公开(公告)号：US11890870B2

公开(公告)日：2024-02-06

申请号：US17289552

申请日：2019-10-29

申请人： Labcyte Inc.

发明人： Sammy S. Datwani ,  Carson Riche ,  Marsha N. Blauwkamp ,  Justin Blonigan

IPC分类号： B41J2/045 ,  B01L3/02 ,  B01J19/00 ,  B01J19/26

CPC分类号： B41J2/04575 ,  B01J19/0046 ,  B01J19/26 ,  B01L3/0268 ,  B41J2/04516 ,  B41J2/04588 ,  B01J2219/00362 ,  B01L2200/0647 ,  B01L2200/148 ,  B01L2400/0436 ,  B01L2400/0439

摘要： Methods of ejecting droplets containing a non-Newtonian fluid by an acoustic droplet ejector can include applying a tone burst of focused acoustic energy to a fluid reservoir containing a non-Newtonian fluid at sufficient amplitude to effect droplet ejection according to a tone burst pattern. The tone burst pattern may include three discrete tone burst segments, the first tone burst segment having greater duration than the second and third segments, and third segment having greater duration than the second segment. The exact durations and amplitudes of the tone burst segments can be tuned to influence the ejection properties.

公开(公告)号：US09920352B2

公开(公告)日：2018-03-20

申请号：US13858832

申请日：2013-04-08

申请人： Labcyte Inc.

发明人： Mitchell W. Mutz ,  Richard N. Ellson

IPC分类号： C12Q1/22 ,  B01L3/02 ,  B01L3/00 ,  C40B40/06 ,  C40B40/10 ,  C40B60/14

CPC分类号： C12Q1/22 ,  B01J2219/00362 ,  B01J2219/00378 ,  B01J2219/00659 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01L3/0268 ,  B01L3/0293 ,  B01L3/508 ,  B01L2200/0647 ,  B01L2400/0436 ,  C40B40/06 ,  C40B40/10 ,  C40B60/14

摘要： Method and system for monitoring for a change in the amount and/or concentration of a pathogen in a pathogenic fluid. The method includes providing a pathogen-impermeable enclosure enclosing the pathogenic fluid, wherein the pathogenic fluid includes a pathogen and a carrier fluid. Additionally, the method includes acoustically monitoring for a change in the amount and/or concentration of the pathogen enclosed in the pathogen-impermeable enclosure. The acoustically monitoring for a change in the amount and/or concentration of the pathogen enclosed in the pathogen-impermeable enclosure includes generating acoustic radiation directed towards the pathogen-impermeable enclosure, transmitting the acoustic radiation through the pathogen-impermeable enclosure or reflecting the acoustic radiation by the pathogenic fluid, receiving the acoustic radiation transmitted through the pathogen-impermeable enclosure or receiving the acoustic radiation reflected by the pathogenic fluid, and analyzing the received acoustic radiation.

公开(公告)号：US09861987B2

公开(公告)日：2018-01-09

申请号：US14595063

申请日：2015-01-12

申请人： Labcyte Inc.

发明人： Richard N. Ellson

IPC分类号： B01L3/02 ,  B01L3/00

CPC分类号： B01L3/50857 ,  B01J2219/00313 ,  B01J2219/00319 ,  B01J2219/00362 ,  B01L3/0244 ,  B01L3/0268 ,  B01L3/50855 ,  B01L2400/0436

摘要： Provided herein is generally tubular container, preferably including a plurality of reservoirs defined therein. The container can be adapted for acoustic ejection of a fluid disposed within at least one of the reservoirs of the plurality of reservoirs. Alternatively, the container can be adapted for extraction of a fluid disposed within at least one of the reservoirs of the plurality of reservoirs using a non-acoustic liquid handling method.

