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公开(公告)号：US20110054193A1
公开(公告)日：2011-03-03
申请号：US12989540
申请日：2009-04-28
CPC分类号： A61K31/366 , C12N9/0006 , C12P7/42 , C12P7/62 , C12P17/06
摘要： The present invention relates to a polypeptide with HMG-CoA reductase activity, to its polynucleotide congener and to a method for the production of a statin comprising over expression of said polypeptide.
摘要翻译： 本发明涉及具有HMG-CoA还原酶活性的多肽，其多核苷酸同系物和包含过表达所述多肽的他汀类药物的制备方法。
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公开(公告)号：US20090197311A1
公开(公告)日：2009-08-06
申请号：US12304283
申请日：2007-06-18
CPC分类号： C12P17/06
摘要： The present invention provides a fermentative process for the synthesis of simvastatin by providing a host capable of incorporating the 2,2-dimethylbutyrate side chain into simvastatin, i.e. by customizing a polyketide synthase gene optimized for synthesis and/or incorporation of 2,2-dimethylbutyrate; optionally feeding said host with the appropriate substrate for 2,2-dimethylbutyrate synthesis; fermenting said host to obtain simvastatin or analogues or derivatives thereof, i.e. by producing simvastatin on an industrial scale by a fed-batch process.
摘要翻译： 本发明提供了一种通过提供能够将2,2-二甲基丁酸酯侧链掺入辛伐他汀的宿主来合成辛伐他汀的发酵方法，即通过定制优化用于合成和/或并入2,2-二甲基丁酸酯的聚酮化合物合酶基因 ; 任选地向所述主体供应合适的用于2,2-二甲基丁酸酯合成的底物; 发酵所述宿主以获得辛伐他汀或其类似物或衍生物，即通过补料分批方法以工业规模生产辛伐他汀。
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公开(公告)号：US20110223640A1
公开(公告)日：2011-09-15
申请号：US13119764
申请日：2009-09-21
CPC分类号： C12N15/52 , C07K14/385 , C12P7/42 , C12P17/06
摘要： The present invention provides a method for the fermentative production of compactin, lovastatin, pravastatin or simvastatin comprising culturing a host, preferably a filamentous fungus, comprising the polynucleotide of the lovE transcription regulator gene from Aspergillus terreus. Furthermore, the invention provides a host for the production of above mentioned statines comprising the polynucleotide of the lovE transcription regulator gene from Aspergillus terreus.
摘要翻译： 本发明提供了发酵生产美伐他汀，洛伐他汀，普伐他汀或辛伐他汀的方法，包括培养宿主，优选丝状真菌，其包含来自土曲霉的lovE转录调节基因的多核苷酸。 此外，本发明提供了一种用于生产上述托胺的宿主，其包含来自土曲霉的lovE转录调节基因的多核苷酸。
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公开(公告)号：US20100217032A1
公开(公告)日：2010-08-26
申请号：US12518713
申请日：2007-12-11
申请人： Paul Klaassen , Adrianus Wilhelmus Hermanus Vollebregt , Marco Alexander Van Den Berg , Marcus Hans , Jan Metske Van Der Laan
发明人： Paul Klaassen , Adrianus Wilhelmus Hermanus Vollebregt , Marco Alexander Van Den Berg , Marcus Hans , Jan Metske Van Der Laan
IPC分类号： C12P7/62 , C07K14/195 , C07H21/04 , C07C69/34
CPC分类号： C12P7/42 , C12N9/0077 , C12P7/62
摘要： The present invention provides a polypeptide having an amino acid sequence according to SEQ ID NO 3, SEQ ID NO 6 or SEQ ID NO 43-59. The present invention also provides a polynucleotide comprising a DNA sequence encoding these polypeptides and a method for isolating polynucleotides encoding polypeptides capable of improving the compactin into pravastatin conversion. Furthermore, the present invention provides a method for producing pravastatin and a pharmaceutical composition comprising pravastatin.
