised of: a plurality of reservoirs each adapted to contain a fluid; an ejector comprising a means for generating acoustic radiation and a means for focusing the generated acoustic radiation so as to eject fluid droplets from the reservoir fluids; and a means for positioning the reservoirs in acoustically coupled relationship to the ejector. The invention is useful in a number of contexts, particularly in the preparation of biomolecular arrays.

Abstract translation: 本发明提供了一种用于从多个含液体储存器中的每一个的声弹出流体液滴的方法和装置。 液滴被喷射到衬底表面上的位置以便沉积在衬底表面上。 该装置包括：多个储存器，每个储存器适于容纳流体; 喷射器，其包括用于产生声辐射的装置和用于聚焦所产生的声辐射以便从储层流体喷射流体液滴的装置; 以及用于将储存器定位成与喷射器的声耦合关系的装置。 本发明在许多上下文中是有用的，特别是在生物分子阵列的制备中。

公开(公告)号：US06746104B2

公开(公告)日：2004-06-08

申请号：US09964215

申请日：2001-09-25

Applicant: Richard N. Ellson ,  Mitchell W. Mutz ,  James K. Foote

Inventor： Richard N. Ellson ,  Mitchell W. Mutz ,  James K. Foote

IPC: B41J2135

CPC classification number: B01J19/0046 ,  B01J2219/00351 ,  B01J2219/00527 ,  B01J2219/0059 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00608 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00617 ,  B01J2219/00621 ,  B01J2219/00626 ,  B01J2219/0063 ,  B01J2219/00637 ,  B01J2219/00641 ,  B01J2219/00659 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01L2400/0433 ,  B05B17/0607 ,  B05B17/0615 ,  B41J2/04 ,  B41J2/14008 ,  C07B2200/11 ,  C40B40/06 ,  C40B40/10 ,  C40B60/14 ,  C40B80/00 ,  Y10T436/2575

Abstract: The present invention provides a unique and highly accurate method for generating molecular arrays of very high density on porous surfaces. The method involves the application of focused acoustic energy to each of a plurality of fluid-containing reservoirs to eject a small fluid droplet—on the order of 1 picoliter or less—from each reservoir to a site on a porous substrate surface. High density molecular arrays are provided as well, in which greater than about 62,500 molecular moieties, serving as array elements, are present on a porous surface. Biomolecular arrays that can be generated using focused acoustic ejection include oligonucleotide arrays and peptidic arrays.

Abstract translation: 本发明提供了一种用于在多孔表面上产生非常高密度的分子阵列的独特和高精度的方法。 该方法涉及将聚焦的声能施加到多个含液体储存器中的每一个，以将从每个储存器至1皮升或更小量级的小液滴喷射到多孔基材表面上的位置。 还提供了高密度分子阵列，其中作为阵列元素的大于约62,500个分子部分存在于多孔表面上。 可以使用聚焦声弹射产生的生物分子阵列包括寡核苷酸阵列和肽阵列。

公开(公告)号：US06666541B2

公开(公告)日：2003-12-23

申请号：US09964212

申请日：2001-09-25

Applicant: Richard N. Ellson ,  James K. Foote ,  Mitchell W. Mutz

Inventor： Richard N. Ellson ,  James K. Foote ,  Mitchell W. Mutz

IPC: B41J2135

CPC classification number: C40B80/00 ,  B01J19/0046 ,  B01J2219/00351 ,  B01J2219/00527 ,  B01J2219/0059 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00608 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00617 ,  B01J2219/00621 ,  B01J2219/00626 ,  B01J2219/0063 ,  B01J2219/00637 ,  B01J2219/00641 ,  B01J2219/00659 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01L2400/0433 ,  B05B17/0607 ,  B05B17/0615 ,  B41J2/04 ,  B41J2/14008 ,  C07B2200/11 ,  C40B40/06 ,  C40B40/10 ,  C40B60/14

Abstract: The present invention provides a method and device for the acoustic ejection of fluid droplets from each of a plurality of fluid-containing reservoirs. The droplets are ejected toward sites on a substrate surface for deposition thereon. The device is comprised of: a plurality of reservoirs each adapted to contain a fluid; an ejector comprising a means for generating acoustic radiation and a means for focusing the generated acoustic radiation so as to eject fluid droplets from the reservoir fluids; and a means for positioning the ejector in acoustically coupled relationship to each of the reservoirs. The invention is useful in a number of contexts, particularly in the preparation of biomolecular arrays.