公开(公告)号：US20110166797A1

公开(公告)日：2011-07-07

申请号：US12834786

申请日：2010-07-12

申请人： Richard N. Ellson ,  Richard G. Stearns

发明人： Richard N. Ellson ,  Richard G. Stearns

IPC分类号： G01N29/02 ,  G06F19/00 ,  G01K11/22

CPC分类号： B01L3/0268 ,  B01J2219/00362 ,  B01J2219/00702 ,  B01L2200/143 ,  B01L2300/0819 ,  B01L2400/0436 ,  B01L2400/0439 ,  C40B60/14 ,  G01F23/296 ,  G01F23/2968 ,  G01N29/02 ,  G01N29/22 ,  G01N29/222 ,  G01N29/28 ,  G01N35/1016 ,  G01N2035/00158 ,  G01N2291/02818 ,  G01N2291/02836 ,  G01N2291/02881 ,  G01N2291/2695

摘要： The invention provides devices and methods for acoustically determining the properties of the contents of one or more reservoirs in a plurality of reservoirs. Each reservoir is adapted to contain a fluid. An acoustic radiation generator can be positioned in acoustic coupling relationship to each of the reservoirs. Acoustic radiation generated by the acoustic radiation generator is transmitted through each reservoir to an analyzer. The analyzer is capable of analyzing a characteristic of the transmitted acoustic radiation and optionally correlating the characteristic to a property of the reservoirs' contents. Properties that may be determined include volume, temperature, and composition. The invention is particularly suited to determining the properties of the contents of a plurality of reservoirs to allow for accuracy and control over the dispensing of fluids therefrom.

摘要翻译： 本发明提供用于声学确定多个储存器中的一个或多个储存器的内容物的性质的装置和方法。 每个储存器适于容纳流体。 声辐射发生器可以定位成与每个储存器的声耦合关系。 由声辐射发生器产生的声辐射通过每个储存器传送到分析器。 分析仪能够分析所发射的声辐射的特性，并且可选地将特征与储层内容物性相关联。 可以确定的属性包括体积，温度和组成。 本发明特别适合于确定多个储存器的内容物的性质，以允许精确度和控制从其中分配流体。

公开(公告)号：US07717544B2

公开(公告)日：2010-05-18

申请号：US10956616

申请日：2004-10-01

申请人： Richard G. Stearns ,  Shehrzad Ahmed Qureshi

发明人： Richard G. Stearns ,  Shehrzad Ahmed Qureshi

IPC分类号： B41J2/045 ,  B41J2/135

CPC分类号： B05B12/004 ,  B01J2219/00362 ,  B01J2219/00378 ,  B01J2219/00527 ,  B01J2219/00677 ,  B01L3/0268 ,  B01L2200/143 ,  B01L2200/148 ,  B01L2300/0819 ,  B01L2400/0436 ,  B01L2400/0439 ,  B41J2/04541 ,  B41J2/04563 ,  B41J2/04575 ,  B41J2/0458 ,  B41J2/14008

摘要： The invention provides apparatuses and methods for acoustically ejecting the fluid from a reservoir contained in or disposed on a substrate. The reservoir has a portion adapted to contain a fluid, and an acoustic radiation generator is positioned in acoustic coupling relationship to the reservoir. Acoustic radiation generated by the acoustic radiation generator is transmitted through at least the portion of the reservoir to an analyzer. The analyzer is capable of determining the energy level of the transmitted acoustic radiation and raising the energy level of subsequent pulses to a level sufficient to eject fluid droplets from the reservoir. The invention is particularly suited for delivering fluid from a plurality of reservoirs in an accurate and efficient manner.

摘要翻译： 本发明提供了用于从包含在或设置在基板上的储存器中喷射流体的装置和方法。 储存器具有适于容纳流体的部分，并且声辐射发生器被定位成与储存器的声耦合关系。 由声辐射发生器产生的声辐射通过至少一部分储存器传输到分析器。 分析仪能够确定所发射的声辐射的能量水平，并且将后续脉冲的能量水平提高到足以从储存器喷射流体液滴的水平。 本发明特别适用于以准确和有效的方式从多个储层输送流体。

公开(公告)号：US20090007676A1

公开(公告)日：2009-01-08

申请号：US12187122

申请日：2008-08-06

申请人： Richard N. Ellson ,  Richard G. Stearns

发明人： Richard N. Ellson ,  Richard G. Stearns

IPC分类号： G01N29/024

CPC分类号： B01L3/0268 ,  B01J2219/00362 ,  B01J2219/00702 ,  B01L2200/143 ,  B01L2300/0819 ,  B01L2400/0436 ,  B01L2400/0439 ,  C40B60/14 ,  G01F23/296 ,  G01F23/2968 ,  G01N29/02 ,  G01N29/22 ,  G01N29/222 ,  G01N29/28 ,  G01N35/1016 ,  G01N2035/00158 ,  G01N2291/02818 ,  G01N2291/02836 ,  G01N2291/02881 ,  G01N2291/2695