摘要翻译： 本发明提供具有根据SEQ ID NO 3，SEQ ID NO 6或SEQ ID NO 43-59的氨基酸序列的多肽。 本发明还提供了一种包含编码这些多肽的DNA序列的多核苷酸，以及一种分离编码多肽的方法，所述多核苷酸能够将该致密蛋白改进普伐他汀转化。 此外，本发明提供了普伐他汀的制造方法和含有普伐他汀的药物组合物。
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5.发明授权Recombinant host cell with deficiency in non-ribosomal peptide synthase production 有权具有非核糖体肽合成酶生产缺陷的重组宿主细胞公开(公告)号：US08945898B2
公开(公告)日：2015-02-03
申请号：US13806128
申请日：2011-07-01
申请人： Noel Nicolaas Maria Elisabeth Van Peij , Marcus Hans , Martina Beishuizen , Dick Schipper , Robertus Antonius Mijndert Van Der Hoeven , Olaf Leonardus Schouten
发明人： Noel Nicolaas Maria Elisabeth Van Peij , Marcus Hans , Martina Beishuizen , Dick Schipper , Robertus Antonius Mijndert Van Der Hoeven , Olaf Leonardus Schouten
摘要： The present invention relates to a method for the production of a compound of interest by microbial fermentation, wherein the microbial host cell used has been modified in its genome such that it results in a deficiency in the production of at least one non-ribosomal peptide synthase. The present invention further relates to a microbial host cell that has been modified in its genome such that it results in a deficiency in the production of at least one non-ribosomal peptide synthase. The invention further relates to a compound of interest.
摘要翻译： 本发明涉及通过微生物发酵生产感兴趣的化合物的方法，其中使用的微生物宿主细胞在其基因组中已被修饰，从而导致至少一种非核糖体肽合成酶的产生缺陷 。 本发明还涉及在其基因组中已被修饰的微生物宿主细胞，从而导致至少一种非核糖体肽合酶的产生缺陷。 本发明还涉及目的化合物。
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公开(公告)号：US20130144034A1
公开(公告)日：2013-06-06
申请号：US13806128
申请日：2011-07-01
申请人： Noel Nicolaas Maria Elisabeth Van Peij , Marcus Hans , Martina Beishuizen , Dick Schipper , Robertus Antonius Mijndert Van Der Hoeven , Olaf Leonardus Schouten
发明人： Noel Nicolaas Maria Elisabeth Van Peij , Marcus Hans , Martina Beishuizen , Dick Schipper , Robertus Antonius Mijndert Van Der Hoeven , Olaf Leonardus Schouten
IPC分类号： C12P21/02
摘要： The present invention relates to a method for the production of a compound of interest by microbial fermentation, wherein the microbial host cell used has been modified in its genome such that it results in a deficiency in the production of at least one non-ribosomal peptide synthase. The present invention further relates to a microbial host cell that has been modified in its genome such that it results in a deficiency in the production of at least one non-ribosomal peptide synthase. The invention further relates to a compound of interest.
摘要翻译： 本发明涉及通过微生物发酵生产感兴趣的化合物的方法，其中使用的微生物宿主细胞在其基因组中已被修饰，从而导致至少一种非核糖体肽合成酶的产生缺陷 。 本发明还涉及在其基因组中已被修饰的微生物宿主细胞，从而导致至少一种非核糖体肽合酶的产生缺陷。 本发明还涉及目的化合物。
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公开(公告)号：US20100009404A1
公开(公告)日：2010-01-14
申请号：US12444108
申请日：2007-10-02
CPC分类号： C12N9/0004
摘要： The present invention describes a process for the production of an N-α-amino-hydroxyphenylacetyl or an N-α-aminophenylacetyl β-lactam antibiotic comprising an IPNS-catalysed conversion of a precursor tripeptide hydroxyphenylglycyl-cysteinyl-valine (HpgCV) or phenylglycyl-cysteinyl-valine (PgCV), respectively, to the N-hydroxyphenylglycyl or the N-phenylglycyl β-lactam antibiotic, respectively. The tripeptide HpgCV or the tripeptide PgCV may further be prepared by contacting the amino acids hydroxyphenylglycine (Hpg) or phenylglycine (Pg), cystein (C) and valine (V) with a non-ribosomal peptide synthetase (NRPS) to effect formation of the tripeptide HpgCV or the tripeptide PgCV, the NRPS comprising a first module M1 specific for Hpg or Pg, a second module M2 specific for C and a third module M3 specific for V An IPNS is further provided having an improved activity in this conversion, as well as an NRPS catalysing the formation of the tripeptides. Also a host cell is provided capable of fermentatively producing β-lactam antibiotics with N-α-amino-hydroxyphenylacetyl or an N-α-aminophenylacetyl side chains.