公开(公告)号：US06548308B2

公开(公告)日：2003-04-15

申请号：US09962730

申请日：2001-09-24

Applicant: Richard N. Ellson ,  Mitchell W. Mutz ,  James K. Foote

Inventor： Richard N. Ellson ,  Mitchell W. Mutz ,  James K. Foote

IPC: B01L302

CPC classification number: B41J2/14008 ,  B01J13/04 ,  B01J19/0046 ,  B01J2219/00351 ,  B01J2219/00527 ,  B01J2219/00529 ,  B01J2219/0059 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00608 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00626 ,  B01J2219/00637 ,  B01J2219/00641 ,  B01J2219/00659 ,  B01J2219/00722 ,  B01J2219/00725 ,  B05B17/0615 ,  B22F9/08 ,  B22F2009/0804 ,  B22F2999/00 ,  C40B40/06 ,  C40B40/10 ,  C40B60/14 ,  Y10T436/143333 ,  Y10T436/2575 ,  B22F2202/01

Abstract: A method and device for generating droplets of immiscible fluids are provided. Extremely fine droplets may be generated, on the order of 1 picoliter or less, using focused acoustic energy to eject the droplets from a reservoir containing two or more immiscible fluids. Optionally, the droplets may be ejected onto discrete sites on a substrate surface so as to form an array thereon.

公开(公告)号：US07900505B2

公开(公告)日：2011-03-08

申请号：US11329817

申请日：2006-01-10

Applicant: Mitchell W. Mutz ,  Richard N. Ellson ,  James K. Foote

Inventor： Mitchell W. Mutz ,  Richard N. Ellson ,  James K. Foote

IPC: G01N29/00

CPC classification number: G01N29/028 ,  G01N11/10 ,  G01N29/032 ,  G01N29/222 ,  G01N2291/02416 ,  G01N2291/048 ,  G01N2291/102

Abstract: The invention provides devices and methods for acoustically assessing the contents in a plurality of reservoirs. Each reservoir has a portion adapted to contain a fluid, and an acoustic radiation generator is positioned in acoustic coupling relationship to each of the reservoirs. Acoustic radiation generated by the acoustic radiation generator is transmitted through at least the portion of each reservoir to an analyzer. The analyzer is capable of analyzing a characteristic of the transmitted acoustic radiation and optionally correlating the characteristic to a property of the reservoirs' contents. The invention is particularly suited for assessing the contents of a plurality of reservoirs to allow for accuracy and control over the dispensing of fluids therefrom.

Abstract translation: 本发明提供用于声学评估多个储存器中的内容物的装置和方法。 每个储存器具有适于容纳流体的部分，并且声辐射发生器被定位成与每个储存器的声耦合关系。 由声辐射发生器产生的声辐射通过至少每个储存器的部分传输到分析器。 分析仪能够分析所发射的声辐射的特性，并且可选地将特征与储层内容物性相关联。 本发明特别适用于评估多个储存器的内容物，以便准确地控制流体的分配。

公开(公告)号：US06938995B2

公开(公告)日：2005-09-06

申请号：US10310638

申请日：2002-12-04

Applicant: Mitchell W. Mutz ,  Richard N. Ellson ,  James K. Foote

Inventor： Mitchell W. Mutz ,  Richard N. Ellson ,  James K. Foote

IPC: B01L3/02 ,  C40B60/14 ,  G01F23/296 ,  G01N29/02 ,  G01N29/22 ,  G01N29/28 ,  G01N35/00 ,  G01N35/10 ,  B41J2/02

CPC classification number: B01L3/0268 ,  B01J2219/00362 ,  B01J2219/00702 ,  B01L2200/143 ,  B01L2300/0819 ,  B01L2400/0436 ,  B01L2400/0439 ,  C40B60/14 ,  G01F23/296 ,  G01F23/2968 ,  G01N29/02 ,  G01N29/22 ,  G01N29/222 ,  G01N29/28 ,  G01N35/1016 ,  G01N2035/00158 ,  G01N2291/02818 ,  G01N2291/02836 ,  G01N2291/02881 ,  G01N2291/2695

Abstract: The invention provides devices and methods for acoustically assessing the contents of a plurality of reservoirs that are typically provided as an array. An acoustic radiation generator is employed for generating acoustic radiation having an image field of a size sufficient to interrogate a plurality of selected reservoirs at one time. The generator is placed in acoustic coupling relationship via a fluid acoustic coupling medium to the selected reservoirs, and acoustic radiation generated by the generator is transmitted through the coupling medium, an exterior surface of the selected reservoirs, and the selected reservoirs. A characteristic of the transmitted acoustic radiation is analyzed so as to assess the contents of each of the selected reservoirs. Fluid may be ejected from the selected reservoirs according to the assessment. Optionally, a means is provided for removing fluid acoustic coupling medium from the exterior surface after acoustic assessment.