摘要： The invention provides devices and methods for acoustically determining the properties of the contents of one or more reservoirs in a plurality of reservoirs. Each reservoir is adapted to contain a fluid. An acoustic radiation generator can be positioned in acoustic coupling relationship to each of the reservoirs. Acoustic radiation generated by the acoustic radiation generator is transmitted through each reservoir to an analyzer. The analyzer is capable of analyzing a characteristic of the transmitted acoustic radiation and optionally correlating the characteristic to a property of the reservoirs' contents. Properties that may be determined include volume, temperature, and composition. The invention is particularly suited to determining the properties of the contents of a plurality of reservoirs to allow for accuracy and control over the dispensing of fluids therefrom.

摘要翻译： 本发明提供用于声学确定多个储存器中的一个或多个储存器的内容物的性质的装置和方法。 每个储存器适于容纳流体。 声辐射发生器可以定位成与每个储存器的声耦合关系。 由声辐射发生器产生的声辐射通过每个储存器传送到分析器。 分析仪能够分析所发射的声辐射的特性，并且可选地将特征与储层内容物性相关联。 可以确定的属性包括体积，温度和组成。 本发明特别适合于确定多个储存器的内容物的性质，以允许精确度和控制从其中分配流体。

公开(公告)号：US20080173077A1

公开(公告)日：2008-07-24

申请号：US12079173

申请日：2008-03-24

申请人： Richard N. Ellson ,  Mitchell W. Mutz ,  Shehrzad Ahmed Qureshi ,  Richard G. Stearns

发明人： Richard N. Ellson ,  Mitchell W. Mutz ,  Shehrzad Ahmed Qureshi ,  Richard G. Stearns

IPC分类号： G01N29/024

CPC分类号： G01N29/4418 ,  B01J2219/00362 ,  B01J2219/00702 ,  B01L3/0268 ,  B01L2200/143 ,  B01L2300/0819 ,  B01L2300/0829 ,  B01L2300/1888 ,  B01L2400/0436 ,  B01L2400/0439 ,  B41J2/14008 ,  C40B60/14 ,  G01F23/296 ,  G01F23/2968 ,  G01N29/02 ,  G01N29/024 ,  G01N29/22 ,  G01N29/222 ,  G01N29/28 ,  G01N29/4427 ,  G01N29/46 ,  G01N35/1016 ,  G01N2035/00158 ,  G01N2291/02818 ,  G01N2291/02836 ,  G01N2291/02881 ,  G01N2291/2695

摘要： Methods are provided for analyzing characteristics of fluids in the context of an acoustic ejection system. Such a system has a controller, an acoustic radiation generator, and a coupling medium coupling the radiation to a reservoir holding fluid. The methods can use acoustic radiation to both perturb a surface of the fluid in the reservoir and analyze the effect of the perturbation. The methods may use information about prior fluids. The methods of the invention can determine physical characteristics such as speed of sound and viscosity. The methods also include ways to determine a level of acoustic energy suitable to eject a droplet. Preferably the methods are executed automatically under control of programming of a controller of an acoustic ejection system.

公开(公告)号：US20070034104A1

公开(公告)日：2007-02-15

申请号：US10546167

申请日：2003-12-16

申请人： Howard Manning ,  Benjamin Mitchell

发明人： Howard Manning ,  Benjamin Mitchell

IPC分类号： B41L3/02

CPC分类号： B82Y30/00 ,  B01J19/0046 ,  B01J2219/00362 ,  B01J2219/00378 ,  B01J2219/00385 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00612 ,  B01J2219/00659 ,  B01J2219/00675 ,  B01J2219/00689 ,  B01J2219/00722 ,  B41J2/2132 ,  C40B40/06 ,  C40B50/14 ,  C40B60/14 ,  G01N35/1067

摘要： A printing method and apparatus is disclosed wherein the printhead has a linear array of nozzles oriented in an axis, and drops of a liquid are printed at a particular location from first and second nozzles of the printhead at a first location. In an embodiment, the nozzles are arranged in groups, each group of nozzles being used to superpose spots of a particular liquid at a different location. A further method is described wherein the printhead is triggered by transferring printing triggers from a processing module to a printhead at print intervals representative of a plurality of fixed units of spatial displacement of the printhead. The present invention is particularly advantageous for the cycling of print nozzles, superposition of spots in linear arrays and fine control of spot positioning. The invention has particular application in the field of printing DNA microarrays.

