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28 条记录 (耗时 0.017 秒)

    Method and instruments for non-invasive analyte measurement
    1.
    发明授权
    Method and instruments for non-invasive analyte measurement 失效
    用于非侵入性分析物测量的方法和仪器

    公开(公告)号：US06958039B2

    公开(公告)日：2005-10-25

    申请号：US10824254

    申请日：2004-04-14

    申请人： John F. Burd ,  Gary Krantz ,  William Sell

    发明人： John F. Burd ,  Gary Krantz ,  William Sell

    IPC分类号： A61B3/117 ,  A61B5/00

    CPC分类号： A61B5/1455 ,  A61B3/10 ,  A61B3/117 ,  A61B5/14532 ,  A61B5/14546

    摘要： The present invention is related to optical non-invasive methods and instruments to detect the level of analyte concentrations in the tissue of a subject. The spectra of mid-infrared radiation emitted from a subject's body are altered corresponding to the concentration of various compounds within the radiating tissue. In one aspect of the invention, an instrument measures the level of mid-infrared radiation from the subject's body surface, such as the eye, and determines a specific analyte's concentration based on said analyte's distinctive mid-infrared radiation signature.

    摘要翻译： 本发明涉及用于检测受试者组织中分析物浓度水平的光学非侵入性方法和仪器。 从受试者身体发射的中红外辐射的光谱根据辐射组织内各种化合物的浓度而改变。 在本发明的一个方面中，仪器测量来自对象的身体表面（例如眼睛）的中红外辐射的水平，并且基于所述分析物的特征性中红外辐射特征确定特定分析物的浓度。

    Luminescent substrate preparation and its use in specific binding assays
    4.
    发明授权
    Luminescent substrate preparation and its use in specific binding assays 失效
    发光底物制备及其在特异性结合测定中的应用

    公开(公告)号：US4743541A

    公开(公告)日：1988-05-10

    申请号：US756039

    申请日：1985-07-17

    申请人： Keith W. Higgins ,  Christopher R. Brown ,  John F. Burd

    发明人： Keith W. Higgins ,  Christopher R. Brown ,  John F. Burd

    IPC分类号： G01N21/78 ,  C07D237/32 ,  C09K11/06 ,  G01N21/76 ,  G01N33/533 ,  C07D237/30 ,  G01N33/535 ,  G01N33/543

    CPC分类号： C07D237/32 ,  G01N33/533 ,  Y10S435/81 ,  Y10S435/962 ,  Y10S435/968 ,  Y10S436/805

    摘要： A luminescent substrate preparation having a concentration of catalytic inhibitors of less than about 100 ppm. The preparation is obtained by heating commercial grade luminol in a basic solution, crystallizing the luminol and separating the luminol crystals from the boiled solution. The heating, crystallization and separation steps are preferably repeated sequentially at least four times, with the starting material for each sequence after the first being the luminol preparation produced in the previous sequence. The luminol preparation has an enhanced pattern of activity, in that light output is substantially constant over a period of at least about one hour, with the intensity of light emitted by the preparation being at least about ten times that of commercially available luminol. Because of these enhanced characteristics, the luminol preparation is particularly adapted for use as a tag in specific binding assays where the concentration of analyte to be detected is low.

    摘要翻译： 具有小于约100ppm的催化抑制剂浓度的发光底物制剂。 该制剂通过在碱性溶液中加热商业级鲁米诺，结晶鲁米诺和从煮沸的溶液中分离鲁米诺晶体而获得。 加热，结晶和分离步骤优选顺序地重复至少四次，其中首先是在先前序列中产生的鲁米诺制剂之后的每个序列的起始材料。 鲁米诺制剂具有增强的活性模式，因为光输出在至少约1小时的时间内基本上是恒定的，制剂发出的光的强度至少是市售的鲁米诺的十倍。 由于这些增强的特征，鲁米诺制剂特别适用于特异性结合测定中的标签，其中待检测的分析物的浓度低。

    Homogeneous specific binding assay employing an intramolecularly modulated photogenic enzyme substrate label
    8.
    发明授权
    Homogeneous specific binding assay employing an intramolecularly modulated photogenic enzyme substrate label 失效
    使用分子内调节的光致酶底物标签的均相特异性结合测定

    公开(公告)号：US4318981A

    公开(公告)日：1982-03-09

    申请号：US143497

    申请日：1980-04-24

    申请人： John F. Burd ,  Thomas M. Li

    发明人： John F. Burd ,  Thomas M. Li

    IPC分类号： G01N33/58 ,  C12Q1/66

    CPC分类号： G01N33/581 ,  Y10S435/971

    摘要： A homogeneous specific binding assay method and reagent means for determining a ligand, such as a hapten, antigen or antibody, in, or the ligand binding capacity of, a liquid medium employing a labeled conjugate which upon enzymatic cleavage produces a detectable indicator product. The improvement comprises employing as the label component in the labeled conjugate, a residue having the formula:P--X--M--Rwherein P is a photophore, e.g., a fluorescer, which emits light upon exposure to excitation means, X is an enzymatically cleavable linkage, M is a modulator, e.g., a quencher, for said light emission of the photophore, and R is a linking group to the binding component, e.g., the ligand or analog thereof, in the labeled conjugate. The labeled conjugate and the cleaved indicator product emit substantially different amounts of light upon excitation due to modulation of the emission of photophore P by the proximity of modulator M in the labeled conjugate.