摘要翻译： 本发明描述了制备N-α-氨基 - 羟基苯基乙酰基或N-α-氨基苯基乙酰基β-内酰胺抗生素的方法，其包括前体三肽羟基苯基甘氨酰 - 半胱氨酰 - 缬氨酸（HpgCV）或苯基甘氨酰 - 半胱氨酰缬氨酸（PgCV）分别分别与N-羟基苯基甘氨酰或N-苯基甘氨酰β-内酰胺抗生素。 三肽HpgCV或三肽PgCV可以通过使氨基酸羟基苯基甘氨酸（Hpg）或苯基甘氨酸（Pg），半胱氨酸（C）和缬氨酸（V）与非核糖体肽合成酶（NRPS）接触来进一步制备，以形成 三肽HpgCV或三肽PgCV，NRPS包括特定于Hpg或Pg的第一模块M1，特定于C的第二模块M2和特定于V An IPNS的第三模块M3，还具有在该转换中具有改进的活性 作为催化三肽形成的NRPS。 还提供了能够用N-α-氨基 - 羟基苯基乙酰基或N-α-氨基苯乙酰侧链发酵生产β-内酰胺抗生素的宿主细胞。
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公开(公告)号：US08293511B2
公开(公告)日：2012-10-23
申请号：US12444108
申请日：2007-10-02
CPC分类号： C12N9/0004
摘要： The present invention describes a process for the production of an N-α-amino-hydroxyphenylacetyl or an N-α-aminophenylacetyl β-lactam antibiotic comprising an IPNS-catalysed conversion of a precursor tripeptide hydroxyphenylglycyl-cysteinyl-valine (HpgCV) or phenylglycyl-cysteinyl-valine (PgCV), respectively, to the N-hydroxyphenylglycyl or the N-phenylglycyl β-lactam antibiotic, respectively. The tripeptide HpgCV or the tripeptide PgCV may further be prepared by contacting the amino acids hydroxyphenylglycine (Hpg) or phenylglycine (Pg), cystein (C) and valine (V) with a non-ribosomal peptide synthetase (NRPS) to effect formation of the tripeptide HpgCV or the tripeptide PgCV, the NRPS comprising a first module M1 specific for Hpg or Pg, a second module M2 specific for C and a third module M3 specific for V An IPNS is further provided having an improved activity in this conversion, as well as an NRPS catalysing the formation of the tripeptides. Also a host cell is provided capable of fermentatively producing β-lactam antibiotics with N-α-amino-hydroxyphenylacetyl or an N-α-aminophenylacetyl side chains.
摘要翻译： 本发明描述了一种制备N-α-氨基 - 羟基苯基乙酰基或N-α-氨基苯基乙酰基 - 内酰胺抗生素的方法，其包括前体三肽羟基苯基甘氨酰 - 半胱氨酰 - 缬氨酸（HpgCV）或苯基甘氨酰 - 半胱氨酰缬氨酸（PgCV）分别转化为N-羟基苯基甘氨酰或N-苯基甘氨酰和β-内酰胺抗生素。 三肽HpgCV或三肽PgCV可以通过使氨基酸羟基苯基甘氨酸（Hpg）或苯基甘氨酸（Pg），半胱氨酸（C）和缬氨酸（V）与非核糖体肽合成酶（NRPS）接触来进一步制备，以形成 三肽HpgCV或三肽PgCV，NRPS包括特定于Hpg或Pg的第一模块M1，特定于C的第二模块M2和特定于V An IPNS的第三模块M3，还具有在该转换中具有改进的活性 作为催化三肽形成的NRPS。 还提供了能够用N-α-氨基 - 羟基苯基乙酰基或N-α-氨基苯乙酰侧链发酵生产β-内酰胺抗生素的宿主细胞。
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