摘要翻译： 公开了一种打印方法和装置，其中打印头具有沿轴线定向的喷嘴的线性阵列，并且在第一位置处，打印头的第一和第二喷嘴在特定位置处印刷液体。 在一个实施例中，喷嘴被分组布置，每组喷嘴用于在不同位置叠加特定液体的斑点。 描述了另一种方法，其中通过以打印间隔将打印触发从处理模块传送到打印头来触发打印头，该打印间隔代表打印头的空间位移的多个固定单元。 本发明特别有利于打印喷嘴的循环，线性阵列中的斑点的叠加以及斑点定位的精细控制。 本发明在印刷DNA微阵列领域具有特殊的应用。

公开(公告)号：US20030203505A1

公开(公告)日：2003-10-30

申请号：US10402695

申请日：2003-03-28

发明人： Roger O. Williams ,  Tarlochan Singh ,  N. Nicolas Mansour ,  Lawrence Lee JR. ,  Michael J. Forbush

IPC分类号： G01N001/10 ,  C12Q001/68 ,  G01N033/543

CPC分类号： B01L3/0268 ,  B01J19/0046 ,  B01J2219/00362 ,  B01J2219/00378 ,  B01J2219/00527 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00612 ,  B01J2219/00637 ,  B01J2219/00641 ,  B01J2219/00659 ,  B01J2219/00725 ,  B01L2300/0819 ,  B01L2400/0433 ,  B01L2400/0436 ,  B05B17/0615 ,  C40B30/04 ,  C40B40/02 ,  C40B40/04 ,  C40B40/08 ,  C40B40/10 ,  C40B50/00 ,  C40B60/14 ,  F04F7/00 ,  G01N21/11 ,  Y10T137/206 ,  Y10T137/2196 ,  Y10T436/2575

摘要： Acoustic waves are used to transfer small amounts of fluid in a non-contact manner. Acoustic waves are propagated through a pool of a source fluid in such a manner that causes the ejection of a single micro-droplet from the surface of the pool. The droplet is ejected towards a target with sufficient force to provide for contact of the droplet with the target. Because the fluid is not contacted by any fluid transfer device such as a pipette, the opportunities for contamination are minimized. Methods may be employed to transfer fluids from an array of source sites to an array of target sites, thereby enabling the precise automation of a wide variety of procedures including screening and synthesis procedures commonly used in biotechnology.

摘要翻译： 声波用于以非接触方式转移少量流体。 声波以这样的方式传播通过源流体池，使得从池的表面喷射单个微滴。 液滴以足够的力朝向目标喷射以提供液滴与靶的接触。 由于流体不被诸如移液管之类的任何流体转移装置接触，污染的机会被最小化。 可以采用方法将流体从源位置阵列转移到靶位点阵列，从而能够精确自动化各种程序，包括通常用于生物技术的筛选和合成过程。

公开(公告)号：US20240208210A1

公开(公告)日：2024-06-27

申请号：US18531501

申请日：2023-12-06

申请人： Labcyte Inc.

发明人： Sammy S. Datwani ,  Carson Riche ,  Marsha N. Blauwkamp ,  Justin Blonigan

IPC分类号： B41J2/045 ,  B01J19/00 ,  B01J19/26 ,  B01L3/02

CPC分类号： B41J2/04575 ,  B01J19/0046 ,  B01J19/26 ,  B01L3/0268 ,  B41J2/04516 ,  B41J2/04588 ,  B01J2219/00362 ,  B01L2200/0647 ,  B01L2200/148 ,  B01L2400/0436 ,  B01L2400/0439

摘要： Methods of ejecting droplets containing a non-Newtonian fluid by an acoustic droplet ejector can include applying a tone burst of focused acoustic energy to a fluid reservoir containing a non-Newtonian fluid at sufficient amplitude to effect droplet ejection according to a tone burst pattern. The tone burst pattern may include three discrete tone burst segments, the first tone burst segment having greater duration than the second and third segments, and third segment having greater duration than the second segment. The exact durations and amplitudes of the tone burst segments can be tuned to influence the ejection properties.