Abstract translation: 本发明提供用于声学评估通常作为阵列提供的多个储存器的内容物的装置和方法。 声辐射发生器用于产生具有足以在一个时间询问多个所选择的储层的尺寸的图像场的声辐射。 发电机通过流体声耦合介质放置到声学耦合关系到所选择的储存器，并且由发生器产生的声辐射通过耦合介质，所选择的储存器的外表面和所选择的储存器传输。 分析所发射的声辐射的特征，以便评估每个所选择的储层的内容物。 流体可以根据评估从所选择的储层中排出。 可选地，提供了用于在声学评估之后从外表面去除流体声学耦合介质的装置。

公开(公告)号：US06802593B2

公开(公告)日：2004-10-12

申请号：US10269413

申请日：2002-10-11

Applicant: Richard N. Ellson ,  James K. Foote ,  Mitchell W. Mutz

Inventor： Richard N. Ellson ,  James K. Foote ,  Mitchell W. Mutz

IPC: B41J2135

CPC classification number: C40B80/00 ,  B01J19/0046 ,  B01J2219/00351 ,  B01J2219/00527 ,  B01J2219/0059 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00608 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00617 ,  B01J2219/00621 ,  B01J2219/00626 ,  B01J2219/0063 ,  B01J2219/00637 ,  B01J2219/00641 ,  B01J2219/00659 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01L2400/0433 ,  B05B17/0607 ,  B05B17/0615 ,  B41J2/04 ,  B41J2/14008 ,  C07B2200/11 ,  C40B40/06 ,  C40B40/10 ,  C40B60/14

Abstract: The present invention provides a method and device for the acoustic ejection of fluid droplets from each of a plurality of fluid-containing reservoirs. The droplets are ejected toward sites on a substrate surface for deposition thereon. The device is comprised of: a plurality of reservoirs each adapted to contain a fluid; an ejector comprising a means for generating acoustic radiation and a means for focusing the generated acoustic radiation so as to eject fluid droplets from the reservoir fluids; and a means for positioning the ejector in acoustically coupled relationship to each of the reservoirs. The invention is useful in a number of contexts, particularly in the preparation of biomolecular arrays.

Abstract translation: 本发明提供了一种用于从多个含液体储存器中的每一个的声弹出流体液滴的方法和装置。 液滴被喷射到衬底表面上的位置以便沉积在衬底表面上。 该装置包括：多个储存器，每个储存器适于容纳流体; 喷射器，其包括用于产生声辐射的装置和用于聚焦所产生的声辐射以便从储层流体喷射流体液滴的装置; 以及用于将喷射器定位成与每个储存器的声耦合关系的装置。 本发明在许多上下文中是有用的，特别是在生物分子阵列的制备中。

公开(公告)号：US06642061B2

公开(公告)日：2003-11-04

申请号：US10112693

申请日：2002-03-28

Applicant: Richard N. Ellson ,  Mitchell W. Mutz ,  James K. Foote

Inventor： Richard N. Ellson ,  Mitchell W. Mutz ,  James K. Foote

IPC: B01L302

CPC classification number: B41J2/14008 ,  B01J13/04 ,  B01J19/0046 ,  B01J2219/00351 ,  B01J2219/00527 ,  B01J2219/00529 ,  B01J2219/0059 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00608 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00626 ,  B01J2219/00637 ,  B01J2219/00641 ,  B01J2219/00659 ,  B01J2219/00722 ,  B01J2219/00725 ,  B05B17/0615 ,  B22F9/08 ,  B22F2009/0804 ,  B22F2999/00 ,  C40B40/06 ,  C40B40/10 ,  C40B60/14 ,  Y10T436/143333 ,  Y10T436/2575 ,  B22F2202/01

Abstract: The invention provides a method for generating droplets. Extremely fine droplets may be generated (on the order of 1 picoliter or less) using focused acoustic energy to eject the droplets from a reservoir containing two or more immiscible fluids. The droplets may include immiscible fluids or a single fluid. Typically, the droplets are ejected onto discrete sites on a substrate surface so as to form an array thereon. In some instances, the reservoirs contain layers of immiscible fluids, wherein an upper layer exhibits a nonuniform thickness. In such a case, fluid from a lower fluid layer may be propelled through an aperture region of the upper layer.