    摘要翻译： 使用标记的缀合物的液体培养基中的配体，例如半抗原，抗原或抗体或其配体结合能力的均相特异性结合测定方法和试剂方法，其在酶裂解时产生可检测的指示剂产物。 改进包括在标记的缀合物中使用标记成分，具有下式的残基：PXMR，其中P是光致抗原，例如荧光素，其在暴露于激发装置时发光，X是可酶切割的键，M是 调节剂，例如猝灭剂，用于所述光致发光的发光，R是与标记的缀合物中的结合组分（例如其配体或类似物）的连接基团。 标记的缀合物和切割的指示剂产物由于通过调节剂M在标记的缀合物中的接近而调制光致磷P的发射而在激发时发射基本上不同的光量。

    .beta.Galactosyl-umbelliferone valproic acid conjugates
    9.
    发明授权
    .beta.Galactosyl-umbelliferone valproic acid conjugates 失效
    β半乳糖基 - 伞形酮丙戊酸共轭物

    公开(公告)号：US4292425A

    公开(公告)日：1981-09-29

    申请号：US139716

    申请日：1980-04-14

    申请人： Robert T. Buckler ,  John F. Burd ,  Raphael C. Wong

    发明人： Robert T. Buckler ,  John F. Burd ,  Raphael C. Wong

    IPC分类号： C07H17/07 ,  C07K16/44 ,  G01N33/94 ,  C07H15/22 ,  C07H17/04

    CPC分类号： C07K16/44 ,  C07H17/07 ,  G01N33/9473

    摘要： Reagents for use in binding assays, particularly immunoassays, to determine valproic acid in liquid media such as serum. Such reagents include antibody to valproic acid and .beta.-galactosyl-umbelliferone-valproic acid conjugates. Also provided are compounds used to prepare such reagents, including valproic acid immunogen conjugates and intermediates in the synthesis of such immunogen conjugates and .beta.-galactosyl-umbelliferone-labeled conjugates.

    摘要翻译： 用于结合测定法的试剂，特别是免疫测定法，用于测定液体培养基如血清中的丙戊酸。 这些试剂包括丙戊酸抗体和β-半乳糖基 - 伞形酮 - 丙戊酸缀合物。 还提供了用于制备此类试剂的化合物，包括丙戊酸免疫原缀合物和合成此类免疫原缀合物和β-半乳糖基 - 伞形酮标记的缀合物的中间体。

    Methods of determining initiation and variable end points for measuring a chemical reaction
    10.
    发明授权
    Methods of determining initiation and variable end points for measuring a chemical reaction 失效
    确定用于测量化学反应的起始点和可变终点的方法

    公开(公告)号：US5885839A

    公开(公告)日：1999-03-23

    申请号：US842616

    申请日：1997-04-15

    申请人： Paul J. Lingane ,  John F. Burd ,  Karen A. Goins ,  Michael D. Goins

    发明人： Paul J. Lingane ,  John F. Burd ,  Karen A. Goins ,  Michael D. Goins

    IPC分类号： G01N21/86 ,  G01N33/00

    CPC分类号： G01N21/8483 ,  Y10T436/112499 ,  Y10T436/115831 ,  Y10T436/142222 ,  Y10T436/143333 ,  Y10T436/144444

    摘要： The present invention provides a method of using a reflectance-reading device to determine an initiation time point for measuring the chemical reaction of an analyte from a biological liquid sample on a test surface. The initiation time point is determined by using a device to read a reflectance of a test surface at a plurality of time points, and calculating the K/S ratio of the test surface at each time point according to the Kubelka-Munk equation. As the device continues to calculate a K/S ratio for each time point, the device monitors the rate of change of the K/S ratio with respect to time. The device then determines the initiation time point to be when the rate of change of the K/S ratio is maximal. The present invention also provides a method of measuring the concentration of an analyte on a test surface. The concentration is measured by determining an initiation time point according to the method described above and then measuring the concentration of the analyte at a variable end point. The variable end point is essentially determined to be the time at which the rate of change of a concentration parameter is less than a threshold value.

    摘要翻译： 本发明提供一种使用反射率读取装置来确定用于测量来自测试表面上的生物液体样品的分析物的化学反应的起始时间点的方法。 通过使用设备读取多个时间点的测试表面的反射率并根据Kubelka-Munk方程计算每个时间点的测试表面的K / S比来确定起始时间点。 当设备继续计算每个时间点的K / S比时，设备将监视K / S比率相对于时间的变化率。 然后，当K / S比的变化率最大时，该装置确定起始时间点。 本发明还提供了一种测量测试表面上分析物浓度的方法。 通过根据上述方法确定起始时间点，然后测量分析物在可变终点的浓度来测量浓度。 可变终点基本上被确定为浓度参数的变化率小于阈值的时间。